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The map label for this gene is mcl1 [H]

Identifier: 13471372

GI number: 13471372

Start: 1109744

End: 1110700

Strand: Direct

Name: mcl1 [H]

Synonym: mlr1323

Alternate gene names: 13471372

Gene position: 1109744-1110700 (Clockwise)

Preceding gene: 13471371

Following gene: 13471373

Centisome position: 15.77

GC content: 64.99

Gene sequence:

>957_bases
ATGAGCCACACCATCAACCATTTGAAAAAATTGCGGCTGCAGCGCAGCGAGCTGGCGGTTCCCGGTTCCAGCCCGGAGAT
GATCGACAAGGCGGCCAACAGCGCCGCCGACTTCGTCTTCCTCGACATCGAGGACGCGGTCGCCCCGCCCGACAAGGAAC
GCGCCCGCAAGAACATCATCCAGGCGCTGAACGACATCGACTGGCGTGCCATGGGCAAGACCGTCTCGGTGCGCATCAAT
GGGCTCGACACCCATTACATGTACCGCGACGTGGTCGACGTCATGGAACAGGCCGGCGACCGGCTGGACACGATCCTCGT
GCCAAAGGTCGGCGTGCCGGCCGACCTCTACATGGTCGAGGCCATGGTCAACCAGATCGAGATGGCCAAGGGTTTCAAGA
CCCGTGTCGGCTTGGAAGCGCTGATCGAAACAGCGCTCGGCATGGCCAATGTCGAAGCGATTGCCGCCACTCCCGGCCGG
CTGGAAGCCATGCATTTCGGCGTCGCCGACTATGCGGCGAGTTGCAAGGCGCGCACCGTCAACATTGGCGGCCTCAACCC
GGACTATCCCGGCGACCAGTGGCATTTCGCGCTATCGCGCATGACGGTCGCCTGCCGCGCCTATGGCCTGCGCGCCATTG
ACGGGCCATTCGGCGATTTCTCCGATCCGGAGGGCTACATCGCCGGCGCCAGGCGCGCCGCGGCCCTCGGCATCGAAGGC
AAATGGGCGATCCACCCGTCGCAGATCGCGCTCGCCAACGACGTCTTCTCGCCGCCGGAAAAGGAGGTCGCGCGGGCCAG
GCGCATCCTTGAGGTGCTCAAGGAAGCCGAGGCCCTGGGCAAAGGCGCCGCCGCACTCGACGGCAAGATGATCGACGCCG
CGTCGGAACGCATGGCGAGGAACGTCCTGGTGGTCAACGAGGCCATCGAACGCGCCGGCCAAACCGCCACGCATTGA

Upstream 100 bases:

>100_bases
GCGTGCGGCTGGCGCCGGGCTCCGGCGTCGGCGCCGCCATCCAGCATTTCCGCGCCTCGGCCGCCGTGCCGGTCGCCGAA
GCCGCCTGAGGGAGCCGACG

Downstream 100 bases:

>100_bases
CTGTTCAGGGAGGACGACAATGGACATTCACGAATATCAGGCCAAGGAACTGCTCGCCCGGCACGGCGTGCATGTGCCGC
GCGGCGGGCTGGCCTACAGC

Product: beta subunit of citrate lyase

Products: NA

Alternate protein names: (3S)-malyl-CoA/beta-methylmalyl-CoA lyase; L-malyl-CoA/beta-methylmalyl-CoA lyase [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MSHTINHLKKLRLQRSELAVPGSSPEMIDKAANSAADFVFLDIEDAVAPPDKERARKNIIQALNDIDWRAMGKTVSVRIN
GLDTHYMYRDVVDVMEQAGDRLDTILVPKVGVPADLYMVEAMVNQIEMAKGFKTRVGLEALIETALGMANVEAIAATPGR
LEAMHFGVADYAASCKARTVNIGGLNPDYPGDQWHFALSRMTVACRAYGLRAIDGPFGDFSDPEGYIAGARRAAALGIEG
KWAIHPSQIALANDVFSPPEKEVARARRILEVLKEAEALGKGAAALDGKMIDAASERMARNVLVVNEAIERAGQTATH

Sequences:

>Translated_318_residues
MSHTINHLKKLRLQRSELAVPGSSPEMIDKAANSAADFVFLDIEDAVAPPDKERARKNIIQALNDIDWRAMGKTVSVRIN
GLDTHYMYRDVVDVMEQAGDRLDTILVPKVGVPADLYMVEAMVNQIEMAKGFKTRVGLEALIETALGMANVEAIAATPGR
LEAMHFGVADYAASCKARTVNIGGLNPDYPGDQWHFALSRMTVACRAYGLRAIDGPFGDFSDPEGYIAGARRAAALGIEG
KWAIHPSQIALANDVFSPPEKEVARARRILEVLKEAEALGKGAAALDGKMIDAASERMARNVLVVNEAIERAGQTATH
>Mature_317_residues
SHTINHLKKLRLQRSELAVPGSSPEMIDKAANSAADFVFLDIEDAVAPPDKERARKNIIQALNDIDWRAMGKTVSVRING
LDTHYMYRDVVDVMEQAGDRLDTILVPKVGVPADLYMVEAMVNQIEMAKGFKTRVGLEALIETALGMANVEAIAATPGRL
EAMHFGVADYAASCKARTVNIGGLNPDYPGDQWHFALSRMTVACRAYGLRAIDGPFGDFSDPEGYIAGARRAAALGIEGK
WAIHPSQIALANDVFSPPEKEVARARRILEVLKEAEALGKGAAALDGKMIDAASERMARNVLVVNEAIERAGQTATH

Specific function: Catalyzes the reversible condensation of glyoxylate and acetyl-CoA to L-malyl-CoA and the reversible condensation of glyoxylate and propionyl-CoA to beta-methylmalyl-CoA [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=305, Percent_Identity=29.8360655737705, Blast_Score=129, Evalue=4e-30,
Organism=Escherichia coli, GI87081764, Length=296, Percent_Identity=31.7567567567568, Blast_Score=119, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17557458, Length=310, Percent_Identity=28.0645161290323, Blast_Score=111, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.3.24 [H]

Molecular weight: Translated: 34435; Mature: 34304

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHTINHLKKLRLQRSELAVPGSSPEMIDKAANSAADFVFLDIEDAVAPPDKERARKNII
CCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEEEHHCCCCCCHHHHHHHHH
QALNDIDWRAMGKTVSVRINGLDTHYMYRDVVDVMEQAGDRLDTILVPKVGVPADLYMVE
HHHHCCCHHHCCCEEEEEEECCCHHHHHHHHHHHHHHCCCHHCEEEECCCCCCHHHHHHH
AMVNQIEMAKGFKTRVGLEALIETALGMANVEAIAATPGRLEAMHFGVADYAASCKARTV
HHHHHHHHHHCHHHHHHHHHHHHHHHCHHCCHHEECCCCCHHHHHCCHHHHHHCCCEEEE
NIGGLNPDYPGDQWHFALSRMTVACRAYGLRAIDGPFGDFSDPEGYIAGARRAAALGIEG
EECCCCCCCCCCHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCHHHHHHHHHEECCCC
KWAIHPSQIALANDVFSPPEKEVARARRILEVLKEAEALGKGAAALDGKMIDAASERMAR
EEEECCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHH
NVLVVNEAIERAGQTATH
HHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SHTINHLKKLRLQRSELAVPGSSPEMIDKAANSAADFVFLDIEDAVAPPDKERARKNII
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEEEHHCCCCCCHHHHHHHHH
QALNDIDWRAMGKTVSVRINGLDTHYMYRDVVDVMEQAGDRLDTILVPKVGVPADLYMVE
HHHHCCCHHHCCCEEEEEEECCCHHHHHHHHHHHHHHCCCHHCEEEECCCCCCHHHHHHH
AMVNQIEMAKGFKTRVGLEALIETALGMANVEAIAATPGRLEAMHFGVADYAASCKARTV
HHHHHHHHHHCHHHHHHHHHHHHHHHCHHCCHHEECCCCCHHHHHCCHHHHHHCCCEEEE
NIGGLNPDYPGDQWHFALSRMTVACRAYGLRAIDGPFGDFSDPEGYIAGARRAAALGIEG
EECCCCCCCCCCHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCHHHHHHHHHEECCCC
KWAIHPSQIALANDVFSPPEKEVARARRILEVLKEAEALGKGAAALDGKMIDAASERMAR
EEEECCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHH
NVLVVNEAIERAGQTATH
HHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA