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The map label for this gene is sucC

Identifier: 13471373

GI number: 13471373

Start: 1110720

End: 1111904

Strand: Direct

Name: sucC

Synonym: mlr1324

Alternate gene names: 13471373

Gene position: 1110720-1111904 (Clockwise)

Preceding gene: 13471372

Following gene: 13471374

Centisome position: 15.79

GC content: 64.73

Gene sequence:

>1185_bases
ATGGACATTCACGAATATCAGGCCAAGGAACTGCTCGCCCGGCACGGCGTGCATGTGCCGCGCGGCGGGCTGGCCTACAG
CCCCGAACAGGCGACCTACAGGGCGCGCGAGATCGGCGGCGGCAAATGGGTCTTGAAGGCGCAGGTTCATTCCGGCGCGC
GCGGCAAGGCCGGCGGCATAAAACTGTGCGCCAATGACGAGGAGATCTCGAGCGCCGCCGAAGCCATGCTCGGGCGCAAG
CTGGTGACGCAGCAGACCGGACCGCGCGGCAAGCTGATCTCCCGGCTCTATCTCGAGGAAGCCGTCGATATCGCCCAGGA
GCTCTATGTCGGCTTCGTGCTCGACCGCAAGGAAGAGCGCGTCATGATCGTGGCCTCGGCCGCCGGCGGCATGGAGATCG
AGGATATTGTCGAGAAAGAACCCAATTCCATCCTGCGCACATCGGTCGATCCCGGCGTCGGCATGCAGCGCTTCCAGGCG
CGCGAGATCGCCTTCGGGCTCGGCCTCGACCACAACCTGATCGGCAAGGCGACCGAAACCATCTTCAGCTGCTACCAGGT
GTTCCGCGACTACGACGCCTCGATGCTGGAGATCAATCCGCTGGTGGTGACGCGCGACGGCAATCTGATCGCGCTCGACG
CCAAGATGTCTTTCGACGAGAACGCCCTGTTCCGCCGGCCTGAAATCTCCGAGCTGCGCGACAAGAGCCAGGAAGACCCG
CGCGAGACTTTCGCCAGCGACCGCGGCCTCTCCTATGTCGGCCTCGACGGCAATATAGGCTGCATCATCAATGGAGCGGG
GCTCGCCATGGCGACAATGGACATGATCAAGATCGCCGGCGGCGAACCGGCCAACTTCCTCGACATTGGCGGCGGCGCCT
CGCCCGAGCGGGTGGCGAAATCCTTCCGTGCCGTGCTCGGCGACAAGAATGTCGAGACCATCCTGGTCAACATCTTCGCC
GGCATCAACCGCTGCGACTGGGTCGCCGAAGGCGTGATCAAGGCGATACGCGAAGTCGGCGTCAACGTGCCGCTGGTGGT
GCGTCTCTCCGGCACCAAGGCCGAGGAAGGCCGCCGCATACTGGCCGATTCCGGCGAGGCGGTGATCGTCGCCGACACGC
TGGCCGAAGCCGCCGAAAAAGCCGTCGCCGCCTGGCGCGCGGCCGCCAAGAAAAAAGCAGCCTGA

Upstream 100 bases:

>100_bases
GCCGCGTCGGAACGCATGGCGAGGAACGTCCTGGTGGTCAACGAGGCCATCGAACGCGCCGGCCAAACCGCCACGCATTG
ACTGTTCAGGGAGGACGACA

Downstream 100 bases:

>100_bases
GGGAGGTCGAGAAAATGGCAATCCTGCTCAACCGCAGCACCCGCGTCATCGTCCAGGGTTTCACCGGCAAGATCGGCAGC
TTCCACGCCGAGGACATGAA

Product: malate--CoA ligase subunit beta

Products: NA

Alternate protein names: MTK-beta; Malate thiokinase; Malyl-CoA synthetase

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MDIHEYQAKELLARHGVHVPRGGLAYSPEQATYRAREIGGGKWVLKAQVHSGARGKAGGIKLCANDEEISSAAEAMLGRK
LVTQQTGPRGKLISRLYLEEAVDIAQELYVGFVLDRKEERVMIVASAAGGMEIEDIVEKEPNSILRTSVDPGVGMQRFQA
REIAFGLGLDHNLIGKATETIFSCYQVFRDYDASMLEINPLVVTRDGNLIALDAKMSFDENALFRRPEISELRDKSQEDP
RETFASDRGLSYVGLDGNIGCIINGAGLAMATMDMIKIAGGEPANFLDIGGGASPERVAKSFRAVLGDKNVETILVNIFA
GINRCDWVAEGVIKAIREVGVNVPLVVRLSGTKAEEGRRILADSGEAVIVADTLAEAAEKAVAAWRAAAKKKAA

Sequences:

>Translated_394_residues
MDIHEYQAKELLARHGVHVPRGGLAYSPEQATYRAREIGGGKWVLKAQVHSGARGKAGGIKLCANDEEISSAAEAMLGRK
LVTQQTGPRGKLISRLYLEEAVDIAQELYVGFVLDRKEERVMIVASAAGGMEIEDIVEKEPNSILRTSVDPGVGMQRFQA
REIAFGLGLDHNLIGKATETIFSCYQVFRDYDASMLEINPLVVTRDGNLIALDAKMSFDENALFRRPEISELRDKSQEDP
RETFASDRGLSYVGLDGNIGCIINGAGLAMATMDMIKIAGGEPANFLDIGGGASPERVAKSFRAVLGDKNVETILVNIFA
GINRCDWVAEGVIKAIREVGVNVPLVVRLSGTKAEEGRRILADSGEAVIVADTLAEAAEKAVAAWRAAAKKKAA
>Mature_394_residues
MDIHEYQAKELLARHGVHVPRGGLAYSPEQATYRAREIGGGKWVLKAQVHSGARGKAGGIKLCANDEEISSAAEAMLGRK
LVTQQTGPRGKLISRLYLEEAVDIAQELYVGFVLDRKEERVMIVASAAGGMEIEDIVEKEPNSILRTSVDPGVGMQRFQA
REIAFGLGLDHNLIGKATETIFSCYQVFRDYDASMLEINPLVVTRDGNLIALDAKMSFDENALFRRPEISELRDKSQEDP
RETFASDRGLSYVGLDGNIGCIINGAGLAMATMDMIKIAGGEPANFLDIGGGASPERVAKSFRAVLGDKNVETILVNIFA
GINRCDWVAEGVIKAIREVGVNVPLVVRLSGTKAEEGRRILADSGEAVIVADTLAEAAEKAVAAWRAAAKKKAA

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0045

COG function: function code C; Succinyl-CoA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI11321583, Length=389, Percent_Identity=41.1311053984576, Blast_Score=336, Evalue=2e-92,
Organism=Homo sapiens, GI157779135, Length=378, Percent_Identity=39.4179894179894, Blast_Score=287, Evalue=1e-77,
Organism=Homo sapiens, GI294862256, Length=363, Percent_Identity=39.6694214876033, Blast_Score=276, Evalue=2e-74,
Organism=Escherichia coli, GI1786948, Length=384, Percent_Identity=51.5625, Blast_Score=392, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17539378, Length=394, Percent_Identity=40.3553299492386, Blast_Score=314, Evalue=4e-86,
Organism=Caenorhabditis elegans, GI17567829, Length=388, Percent_Identity=40.7216494845361, Blast_Score=311, Evalue=3e-85,
Organism=Saccharomyces cerevisiae, GI6321683, Length=392, Percent_Identity=42.8571428571429, Blast_Score=332, Evalue=7e-92,
Organism=Drosophila melanogaster, GI21356231, Length=394, Percent_Identity=39.3401015228426, Blast_Score=299, Evalue=3e-81,
Organism=Drosophila melanogaster, GI161078106, Length=389, Percent_Identity=39.0745501285347, Blast_Score=286, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24645208, Length=389, Percent_Identity=39.0745501285347, Blast_Score=286, Evalue=1e-77,
Organism=Drosophila melanogaster, GI281361397, Length=390, Percent_Identity=39.2307692307692, Blast_Score=285, Evalue=3e-77,
Organism=Drosophila melanogaster, GI281361395, Length=390, Percent_Identity=39.2307692307692, Blast_Score=285, Evalue=3e-77,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2361 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): MTKA_RHILO (Q98KT9)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_102939.1
- ProteinModelPortal:   Q98KT9
- SMR:   Q98KT9
- GeneID:   1225602
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr1324
- NMPDR:   fig|266835.1.peg.1044
- HOGENOM:   HBG315432
- OMA:   GLDGDIG
- ProtClustDB:   PRK14046
- BRENDA:   6.2.1.9
- HAMAP:   MF_00558
- InterPro:   IPR013650
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR005811
- InterPro:   IPR017866
- InterPro:   IPR005809
- InterPro:   IPR016102
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.261
- PANTHER:   PTHR11815
- TIGRFAMs:   TIGR01016

Pfam domain/function: PF08442 ATP-grasp_2; PF00549 Ligase_CoA; SSF52210 CoA_ligase

EC number: =6.2.1.9

Molecular weight: Translated: 42482; Mature: 42482

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS50975 ATP_GRASP; PS01217 SUCCINYL_COA_LIG_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIHEYQAKELLARHGVHVPRGGLAYSPEQATYRAREIGGGKWVLKAQVHSGARGKAGGI
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEEECCCCCCCCCCE
KLCANDEEISSAAEAMLGRKLVTQQTGPRGKLISRLYLEEAVDIAQELYVGFVLDRKEER
EEECCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCE
VMIVASAAGGMEIEDIVEKEPNSILRTSVDPGVGMQRFQAREIAFGLGLDHNLIGKATET
EEEEEECCCCCCHHHHHHCCCCHHEECCCCCCCCHHHHHHHHHHEECCCCCCHHHHHHHH
IFSCYQVFRDYDASMLEINPLVVTRDGNLIALDAKMSFDENALFRRPEISELRDKSQEDP
HHHHHHHHHCCCCCEEEECCEEEEECCCEEEEECCCCCCCCCCCCCCCHHHHHHCCCCCH
RETFASDRGLSYVGLDGNIGCIINGAGLAMATMDMIKIAGGEPANFLDIGGGASPERVAK
HHHHHCCCCCEEEECCCCEEEEEECCCHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHH
SFRAVLGDKNVETILVNIFAGINRCDWVAEGVIKAIREVGVNVPLVVRLSGTKAEEGRRI
HHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEE
LADSGEAVIVADTLAEAAEKAVAAWRAAAKKKAA
EECCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDIHEYQAKELLARHGVHVPRGGLAYSPEQATYRAREIGGGKWVLKAQVHSGARGKAGGI
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEEECCCCCCCCCCE
KLCANDEEISSAAEAMLGRKLVTQQTGPRGKLISRLYLEEAVDIAQELYVGFVLDRKEER
EEECCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCE
VMIVASAAGGMEIEDIVEKEPNSILRTSVDPGVGMQRFQAREIAFGLGLDHNLIGKATET
EEEEEECCCCCCHHHHHHCCCCHHEECCCCCCCCHHHHHHHHHHEECCCCCCHHHHHHHH
IFSCYQVFRDYDASMLEINPLVVTRDGNLIALDAKMSFDENALFRRPEISELRDKSQEDP
HHHHHHHHHCCCCCEEEECCEEEEECCCEEEEECCCCCCCCCCCCCCCHHHHHHCCCCCH
RETFASDRGLSYVGLDGNIGCIINGAGLAMATMDMIKIAGGEPANFLDIGGGASPERVAK
HHHHHCCCCCEEEECCCCEEEEEECCCHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHH
SFRAVLGDKNVETILVNIFAGINRCDWVAEGVIKAIREVGVNVPLVVRLSGTKAEEGRRI
HHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEE
LADSGEAVIVADTLAEAAEKAVAAWRAAAKKKAA
EECCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968