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The map label for this gene is trmFO

Identifier: 13471167

GI number: 13471167

Start: 891150

End: 892568

Strand: Direct

Name: trmFO

Synonym: mlr1064

Alternate gene names: 13471167

Gene position: 891150-892568 (Clockwise)

Preceding gene: 13471166

Following gene: 13471174

Centisome position: 12.67

GC content: 65.75

Gene sequence:

>1419_bases
ATGAGCAAAAATCCCATCCACATCATCGGCGGCGGTCTCGCCGGTTCCGAAGCTGCGTGGCAGGCCGCGCAAGCCGGCGT
TCCCGTGGTGCTGCATGAAATGCGCCCGGTTCGCGGTACGGACGCGCACAAGACCGACGGCCTGGCCGAACTCGTATGTT
CCAATTCCTTCCGTTCCGATGACGCCGAGAACAACGCGGTCGGCCTGCTGCACGCCGAAATGCGGCTGGCCGGCTCGCTG
ATCATGAGCGCGGGCGACGCCAACCAGGTGCCGGCCGGCGGCGCGCTGGCCGTCGACCGCGACGGTTTCTCCGATGCCGT
GACGAAAAAGCTCGAGGCGCACCCGCTGATCAGCATCCAGCGCGAGGAAGTGTCCGGCCTGCCGCCGGCGGACTGGGACC
AGGCGATCATCGCCACGGGACCGCTGACCGCCCCATCGCTTGCCCGGTCGATCGCCGAAGCGACGGGCGCCGATGCGCTC
GCCTTCTTCGACGCCATCGCGCCGATCATCCATTTCGACACGATCGACATGGACACCTGCTGGTTCCAGTCGCGCTACGA
CAAGGTCGGCCCCGGCGGCACCGGCAAGGACTACATCAACTGTCCGATGGACAAGGATCAGTATCTTGCCTTCGTGCAGG
CGCTCATCGACGGCCAGAAGACCGAATTCAAGCAATGGGAAGGCACGCCCTATTTCGACGGCTGCCTGCCGATCGAGATC
ATGGCCGAACGCGGCGTCGAGACGCTGCGCTACGGTCCGATGAAGCCAATGGGCCTGACCAACGCGCACAATCCGATGGT
AAAAGCCTATGCCGTCGTCCAGTTGCGGCAGGACAATGCGCTGGGCACGCTCTACAACATGGTCGGCTTCCAGACCAAGC
TGAAGCACGCCGAGCAGGTCCGCGTCTTCCGCACCATTCCGGGCCTGGAAAACGCCGATTTCGCCCGCCTCGGCGGCCTG
CACCGCAACACCTACATCAATTCGCCGACCTTGCTCGACCAATCCCTGCAGCTGAAGTCGCGCCCTGGCCTGCGCTTCGC
CGGCCAGATCACCGGCTGCGAGGGCTATGTCGAAAGCGCCGCCATCGGCCTCCTCGCCGGCCGCTTCGCCGCCGCCGAGC
GGCTCGGCCAGGCGCCGTCGCTGCCGCCGCTCACCACGGCCTTCGGTGCGCTGCTCAACCACATCACCGGCGGCCACATC
GTCTCCGACGACGAACCGGGCAAGCGCTCCTTCCAGCCGATGAATGTCAATTTCGGCCTGTTCCCGCCGGTGGAAGCGGT
GAAAATCGAAGGCAAGCGCCTGCGCGGCAAGGACAAGACGGTGGCAAAACGGCACGCCATCACATCGCGCGCGCTGGGCG
ATTGCCGGCAATGGCTGGGCCTGCCGGCGCCAACAGAAACGGCCGAAGCGGCGGAGTAA

Upstream 100 bases:

>100_bases
CGGGTGTTGTTGTTTTCAGAGCAGGCTTTTCGATCAAGCGTTTCCCGTTACCGCCGCACGGCAATTGCAATCGCCGCCCC
AAGCGATTATTCCGGCGCTC

Downstream 100 bases:

>100_bases
TTCCCCTATTCCGCCGGCTCGACATTCGCCGTCGGCAGTCCCGGCTTGCCAGCCTCACCCGGATTTCGCCTGACATAGGC
AGCTTTCAGGCTCTCCGAGG

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MSKNPIHIIGGGLAGSEAAWQAAQAGVPVVLHEMRPVRGTDAHKTDGLAELVCSNSFRSDDAENNAVGLLHAEMRLAGSL
IMSAGDANQVPAGGALAVDRDGFSDAVTKKLEAHPLISIQREEVSGLPPADWDQAIIATGPLTAPSLARSIAEATGADAL
AFFDAIAPIIHFDTIDMDTCWFQSRYDKVGPGGTGKDYINCPMDKDQYLAFVQALIDGQKTEFKQWEGTPYFDGCLPIEI
MAERGVETLRYGPMKPMGLTNAHNPMVKAYAVVQLRQDNALGTLYNMVGFQTKLKHAEQVRVFRTIPGLENADFARLGGL
HRNTYINSPTLLDQSLQLKSRPGLRFAGQITGCEGYVESAAIGLLAGRFAAAERLGQAPSLPPLTTAFGALLNHITGGHI
VSDDEPGKRSFQPMNVNFGLFPPVEAVKIEGKRLRGKDKTVAKRHAITSRALGDCRQWLGLPAPTETAEAAE

Sequences:

>Translated_472_residues
MSKNPIHIIGGGLAGSEAAWQAAQAGVPVVLHEMRPVRGTDAHKTDGLAELVCSNSFRSDDAENNAVGLLHAEMRLAGSL
IMSAGDANQVPAGGALAVDRDGFSDAVTKKLEAHPLISIQREEVSGLPPADWDQAIIATGPLTAPSLARSIAEATGADAL
AFFDAIAPIIHFDTIDMDTCWFQSRYDKVGPGGTGKDYINCPMDKDQYLAFVQALIDGQKTEFKQWEGTPYFDGCLPIEI
MAERGVETLRYGPMKPMGLTNAHNPMVKAYAVVQLRQDNALGTLYNMVGFQTKLKHAEQVRVFRTIPGLENADFARLGGL
HRNTYINSPTLLDQSLQLKSRPGLRFAGQITGCEGYVESAAIGLLAGRFAAAERLGQAPSLPPLTTAFGALLNHITGGHI
VSDDEPGKRSFQPMNVNFGLFPPVEAVKIEGKRLRGKDKTVAKRHAITSRALGDCRQWLGLPAPTETAEAAE
>Mature_471_residues
SKNPIHIIGGGLAGSEAAWQAAQAGVPVVLHEMRPVRGTDAHKTDGLAELVCSNSFRSDDAENNAVGLLHAEMRLAGSLI
MSAGDANQVPAGGALAVDRDGFSDAVTKKLEAHPLISIQREEVSGLPPADWDQAIIATGPLTAPSLARSIAEATGADALA
FFDAIAPIIHFDTIDMDTCWFQSRYDKVGPGGTGKDYINCPMDKDQYLAFVQALIDGQKTEFKQWEGTPYFDGCLPIEIM
AERGVETLRYGPMKPMGLTNAHNPMVKAYAVVQLRQDNALGTLYNMVGFQTKLKHAEQVRVFRTIPGLENADFARLGGLH
RNTYINSPTLLDQSLQLKSRPGLRFAGQITGCEGYVESAAIGLLAGRFAAAERLGQAPSLPPLTTAFGALLNHITGGHIV
SDDEPGKRSFQPMNVNFGLFPPVEAVKIEGKRLRGKDKTVAKRHAITSRALGDCRQWLGLPAPTETAEAAE

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMFO_RHILO (Q98LE1)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_102736.1
- ProteinModelPortal:   Q98LE1
- GeneID:   1225399
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr1064
- NMPDR:   fig|266835.1.peg.841
- HOGENOM:   HBG285041
- OMA:   FEGCLPI
- ProtClustDB:   PRK05335
- GO:   GO:0005737
- HAMAP:   MF_01037
- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00137

Pfam domain/function: PF01134 GIDA

EC number: =2.1.1.74

Molecular weight: Translated: 50648; Mature: 50517

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKNPIHIIGGGLAGSEAAWQAAQAGVPVVLHEMRPVRGTDAHKTDGLAELVCSNSFRSD
CCCCCEEEEECCCCCCHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHCCCCCCC
DAENNAVGLLHAEMRLAGSLIMSAGDANQVPAGGALAVDRDGFSDAVTKKLEAHPLISIQ
CCCCCEEEEEEHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCCCEEEE
REEVSGLPPADWDQAIIATGPLTAPSLARSIAEATGADALAFFDAIAPIIHFDTIDMDTC
HHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHEECCCCCHHH
WFQSRYDKVGPGGTGKDYINCPMDKDQYLAFVQALIDGQKTEFKQWEGTPYFDGCLPIEI
HHHHHCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCHHH
MAERGVETLRYGPMKPMGLTNAHNPMVKAYAVVQLRQDNALGTLYNMVGFQTKLKHAEQV
HHHCCCHHHHCCCCCCCCCCCCCCCHHEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
RVFRTIPGLENADFARLGGLHRNTYINSPTLLDQSLQLKSRPGLRFAGQITGCEGYVESA
HHHHHCCCCCCCCHHHHCCCCCCCCCCCCHHHCCHHHHCCCCCCEEEEEECCCHHHHHHH
AIGLLAGRFAAAERLGQAPSLPPLTTAFGALLNHITGGHIVSDDEPGKRSFQPMNVNFGL
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCC
FPPVEAVKIEGKRLRGKDKTVAKRHAITSRALGDCRQWLGLPAPTETAEAAE
CCCCCEEEECCHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SKNPIHIIGGGLAGSEAAWQAAQAGVPVVLHEMRPVRGTDAHKTDGLAELVCSNSFRSD
CCCCEEEEECCCCCCHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHCCCCCCC
DAENNAVGLLHAEMRLAGSLIMSAGDANQVPAGGALAVDRDGFSDAVTKKLEAHPLISIQ
CCCCCEEEEEEHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCCCEEEE
REEVSGLPPADWDQAIIATGPLTAPSLARSIAEATGADALAFFDAIAPIIHFDTIDMDTC
HHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHEECCCCCHHH
WFQSRYDKVGPGGTGKDYINCPMDKDQYLAFVQALIDGQKTEFKQWEGTPYFDGCLPIEI
HHHHHCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCHHH
MAERGVETLRYGPMKPMGLTNAHNPMVKAYAVVQLRQDNALGTLYNMVGFQTKLKHAEQV
HHHCCCHHHHCCCCCCCCCCCCCCCHHEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
RVFRTIPGLENADFARLGGLHRNTYINSPTLLDQSLQLKSRPGLRFAGQITGCEGYVESA
HHHHHCCCCCCCCHHHHCCCCCCCCCCCCHHHCCHHHHCCCCCCEEEEEECCCHHHHHHH
AIGLLAGRFAAAERLGQAPSLPPLTTAFGALLNHITGGHIVSDDEPGKRSFQPMNVNFGL
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCC
FPPVEAVKIEGKRLRGKDKTVAKRHAITSRALGDCRQWLGLPAPTETAEAAE
CCCCCEEEECCHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968