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The map label for this gene is kinE [H]

Identifier: 13471092

GI number: 13471092

Start: 798102

End: 799013

Strand: Reverse

Name: kinE [H]

Synonym: mll0976

Alternate gene names: 13471092

Gene position: 799013-798102 (Counterclockwise)

Preceding gene: 13471099

Following gene: 13471091

Centisome position: 11.36

GC content: 59.1

Gene sequence:

>912_bases
GTGATTGGAGAACCAGCGAAATCGGGGAAAGACGTATACCCCCGTATGGCTGGGCCTCGCCGCAGATTCAATGGACCGCA
AAAGACCCGGAAACTAGTTTCGCCGGACTACCACCCTTTAGCTGTAATGGGGTCTCCAATGCCGCACCCGGAAGTGATGT
CCAGAAAAGCCGCCGGGATCCGTCTTGGGCGTAAAGCCGACACCGCGTCCTATCTCGACATATCGGCGGAACCGACAGTC
GGAGCCGTCCACGATTTGGGCAATCTTCTGCAGGTCGCTTCGTCCGCATTGAACCTGCTCGCACGAGATCCGACTGTCTC
CGCCGCGCCAGCCCTTGAGCCTCTCATTGTCGGCGCCAGGACGGCGTTGCAACGAGCAGACACTTTGGTCCGCGATACGA
TCATCAGAGCGCGCAAAAGCAGTCGCGAAACTGAACACGCAGATATCAACGCCTGCCTAGAAGTAATTGAGAAACTCACC
GGCTGGGCAGGAGACGCAAACATTCGCCTTGAGGTTGCCTCAGCGACCGACCTGCCGATGGTGAGGTGCGATCCCCTCGG
TCTTCAGAACGCCGTCTTGAACCTCGTTTTCAATGCACGTGACGCAATGCCTAACGGCGGGGTGATCTCAATTAGCGTTG
CGGAAGTGGTCGTCGGCCCTGCCCATCAGATCGAAGCGCGCGTGAAAGACAACGGCGTTGGAATGTCGCCCGAGACCGTG
GTCCGCGCTTTCGAACCGTTCTTCACCACCAAGGGCAATGGCCTGGGCGGCGTCGGCCTGCCGATGGTGAAGCGGTTCGT
CGAGGAGCATGGCGGAACCATCGAAGTCGAGAGCTCGTTCGGGTCTGGCACTACGGTGATATTGCGGCTCCCTGCCGAAG
ACCTTTGCTGTCCGGAAAAATCGGCGCGCTGA

Upstream 100 bases:

>100_bases
TGCCTTATAGCATCGGCGTTGGAGGCTCTGTCCGATGAGTCCGCGCGATTCCCCCTAAAGCGCGTCACAGATTGGGTTTG
GCAGCTGGCCGACCACCAAC

Downstream 100 bases:

>100_bases
CGCATGAGCGACGAGTACAATCTGCATAGGTTCGTCCGCGCCCAAGCGCCGGTTTATGAGGCGACCATCGATGGCCTGCG
TCGCGGGACGATGAATACTT

Product: sensor/response regulator hybrid

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MIGEPAKSGKDVYPRMAGPRRRFNGPQKTRKLVSPDYHPLAVMGSPMPHPEVMSRKAAGIRLGRKADTASYLDISAEPTV
GAVHDLGNLLQVASSALNLLARDPTVSAAPALEPLIVGARTALQRADTLVRDTIIRARKSSRETEHADINACLEVIEKLT
GWAGDANIRLEVASATDLPMVRCDPLGLQNAVLNLVFNARDAMPNGGVISISVAEVVVGPAHQIEARVKDNGVGMSPETV
VRAFEPFFTTKGNGLGGVGLPMVKRFVEEHGGTIEVESSFGSGTTVILRLPAEDLCCPEKSAR

Sequences:

>Translated_303_residues
MIGEPAKSGKDVYPRMAGPRRRFNGPQKTRKLVSPDYHPLAVMGSPMPHPEVMSRKAAGIRLGRKADTASYLDISAEPTV
GAVHDLGNLLQVASSALNLLARDPTVSAAPALEPLIVGARTALQRADTLVRDTIIRARKSSRETEHADINACLEVIEKLT
GWAGDANIRLEVASATDLPMVRCDPLGLQNAVLNLVFNARDAMPNGGVISISVAEVVVGPAHQIEARVKDNGVGMSPETV
VRAFEPFFTTKGNGLGGVGLPMVKRFVEEHGGTIEVESSFGSGTTVILRLPAEDLCCPEKSAR
>Mature_303_residues
MIGEPAKSGKDVYPRMAGPRRRFNGPQKTRKLVSPDYHPLAVMGSPMPHPEVMSRKAAGIRLGRKADTASYLDISAEPTV
GAVHDLGNLLQVASSALNLLARDPTVSAAPALEPLIVGARTALQRADTLVRDTIIRARKSSRETEHADINACLEVIEKLT
GWAGDANIRLEVASATDLPMVRCDPLGLQNAVLNLVFNARDAMPNGGVISISVAEVVVGPAHQIEARVKDNGVGMSPETV
VRAFEPFFTTKGNGLGGVGLPMVKRFVEEHGGTIEVESSFGSGTTVILRLPAEDLCCPEKSAR

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=146, Percent_Identity=37.6712328767123, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788549, Length=252, Percent_Identity=24.6031746031746, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1786600, Length=219, Percent_Identity=27.3972602739726, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 32226; Mature: 32226

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGEPAKSGKDVYPRMAGPRRRFNGPQKTRKLVSPDYHPLAVMGSPMPHPEVMSRKAAGI
CCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHCE
RLGRKADTASYLDISAEPTVGAVHDLGNLLQVASSALNLLARDPTVSAAPALEPLIVGAR
ECCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
TALQRADTLVRDTIIRARKSSRETEHADINACLEVIEKLTGWAGDANIRLEVASATDLPM
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCE
VRCDPLGLQNAVLNLVFNARDAMPNGGVISISVAEVVVGPAHQIEARVKDNGVGMSPETV
EEECCCCHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHCCHHHHHEEECCCCCCCCHHHH
VRAFEPFFTTKGNGLGGVGLPMVKRFVEEHGGTIEVESSFGSGTTVILRLPAEDLCCPEK
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCEEEEECCHHHCCCCCC
SAR
CCC
>Mature Secondary Structure
MIGEPAKSGKDVYPRMAGPRRRFNGPQKTRKLVSPDYHPLAVMGSPMPHPEVMSRKAAGI
CCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHCE
RLGRKADTASYLDISAEPTVGAVHDLGNLLQVASSALNLLARDPTVSAAPALEPLIVGAR
ECCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
TALQRADTLVRDTIIRARKSSRETEHADINACLEVIEKLTGWAGDANIRLEVASATDLPM
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCE
VRCDPLGLQNAVLNLVFNARDAMPNGGVISISVAEVVVGPAHQIEARVKDNGVGMSPETV
EEECCCCHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHCCHHHHHEEECCCCCCCCHHHH
VRAFEPFFTTKGNGLGGVGLPMVKRFVEEHGGTIEVESSFGSGTTVILRLPAEDLCCPEK
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCEEEEECCHHHCCCCCC
SAR
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]