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The map label for this gene is phbC [H]

Identifier: 13471063

GI number: 13471063

Start: 755035

End: 756870

Strand: Direct

Name: phbC [H]

Synonym: mlr0937

Alternate gene names: 13471063

Gene position: 755035-756870 (Clockwise)

Preceding gene: 13471055

Following gene: 13471064

Centisome position: 10.73

GC content: 62.04

Gene sequence:

>1836_bases
ATGTCCAAAACACCCGATTCGGGCAAAACGGAAGATGACGAGCCTTCGACCGTCGAGCAATATCTGGTGAAGGATCCGGA
GCGCTTCGCGTTGAACATGGCGCGCATGATCGAGCAGGCCGGCAAAGCGGCGTCCGCCTGGGCCGAACCGCGCGAGCGCG
GCGAAGTGCGCGACCACGTCGCCGAGCCGGTCGTCGACATGGTCAAGACATTTTCCAAACTCAGCGAATACTGGCTCGCC
GACCCGCAGCGCGCGCTCGAAGCGCAGACGCGGCTGTTCGCCGGCTATATGACGGTGTGGGCGAACGCCATCCAGCGCGT
CAGCCCCAACGCCGAAGGCACCGACGACGCCGTCAAGCCGGAGCGCGGCGACAAGCGTTTCCAGGACCCGGAATGGGGCC
GCAACGCCTTCTTCGATTTCCTCAAGCAGGCCTATCTCGTCACCTCGCGCTGGGCGTCCGAACTGGTCGAGCACGCCGAA
GGTCTGGACGAGCACACCCGCCACAAGGCGAGCTTCTACGTCAAGCAGGTGTCCAATGCCATCTCGCCGTCAAATTTCAT
CCTGACCAACCCGGAGCTGTTCCGCGAGACCGTCGCTTCCAACGGCGAGAACCTGGTGCGCGGGATGAAGATGCTGGCCG
AGGACATCGCCGCCGGCAAGGGTGACCTGAAACTGCGCCAGGCCGACTATTCGCCCTTCGAGATCGGCAGGAACATCGCC
ACCACACCCGGCAAGGTGGTCGGCCGCAGCGATGTCGCCGAGATCATCCAGTATGATCCCGCGACCGAGACGGTGCTGAA
ACGCCCGCTGCTGATCTGCCCGCCCTGGATCAACAAGTTCTACATACTCGACCTCAACCCGCAGAAATCCTTCATCCGCT
GGGCGATCGAGCAAGGCCACACCGTTTTCGTCATCTCCTGGATCAATCCGGACGAGCGCCACGGCGCCAAGAATTGGGAA
GCCTATATCAGGGAAGGCCTGCAATACGGCCTCGACACGATCGAGAAGGCCACCGGCGAGCGCGACGTAAACGCGATCGG
TTACTGCGTTGGCGGCACGCTGCTGGCGGCGGCCCTGGCGCTGATGGCCAGGGAAGGCGACGACCGCATCAAGTCGGCGA
CCTTTTTCACCACGCAAGTCGACTTCACCTATGCCGGCGACCTGAAAGTCTTCGTCGACGAGGAACAGGTCGCAGCTGTG
GAAAAGTCGATGAACGAAAAGGGCTATCTCGACGGCACCAAGATGGCGACCGCCTTCAACATGCTGCGCTCCGGCGACCT
GATCTGGCCCTATGTCGTCAACAACTACATGCGCGGCAAGGATCCCCTGCCCTTCGACCTGCTCTACTGGAATGCCGATT
CGACCCGAATGGCGGCGGCCAACCATTCCTTCTATCTGCGCAATTGCTATCTCGAGAACAACCTCTCGCGTGGCACGATG
GAGCTGGCGGGGCATACGGTCTCGCTTGGGGATGTCACCATTCCGGTCTACAACCTCGCCTCGCGGGAAGATCACATCGC
GCCGGCACTGTCCGTCTTCCTCGGCTCGAAATATTTCGGCGGCAAGGTCGACTACGTGATGGCGGGATCGGGCCACATAG
CCGGTGTCGTCAATCCGCCCGCCTCCGGCAAGTACCAGTATTGGACCGGCGGCAAGCCGGAAGGCGATTTCGGCCAATGG
ATTGCCAAGGCCGCTGAGCATCCCGGGTCGTGGTGGCCGCACTGGCAAAGCTGGATCGAGGCCAAGGACGACACCCGCGT
GCCTGCCCGCAAACCCGGCAAGCATATGAAAACCTTGGGCGATGCTCCCGGCAGCTATGTGAAGGTGCGTGTGTAA

Upstream 100 bases:

>100_bases
ATATTGTTTCCTTGGCAGCCGGGGTAGAGCGAGATTGGACCCTGTCGGATGTCCCATGTTAATATGTCAAGAAAACGCTT
CGGGAGGAACCCCGCGAACC

Downstream 100 bases:

>100_bases
CGGACTGTAATGTCTGGTGAGTTGACGGCCCGCCAAAAGCGGGCGAAATGGTGTCGTCAACCATCAAGCAGCCGTAATTC
GGCAATCGCTGCAAAAATAC

Product: poly-beta-hydroxybutyrate synthase

Products: NA

Alternate protein names: PHA synthase; PHB synthase; Poly(3-hydroxyalkanoate) polymerase; PHA polymerase; Poly(3-hydroxybutyrate) polymerase; PHB polymerase; Polyhydroxyalkanoic acid synthase [H]

Number of amino acids: Translated: 611; Mature: 610

Protein sequence:

>611_residues
MSKTPDSGKTEDDEPSTVEQYLVKDPERFALNMARMIEQAGKAASAWAEPRERGEVRDHVAEPVVDMVKTFSKLSEYWLA
DPQRALEAQTRLFAGYMTVWANAIQRVSPNAEGTDDAVKPERGDKRFQDPEWGRNAFFDFLKQAYLVTSRWASELVEHAE
GLDEHTRHKASFYVKQVSNAISPSNFILTNPELFRETVASNGENLVRGMKMLAEDIAAGKGDLKLRQADYSPFEIGRNIA
TTPGKVVGRSDVAEIIQYDPATETVLKRPLLICPPWINKFYILDLNPQKSFIRWAIEQGHTVFVISWINPDERHGAKNWE
AYIREGLQYGLDTIEKATGERDVNAIGYCVGGTLLAAALALMAREGDDRIKSATFFTTQVDFTYAGDLKVFVDEEQVAAV
EKSMNEKGYLDGTKMATAFNMLRSGDLIWPYVVNNYMRGKDPLPFDLLYWNADSTRMAAANHSFYLRNCYLENNLSRGTM
ELAGHTVSLGDVTIPVYNLASREDHIAPALSVFLGSKYFGGKVDYVMAGSGHIAGVVNPPASGKYQYWTGGKPEGDFGQW
IAKAAEHPGSWWPHWQSWIEAKDDTRVPARKPGKHMKTLGDAPGSYVKVRV

Sequences:

>Translated_611_residues
MSKTPDSGKTEDDEPSTVEQYLVKDPERFALNMARMIEQAGKAASAWAEPRERGEVRDHVAEPVVDMVKTFSKLSEYWLA
DPQRALEAQTRLFAGYMTVWANAIQRVSPNAEGTDDAVKPERGDKRFQDPEWGRNAFFDFLKQAYLVTSRWASELVEHAE
GLDEHTRHKASFYVKQVSNAISPSNFILTNPELFRETVASNGENLVRGMKMLAEDIAAGKGDLKLRQADYSPFEIGRNIA
TTPGKVVGRSDVAEIIQYDPATETVLKRPLLICPPWINKFYILDLNPQKSFIRWAIEQGHTVFVISWINPDERHGAKNWE
AYIREGLQYGLDTIEKATGERDVNAIGYCVGGTLLAAALALMAREGDDRIKSATFFTTQVDFTYAGDLKVFVDEEQVAAV
EKSMNEKGYLDGTKMATAFNMLRSGDLIWPYVVNNYMRGKDPLPFDLLYWNADSTRMAAANHSFYLRNCYLENNLSRGTM
ELAGHTVSLGDVTIPVYNLASREDHIAPALSVFLGSKYFGGKVDYVMAGSGHIAGVVNPPASGKYQYWTGGKPEGDFGQW
IAKAAEHPGSWWPHWQSWIEAKDDTRVPARKPGKHMKTLGDAPGSYVKVRV
>Mature_610_residues
SKTPDSGKTEDDEPSTVEQYLVKDPERFALNMARMIEQAGKAASAWAEPRERGEVRDHVAEPVVDMVKTFSKLSEYWLAD
PQRALEAQTRLFAGYMTVWANAIQRVSPNAEGTDDAVKPERGDKRFQDPEWGRNAFFDFLKQAYLVTSRWASELVEHAEG
LDEHTRHKASFYVKQVSNAISPSNFILTNPELFRETVASNGENLVRGMKMLAEDIAAGKGDLKLRQADYSPFEIGRNIAT
TPGKVVGRSDVAEIIQYDPATETVLKRPLLICPPWINKFYILDLNPQKSFIRWAIEQGHTVFVISWINPDERHGAKNWEA
YIREGLQYGLDTIEKATGERDVNAIGYCVGGTLLAAALALMAREGDDRIKSATFFTTQVDFTYAGDLKVFVDEEQVAAVE
KSMNEKGYLDGTKMATAFNMLRSGDLIWPYVVNNYMRGKDPLPFDLLYWNADSTRMAAANHSFYLRNCYLENNLSRGTME
LAGHTVSLGDVTIPVYNLASREDHIAPALSVFLGSKYFGGKVDYVMAGSGHIAGVVNPPASGKYQYWTGGKPEGDFGQWI
AKAAEHPGSWWPHWQSWIEAKDDTRVPARKPGKHMKTLGDAPGSYVKVRV

Specific function: Polymerizes d(-)-3-hydroxybutyryl-CoA to create PHB which consists of thousands of hydroxybutyrate molecules linked end to end. PHB serves as an intracellular energy reserve material when cells grow under conditions of nutrient limitation [H]

COG id: COG3243

COG function: function code I; Poly(3-hydroxyalkanoate) synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PHA/PHB synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010963
- InterPro:   IPR010941 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF07167 PhaC_N [H]

EC number: NA

Molecular weight: Translated: 68380; Mature: 68249

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTPDSGKTEDDEPSTVEQYLVKDPERFALNMARMIEQAGKAASAWAEPRERGEVRDHV
CCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHH
AEPVVDMVKTFSKLSEYWLADPQRALEAQTRLFAGYMTVWANAIQRVSPNAEGTDDAVKP
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
ERGDKRFQDPEWGRNAFFDFLKQAYLVTSRWASELVEHAEGLDEHTRHKASFYVKQVSNA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHC
ISPSNFILTNPELFRETVASNGENLVRGMKMLAEDIAAGKGDLKLRQADYSPFEIGRNIA
CCCCCEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHCCCCC
TTPGKVVGRSDVAEIIQYDPATETVLKRPLLICPPWINKFYILDLNPQKSFIRWAIEQGH
CCCCCCCCHHHHHHHHHCCCCHHHHHHCCCEEECCCCCEEEEEEECCHHHHHHHHHHCCC
TVFVISWINPDERHGAKNWEAYIREGLQYGLDTIEKATGERDVNAIGYCVGGTLLAAALA
EEEEEEECCCCHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
LMAREGDDRIKSATFFTTQVDFTYAGDLKVFVDEEQVAAVEKSMNEKGYLDGTKMATAFN
HHHHCCCHHHHHEEEEEEEEEEEEECCEEEEECHHHHHHHHHHCCCCCCCCHHHHHHHHH
MLRSGDLIWPYVVNNYMRGKDPLPFDLLYWNADSTRMAAANHSFYLRNCYLENNLSRGTM
HHHCCCEEEHHHHCCHHCCCCCCCEEEEEECCCCCEEEECCCCEEEEEEEECCCCCCCCH
ELAGHTVSLGDVTIPVYNLASREDHIAPALSVFLGSKYFGGKVDYVMAGSGHIAGVVNPP
HHCCCEEEECCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEECCC
ASGKYQYWTGGKPEGDFGQWIAKAAEHPGSWWPHWQSWIEAKDDTRVPARKPGKHMKTLG
CCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHC
DAPGSYVKVRV
CCCCCEEEEEC
>Mature Secondary Structure 
SKTPDSGKTEDDEPSTVEQYLVKDPERFALNMARMIEQAGKAASAWAEPRERGEVRDHV
CCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHH
AEPVVDMVKTFSKLSEYWLADPQRALEAQTRLFAGYMTVWANAIQRVSPNAEGTDDAVKP
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
ERGDKRFQDPEWGRNAFFDFLKQAYLVTSRWASELVEHAEGLDEHTRHKASFYVKQVSNA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHC
ISPSNFILTNPELFRETVASNGENLVRGMKMLAEDIAAGKGDLKLRQADYSPFEIGRNIA
CCCCCEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHCCCCC
TTPGKVVGRSDVAEIIQYDPATETVLKRPLLICPPWINKFYILDLNPQKSFIRWAIEQGH
CCCCCCCCHHHHHHHHHCCCCHHHHHHCCCEEECCCCCEEEEEEECCHHHHHHHHHHCCC
TVFVISWINPDERHGAKNWEAYIREGLQYGLDTIEKATGERDVNAIGYCVGGTLLAAALA
EEEEEEECCCCHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
LMAREGDDRIKSATFFTTQVDFTYAGDLKVFVDEEQVAAVEKSMNEKGYLDGTKMATAFN
HHHHCCCHHHHHEEEEEEEEEEEEECCEEEEECHHHHHHHHHHCCCCCCCCHHHHHHHHH
MLRSGDLIWPYVVNNYMRGKDPLPFDLLYWNADSTRMAAANHSFYLRNCYLENNLSRGTM
HHHCCCEEEHHHHCCHHCCCCCCCEEEEEECCCCCEEEECCCCEEEEEEEECCCCCCCCH
ELAGHTVSLGDVTIPVYNLASREDHIAPALSVFLGSKYFGGKVDYVMAGSGHIAGVVNPP
HHCCCEEEECCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEECCC
ASGKYQYWTGGKPEGDFGQWIAKAAEHPGSWWPHWQSWIEAKDDTRVPARKPGKHMKTLG
CCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHC
DAPGSYVKVRV
CCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7582015; 9734305; 11481430 [H]