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The map label for this gene is folE

Identifier: 13471052

GI number: 13471052

Start: 744830

End: 745423

Strand: Direct

Name: folE

Synonym: mlr0922

Alternate gene names: 13471052

Gene position: 744830-745423 (Clockwise)

Preceding gene: 13471050

Following gene: 13471053

Centisome position: 10.59

GC content: 63.8

Gene sequence:

>594_bases
ATGGAAAAGCCGGTCACCGACCGACCGAGCGAAGCCGAGGTCGAGGCGGCCGTGCGCACATTGCTGCGCTGGACCGGCGA
CAATCCGGACCGCGAGGGGCTGATCGATACGCCCAAGCGCGTGGCCAAGGCCTTCCGCGAAATGTTCGGCGGCTACGACA
TGTGCCCGGCCGAGGAGCTCGGCCGCACCTTCGAGGAGGTCGCCGGCTACGACGATCTCGTCATAGTGAAGGATATTCAC
TTCCATTCGCATTGCGAGCACCACATGGTGCCGATCATCGGCAAGGCGCATGTCGGCTATCTGCCGGATGGCAAGGTCGT
CGGCCTGTCGAAGATCGCGCGCGTCGTCGATATCTTCGCCCATCGCCTGCAGACGCAGGAAGCACTGACCGCGCAAATCG
CCGGCGTCATCCAGGATGTGCTCAATCCGCGCGGCGTCGCCGTCATGATTGAGGCCGAGCACATGTGCATGGCCATGCGC
GGCATCCGCAAGCAGGGCTCCACCACCCTCACCTCCACCTTCACCGGCGTTTTCAAGGATACGCCCGAGGAGCAGGTTCG
TTTCGTCACCATGGTTCGCGGCGGCGGGGCATAA

Upstream 100 bases:

>100_bases
AGGTCGTGGTCCGTTCAACTCGTTCCTCCTTGGAGAGGAACTACGGGAGACGCCTTTTATGGATGCCGTCATAAAGAAAC
TCATGCCCCAGTCCGCCTAC

Downstream 100 bases:

>100_bases
CGTCTAGGCCGATATCGCTAGGCCATGATCCCGAAAAACGCGGACCGGTTTTCTCTGGACAAACGAAAACGTTTGTCCAG
AGATCATGGTCAAATAGGAA

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MEKPVTDRPSEAEVEAAVRTLLRWTGDNPDREGLIDTPKRVAKAFREMFGGYDMCPAEELGRTFEEVAGYDDLVIVKDIH
FHSHCEHHMVPIIGKAHVGYLPDGKVVGLSKIARVVDIFAHRLQTQEALTAQIAGVIQDVLNPRGVAVMIEAEHMCMAMR
GIRKQGSTTLTSTFTGVFKDTPEEQVRFVTMVRGGGA

Sequences:

>Translated_197_residues
MEKPVTDRPSEAEVEAAVRTLLRWTGDNPDREGLIDTPKRVAKAFREMFGGYDMCPAEELGRTFEEVAGYDDLVIVKDIH
FHSHCEHHMVPIIGKAHVGYLPDGKVVGLSKIARVVDIFAHRLQTQEALTAQIAGVIQDVLNPRGVAVMIEAEHMCMAMR
GIRKQGSTTLTSTFTGVFKDTPEEQVRFVTMVRGGGA
>Mature_197_residues
MEKPVTDRPSEAEVEAAVRTLLRWTGDNPDREGLIDTPKRVAKAFREMFGGYDMCPAEELGRTFEEVAGYDDLVIVKDIH
FHSHCEHHMVPIIGKAHVGYLPDGKVVGLSKIARVVDIFAHRLQTQEALTAQIAGVIQDVLNPRGVAVMIEAEHMCMAMR
GIRKQGSTTLTSTFTGVFKDTPEEQVRFVTMVRGGGA

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family

Homologues:

Organism=Homo sapiens, GI66932968, Length=197, Percent_Identity=45.6852791878173, Blast_Score=180, Evalue=7e-46,
Organism=Homo sapiens, GI4503949, Length=197, Percent_Identity=45.6852791878173, Blast_Score=180, Evalue=7e-46,
Organism=Homo sapiens, GI66932970, Length=184, Percent_Identity=41.304347826087, Blast_Score=134, Evalue=5e-32,
Organism=Homo sapiens, GI66932972, Length=155, Percent_Identity=45.1612903225806, Blast_Score=132, Evalue=1e-31,
Organism=Escherichia coli, GI1788476, Length=163, Percent_Identity=42.3312883435583, Blast_Score=117, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17560486, Length=177, Percent_Identity=46.8926553672316, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6321706, Length=183, Percent_Identity=47.5409836065574, Blast_Score=171, Evalue=5e-44,
Organism=Drosophila melanogaster, GI24656782, Length=193, Percent_Identity=47.6683937823834, Blast_Score=170, Evalue=6e-43,
Organism=Drosophila melanogaster, GI24656796, Length=193, Percent_Identity=47.6683937823834, Blast_Score=169, Evalue=9e-43,
Organism=Drosophila melanogaster, GI24656791, Length=193, Percent_Identity=47.6683937823834, Blast_Score=169, Evalue=1e-42,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): GCH1_RHILO (Q98LQ6)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_102621.1
- ProteinModelPortal:   Q98LQ6
- SMR:   Q98LQ6
- GeneID:   1225284
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr0922
- NMPDR:   fig|266835.1.peg.726
- HOGENOM:   HBG370191
- OMA:   LTTTFTG
- ProtClustDB:   PRK09347
- BRENDA:   3.5.4.16
- GO:   GO:0005737
- HAMAP:   MF_00223
- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234
- TIGRFAMs:   TIGR00063

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =3.5.4.16

Molecular weight: Translated: 21774; Mature: 21774

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKPVTDRPSEAEVEAAVRTLLRWTGDNPDREGLIDTPKRVAKAFREMFGGYDMCPAEEL
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHH
GRTFEEVAGYDDLVIVKDIHFHSHCEHHMVPIIGKAHVGYLPDGKVVGLSKIARVVDIFA
HHHHHHHCCCCCEEEEEECCHHHCCCCCCCEEECCCCCCCCCCCCEECHHHHHHHHHHHH
HRLQTQEALTAQIAGVIQDVLNPRGVAVMIEAEHMCMAMRGIRKQGSTTLTSTFTGVFKD
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
TPEEQVRFVTMVRGGGA
CHHHHHEEHEEECCCCC
>Mature Secondary Structure
MEKPVTDRPSEAEVEAAVRTLLRWTGDNPDREGLIDTPKRVAKAFREMFGGYDMCPAEEL
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHH
GRTFEEVAGYDDLVIVKDIHFHSHCEHHMVPIIGKAHVGYLPDGKVVGLSKIARVVDIFA
HHHHHHHCCCCCEEEEEECCHHHCCCCCCCEEECCCCCCCCCCCCEECHHHHHHHHHHHH
HRLQTQEALTAQIAGVIQDVLNPRGVAVMIEAEHMCMAMRGIRKQGSTTLTSTFTGVFKD
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
TPEEQVRFVTMVRGGGA
CHHHHHEEHEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968