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The map label for this gene is dme [H]

Identifier: 13470964

GI number: 13470964

Start: 638569

End: 640893

Strand: Direct

Name: dme [H]

Synonym: mlr0809

Alternate gene names: 13470964

Gene position: 638569-640893 (Clockwise)

Preceding gene: 13470963

Following gene: 13470965

Centisome position: 9.08

GC content: 64.86

Gene sequence:

>2325_bases
ATGGAAGGCGAGAACAAGAAAACGGCACGTTCGGACCTCGACGAAGCCGCCCTCTACTTCCACAAGCACCCGACGCCGGG
AAAGCTCGAGATCCAGGCAACCAAGCCGCTCGGCAACCAGCGTGACCTGGCGCTCGCCTATTCGCCTGGCGTGGCTGCGC
CTTGTCTTGAGATCCGCGACAATCCGGCAACCGCTGCCGACTACACGGCGCGCGCCAACCTCGTCGGCGTCGTCTCCAAC
GGCTCGGCCGTGCTCGGCCTCGGCAACATCGGCCCGCTGGCCTCCAAGCCGGTCATGGAAGGCAAAGCGGTTCTGTTCAA
GAAGTTCGCCGGCATCGACGTCTTCGACATCGAGATCGACGCTCCCGAGATCGAGCGCATGGTGGAGACCGTCGCCGCCC
TCGAACCCACTTTCGGCGGCATCAATCTCGAGGACATCAAGGCGCCGGAGTGTTTTGAAGTCGAGGAACGGCTGAAAGCC
CGCATGAGCATACCGGTATTCCACGACGACCAGCATGGCACCGCCATCATCGTCGCCGCCGCCGTGCTCAACGGACTGGA
ATTCGCCGGCAAAACGATCTCGGACATCAAGGTCGTCACCTCCGGCGCCGGTGCGGCCGCCCTTGCCTGCCTGAACCTTC
TGGTGTCGCTCGGCGTGCGCATCGAAAACATCTGGGTCACCGACCGTTTCGGCGTCGCCTACAAGGGCCGCACCGAAGAG
ATGGACCGCTGGAAAGACCCCTATGTGAAGGACACCGAGGCGCGCACGCTGGCCGATGTCATCCCGGGCGCCGATGTCTT
CCTCGGCCTCTCTGCGGCGGGCGTGCTGAAGCCGGAACTGCTGCAGCATATGGCGCCAAAGCCGCTGATCCTGGCGCTCG
CCAATCCCAATCCCGAGATCATGCCGGAAGTGGCGCGCGCGGCGCGTCCCGATGCCATGATCTGCACCGGCCGCTCCGAT
TTTCCCAATCAGGTCAACAACGTCCTGTGCTTCCCCTATATCTTCCGTGGCGCGCTCGACTGCGGCGCCAGCGCCATCAA
CGAGGAGATGAAGATGGCCGCCGTGCGGGCCATCGCCGCCCTTGCCCGCGAAGAGCCTTCGGACGTCGCCGCACGCGCGT
ATTCGGGCGAGACGCCGATCTTCGGGCCCGATTTCCTGATCCCCTCGCCCTTCGATCCCAGGCTCATCCTGCGCATCGCG
CCGGCCGTCGCCAAGGCGGCCTGCGACACCGGTGTAGCGACGCGGCCGATCACCGACTTCGCCGCCTATATCGACAAGCT
CAACCGTTTCGTCTTCCGCTCCGGCCTGGTGATGAAGCCGGTGTTCTCGTCCGCCAAGGCATCGAGCGCCAAGCGCGTCA
TCTATGCCGATGGCGAGGATGAGCGCGTACTGCGCGCCGCCCAGGTGGTGCTCGAGGAAGGCATCGCTGAGCCGATCCTG
ATCGGCCGCCCACACGTCATCGAGGTCAGGCTGAAGCGCTACGGGCTGCGCATCAAGCCGGGCGTCGATTTCGGCCTGAT
CAACCCTGAGGACGATCCGCGCTATCGGCACTATGTCGACCTTTTGATCGAACTCGCCGGCCGGCGCGGGGTGACGACGG
AGGCGGCGCGCACCATGGTGCGCACCGACAACACGGTGATTGCGGCGCTGGCGCTCAAGCGTGGCGACGCCGATGCCATG
GTCTGCGGCCTCGAAGGCCGCTTCGAGCGGCATCTGCGCAACGTGACGCTGATCATCGGGCCGCGCGCGGGCATCAAGGA
CTATGACTTGTCGACGCTTTCGATGCTGATCTCGCAACGCGGAATCATCTTCCTCACTGACACGCACGTCTCCGTCGACC
CGACGGCCGAGGAGATCGCCGAGATGACCGTGCTGGCGGCCGAGGAAATCCAGCGCTTCGGCATCGAGCCGAAGGCAGCC
CTTCTGTCGCATTCGGATTTCGGTTCGCGCGATTCACCAAGCGCGTTGAAGATGCGCCAGGCGGCGGCGATCCTGGCGCG
CATCGCGCCGGAGTTGCAGTGCGACGGTGAAATGCATGCCGATTCCGCTTTGTCCGAGCATTTGCGCCAGCGCGTCTATC
CACATTCGCGGCTGAAGGGCGAAGCCAATCTGCTGGTGTTCCCGAACCTCGATTCGGCCAACATCACGCTGACGGCCCTG
CGCGCCATGATGGATGCGCTGCATGTCGGCCCGATCCTGCTCGGCACCGACAAGCCGGCGCACATACTGACGCCCTCGGT
CACGTCGCGCGGCGTGGTCAACATGACGGCGCTCGCAGTGGTCGAGGCCGCGCACAAGGCGCAGGCCATCGCCAATCTGG
ACTAG

Upstream 100 bases:

>100_bases
GCGCCGCCACTTAAAGCTGCCGGCGCGTCAATTAGCGTCGCGAAAAATCTTGGCCGCATGCTAAAAGGACTCCCGACACA
GGTTTCGAGGGAGCGGCCGC

Downstream 100 bases:

>100_bases
GCCGAAGCCGCCAGCAGGCTCGACCTGGTCTTCCAGGCCAGTGCTCTCATATGCCGGCAACAACTCGTTGCAAACCGGGG
TGTTGCGGACCGGCAACTTG

Product: malic enzyme

Products: NA

Alternate protein names: NAD-ME [H]

Number of amino acids: Translated: 774; Mature: 774

Protein sequence:

>774_residues
MEGENKKTARSDLDEAALYFHKHPTPGKLEIQATKPLGNQRDLALAYSPGVAAPCLEIRDNPATAADYTARANLVGVVSN
GSAVLGLGNIGPLASKPVMEGKAVLFKKFAGIDVFDIEIDAPEIERMVETVAALEPTFGGINLEDIKAPECFEVEERLKA
RMSIPVFHDDQHGTAIIVAAAVLNGLEFAGKTISDIKVVTSGAGAAALACLNLLVSLGVRIENIWVTDRFGVAYKGRTEE
MDRWKDPYVKDTEARTLADVIPGADVFLGLSAAGVLKPELLQHMAPKPLILALANPNPEIMPEVARAARPDAMICTGRSD
FPNQVNNVLCFPYIFRGALDCGASAINEEMKMAAVRAIAALAREEPSDVAARAYSGETPIFGPDFLIPSPFDPRLILRIA
PAVAKAACDTGVATRPITDFAAYIDKLNRFVFRSGLVMKPVFSSAKASSAKRVIYADGEDERVLRAAQVVLEEGIAEPIL
IGRPHVIEVRLKRYGLRIKPGVDFGLINPEDDPRYRHYVDLLIELAGRRGVTTEAARTMVRTDNTVIAALALKRGDADAM
VCGLEGRFERHLRNVTLIIGPRAGIKDYDLSTLSMLISQRGIIFLTDTHVSVDPTAEEIAEMTVLAAEEIQRFGIEPKAA
LLSHSDFGSRDSPSALKMRQAAAILARIAPELQCDGEMHADSALSEHLRQRVYPHSRLKGEANLLVFPNLDSANITLTAL
RAMMDALHVGPILLGTDKPAHILTPSVTSRGVVNMTALAVVEAAHKAQAIANLD

Sequences:

>Translated_774_residues
MEGENKKTARSDLDEAALYFHKHPTPGKLEIQATKPLGNQRDLALAYSPGVAAPCLEIRDNPATAADYTARANLVGVVSN
GSAVLGLGNIGPLASKPVMEGKAVLFKKFAGIDVFDIEIDAPEIERMVETVAALEPTFGGINLEDIKAPECFEVEERLKA
RMSIPVFHDDQHGTAIIVAAAVLNGLEFAGKTISDIKVVTSGAGAAALACLNLLVSLGVRIENIWVTDRFGVAYKGRTEE
MDRWKDPYVKDTEARTLADVIPGADVFLGLSAAGVLKPELLQHMAPKPLILALANPNPEIMPEVARAARPDAMICTGRSD
FPNQVNNVLCFPYIFRGALDCGASAINEEMKMAAVRAIAALAREEPSDVAARAYSGETPIFGPDFLIPSPFDPRLILRIA
PAVAKAACDTGVATRPITDFAAYIDKLNRFVFRSGLVMKPVFSSAKASSAKRVIYADGEDERVLRAAQVVLEEGIAEPIL
IGRPHVIEVRLKRYGLRIKPGVDFGLINPEDDPRYRHYVDLLIELAGRRGVTTEAARTMVRTDNTVIAALALKRGDADAM
VCGLEGRFERHLRNVTLIIGPRAGIKDYDLSTLSMLISQRGIIFLTDTHVSVDPTAEEIAEMTVLAAEEIQRFGIEPKAA
LLSHSDFGSRDSPSALKMRQAAAILARIAPELQCDGEMHADSALSEHLRQRVYPHSRLKGEANLLVFPNLDSANITLTAL
RAMMDALHVGPILLGTDKPAHILTPSVTSRGVVNMTALAVVEAAHKAQAIANLD
>Mature_774_residues
MEGENKKTARSDLDEAALYFHKHPTPGKLEIQATKPLGNQRDLALAYSPGVAAPCLEIRDNPATAADYTARANLVGVVSN
GSAVLGLGNIGPLASKPVMEGKAVLFKKFAGIDVFDIEIDAPEIERMVETVAALEPTFGGINLEDIKAPECFEVEERLKA
RMSIPVFHDDQHGTAIIVAAAVLNGLEFAGKTISDIKVVTSGAGAAALACLNLLVSLGVRIENIWVTDRFGVAYKGRTEE
MDRWKDPYVKDTEARTLADVIPGADVFLGLSAAGVLKPELLQHMAPKPLILALANPNPEIMPEVARAARPDAMICTGRSD
FPNQVNNVLCFPYIFRGALDCGASAINEEMKMAAVRAIAALAREEPSDVAARAYSGETPIFGPDFLIPSPFDPRLILRIA
PAVAKAACDTGVATRPITDFAAYIDKLNRFVFRSGLVMKPVFSSAKASSAKRVIYADGEDERVLRAAQVVLEEGIAEPIL
IGRPHVIEVRLKRYGLRIKPGVDFGLINPEDDPRYRHYVDLLIELAGRRGVTTEAARTMVRTDNTVIAALALKRGDADAM
VCGLEGRFERHLRNVTLIIGPRAGIKDYDLSTLSMLISQRGIIFLTDTHVSVDPTAEEIAEMTVLAAEEIQRFGIEPKAA
LLSHSDFGSRDSPSALKMRQAAAILARIAPELQCDGEMHADSALSEHLRQRVYPHSRLKGEANLLVFPNLDSANITLTAL
RAMMDALHVGPILLGTDKPAHILTPSVTSRGVVNMTALAVVEAAHKAQAIANLD

Specific function: Required for symbiotic nitrogen fixation. Plays a key role in the conversion of malate to acetyl-CoA for efficient tricarboxylic acid cycle function in nitrogen-fixating bacteria [H]

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=344, Percent_Identity=24.7093023255814, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI4505145, Length=351, Percent_Identity=23.9316239316239, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI62420882, Length=351, Percent_Identity=25.0712250712251, Blast_Score=75, Evalue=3e-13,
Organism=Homo sapiens, GI62420880, Length=351, Percent_Identity=25.0712250712251, Blast_Score=75, Evalue=3e-13,
Organism=Homo sapiens, GI239049447, Length=351, Percent_Identity=25.0712250712251, Blast_Score=75, Evalue=3e-13,
Organism=Homo sapiens, GI270265879, Length=318, Percent_Identity=24.8427672955975, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI1788806, Length=753, Percent_Identity=58.4329349269588, Blast_Score=888, Evalue=0.0,
Organism=Escherichia coli, GI1788635, Length=350, Percent_Identity=28, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1788800, Length=314, Percent_Identity=29.6178343949045, Blast_Score=122, Evalue=9e-29,
Organism=Escherichia coli, GI87081919, Length=300, Percent_Identity=25.3333333333333, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17537199, Length=359, Percent_Identity=25.0696378830084, Blast_Score=72, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322823, Length=269, Percent_Identity=27.8810408921933, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI281362674, Length=399, Percent_Identity=28.0701754385965, Blast_Score=84, Evalue=4e-16,
Organism=Drosophila melanogaster, GI281362672, Length=399, Percent_Identity=28.0701754385965, Blast_Score=84, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21356279, Length=399, Percent_Identity=28.0701754385965, Blast_Score=84, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.39 [H]

Molecular weight: Translated: 83455; Mature: 83455

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGENKKTARSDLDEAALYFHKHPTPGKLEIQATKPLGNQRDLALAYSPGVAAPCLEIRD
CCCCCCHHHHHHHHHHHHHEECCCCCCEEEEEEECCCCCCCCEEEEECCCCCCCCCEECC
NPATAADYTARANLVGVVSNGSAVLGLGNIGPLASKPVMEGKAVLFKKFAGIDVFDIEID
CCCCCCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEEC
APEIERMVETVAALEPTFGGINLEDIKAPECFEVEERLKARMSIPVFHDDQHGTAIIVAA
CHHHHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCEEECCCCCCEEEHHH
AVLNGLEFAGKTISDIKVVTSGAGAAALACLNLLVSLGVRIENIWVTDRFGVAYKGRTEE
HHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEEEEECCCEEECCCCHH
MDRWKDPYVKDTEARTLADVIPGADVFLGLSAAGVLKPELLQHMAPKPLILALANPNPEI
HHHHCCCCCCCCCHHHHHHHCCCCHHEEECCCCCCCCHHHHHHCCCCCEEEEEECCCCHH
MPEVARAARPDAMICTGRSDFPNQVNNVLCFPYIFRGALDCGASAINEEMKMAAVRAIAA
HHHHHHHCCCCEEEEECCCCCCHHHHCEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
LAREEPSDVAARAYSGETPIFGPDFLIPSPFDPRLILRIAPAVAKAACDTGVATRPITDF
HHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHCCCCCCCCHHHH
AAYIDKLNRFVFRSGLVMKPVFSSAKASSAKRVIYADGEDERVLRAAQVVLEEGIAEPIL
HHHHHHHHHHHHHCCCEEHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCEE
IGRPHVIEVRLKRYGLRIKPGVDFGLINPEDDPRYRHYVDLLIELAGRRGVTTEAARTMV
ECCCCEEEEEEHHCCEEECCCCCEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHH
RTDNTVIAALALKRGDADAMVCGLEGRFERHLRNVTLIIGPRAGIKDYDLSTLSMLISQR
HCCCEEEEEEEEECCCCCEEEECCCHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHCC
GIIFLTDTHVSVDPTAEEIAEMTVLAAEEIQRFGIEPKAALLSHSDFGSRDSPSALKMRQ
CEEEEEECCEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCHHHHHHH
AAAILARIAPELQCDGEMHADSALSEHLRQRVYPHSRLKGEANLLVFPNLDSANITLTAL
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHCCCCCCEEEECCCCCCCCHHHHH
RAMMDALHVGPILLGTDKPAHILTPSVTSRGVVNMTALAVVEAAHKAQAIANLD
HHHHHHHHCCCEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEGENKKTARSDLDEAALYFHKHPTPGKLEIQATKPLGNQRDLALAYSPGVAAPCLEIRD
CCCCCCHHHHHHHHHHHHHEECCCCCCEEEEEEECCCCCCCCEEEEECCCCCCCCCEECC
NPATAADYTARANLVGVVSNGSAVLGLGNIGPLASKPVMEGKAVLFKKFAGIDVFDIEID
CCCCCCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEEC
APEIERMVETVAALEPTFGGINLEDIKAPECFEVEERLKARMSIPVFHDDQHGTAIIVAA
CHHHHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCEEECCCCCCEEEHHH
AVLNGLEFAGKTISDIKVVTSGAGAAALACLNLLVSLGVRIENIWVTDRFGVAYKGRTEE
HHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEEEEECCCEEECCCCHH
MDRWKDPYVKDTEARTLADVIPGADVFLGLSAAGVLKPELLQHMAPKPLILALANPNPEI
HHHHCCCCCCCCCHHHHHHHCCCCHHEEECCCCCCCCHHHHHHCCCCCEEEEEECCCCHH
MPEVARAARPDAMICTGRSDFPNQVNNVLCFPYIFRGALDCGASAINEEMKMAAVRAIAA
HHHHHHHCCCCEEEEECCCCCCHHHHCEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
LAREEPSDVAARAYSGETPIFGPDFLIPSPFDPRLILRIAPAVAKAACDTGVATRPITDF
HHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHCCCCCCCCHHHH
AAYIDKLNRFVFRSGLVMKPVFSSAKASSAKRVIYADGEDERVLRAAQVVLEEGIAEPIL
HHHHHHHHHHHHHCCCEEHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCEE
IGRPHVIEVRLKRYGLRIKPGVDFGLINPEDDPRYRHYVDLLIELAGRRGVTTEAARTMV
ECCCCEEEEEEHHCCEEECCCCCEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHH
RTDNTVIAALALKRGDADAMVCGLEGRFERHLRNVTLIIGPRAGIKDYDLSTLSMLISQR
HCCCEEEEEEEEECCCCCEEEECCCHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHCC
GIIFLTDTHVSVDPTAEEIAEMTVLAAEEIQRFGIEPKAALLSHSDFGSRDSPSALKMRQ
CEEEEEECCEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCHHHHHHH
AAAILARIAPELQCDGEMHADSALSEHLRQRVYPHSRLKGEANLLVFPNLDSANITLTAL
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHCCCCCCEEEECCCCCCCCHHHHH
RAMMDALHVGPILLGTDKPAHILTPSVTSRGVVNMTALAVVEAAHKAQAIANLD
HHHHHHHHCCCEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9535928; 11481430; 10407149 [H]