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The map label for this gene is lexA

Identifier: 13470826

GI number: 13470826

Start: 491979

End: 492716

Strand: Direct

Name: lexA

Synonym: mlr0626

Alternate gene names: 13470826

Gene position: 491979-492716 (Clockwise)

Preceding gene: 13470825

Following gene: 13470828

Centisome position: 6.99

GC content: 62.47

Gene sequence:

>738_bases
ATGCTGACACGCAAGCAACATGAACTGTTGATGTTCATTCATGAACGGCTCAAGGAAAGCGGCATTCCGCCGTCTTTCGA
CGAGATGAAGGAAGCGCTTGATCTCGCCTCCAAGTCAGGCATTCATCGCCTGATCACGGCACTTGAGGAACGCGGCTTCA
TTCGCCGGCTGCCCAACCGGGCGCGGGCGCTGGAAGTGCTGCGGCTGCCGGATTCAATCGCGCCCGGCCTTAACGCGGCA
AAGAAATTCTCGCCAAGCGTCATCCAGGGCAGCCTCGGCCAGGGTGGTCTTGGCCGCCAGATCAAGCCGGCGCCGTCGCG
CTTGCCCGCGGCCGGCAATGATGACGACGCGGTTTCCGCCGTGTCGATCCCGGTGATGGGTCGCATCGCCGCTGGTGTGC
CGATCGATGCCATCCAGCATCAGACACATTCGATCTCGGTACCGCCGGACATGATCATGGGTGGCGAGCATTATGCCCTT
GAGGTGAAGGGCGACTCGATGATCGACGCCGGTATCTTCGACGGCGACACGGTCATCATCCGCAACGCCGACACGGCGAG
CCCGGGCGAGATCGTCGTGGCTTTGGTCGACGAGGAGGAAGCAACCCTGAAACGGTTCCGCCGCAAGGGCGCCTCGATCG
CCCTTGAAGCCGCCAACCCGGCCTACGAGACGCGCATTTTCGGACCGGACAGGGTCAAGGTGCAAGGCAAGCTGGTCGGG
CTGATCCGGCGCTACTGA

Upstream 100 bases:

>100_bases
TTTAAACAGTTGCAGAACACAACTGGAACAGATAGTGTTTGTTCTGGGTTTGTTTTTCGATTCTGGTTCACACCCTTCGT
CCAATGCCTTGGGGGCCGCC

Downstream 100 bases:

>100_bases
GCAGGAGCGGCCGGCTTTTCGGCTTTTTGATAGGGCGGCAGCCCCCTCGCCTCACGCGAAAATTTTCGCTGCTCGTGCCA
AGGCCGATACGGCCCGTCCA

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MLTRKQHELLMFIHERLKESGIPPSFDEMKEALDLASKSGIHRLITALEERGFIRRLPNRARALEVLRLPDSIAPGLNAA
KKFSPSVIQGSLGQGGLGRQIKPAPSRLPAAGNDDDAVSAVSIPVMGRIAAGVPIDAIQHQTHSISVPPDMIMGGEHYAL
EVKGDSMIDAGIFDGDTVIIRNADTASPGEIVVALVDEEEATLKRFRRKGASIALEAANPAYETRIFGPDRVKVQGKLVG
LIRRY

Sequences:

>Translated_245_residues
MLTRKQHELLMFIHERLKESGIPPSFDEMKEALDLASKSGIHRLITALEERGFIRRLPNRARALEVLRLPDSIAPGLNAA
KKFSPSVIQGSLGQGGLGRQIKPAPSRLPAAGNDDDAVSAVSIPVMGRIAAGVPIDAIQHQTHSISVPPDMIMGGEHYAL
EVKGDSMIDAGIFDGDTVIIRNADTASPGEIVVALVDEEEATLKRFRRKGASIALEAANPAYETRIFGPDRVKVQGKLVG
LIRRY
>Mature_245_residues
MLTRKQHELLMFIHERLKESGIPPSFDEMKEALDLASKSGIHRLITALEERGFIRRLPNRARALEVLRLPDSIAPGLNAA
KKFSPSVIQGSLGQGGLGRQIKPAPSRLPAAGNDDDAVSAVSIPVMGRIAAGVPIDAIQHQTHSISVPPDMIMGGEHYAL
EVKGDSMIDAGIFDGDTVIIRNADTASPGEIVVALVDEEEATLKRFRRKGASIALEAANPAYETRIFGPDRVKVQGKLVG
LIRRY

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=244, Percent_Identity=23.3606557377049, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_RHILO (Q98MD2)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_102395.1
- ProteinModelPortal:   Q98MD2
- SMR:   Q98MD2
- MEROPS:   S24.001
- GeneID:   1225058
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr0626
- NMPDR:   fig|266835.1.peg.500
- HOGENOM:   HBG679610
- OMA:   LMFIHER
- ProtClustDB:   PRK00215
- BRENDA:   3.4.21.88
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 26496; Mature: 26496

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: NA

Important sites: ACT_SITE 166-166 ACT_SITE 204-204

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRKQHELLMFIHERLKESGIPPSFDEMKEALDLASKSGIHRLITALEERGFIRRLPNR
CCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHCCCH
ARALEVLRLPDSIAPGLNAAKKFSPSVIQGSLGQGGLGRQIKPAPSRLPAAGNDDDAVSA
HHHHHHHHCCCHHCCCCCHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
VSIPVMGRIAAGVPIDAIQHQTHSISVPPDMIMGGEHYALEVKGDSMIDAGIFDGDTVII
EECCHHHHHHCCCCHHHHHCCCCEECCCHHHEECCCEEEEEECCCCEEECCCCCCCEEEE
RNADTASPGEIVVALVDEEEATLKRFRRKGASIALEAANPAYETRIFGPDRVKVQGKLVG
ECCCCCCCCEEEEEEECCCHHHHHHHHHCCCCEEEECCCCCCEEEECCCCCEEEHHHHHH
LIRRY
HHHCC
>Mature Secondary Structure
MLTRKQHELLMFIHERLKESGIPPSFDEMKEALDLASKSGIHRLITALEERGFIRRLPNR
CCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHCCCH
ARALEVLRLPDSIAPGLNAAKKFSPSVIQGSLGQGGLGRQIKPAPSRLPAAGNDDDAVSA
HHHHHHHHCCCHHCCCCCHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
VSIPVMGRIAAGVPIDAIQHQTHSISVPPDMIMGGEHYALEVKGDSMIDAGIFDGDTVII
EECCHHHHHHCCCCHHHHHCCCCEECCCHHHEECCCEEEEEECCCCEEECCCCCCCEEEE
RNADTASPGEIVVALVDEEEATLKRFRRKGASIALEAANPAYETRIFGPDRVKVQGKLVG
ECCCCCCCCEEEEEEECCCHHHHHHHHHCCCCEEEECCCCCCEEEECCCCCEEEHHHHHH
LIRRY
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968