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The map label for this gene is gltX

Identifier: 13470828

GI number: 13470828

Start: 495266

End: 496690

Strand: Direct

Name: gltX

Synonym: mlr0628

Alternate gene names: 13470828

Gene position: 495266-496690 (Clockwise)

Preceding gene: 13470826

Following gene: 13470829

Centisome position: 7.04

GC content: 66.74

Gene sequence:

>1425_bases
ATGTCCGACAAGGTCGTCACCCGTTTTGCCCCCTCGCCCACCGGCTATCTGCATATCGGCGGCGCGCGCACGGCGCTGTT
CAACTGGCTCTATGCCAAGCACACCGGCGGCACCATGCTGCTGCGCATCGAGGACACCGACCGCGAGCGTTCGACCGCCG
CCGCCACGGCCGCCATCCTCGACGGCCTCTCCTGGCTTGGTCTTGGCTGGGACGGCGACGCGGTCTCGCAATTTGAGCGC
GCGCCACGCCACCGCGAGGTCGCGGAAGAACTCGTTCGCATGGGCAAGGCCTATTACAGCTACGAAACGCCGGCCGAGCT
GGAGGCGATGCGCGAGGCAGCACGGGCCAAGGGCCTGCCGCCGCGCTACAACGGCCAGTGGCGCGACCGCACCCCCTCAG
AGGCGCCTGCAGGCGTCAAGGGCGCCATCCGCATCAAGGCGCCGACTGAGGGCGAGACGATCGTCCACGATCGTGTGCAG
GGCGAAGTGCGCTTCCCCAACAAGGATCTCGACGACTTCATCATCCTGCGTTCGGACGGCAACCCGACCTACATGCATGC
CGTCGTCGTCGACGACCACGACATGGGTGTCACGCATATCATCCGCGGCGACGACCACCTGACCAATGCCGCCCGCCAGA
CCGTGATCTACAACGCCATGGGCTGGGACGTGCCGTCCATGTCGCACATCCCGCTGATCCATGGCGCAGACGGCGCCAAG
CTGTCGAAGCGCCACGGCGCGCTCGGCGTCGAAGCCTATCGAGCCATGGGCTACCTGCCGGAGGCATTGCTCAACTATCT
CGCGCGCCTGGGCTGGAGCCACGGCGATGACGAGATCATGTCGATCAAGGACATGATCTCCTGGTTCGATATCGGCGACG
TCAACAAGGGCGCTGCCCGCTTCGACTTCGCCAAGCTGGAGGCGATCAACGGCGCACATATGCGCCGCATGGCCGACGCG
CAGCTGTTGGACATCTTCATCGCCACTTTGCCCTATCTCGAGGGCGGCCCGGCGATGGCCGCGCGCCTCAACGAACACAA
CAAGGCGCAGTTGCTGGCGGCCCTTCCGGGTCTGAAGGAGCGCGCCAAGACGCTGGTCGAGCTCGTCGATGGCGCCGCCT
TCCTGTTCGCCACGCGCCCGCTGCCGGTCGACGAGAAGGCGGCCCTCCTGCTCAATGACGGTGCCCGCAAGATCCTGCGC
GGCGCTCACGAAGCTTTGAACGCGCTTTCCGGCGACTGGACGGCGGCCGCGGCGGAAGCCGCGGTCCGCGACTATGCGCT
GGCCGGTGGCCACAAGCTTGGCGCCGTGGCCCAACCGTTGCGGGCCGCGCTGACCGGCAAAAGCACCTCGCCCGGCGTGT
TCGACGTGCTTGCCGTGCTTGGGCGCGAGGAAAGCCTGGCGCGCATAGCGGATCAAATCGATTAG

Upstream 100 bases:

>100_bases
GGTCTGCCCCTTGCACCCCTGACGCCCTATGCTACATGAACGCGCGACGCGCGAAAGTCCGCGCAGCCGCACCCCGCTTC
AGATGGTTTCCGAGAGTTTC

Downstream 100 bases:

>100_bases
GAGCAAACTGGCCCAAGAAGATTGGCATTGAAAGGCGCGTTTCGCAGTGCAACATTAGGCCTTGGCCCAATCTGGCACGC
ACCTCCTATAATGCGGTGGC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSDKVVTRFAPSPTGYLHIGGARTALFNWLYAKHTGGTMLLRIEDTDRERSTAAATAAILDGLSWLGLGWDGDAVSQFER
APRHREVAEELVRMGKAYYSYETPAELEAMREAARAKGLPPRYNGQWRDRTPSEAPAGVKGAIRIKAPTEGETIVHDRVQ
GEVRFPNKDLDDFIILRSDGNPTYMHAVVVDDHDMGVTHIIRGDDHLTNAARQTVIYNAMGWDVPSMSHIPLIHGADGAK
LSKRHGALGVEAYRAMGYLPEALLNYLARLGWSHGDDEIMSIKDMISWFDIGDVNKGAARFDFAKLEAINGAHMRRMADA
QLLDIFIATLPYLEGGPAMAARLNEHNKAQLLAALPGLKERAKTLVELVDGAAFLFATRPLPVDEKAALLLNDGARKILR
GAHEALNALSGDWTAAAAEAAVRDYALAGGHKLGAVAQPLRAALTGKSTSPGVFDVLAVLGREESLARIADQID

Sequences:

>Translated_474_residues
MSDKVVTRFAPSPTGYLHIGGARTALFNWLYAKHTGGTMLLRIEDTDRERSTAAATAAILDGLSWLGLGWDGDAVSQFER
APRHREVAEELVRMGKAYYSYETPAELEAMREAARAKGLPPRYNGQWRDRTPSEAPAGVKGAIRIKAPTEGETIVHDRVQ
GEVRFPNKDLDDFIILRSDGNPTYMHAVVVDDHDMGVTHIIRGDDHLTNAARQTVIYNAMGWDVPSMSHIPLIHGADGAK
LSKRHGALGVEAYRAMGYLPEALLNYLARLGWSHGDDEIMSIKDMISWFDIGDVNKGAARFDFAKLEAINGAHMRRMADA
QLLDIFIATLPYLEGGPAMAARLNEHNKAQLLAALPGLKERAKTLVELVDGAAFLFATRPLPVDEKAALLLNDGARKILR
GAHEALNALSGDWTAAAAEAAVRDYALAGGHKLGAVAQPLRAALTGKSTSPGVFDVLAVLGREESLARIADQID
>Mature_473_residues
SDKVVTRFAPSPTGYLHIGGARTALFNWLYAKHTGGTMLLRIEDTDRERSTAAATAAILDGLSWLGLGWDGDAVSQFERA
PRHREVAEELVRMGKAYYSYETPAELEAMREAARAKGLPPRYNGQWRDRTPSEAPAGVKGAIRIKAPTEGETIVHDRVQG
EVRFPNKDLDDFIILRSDGNPTYMHAVVVDDHDMGVTHIIRGDDHLTNAARQTVIYNAMGWDVPSMSHIPLIHGADGAKL
SKRHGALGVEAYRAMGYLPEALLNYLARLGWSHGDDEIMSIKDMISWFDIGDVNKGAARFDFAKLEAINGAHMRRMADAQ
LLDIFIATLPYLEGGPAMAARLNEHNKAQLLAALPGLKERAKTLVELVDGAAFLFATRPLPVDEKAALLLNDGARKILRG
AHEALNALSGDWTAAAAEAAVRDYALAGGHKLGAVAQPLRAALTGKSTSPGVFDVLAVLGREESLARIADQID

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=495, Percent_Identity=30.7070707070707, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI4826960, Length=301, Percent_Identity=27.906976744186, Blast_Score=76, Evalue=8e-14,
Organism=Homo sapiens, GI62241042, Length=237, Percent_Identity=28.6919831223629, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1788743, Length=471, Percent_Identity=41.6135881104034, Blast_Score=352, Evalue=2e-98,
Organism=Escherichia coli, GI226510925, Length=249, Percent_Identity=38.1526104417671, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1786895, Length=130, Percent_Identity=33.8461538461538, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71993394, Length=487, Percent_Identity=29.3634496919918, Blast_Score=181, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI17511007, Length=238, Percent_Identity=26.4705882352941, Blast_Score=75, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6324540, Length=491, Percent_Identity=30.9572301425662, Blast_Score=225, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6324742, Length=135, Percent_Identity=34.8148148148148, Blast_Score=76, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6321192, Length=226, Percent_Identity=30.0884955752212, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24665321, Length=489, Percent_Identity=31.0838445807771, Blast_Score=230, Evalue=2e-60,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE1_RHILO (Q98MD0)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_102397.1
- ProteinModelPortal:   Q98MD0
- SMR:   Q98MD0
- GeneID:   1225060
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr0628
- NMPDR:   fig|266835.1.peg.502
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- ProtClustDB:   PRK01406
- BRENDA:   6.1.1.17
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 51492; Mature: 51361

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 243-243

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKVVTRFAPSPTGYLHIGGARTALFNWLYAKHTGGTMLLRIEDTDRERSTAAATAAIL
CCCCEEEEECCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHH
DGLSWLGLGWDGDAVSQFERAPRHREVAEELVRMGKAYYSYETPAELEAMREAARAKGLP
HCHHHEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCC
PRYNGQWRDRTPSEAPAGVKGAIRIKAPTEGETIVHDRVQGEVRFPNKDLDDFIILRSDG
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCEEECCCCCCCCEEEEECCC
NPTYMHAVVVDDHDMGVTHIIRGDDHLTNAARQTVIYNAMGWDVPSMSHIPLIHGADGAK
CCEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHEEEECCCCCCCCCCCCCEEECCCCCC
LSKRHGALGVEAYRAMGYLPEALLNYLARLGWSHGDDEIMSIKDMISWFDIGDVNKGAAR
HHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHH
FDFAKLEAINGAHMRRMADAQLLDIFIATLPYLEGGPAMAARLNEHNKAQLLAALPGLKE
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHCCCHHH
RAKTLVELVDGAAFLFATRPLPVDEKAALLLNDGARKILRGAHEALNALSGDWTAAAAEA
HHHHHHHHHCCCEEEEECCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHH
AVRDYALAGGHKLGAVAQPLRAALTGKSTSPGVFDVLAVLGREESLARIADQID
HHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure 
SDKVVTRFAPSPTGYLHIGGARTALFNWLYAKHTGGTMLLRIEDTDRERSTAAATAAIL
CCCEEEEECCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHH
DGLSWLGLGWDGDAVSQFERAPRHREVAEELVRMGKAYYSYETPAELEAMREAARAKGLP
HCHHHEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCC
PRYNGQWRDRTPSEAPAGVKGAIRIKAPTEGETIVHDRVQGEVRFPNKDLDDFIILRSDG
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCEEECCCCCCCCEEEEECCC
NPTYMHAVVVDDHDMGVTHIIRGDDHLTNAARQTVIYNAMGWDVPSMSHIPLIHGADGAK
CCEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHEEEECCCCCCCCCCCCCEEECCCCCC
LSKRHGALGVEAYRAMGYLPEALLNYLARLGWSHGDDEIMSIKDMISWFDIGDVNKGAAR
HHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHH
FDFAKLEAINGAHMRRMADAQLLDIFIATLPYLEGGPAMAARLNEHNKAQLLAALPGLKE
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHCCCHHH
RAKTLVELVDGAAFLFATRPLPVDEKAALLLNDGARKILRGAHEALNALSGDWTAAAAEA
HHHHHHHHHCCCEEEEECCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHH
AVRDYALAGGHKLGAVAQPLRAALTGKSTSPGVFDVLAVLGREESLARIADQID
HHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968