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The map label for this gene is rimO

Identifier: 13470719

GI number: 13470719

Start: 367148

End: 368461

Strand: Direct

Name: rimO

Synonym: mlr0499

Alternate gene names: 13470719

Gene position: 367148-368461 (Clockwise)

Preceding gene: 13470718

Following gene: 13470728

Centisome position: 5.22

GC content: 63.55

Gene sequence:

>1314_bases
ATGTCCGCCCCTCGCGTCAGTTTCGTCAGCCTTGGATGCCCGAAGGCGCTCGTGGATTCCGAGCGCATCATCACGCGCCT
GCGCGCCGAAGGCTATGAGATCGCCCGCAAGCATGACGGCGCCGATCTTGTCGTGGTCAACACCTGCGGTTTCCTGGACT
CCGCCCGCGACGAGTCGCTCAACGCCATCGGTTCCGCGCTTTCGGAAAACGGCAGAGTCATCGTCACCGGCTGCCTCGGC
GCCGAGCCGGACGTGATCCGCGAAAAGCACCCCAATGTGTTGGCGATCACCGGACCACAGGCTTATGAGAGCGTCATGGC
AGCCGTGCATGAGGCGGCCCCGCCCTCGCACGATCCCTATATCGATCTGCTGCCGCCGCAGGGCGTCAAGCTGACGCCGC
GCCACTATGCCTATCTCAAGATCTCGGAAGGCTGCAACAACCGCTGCACCTTCTGCATCATTCCCGCACTTCGCGGCGAT
CTCGTTTCGCGCCCGGCGGCCGATGTGCTGCGCGAAGCCGAAAAACTGGCCAAGGCCGGCGTCAAGGAACTGCTCGTCAT
CTCGCAGGACACCAGCGCCTACGGCATCGACATCAAGTACCAGACCTCTATGTTCGGCGACCGTGAAGTGCGCGCGAAAT
TCCTCGATCTGTCGGAGGAATTGGGCAAGCTCGGCATCTGGGTGCGGATGCACTATGTCTACCCCTACCCGCATGTCGCC
GACGTCATCCCGCTGATGGCCGAGGGCAAGATCCTTCCCTATCTCGACATCCCGTTCCAGCACGCCTCGCCGCAGGTGCT
GAAGAACATGCGCCGGCCGGCGCATGGCGAAAAGACGCTGGAGCGCATTCGCGGCTGGCGCGACGTGTGCCCTGACCTCG
CCATCCGCTCGACCTTCATCGTCGGCTTCCCCGGCGAGACAGACGAGGATTTCGAGATGCTGCTCGACTGGCTGGATGAG
GCAAAAATCGACCGTGCCGGCTGCTTCAAATACGAGCCGGTCAAAGGCGCCCGTTCCAACGACCTCGGCCTCGAGCAGGT
GCCGCAGGACGTCAAGGAAGCGCGCTGGCACCGCTTCATGCAGCGCCAGCAGAAGATTTCCGCGACGCAGCTGGCGAAAA
AGGTCGGCAAGCGCCTGCCGGTGCTGATCGACGAAGCGCATGGCACTTCGGCCAAGGGCCGCACCAAATACGATGCGCCC
GAGATCGACGGCTCGGTCCACATCCAGTCGCGCCGCCCGATGCGTGCCGGTGACATCGTCACCGTCAAGATCGAGCGAGC
CGACGCCTACGACCTCTACGGTTCGGCCGTCTGA

Upstream 100 bases:

>100_bases
CGCGCGTATTGGCTCCTGCCAAGCGCTACAGACCGACATTTACAGCGATTTGAGGCCGGACCAGCGTTTCGGCCCGTGTG
CCCCCATTGGAAACTTGCCT

Downstream 100 bases:

>100_bases
GCGTTCCGTCACTTTCTCCCGGCAAATGAAAAGGGCGCCTCGCGGCGCCCTTTTCATTCGAAATGTGATGCTCCGCTTAC
TGCGGCAGCTTGTCGTCGAC

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MSAPRVSFVSLGCPKALVDSERIITRLRAEGYEIARKHDGADLVVVNTCGFLDSARDESLNAIGSALSENGRVIVTGCLG
AEPDVIREKHPNVLAITGPQAYESVMAAVHEAAPPSHDPYIDLLPPQGVKLTPRHYAYLKISEGCNNRCTFCIIPALRGD
LVSRPAADVLREAEKLAKAGVKELLVISQDTSAYGIDIKYQTSMFGDREVRAKFLDLSEELGKLGIWVRMHYVYPYPHVA
DVIPLMAEGKILPYLDIPFQHASPQVLKNMRRPAHGEKTLERIRGWRDVCPDLAIRSTFIVGFPGETDEDFEMLLDWLDE
AKIDRAGCFKYEPVKGARSNDLGLEQVPQDVKEARWHRFMQRQQKISATQLAKKVGKRLPVLIDEAHGTSAKGRTKYDAP
EIDGSVHIQSRRPMRAGDIVTVKIERADAYDLYGSAV

Sequences:

>Translated_437_residues
MSAPRVSFVSLGCPKALVDSERIITRLRAEGYEIARKHDGADLVVVNTCGFLDSARDESLNAIGSALSENGRVIVTGCLG
AEPDVIREKHPNVLAITGPQAYESVMAAVHEAAPPSHDPYIDLLPPQGVKLTPRHYAYLKISEGCNNRCTFCIIPALRGD
LVSRPAADVLREAEKLAKAGVKELLVISQDTSAYGIDIKYQTSMFGDREVRAKFLDLSEELGKLGIWVRMHYVYPYPHVA
DVIPLMAEGKILPYLDIPFQHASPQVLKNMRRPAHGEKTLERIRGWRDVCPDLAIRSTFIVGFPGETDEDFEMLLDWLDE
AKIDRAGCFKYEPVKGARSNDLGLEQVPQDVKEARWHRFMQRQQKISATQLAKKVGKRLPVLIDEAHGTSAKGRTKYDAP
EIDGSVHIQSRRPMRAGDIVTVKIERADAYDLYGSAV
>Mature_436_residues
SAPRVSFVSLGCPKALVDSERIITRLRAEGYEIARKHDGADLVVVNTCGFLDSARDESLNAIGSALSENGRVIVTGCLGA
EPDVIREKHPNVLAITGPQAYESVMAAVHEAAPPSHDPYIDLLPPQGVKLTPRHYAYLKISEGCNNRCTFCIIPALRGDL
VSRPAADVLREAEKLAKAGVKELLVISQDTSAYGIDIKYQTSMFGDREVRAKFLDLSEELGKLGIWVRMHYVYPYPHVAD
VIPLMAEGKILPYLDIPFQHASPQVLKNMRRPAHGEKTLERIRGWRDVCPDLAIRSTFIVGFPGETDEDFEMLLDWLDEA
KIDRAGCFKYEPVKGARSNDLGLEQVPQDVKEARWHRFMQRQQKISATQLAKKVGKRLPVLIDEAHGTSAKGRTKYDAPE
IDGSVHIQSRRPMRAGDIVTVKIERADAYDLYGSAV

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=478, Percent_Identity=26.1506276150628, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI28872782, Length=478, Percent_Identity=26.1506276150628, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI93277076, Length=391, Percent_Identity=27.1099744245524, Blast_Score=129, Evalue=8e-30,
Organism=Escherichia coli, GI1787057, Length=435, Percent_Identity=63.9080459770115, Blast_Score=595, Evalue=1e-171,
Organism=Escherichia coli, GI1786882, Length=444, Percent_Identity=27.7027027027027, Blast_Score=144, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17553146, Length=442, Percent_Identity=25.1131221719457, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI71996771, Length=207, Percent_Identity=31.8840579710145, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI21356207, Length=485, Percent_Identity=25.5670103092783, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI19922432, Length=400, Percent_Identity=25.5, Blast_Score=108, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_RHILO (Q98MN9)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_102288.1
- ProteinModelPortal:   Q98MN9
- SMR:   Q98MN9
- GeneID:   1224951
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr0499
- NMPDR:   fig|266835.1.peg.393
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- ProtClustDB:   PRK14862
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 48552; Mature: 48421

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPRVSFVSLGCPKALVDSERIITRLRAEGYEIARKHDGADLVVVNTCGFLDSARDESL
CCCCCEEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEECCCCCCCCHHHH
NAIGSALSENGRVIVTGCLGAEPDVIREKHPNVLAITGPQAYESVMAAVHEAAPPSHDPY
HHHHHHHCCCCCEEEEECCCCCCHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCE
IDLLPPQGVKLTPRHYAYLKISEGCNNRCTFCIIPALRGDLVSRPAADVLREAEKLAKAG
EECCCCCCCEECCCEEEEEEECCCCCCCCEEEEEECCCCHHHCCCHHHHHHHHHHHHHCC
VKELLVISQDTSAYGIDIKYQTSMFGDREVRAKFLDLSEELGKLGIWVRMHYVYPYPHVA
HHHEEEEECCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCHH
DVIPLMAEGKILPYLDIPFQHASPQVLKNMRRPAHGEKTLERIRGWRDVCPDLAIRSTFI
HHHHHHHCCCCCEEECCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHCEEE
VGFPGETDEDFEMLLDWLDEAKIDRAGCFKYEPVKGARSNDLGLEQVPQDVKEARWHRFM
EECCCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHH
QRQQKISATQLAKKVGKRLPVLIDEAHGTSAKGRTKYDAPEIDGSVHIQSRRPMRAGDIV
HHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEE
TVKIERADAYDLYGSAV
EEEEECCCCCCCCCCCC
>Mature Secondary Structure 
SAPRVSFVSLGCPKALVDSERIITRLRAEGYEIARKHDGADLVVVNTCGFLDSARDESL
CCCCEEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEECCCCCCCCHHHH
NAIGSALSENGRVIVTGCLGAEPDVIREKHPNVLAITGPQAYESVMAAVHEAAPPSHDPY
HHHHHHHCCCCCEEEEECCCCCCHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCE
IDLLPPQGVKLTPRHYAYLKISEGCNNRCTFCIIPALRGDLVSRPAADVLREAEKLAKAG
EECCCCCCCEECCCEEEEEEECCCCCCCCEEEEEECCCCHHHCCCHHHHHHHHHHHHHCC
VKELLVISQDTSAYGIDIKYQTSMFGDREVRAKFLDLSEELGKLGIWVRMHYVYPYPHVA
HHHEEEEECCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCHH
DVIPLMAEGKILPYLDIPFQHASPQVLKNMRRPAHGEKTLERIRGWRDVCPDLAIRSTFI
HHHHHHHCCCCCEEECCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHCEEE
VGFPGETDEDFEMLLDWLDEAKIDRAGCFKYEPVKGARSNDLGLEQVPQDVKEARWHRFM
EECCCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHH
QRQQKISATQLAKKVGKRLPVLIDEAHGTSAKGRTKYDAPEIDGSVHIQSRRPMRAGDIV
HHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEE
TVKIERADAYDLYGSAV
EEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968