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The map label for this gene is dat [H]

Identifier: 13470635

GI number: 13470635

Start: 288507

End: 289370

Strand: Direct

Name: dat [H]

Synonym: mlr0401

Alternate gene names: 13470635

Gene position: 288507-289370 (Clockwise)

Preceding gene: 13470634

Following gene: 13470636

Centisome position: 4.1

GC content: 63.43

Gene sequence:

>864_bases
ATGCCGCGCATTGCCTATGTCAACGGGCGCTACGTCGCTCACGCGGACGCTTCCGTGCATATCGAGGATCGCGGCTATCA
GTTCGCCGATGGCGTCTACGAGGTCTGCGAGGTGGCGCGCGGCTTCATCGTCGACATGCCGCGCCATCTTGCCCGGCTCA
ACCGCTCGCTGACCGAACTGTCGATCGCCTGGCCGGTCGCGCGCAACGTGCTGCCGCTGATCCTGCGCGAGGTGGTCAAT
CGCAACCACGTCGTCAACGGCCTTGTCTATGTGCAGGTGACGCGTGGTGTCGCCAGCCGCGAGTTTGTTTTCCCGTCGGC
GGACACCAAGCCGTCATTGGTGGTCACAGCCAGAAAGGCCGATCCGGCGGCTGGAGCAAAGCGAGCCGAAACCGGGATCG
CGGTCATCACCGTGCCAGAGAATCGCTGGGACCGGGTCGATATCAAGACCGTCGGACTGCTGCCCAATGTATTGGCCAAG
CAAAAAGCCAAGGAAGCCGGTGCGCAGGAAGCATGGTTCGTCGACACCGACGGCAACGTCAAGGAAGGCGGCTCGTCGAA
CGCCTGGATCGTCACCCGGGATGGCGTCCTGGTCACCAGGCCGGCCGATCACGGCATCCTGCGCGGCATCACCCGCACGA
CGATGTTCGAAGTCGCGGCCAAGCTCGGCCTGAAGATCGAGGAGCGCGGTTTTTCCGTCGCCGAGGCCAAGGCGGCGCGC
GAGGCGTTCATCAGTTCCGCCACGACGATTGCCATGCCGGTCGTGGCCATTGACGGCGATCCGGTGGCCAACGGGCACCC
CGGCTCAGTGACACTTTCGTTACGGCAAGCCTTTTTTGACATTGCGGAAAAAAGTCCAGCCTGA

Upstream 100 bases:

>100_bases
GCGCGGAACCTGTACTGAACCGCGCGTGGTCCAAGCATTTGCCGGTTACGGCGGCGGCGGAATCGCATAAGAAAGCCCGA
GATTTTTCCAGGGGTCGCCA

Downstream 100 bases:

>100_bases
TAACCGCACAGGTGGAATGAACATCAATATATCTCGTTCTGCTTGTCGTTACTGACGACAAGACGGTGTTTGTGCGCTTG
ACATGTGCCCGTTAATTTGG

Product: D-amino acid aminotransferase

Products: NA

Alternate protein names: D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MPRIAYVNGRYVAHADASVHIEDRGYQFADGVYEVCEVARGFIVDMPRHLARLNRSLTELSIAWPVARNVLPLILREVVN
RNHVVNGLVYVQVTRGVASREFVFPSADTKPSLVVTARKADPAAGAKRAETGIAVITVPENRWDRVDIKTVGLLPNVLAK
QKAKEAGAQEAWFVDTDGNVKEGGSSNAWIVTRDGVLVTRPADHGILRGITRTTMFEVAAKLGLKIEERGFSVAEAKAAR
EAFISSATTIAMPVVAIDGDPVANGHPGSVTLSLRQAFFDIAEKSPA

Sequences:

>Translated_287_residues
MPRIAYVNGRYVAHADASVHIEDRGYQFADGVYEVCEVARGFIVDMPRHLARLNRSLTELSIAWPVARNVLPLILREVVN
RNHVVNGLVYVQVTRGVASREFVFPSADTKPSLVVTARKADPAAGAKRAETGIAVITVPENRWDRVDIKTVGLLPNVLAK
QKAKEAGAQEAWFVDTDGNVKEGGSSNAWIVTRDGVLVTRPADHGILRGITRTTMFEVAAKLGLKIEERGFSVAEAKAAR
EAFISSATTIAMPVVAIDGDPVANGHPGSVTLSLRQAFFDIAEKSPA
>Mature_286_residues
PRIAYVNGRYVAHADASVHIEDRGYQFADGVYEVCEVARGFIVDMPRHLARLNRSLTELSIAWPVARNVLPLILREVVNR
NHVVNGLVYVQVTRGVASREFVFPSADTKPSLVVTARKADPAAGAKRAETGIAVITVPENRWDRVDIKTVGLLPNVLAKQ
KAKEAGAQEAWFVDTDGNVKEGGSSNAWIVTRDGVLVTRPADHGILRGITRTTMFEVAAKLGLKIEERGFSVAEAKAARE
AFISSATTIAMPVVAIDGDPVANGHPGSVTLSLRQAFFDIAEKSPA

Specific function: Acts on the D-isomers of alanine, leucine, aspartate, glutamate, aminobutyrate, norvaline and asparagine. The enzyme transfers an amino group from a substrate D-amino acid to the pyridoxal phosphate cofactor to form pyridoxamine and an alpha- keto acid in

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=290, Percent_Identity=27.5862068965517, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1787338, Length=262, Percent_Identity=26.7175572519084, Blast_Score=71, Evalue=9e-14,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005784 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.21 [H]

Molecular weight: Translated: 31032; Mature: 30901

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRIAYVNGRYVAHADASVHIEDRGYQFADGVYEVCEVARGFIVDMPRHLARLNRSLTEL
CCCEEEECCEEEEECCCEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHE
SIAWPVARNVLPLILREVVNRNHVVNGLVYVQVTRGVASREFVFPSADTKPSLVVTARKA
EEHHHHHHHHHHHHHHHHHCCCCEECEEEEEEEECCCCCCEEECCCCCCCCCEEEEEECC
DPAAGAKRAETGIAVITVPENRWDRVDIKTVGLLPNVLAKQKAKEAGAQEAWFVDTDGNV
CCCCCCCHHHCCEEEEEECCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCC
KEGGSSNAWIVTRDGVLVTRPADHGILRGITRTTMFEVAAKLGLKIEERGFSVAEAKAAR
CCCCCCCCEEEEECCEEEECCCCCHHHHHHHHHHHHHHHHHHCCEEHHCCCCHHHHHHHH
EAFISSATTIAMPVVAIDGDPVANGHPGSVTLSLRQAFFDIAEKSPA
HHHHHCCCEEEEEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PRIAYVNGRYVAHADASVHIEDRGYQFADGVYEVCEVARGFIVDMPRHLARLNRSLTEL
CCEEEECCEEEEECCCEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHE
SIAWPVARNVLPLILREVVNRNHVVNGLVYVQVTRGVASREFVFPSADTKPSLVVTARKA
EEHHHHHHHHHHHHHHHHHCCCCEECEEEEEEEECCCCCCEEECCCCCCCCCEEEEEECC
DPAAGAKRAETGIAVITVPENRWDRVDIKTVGLLPNVLAKQKAKEAGAQEAWFVDTDGNV
CCCCCCCHHHCCEEEEEECCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCC
KEGGSSNAWIVTRDGVLVTRPADHGILRGITRTTMFEVAAKLGLKIEERGFSVAEAKAAR
CCCCCCCCEEEEECCEEEECCCCCHHHHHHHHHHHHHHHHHHCCEEHHCCCCHHHHHHHH
EAFISSATTIAMPVVAIDGDPVANGHPGSVTLSLRQAFFDIAEKSPA
HHHHHCCCEEEEEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]