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The map label for this gene is ntrX [H]

Identifier: 13470634

GI number: 13470634

Start: 287044

End: 288405

Strand: Direct

Name: ntrX [H]

Synonym: mlr0400

Alternate gene names: 13470634

Gene position: 287044-288405 (Clockwise)

Preceding gene: 13470633

Following gene: 13470635

Centisome position: 4.08

GC content: 63.66

Gene sequence:

>1362_bases
ATGGCGTCTGATATTCTCATCGTCGATGACGAGGAAGACATCCGCGAGCTCGTCGCAGGCATATTGAGCGACGAAGGTCA
CGAAACCCGCACGGCATTCGACGCCGACAGCGCCTTGGCGGCCATTGCCGACCGGGCACCGAGGTTGATTTTCCTCGACA
TCTGGCTGCAGGGCTCGCGCCTGGACGGCCTGGCGCTGCTCGACGAGATCAAGACCATGCATCCCACCCTGCCGGTGGTG
ATGATCTCCGGCCACGGCAACATCGAAACAGCGGTCTCCGCCATCCGACGTGGCGCCTATGATTTCATCGAGAAGCCGTT
CAAGGCCGACAGGCTGATTCTCATTGCCGAGCGTGCGCTGGAAACCTCGAAGTTGCGGCGCGAGGTTTCCGACCTCAAAC
AGCGCAGTGGCGAGACCTTCGACCTGATCGGCATGTCGTCGGCCATGAGCCAGCTGCGCCAGACCATCGAGCGCGTCGCG
CCGACCAACAGCCGTGTCATGATCATCGGCCCGTCCGGATCCGGCAAGGAACTGGCGGCGCGCGCCATTCACACGCTGTC
GGCCCGCAAGGGCGCGCCATTCGTGACGCTAAGCGCCGCCAACATCACGCCCGAGCGCATGGAGATCGAGCTGTTCGGCA
CCGAATCGAACGGTGTCGAGCGCAAGGTCGGCGCGCTGGAGGAGGCCCATCGCGGCATCCTCTACATCGACGAAGTGGCC
GACATGCCGCGCGAGACGCAAAACAAGATCCTGCGCGTGCTGGTCGAGCAGCAGTTCGAGCGGGTAGGGGGCACCAAGCG
GGTCAAGGTCGATGTCCGCATCATCTCGTCGACCTCGCAGAACCTGGAAGCCATGATCGCCGACGGGCGTTTCCGCGAAG
ATCTCTACCACCGGCTGGCGGTGGTTCCGGTGATGGTGCCGGGCCTGGCCGAGCGGCGCGAGGACATTCCCTATCTCGTT
GACAATTTCATGAAGCAGATCGCCCGCCAGGCCGGCATCAAGCCGCGCCGTATCGGCGATGACGCGCTTGCCGTGCTGCA
GGCGCACAATTGGCCCGGCAACGTCCGTCAGCTGCGCAACAATGTCGAGCGCCTGATGATTCTGGCGCGCGGCGACGACG
TCGATGCGCCGATCACCGCGGACCTGCTGCCGTCCGAAATCGGCGACGTCATGCCGCGAACGCCGAATCAGTCCGACCAG
CACATTATGGCGCTGCCGTTGCGCGAGGCCCGTGAACAGTTCGAGAAGGACTATCTGATCGCCCAGATCAACCGCTTCGG
CGGCAACATCTCGAAGACCGCCGAGTTCATCGGCATGGAGCGCTCGGCGCTGCACCGCAAACTGAAGTCACTGGGCGTCT
GA

Upstream 100 bases:

>100_bases
CGCGGGGCGATGATCAGCATCATCCTGCCGCTGGCGGCCGTCACGCCGTTGCGCGGCGAAGGCAAGACGGAACACGAAAG
AGAAACTGAAAAGGTCGGTA

Downstream 100 bases:

>100_bases
CGCGCGGAACCTGTACTGAACCGCGCGTGGTCCAAGCATTTGCCGGTTACGGCGGCGGCGGAATCGCATAAGAAAGCCCG
AGATTTTTCCAGGGGTCGCC

Product: nitrogen assimilation regulatory protein ntrX

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MASDILIVDDEEDIRELVAGILSDEGHETRTAFDADSALAAIADRAPRLIFLDIWLQGSRLDGLALLDEIKTMHPTLPVV
MISGHGNIETAVSAIRRGAYDFIEKPFKADRLILIAERALETSKLRREVSDLKQRSGETFDLIGMSSAMSQLRQTIERVA
PTNSRVMIIGPSGSGKELAARAIHTLSARKGAPFVTLSAANITPERMEIELFGTESNGVERKVGALEEAHRGILYIDEVA
DMPRETQNKILRVLVEQQFERVGGTKRVKVDVRIISSTSQNLEAMIADGRFREDLYHRLAVVPVMVPGLAERREDIPYLV
DNFMKQIARQAGIKPRRIGDDALAVLQAHNWPGNVRQLRNNVERLMILARGDDVDAPITADLLPSEIGDVMPRTPNQSDQ
HIMALPLREAREQFEKDYLIAQINRFGGNISKTAEFIGMERSALHRKLKSLGV

Sequences:

>Translated_453_residues
MASDILIVDDEEDIRELVAGILSDEGHETRTAFDADSALAAIADRAPRLIFLDIWLQGSRLDGLALLDEIKTMHPTLPVV
MISGHGNIETAVSAIRRGAYDFIEKPFKADRLILIAERALETSKLRREVSDLKQRSGETFDLIGMSSAMSQLRQTIERVA
PTNSRVMIIGPSGSGKELAARAIHTLSARKGAPFVTLSAANITPERMEIELFGTESNGVERKVGALEEAHRGILYIDEVA
DMPRETQNKILRVLVEQQFERVGGTKRVKVDVRIISSTSQNLEAMIADGRFREDLYHRLAVVPVMVPGLAERREDIPYLV
DNFMKQIARQAGIKPRRIGDDALAVLQAHNWPGNVRQLRNNVERLMILARGDDVDAPITADLLPSEIGDVMPRTPNQSDQ
HIMALPLREAREQFEKDYLIAQINRFGGNISKTAEFIGMERSALHRKLKSLGV
>Mature_452_residues
ASDILIVDDEEDIRELVAGILSDEGHETRTAFDADSALAAIADRAPRLIFLDIWLQGSRLDGLALLDEIKTMHPTLPVVM
ISGHGNIETAVSAIRRGAYDFIEKPFKADRLILIAERALETSKLRREVSDLKQRSGETFDLIGMSSAMSQLRQTIERVAP
TNSRVMIIGPSGSGKELAARAIHTLSARKGAPFVTLSAANITPERMEIELFGTESNGVERKVGALEEAHRGILYIDEVAD
MPRETQNKILRVLVEQQFERVGGTKRVKVDVRIISSTSQNLEAMIADGRFREDLYHRLAVVPVMVPGLAERREDIPYLVD
NFMKQIARQAGIKPRRIGDDALAVLQAHNWPGNVRQLRNNVERLMILARGDDVDAPITADLLPSEIGDVMPRTPNQSDQH
IMALPLREAREQFEKDYLIAQINRFGGNISKTAEFIGMERSALHRKLKSLGV

Specific function: Member of the two-component regulatory system ntrY/ntrX involved in the activation of nitrogen assimilatory genes such as glnA. NtrX is probably phosphorylated by ntrY and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=466, Percent_Identity=37.1244635193133, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI1788550, Length=461, Percent_Identity=34.9240780911063, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI1790437, Length=451, Percent_Identity=35.6984478935698, Blast_Score=260, Evalue=1e-70,
Organism=Escherichia coli, GI1788905, Length=451, Percent_Identity=33.2594235033259, Blast_Score=231, Evalue=6e-62,
Organism=Escherichia coli, GI1789087, Length=438, Percent_Identity=34.0182648401827, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1789233, Length=324, Percent_Identity=37.3456790123457, Blast_Score=201, Evalue=9e-53,
Organism=Escherichia coli, GI87082117, Length=323, Percent_Identity=35.6037151702786, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1786524, Length=328, Percent_Identity=36.280487804878, Blast_Score=181, Evalue=9e-47,
Organism=Escherichia coli, GI87082152, Length=320, Percent_Identity=34.375, Blast_Score=178, Evalue=5e-46,
Organism=Escherichia coli, GI87081872, Length=323, Percent_Identity=33.4365325077399, Blast_Score=161, Evalue=9e-41,
Organism=Escherichia coli, GI1787583, Length=336, Percent_Identity=31.25, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI87081858, Length=291, Percent_Identity=26.8041237113402, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1789828, Length=247, Percent_Identity=29.5546558704453, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786599, Length=115, Percent_Identity=37.3913043478261, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50402; Mature: 50270

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASDILIVDDEEDIRELVAGILSDEGHETRTAFDADSALAAIADRAPRLIFLDIWLQGSR
CCCCEEEECCHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHCCCCEEEEEEEEECCC
LDGLALLDEIKTMHPTLPVVMISGHGNIETAVSAIRRGAYDFIEKPFKADRLILIAERAL
CCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCHHHHHHCCCCCCCEEEEHHHHH
ETSKLRREVSDLKQRSGETFDLIGMSSAMSQLRQTIERVAPTNSRVMIIGPSGSGKELAA
HHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHH
RAIHTLSARKGAPFVTLSAANITPERMEIELFGTESNGVERKVGALEEAHRGILYIDEVA
HHHHHHHCCCCCCEEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEHHHH
DMPRETQNKILRVLVEQQFERVGGTKRVKVDVRIISSTSQNLEAMIADGRFREDLYHRLA
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCHHHHHCCCCHHHHHHHHHH
VVPVMVPGLAERREDIPYLVDNFMKQIARQAGIKPRRIGDDALAVLQAHNWPGNVRQLRN
HHHHHCCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHH
NVERLMILARGDDVDAPITADLLPSEIGDVMPRTPNQSDQHIMALPLREAREQFEKDYLI
HHHHEEEEECCCCCCCCCCHHCCHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHHH
AQINRFGGNISKTAEFIGMERSALHRKLKSLGV
HHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
ASDILIVDDEEDIRELVAGILSDEGHETRTAFDADSALAAIADRAPRLIFLDIWLQGSR
CCCEEEECCHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHCCCCEEEEEEEEECCC
LDGLALLDEIKTMHPTLPVVMISGHGNIETAVSAIRRGAYDFIEKPFKADRLILIAERAL
CCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCHHHHHHCCCCCCCEEEEHHHHH
ETSKLRREVSDLKQRSGETFDLIGMSSAMSQLRQTIERVAPTNSRVMIIGPSGSGKELAA
HHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHH
RAIHTLSARKGAPFVTLSAANITPERMEIELFGTESNGVERKVGALEEAHRGILYIDEVA
HHHHHHHCCCCCCEEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEHHHH
DMPRETQNKILRVLVEQQFERVGGTKRVKVDVRIISSTSQNLEAMIADGRFREDLYHRLA
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCHHHHHCCCCHHHHHHHHHH
VVPVMVPGLAERREDIPYLVDNFMKQIARQAGIKPRRIGDDALAVLQAHNWPGNVRQLRN
HHHHHCCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHH
NVERLMILARGDDVDAPITADLLPSEIGDVMPRTPNQSDQHIMALPLREAREQFEKDYLI
HHHHEEEEECCCCCCCCCCHHCCHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHHH
AQINRFGGNISKTAEFIGMERSALHRKLKSLGV
HHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]