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The map label for this gene is cca

Identifier: 15602112

GI number: 15602112

Start: 277695

End: 278957

Strand: Reverse

Name: cca

Synonym: PM0247

Alternate gene names: 15602112

Gene position: 278957-277695 (Counterclockwise)

Preceding gene: 15602113

Following gene: 15602111

Centisome position: 12.36

GC content: 43.31

Gene sequence:

>1263_bases
ATAAAAATCTATTTAGTTGGAGGTGCTGTGCGAGATCAGCTCCTCAATCTTGTTGTGAAAGATCGTGATTGGGTCGTAGT
TGGTGCAACGCCAGATGATTTACTATCACAAGGCTATCAACAAGTCGGCAAAGACTTTCCTGTTTTTCTGCATCCCCAAA
CAAAAGAAGAATATGCTCTCGCTAGAACAGAACGTAAAGCGGGTAGCGGCTATACTGGCTTCATTTGTGATTTTTCGCCT
ACAATTTCTTTGGAACAAGATTTAAGTCGACGTGATTTAACTATCAATGCACTCGCTCAAGATCTTGACGGAAAAATCTA
TGATTTTTATGGTGGTCTTACGGATTTAAAACAACGTCTTTTACGCCACGTTTCCCCCGCCTTTGCAGAAGATCCGCTAC
GCGTATTACGTGTGGCACGTTTTGCTGCTCGCTACCATGCTCTTGGCTTCACTATTGCATCTGAAACCCGCGAATTGATG
CAACAGTTATCACAAAGTGGTGAGCTGTCAAACCTAACCGCAGAACGTGTCTGGTTAGAAACAGAAAAAGCCTTATTAGA
ACCGCATCCTGAAGTGTATTTCCAAACGCTACAAGAGGTGGGTGCATTACAAGTACTGTTTCCTGAATTAGCTGCATTAC
AGGGCGTCCCCAATCCCGCGAAATACCATCCGGAAATAGATACGTTTGTGCATACTATGCTGGTCCTACAACAAGCAGTC
TTGTTGACAGAAAACACGGACAGTGACAAAAGTGCGGTACGTTTTGCTGCAATTTGTCATGATTTAGGCAAAGCCTTAAC
ACCAAAAGAAATATTGCCACATCATTATGGACATGAAAAAGCTGGTGTCATGCCGACAAGACGCTTATGTCAGCGCTTTA
AATTACCTCACGCAATTCAAGATTTTGCAGAACTTTGTTGTGAATATCATTCGCACATACACAAAGCCTTTGAATTACGT
GCGGAGACAATATTGAAATTATTCAATCGTCTAGATGTCTGGCGTAAGCCGGAGCGTTTTAAAGCACTTTTGTTAGTCTG
TATTGCAGACACGCGTGGTAGGACCGGATTTGAACAAGTTGACTATCCACAACGTGAATTTCTCTGGCAACTTTATCAAA
GTGCTCTGCAGGTCAACGTGCAAGACATCATCCAACAAGGTTTCCAGCAGCAAGCCATTCGTGATGAACTCAATCGCCGT
CGTATAATCGCGATTAAACAGACACGCGCGGAAATCCTCCCGCGCTTTACTAATCCGTGTTAA

Upstream 100 bases:

>100_bases
TCTCGGTGTGGGTTTATTGTTAGGTTTGTTAATCCCATTCATTCTACCAAAACGCCGTCGCAATAATGGTTGGGCATAAC
GATCAACGATGAACAACAAG

Downstream 100 bases:

>100_bases
GCTCGTAAAATAATCACAATTTTGAGTACTTTTATTGGTTATGAAAAAACAACATTTTCTTTTGCCTATCATTGCAAGTT
TCTTTTTATTTGCTTGTACC

Product: multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MKIYLVGGAVRDQLLNLVVKDRDWVVVGATPDDLLSQGYQQVGKDFPVFLHPQTKEEYALARTERKAGSGYTGFICDFSP
TISLEQDLSRRDLTINALAQDLDGKIYDFYGGLTDLKQRLLRHVSPAFAEDPLRVLRVARFAARYHALGFTIASETRELM
QQLSQSGELSNLTAERVWLETEKALLEPHPEVYFQTLQEVGALQVLFPELAALQGVPNPAKYHPEIDTFVHTMLVLQQAV
LLTENTDSDKSAVRFAAICHDLGKALTPKEILPHHYGHEKAGVMPTRRLCQRFKLPHAIQDFAELCCEYHSHIHKAFELR
AETILKLFNRLDVWRKPERFKALLLVCIADTRGRTGFEQVDYPQREFLWQLYQSALQVNVQDIIQQGFQQQAIRDELNRR
RIIAIKQTRAEILPRFTNPC

Sequences:

>Translated_420_residues
MKIYLVGGAVRDQLLNLVVKDRDWVVVGATPDDLLSQGYQQVGKDFPVFLHPQTKEEYALARTERKAGSGYTGFICDFSP
TISLEQDLSRRDLTINALAQDLDGKIYDFYGGLTDLKQRLLRHVSPAFAEDPLRVLRVARFAARYHALGFTIASETRELM
QQLSQSGELSNLTAERVWLETEKALLEPHPEVYFQTLQEVGALQVLFPELAALQGVPNPAKYHPEIDTFVHTMLVLQQAV
LLTENTDSDKSAVRFAAICHDLGKALTPKEILPHHYGHEKAGVMPTRRLCQRFKLPHAIQDFAELCCEYHSHIHKAFELR
AETILKLFNRLDVWRKPERFKALLLVCIADTRGRTGFEQVDYPQREFLWQLYQSALQVNVQDIIQQGFQQQAIRDELNRR
RIIAIKQTRAEILPRFTNPC
>Mature_420_residues
MKIYLVGGAVRDQLLNLVVKDRDWVVVGATPDDLLSQGYQQVGKDFPVFLHPQTKEEYALARTERKAGSGYTGFICDFSP
TISLEQDLSRRDLTINALAQDLDGKIYDFYGGLTDLKQRLLRHVSPAFAEDPLRVLRVARFAARYHALGFTIASETRELM
QQLSQSGELSNLTAERVWLETEKALLEPHPEVYFQTLQEVGALQVLFPELAALQGVPNPAKYHPEIDTFVHTMLVLQQAV
LLTENTDSDKSAVRFAAICHDLGKALTPKEILPHHYGHEKAGVMPTRRLCQRFKLPHAIQDFAELCCEYHSHIHKAFELR
AETILKLFNRLDVWRKPERFKALLLVCIADTRGRTGFEQVDYPQREFLWQLYQSALQVNVQDIIQQGFQQQAIRDELNRR
RIIAIKQTRAEILPRFTNPC

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily

Homologues:

Organism=Homo sapiens, GI155030240, Length=195, Percent_Identity=29.2307692307692, Blast_Score=69, Evalue=9e-12,
Organism=Escherichia coli, GI1789436, Length=415, Percent_Identity=59.2771084337349, Blast_Score=505, Evalue=1e-144,
Organism=Escherichia coli, GI87081691, Length=223, Percent_Identity=32.2869955156951, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71995920, Length=260, Percent_Identity=26.5384615384615, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6321016, Length=240, Percent_Identity=30.4166666666667, Blast_Score=73, Evalue=7e-14,
Organism=Drosophila melanogaster, GI281360112, Length=353, Percent_Identity=24.0793201133144, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI21357337, Length=353, Percent_Identity=24.0793201133144, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_PASMU (Q9CP21)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245184.1
- ProteinModelPortal:   Q9CP21
- SMR:   Q9CP21
- GeneID:   1243594
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0247
- NMPDR:   fig|272843.1.peg.247
- HOGENOM:   HBG635063
- OMA:   DRIWREW
- ProtClustDB:   PRK10885
- BioCyc:   PMUL272843:PM0247-MONOMER
- BRENDA:   2.7.7.21
- BRENDA:   2.7.7.25
- HAMAP:   MF_01261
- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646
- PIRSF:   PIRSF000813
- SMART:   SM00471

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 48090; Mature: 48090

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: BINDING 8-8 BINDING 11-11 BINDING 91-91 BINDING 137-137 BINDING 140-140

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIYLVGGAVRDQLLNLVVKDRDWVVVGATPDDLLSQGYQQVGKDFPVFLHPQTKEEYAL
CEEEEECHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCCCEEECCCCHHHHHH
ARTERKAGSGYTGFICDFSPTISLEQDLSRRDLTINALAQDLDGKIYDFYGGLTDLKQRL
HHHHHHCCCCCEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHCCCHHHHHCCHHHHHHHH
LRHVSPAFAEDPLRVLRVARFAARYHALGFTIASETRELMQQLSQSGELSNLTAERVWLE
HHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
TEKALLEPHPEVYFQTLQEVGALQVLFPELAALQGVPNPAKYHPEIDTFVHTMLVLQQAV
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
LLTENTDSDKSAVRFAAICHDLGKALTPKEILPHHYGHEKAGVMPTRRLCQRFKLPHAIQ
HHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCHHHHHHHHCCCCHHHH
DFAELCCEYHSHIHKAFELRAETILKLFNRLDVWRKPERFKALLLVCIADTRGRTGFEQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEECCCCCCCCHHC
DYPQREFLWQLYQSALQVNVQDIIQQGFQQQAIRDELNRRRIIAIKQTRAEILPRFTNPC
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKIYLVGGAVRDQLLNLVVKDRDWVVVGATPDDLLSQGYQQVGKDFPVFLHPQTKEEYAL
CEEEEECHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCCCEEECCCCHHHHHH
ARTERKAGSGYTGFICDFSPTISLEQDLSRRDLTINALAQDLDGKIYDFYGGLTDLKQRL
HHHHHHCCCCCEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHCCCHHHHHCCHHHHHHHH
LRHVSPAFAEDPLRVLRVARFAARYHALGFTIASETRELMQQLSQSGELSNLTAERVWLE
HHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
TEKALLEPHPEVYFQTLQEVGALQVLFPELAALQGVPNPAKYHPEIDTFVHTMLVLQQAV
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
LLTENTDSDKSAVRFAAICHDLGKALTPKEILPHHYGHEKAGVMPTRRLCQRFKLPHAIQ
HHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCHHHHHHHHCCCCHHHH
DFAELCCEYHSHIHKAFELRAETILKLFNRLDVWRKPERFKALLLVCIADTRGRTGFEQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEECCCCCCCCHHC
DYPQREFLWQLYQSALQVNVQDIIQQGFQQQAIRDELNRRRIIAIKQTRAEILPRFTNPC
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100