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The map label for this gene is lolB

Identifier: 15602111

GI number: 15602111

Start: 277037

End: 277660

Strand: Reverse

Name: lolB

Synonym: PM0246

Alternate gene names: 15602111

Gene position: 277660-277037 (Counterclockwise)

Preceding gene: 15602112

Following gene: 15602110

Centisome position: 12.3

GC content: 40.38

Gene sequence:

>624_bases
TTGGTTATGAAAAAACAACATTTTCTTTTGCCTATCATTGCAAGTTTCTTTTTATTTGCTTGTACCCTGGATACAGAACG
TCCAACGGATGTGAAATATATCAGTCACACCGATCCCACGTGGCAACAACATTTATCTCAACTTAAGAAGATTCGCGATT
ACACTAATCAAGGTCAGTTAGGTTATATCAGTCAGAAAGAACGATTTTCTACTCGTTTTGACTGGCAATACCAAAATCCA
ACCAACTACCGTCTAACCTTGTCTTCCACACTGAGCCCAACAACCCTATCTATTGAAGTACGTCATAATGTTATGCACCT
GTCTGATAATAAAGGACCTCTCCGCTCAGCACAGGATGCCAAGCGGTTACTGAAAGAGATTGTTGGGATGGATTTACCGC
TTGACCAATTCGCCCTTTGGTTAAAAGGACAGCCTGATGAGAGTCGGGAATATCGAGTCGCTGAAAATCACTTACTCGCC
CACTTTAGCTATCCTATCGATAATCAACAATGGACAGCGGATTACTTAAGCTATCATCAACTTCCACTTCCTTTACCGAA
GGATATTTTGTTGAAGACAGAAGGGCAAACTTTAAAGATTCGTATCGATAACTGGACTTATTAA

Upstream 100 bases:

>100_bases
CGTCGTATAATCGCGATTAAACAGACACGCGCGGAAATCCTCCCGCGCTTTACTAATCCGTGTTAAGCTCGTAAAATAAT
CACAATTTTGAGTACTTTTA

Downstream 100 bases:

>100_bases
TGCAACATTATCATTTTTCTCACTCACTTTTAGCACAACAAGAAAAACCATATCGTTTCCCTTGTCCGGCTAAACTTAAC
TTATTTCTCTACATTAATGG

Product: outer membrane lipoprotein LolB

Products: 5-aminolevulinate; CoA; CO2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MVMKKQHFLLPIIASFFLFACTLDTERPTDVKYISHTDPTWQQHLSQLKKIRDYTNQGQLGYISQKERFSTRFDWQYQNP
TNYRLTLSSTLSPTTLSIEVRHNVMHLSDNKGPLRSAQDAKRLLKEIVGMDLPLDQFALWLKGQPDESREYRVAENHLLA
HFSYPIDNQQWTADYLSYHQLPLPLPKDILLKTEGQTLKIRIDNWTY

Sequences:

>Translated_207_residues
MVMKKQHFLLPIIASFFLFACTLDTERPTDVKYISHTDPTWQQHLSQLKKIRDYTNQGQLGYISQKERFSTRFDWQYQNP
TNYRLTLSSTLSPTTLSIEVRHNVMHLSDNKGPLRSAQDAKRLLKEIVGMDLPLDQFALWLKGQPDESREYRVAENHLLA
HFSYPIDNQQWTADYLSYHQLPLPLPKDILLKTEGQTLKIRIDNWTY
>Mature_207_residues
MVMKKQHFLLPIIASFFLFACTLDTERPTDVKYISHTDPTWQQHLSQLKKIRDYTNQGQLGYISQKERFSTRFDWQYQNP
TNYRLTLSSTLSPTTLSIEVRHNVMHLSDNKGPLRSAQDAKRLLKEIVGMDLPLDQFALWLKGQPDESREYRVAENHLLA
HFSYPIDNQQWTADYLSYHQLPLPLPKDILLKTEGQTLKIRIDNWTY

Specific function: Plays a critical role in the incorporation of lipoproteins in the outer membrane after they are released by the lolA protein

COG id: COG3017

COG function: function code M; Outer membrane lipoprotein involved in outer membrane biogenesis

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lolB family

Homologues:

Organism=Escherichia coli, GI1787460, Length=198, Percent_Identity=29.7979797979798, Blast_Score=113, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LOLB_PASMU (P57834)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245183.1
- ProteinModelPortal:   P57834
- SMR:   P57834
- GeneID:   1243593
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0246
- NMPDR:   fig|272843.1.peg.246
- HOGENOM:   HBG296876
- OMA:   FSSRFEW
- ProtClustDB:   PRK00022
- BioCyc:   PMUL272843:PM0246-MONOMER
- HAMAP:   MF_00233
- InterPro:   IPR004565
- TIGRFAMs:   TIGR00548

Pfam domain/function: PF03550 LolB

EC number: NA

Molecular weight: Translated: 24396; Mature: 24396

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVMKKQHFLLPIIASFFLFACTLDTERPTDVKYISHTDPTWQQHLSQLKKIRDYTNQGQL
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCE
GYISQKERFSTRFDWQYQNPTNYRLTLSSTLSPTTLSIEVRHNVMHLSDNKGPLRSAQDA
EECCCHHHCCCEECEEECCCCCEEEEEECCCCCEEEEEEEEEEEEEEECCCCCCCCHHHH
KRLLKEIVGMDLPLDQFALWLKGQPDESREYRVAENHLLAHFSYPIDNQQWTADYLSYHQ
HHHHHHHHCCCCCHHHHEEEECCCCCCCCCEEECCCEEEEEEECCCCCCCHHHHHHHHCC
LPLPLPKDILLKTEGQTLKIRIDNWTY
CCCCCCHHEEEECCCCEEEEEECCCCC
>Mature Secondary Structure
MVMKKQHFLLPIIASFFLFACTLDTERPTDVKYISHTDPTWQQHLSQLKKIRDYTNQGQL
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCE
GYISQKERFSTRFDWQYQNPTNYRLTLSSTLSPTTLSIEVRHNVMHLSDNKGPLRSAQDA
EECCCHHHCCCEECEEECCCCCEEEEEECCCCCEEEEEEEEEEEEEEECCCCCCCCHHHH
KRLLKEIVGMDLPLDQFALWLKGQPDESREYRVAENHLLAHFSYPIDNQQWTADYLSYHQ
HHHHHHHHCCCCCHHHHEEEECCCCCCCCCEEECCCEEEEEEECCCCCCCHHHHHHHHCC
LPLPLPKDILLKTEGQTLKIRIDNWTY
CCCCCCHHEEEECCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Succinyl-CoA; glycine [C]

Specific reaction: Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 [C]

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11248100