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The map label for this gene is ipk

Identifier: 15602110

GI number: 15602110

Start: 276150

End: 277037

Strand: Reverse

Name: ipk

Synonym: PM0245

Alternate gene names: 15602110

Gene position: 277037-276150 (Counterclockwise)

Preceding gene: 15602111

Following gene: 15602109

Centisome position: 12.27

GC content: 38.96

Gene sequence:

>888_bases
ATGCAACATTATCATTTTTCTCACTCACTTTTAGCACAACAAGAAAAACCATATCGTTTCCCTTGTCCGGCTAAACTTAA
CTTATTTCTCTACATTAATGGGAAACGTCAAGATGGTTATCACGAACTACAAACCCTTTTTCAATTTGTTGATTTTGGTG
ATTGGTTAGATATTGAGGTTCGCGAAGATAACGAAATCTGCCTAACGCCAGAACTTCCCTCACTAAAAAATGAAGATAAT
TTAGTCTATCGCGCAGCAAAATTACTGCAACAGAAAACCAATTGTGCTTTAGGCGCCAATCTGACACTTGATAAGATTCT
ACCGATGGGAAGTGGGCTCGGAGGAGGCTCGTCTAATGCGGCAACAGCATTAGTCGCATTAAATTACCTGTGGAATACGC
AACTCAGCACAAAACAACTTGCTAAACTAGGATTAATGCTCGGTGCAGACGTACCAATTTTTGTCCATGGTCACGCGGCA
TTTGCCGAAGGAGTAGGTGAAAAAATTACTTATTGTGAGCCAAAAGAGAAATGGTATGTGGTTTTAAAGCCAAATGTGTC
TATATCAACCGCGACCGTCTTTTCTGATCCAGATTTAATTCGTAATACCCCTAAGCAATCTCTGGAGCAACTCCTCAATC
AAAAATATGCAAACGATTGTGAAAAAGTTGTGTTAAATCATTATCCAGAGGTTGAAGAAATACTCCACAGATTGTTACAA
TATGCGCCATCAAGATTAACAGGCACCGGCGCTTGTGTATTTGCCGAATTTAATGACGAAGAAAGTGCACAACTCGCATT
TCAAACAATACCCAAAAACTATTTTGGTTTTGTCGCACAAGGATTAAATAAATCACCTTTACATAATATGCTAGCGAAAA
TCAGCTAG

Upstream 100 bases:

>100_bases
ACTTAAGCTATCATCAACTTCCACTTCCTTTACCGAAGGATATTTTGTTGAAGACAGAAGGGCAAACTTTAAAGATTCGT
ATCGATAACTGGACTTATTA

Downstream 100 bases:

>100_bases
CTCGATAATAACGAATTTACTCAACCATTTTTATTTCTTTACAATCTAAACCTCGAGGTTCAATTAACAATGCCTGATAT
AAAACTCTTTACTGGTAATG

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MQHYHFSHSLLAQQEKPYRFPCPAKLNLFLYINGKRQDGYHELQTLFQFVDFGDWLDIEVREDNEICLTPELPSLKNEDN
LVYRAAKLLQQKTNCALGANLTLDKILPMGSGLGGGSSNAATALVALNYLWNTQLSTKQLAKLGLMLGADVPIFVHGHAA
FAEGVGEKITYCEPKEKWYVVLKPNVSISTATVFSDPDLIRNTPKQSLEQLLNQKYANDCEKVVLNHYPEVEEILHRLLQ
YAPSRLTGTGACVFAEFNDEESAQLAFQTIPKNYFGFVAQGLNKSPLHNMLAKIS

Sequences:

>Translated_295_residues
MQHYHFSHSLLAQQEKPYRFPCPAKLNLFLYINGKRQDGYHELQTLFQFVDFGDWLDIEVREDNEICLTPELPSLKNEDN
LVYRAAKLLQQKTNCALGANLTLDKILPMGSGLGGGSSNAATALVALNYLWNTQLSTKQLAKLGLMLGADVPIFVHGHAA
FAEGVGEKITYCEPKEKWYVVLKPNVSISTATVFSDPDLIRNTPKQSLEQLLNQKYANDCEKVVLNHYPEVEEILHRLLQ
YAPSRLTGTGACVFAEFNDEESAQLAFQTIPKNYFGFVAQGLNKSPLHNMLAKIS
>Mature_295_residues
MQHYHFSHSLLAQQEKPYRFPCPAKLNLFLYINGKRQDGYHELQTLFQFVDFGDWLDIEVREDNEICLTPELPSLKNEDN
LVYRAAKLLQQKTNCALGANLTLDKILPMGSGLGGGSSNAATALVALNYLWNTQLSTKQLAKLGLMLGADVPIFVHGHAA
FAEGVGEKITYCEPKEKWYVVLKPNVSISTATVFSDPDLIRNTPKQSLEQLLNQKYANDCEKVVLNHYPEVEEILHRLLQ
YAPSRLTGTGACVFAEFNDEESAQLAFQTIPKNYFGFVAQGLNKSPLHNMLAKIS

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=279, Percent_Identity=56.6308243727599, Blast_Score=312, Evalue=2e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_PASMU (P57833)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245182.1
- ProteinModelPortal:   P57833
- SMR:   P57833
- GeneID:   1243592
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0245
- NMPDR:   fig|272843.1.peg.245
- HOGENOM:   HBG734593
- OMA:   LNLFLHV
- ProtClustDB:   PRK00343
- BioCyc:   PMUL272843:PM0245-MONOMER
- BRENDA:   2.7.1.148
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 33064; Mature: 33064

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: ACT_SITE 25-25 ACT_SITE 150-150

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHYHFSHSLLAQQEKPYRFPCPAKLNLFLYINGKRQDGYHELQTLFQFVDFGDWLDIEV
CCCCCCHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEE
REDNEICLTPELPSLKNEDNLVYRAAKLLQQKTNCALGANLTLDKILPMGSGLGGGSSNA
ECCCCEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCHH
ATALVALNYLWNTQLSTKQLAKLGLMLGADVPIFVHGHAAFAEGVGEKITYCEPKEKWYV
HHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEECCHHHHHCCCCCCEEECCCCCEEE
VLKPNVSISTATVFSDPDLIRNTPKQSLEQLLNQKYANDCEKVVLNHYPEVEEILHRLLQ
EECCCCCEEEEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
YAPSRLTGTGACVFAEFNDEESAQLAFQTIPKNYFGFVAQGLNKSPLHNMLAKIS
HCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MQHYHFSHSLLAQQEKPYRFPCPAKLNLFLYINGKRQDGYHELQTLFQFVDFGDWLDIEV
CCCCCCHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEE
REDNEICLTPELPSLKNEDNLVYRAAKLLQQKTNCALGANLTLDKILPMGSGLGGGSSNA
ECCCCEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCHH
ATALVALNYLWNTQLSTKQLAKLGLMLGADVPIFVHGHAAFAEGVGEKITYCEPKEKWYV
HHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEECCHHHHHCCCCCCEEECCCCCEEE
VLKPNVSISTATVFSDPDLIRNTPKQSLEQLLNQKYANDCEKVVLNHYPEVEEILHRLLQ
EECCCCCEEEEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
YAPSRLTGTGACVFAEFNDEESAQLAFQTIPKNYFGFVAQGLNKSPLHNMLAKIS
HCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100