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The map label for this gene is lysS [H]

Identifier: 15602054

GI number: 15602054

Start: 211228

End: 212733

Strand: Reverse

Name: lysS [H]

Synonym: PM0189

Alternate gene names: 15602054

Gene position: 212733-211228 (Counterclockwise)

Preceding gene: 15602055

Following gene: 15602053

Centisome position: 9.42

GC content: 42.36

Gene sequence:

>1506_bases
ATGTCAGAACAAACTCAAGAACTTGATCTTAATGGCGAGATGTTAGTCCGCCGTGAAAAATTAGCCGCGTTGCGCGCTAA
AGGTAACCCATTTCCAAATACATTTCGTCGTGATGCGCTTGCACAAGATTTACACGCAAACTACGATGCACTTGAAGGCG
AAGCATTAAAAGCACAAGAAGTCGAAGTAAAAGTCGCTGGACGTATTATGACGCGTCGTGCAATGGGTAAGGCGACTTTT
ATCACCTTGCAAGATATGTCTGGTCGTATTCAACTCTATGTCGCACGTGATAATTTGCCAGAAGGTATTTATGCTGACGA
TGTGGGACAGTGGGATTTAGGCGATATCATTGGCGTAAAAGGCACCTTATTTAAAACCAAAACCAATGAATTAACCGTAC
GTTGTACTGAAGTGCAGTTATTAACCAAAGCATTACGCCCATTACCAGATAAATTCCATGGTCTAAGCGATCAAGAAACA
CGCTATCGTCAACGTTATTTAGATTTAATTGCAAACGAAGACTCACGTCGTACTTTCATTATTCGTTCTAAAGTGATTGC
GGGTATTCGTGAATATTTTCTGTCAAAAGGCTTTATGGAAGTAGAAACCCCGATGTTACAAATTATTCCGGGTGGGGCTT
CTGCACGTCCATTTGTAACACATCATAATGCATTAGATGTGGATATGTATTTACGTATTGCGCCTGAGCTTTATTTAAAA
CGCTTAGTGGTTGGTGGCTTTGAACGTGTCTTTGAATTAAACCGCAACTTCCGTAACGAAGGAGTGTCTGTTCGCCATAA
CCCAGAATTTACCATGCTCGAATACTACCAAGCCTATGCGGATTACCATGATTTAATGGATAATACTGAAGAATTGTTAC
GTAAACTGGCAATGGACATTTTAGGCACGACGATTGTCCCTTATGGTGAGCTTGAATTTGATTTTGGCAAACCATTTGAA
CGTATCACGATGCATGATGCCATTTTGAAATACGGCGCAGAAAAAGGTATCGTCAAAGAGGATCTATATGACTTTGATCG
TGCTGTTGCCGTTGCGAAAAAATTAGGTATTGAAGTACAAAAATCGTGGGGGTTAGGCAGTCTAGTCAATGTAATTTTTG
AAGAGGTGGCGGAGCATCACTTAATTCAGCCAACGTTCTTAATGGCACACCCTGCCGAAATTTCGCCATTAGCGCGTCGT
AATGATGAAAATCCAGAAGTCACTGACCGTTTTGAATTATTCATCGGTGGACGTGAAATTGGTAACGGTTTCTCTGAGTT
AAACGATGCCGAAGATCAAAACGAACGCTTTGACGCACAAGTTGCGGCGAAAGAAGCGGGGGATGATGAAGCGATGTTTA
AAGACGAAGATTTCGTGACTGCTCTTGAACATGGCTTACCACCAACAGCAGGCGAAGGGTTAGGTATCGACCGCTTAGCG
ATGCTATTCGCGAATGCGGCCTCCATTCGAGATGTGATACTCTTCCCAGCAATGAAGCAGAAATAA

Upstream 100 bases:

>100_bases
GGCGACTTAGATCGCTTTATTGAGGCCAGTTTAAAAGCCGGATTATAAATCCTCTGGGGGCTTCCCCCGACTAAAAAATA
CTAAAAATTAGGTAACCAAA

Downstream 100 bases:

>100_bases
TTCTCTCTATCCCCAGCATCCTGCTGGGGATAGCCTCTCTCACAGGATCTTTAATATGAAAAATCGTCGACTCAATTTCA
AACTGTTTTTCCTCATCATT

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 501; Mature: 500

Protein sequence:

>501_residues
MSEQTQELDLNGEMLVRREKLAALRAKGNPFPNTFRRDALAQDLHANYDALEGEALKAQEVEVKVAGRIMTRRAMGKATF
ITLQDMSGRIQLYVARDNLPEGIYADDVGQWDLGDIIGVKGTLFKTKTNELTVRCTEVQLLTKALRPLPDKFHGLSDQET
RYRQRYLDLIANEDSRRTFIIRSKVIAGIREYFLSKGFMEVETPMLQIIPGGASARPFVTHHNALDVDMYLRIAPELYLK
RLVVGGFERVFELNRNFRNEGVSVRHNPEFTMLEYYQAYADYHDLMDNTEELLRKLAMDILGTTIVPYGELEFDFGKPFE
RITMHDAILKYGAEKGIVKEDLYDFDRAVAVAKKLGIEVQKSWGLGSLVNVIFEEVAEHHLIQPTFLMAHPAEISPLARR
NDENPEVTDRFELFIGGREIGNGFSELNDAEDQNERFDAQVAAKEAGDDEAMFKDEDFVTALEHGLPPTAGEGLGIDRLA
MLFANAASIRDVILFPAMKQK

Sequences:

>Translated_501_residues
MSEQTQELDLNGEMLVRREKLAALRAKGNPFPNTFRRDALAQDLHANYDALEGEALKAQEVEVKVAGRIMTRRAMGKATF
ITLQDMSGRIQLYVARDNLPEGIYADDVGQWDLGDIIGVKGTLFKTKTNELTVRCTEVQLLTKALRPLPDKFHGLSDQET
RYRQRYLDLIANEDSRRTFIIRSKVIAGIREYFLSKGFMEVETPMLQIIPGGASARPFVTHHNALDVDMYLRIAPELYLK
RLVVGGFERVFELNRNFRNEGVSVRHNPEFTMLEYYQAYADYHDLMDNTEELLRKLAMDILGTTIVPYGELEFDFGKPFE
RITMHDAILKYGAEKGIVKEDLYDFDRAVAVAKKLGIEVQKSWGLGSLVNVIFEEVAEHHLIQPTFLMAHPAEISPLARR
NDENPEVTDRFELFIGGREIGNGFSELNDAEDQNERFDAQVAAKEAGDDEAMFKDEDFVTALEHGLPPTAGEGLGIDRLA
MLFANAASIRDVILFPAMKQK
>Mature_500_residues
SEQTQELDLNGEMLVRREKLAALRAKGNPFPNTFRRDALAQDLHANYDALEGEALKAQEVEVKVAGRIMTRRAMGKATFI
TLQDMSGRIQLYVARDNLPEGIYADDVGQWDLGDIIGVKGTLFKTKTNELTVRCTEVQLLTKALRPLPDKFHGLSDQETR
YRQRYLDLIANEDSRRTFIIRSKVIAGIREYFLSKGFMEVETPMLQIIPGGASARPFVTHHNALDVDMYLRIAPELYLKR
LVVGGFERVFELNRNFRNEGVSVRHNPEFTMLEYYQAYADYHDLMDNTEELLRKLAMDILGTTIVPYGELEFDFGKPFER
ITMHDAILKYGAEKGIVKEDLYDFDRAVAVAKKLGIEVQKSWGLGSLVNVIFEEVAEHHLIQPTFLMAHPAEISPLARRN
DENPEVTDRFELFIGGREIGNGFSELNDAEDQNERFDAQVAAKEAGDDEAMFKDEDFVTALEHGLPPTAGEGLGIDRLAM
LFANAASIRDVILFPAMKQK

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=513, Percent_Identity=43.2748538011696, Blast_Score=397, Evalue=1e-110,
Organism=Homo sapiens, GI194272210, Length=513, Percent_Identity=43.2748538011696, Blast_Score=396, Evalue=1e-110,
Organism=Homo sapiens, GI45439306, Length=467, Percent_Identity=23.7687366167024, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI40789249, Length=265, Percent_Identity=26.0377358490566, Blast_Score=83, Evalue=5e-16,
Organism=Escherichia coli, GI1790571, Length=504, Percent_Identity=69.8412698412698, Blast_Score=742, Evalue=0.0,
Organism=Escherichia coli, GI1789256, Length=504, Percent_Identity=69.6428571428571, Blast_Score=738, Evalue=0.0,
Organism=Escherichia coli, GI87082379, Length=327, Percent_Identity=31.4984709480122, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1788173, Length=282, Percent_Identity=25.177304964539, Blast_Score=86, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17535925, Length=500, Percent_Identity=42.2, Blast_Score=367, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17535927, Length=500, Percent_Identity=42.2, Blast_Score=367, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI71994340, Length=500, Percent_Identity=42.2, Blast_Score=366, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17551876, Length=486, Percent_Identity=24.6913580246914, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI32566633, Length=284, Percent_Identity=23.943661971831, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320242, Length=516, Percent_Identity=41.8604651162791, Blast_Score=386, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6324256, Length=474, Percent_Identity=29.535864978903, Blast_Score=180, Evalue=5e-46,
Organism=Saccharomyces cerevisiae, GI6323011, Length=481, Percent_Identity=25.7796257796258, Blast_Score=109, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24640851, Length=485, Percent_Identity=42.8865979381443, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24640849, Length=485, Percent_Identity=42.8865979381443, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI17136276, Length=461, Percent_Identity=23.644251626898, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24584738, Length=272, Percent_Identity=30.1470588235294, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 56760; Mature: 56629

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQTQELDLNGEMLVRREKLAALRAKGNPFPNTFRRDALAQDLHANYDALEGEALKAQE
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHH
VEVKVAGRIMTRRAMGKATFITLQDMSGRIQLYVARDNLPEGIYADDVGQWDLGDIIGVK
EEEHHHHHHHHHHHCCCEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
GTLFKTKTNELTVRCTEVQLLTKALRPLPDKFHGLSDQETRYRQRYLDLIANEDSRRTFI
CEEEEECCCCEEEEEHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEE
IRSKVIAGIREYFLSKGFMEVETPMLQIIPGGASARPFVTHHNALDVDMYLRIAPELYLK
EHHHHHHHHHHHHHHCCCHHHCCCEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHH
RLVVGGFERVFELNRNFRNEGVSVRHNPEFTMLEYYQAYADYHDLMDNTEELLRKLAMDI
HHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LGTTIVPYGELEFDFGKPFERITMHDAILKYGAEKGIVKEDLYDFDRAVAVAKKLGIEVQ
HCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEE
KSWGLGSLVNVIFEEVAEHHLIQPTFLMAHPAEISPLARRNDENPEVTDRFELFIGGREI
ECCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHCCCCCCCHHCEEEEEECCHHH
GNGFSELNDAEDQNERFDAQVAAKEAGDDEAMFKDEDFVTALEHGLPPTAGEGLGIDRLA
CCCHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHH
MLFANAASIRDVILFPAMKQK
HHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SEQTQELDLNGEMLVRREKLAALRAKGNPFPNTFRRDALAQDLHANYDALEGEALKAQE
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHH
VEVKVAGRIMTRRAMGKATFITLQDMSGRIQLYVARDNLPEGIYADDVGQWDLGDIIGVK
EEEHHHHHHHHHHHCCCEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
GTLFKTKTNELTVRCTEVQLLTKALRPLPDKFHGLSDQETRYRQRYLDLIANEDSRRTFI
CEEEEECCCCEEEEEHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEE
IRSKVIAGIREYFLSKGFMEVETPMLQIIPGGASARPFVTHHNALDVDMYLRIAPELYLK
EHHHHHHHHHHHHHHCCCHHHCCCEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHH
RLVVGGFERVFELNRNFRNEGVSVRHNPEFTMLEYYQAYADYHDLMDNTEELLRKLAMDI
HHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LGTTIVPYGELEFDFGKPFERITMHDAILKYGAEKGIVKEDLYDFDRAVAVAKKLGIEVQ
HCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEE
KSWGLGSLVNVIFEEVAEHHLIQPTFLMAHPAEISPLARRNDENPEVTDRFELFIGGREI
ECCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHCCCCCCCHHCEEEEEECCHHH
GNGFSELNDAEDQNERFDAQVAAKEAGDDEAMFKDEDFVTALEHGLPPTAGEGLGIDRLA
CCCHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHH
MLFANAASIRDVILFPAMKQK
HHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA