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The map label for this gene is metE

Identifier: 30024031

GI number: 30024031

Start: 1282597

End: 1284870

Strand: Reverse

Name: metE

Synonym: L0100

Alternate gene names: 30024031

Gene position: 1284870-1282597 (Counterclockwise)

Preceding gene: 15673238

Following gene: 15673236

Centisome position: 54.32

GC content: 32.67

Gene sequence:

>2274_bases
ATGAAAAAATCAATTATTGCTTTCCCACGTATCGGTTCAAACCGTGAATTAAAATTTGCTCTTGAAAAATATTTTCGTAA
AGAAATTACAGAAGAAGAATTACAAACAGTAGCCAAAGAAATTCGGTTGGAAAATTGGAAAAGTCAAAAAGAAGCTGGAA
TTGACTCACCTATTTCCAATGACTTTTCTTTCTATGACCAAACCTTAGATCTATCAATTGCTCTTGGTGCAATTCCAGGG
TCTTATAAAAATTTAGAATTAAGTGAACTAGATACTTTATTTGCATTAGCGCGTGGTTTCCAAGATGAACAAAATGACGT
AAAAGCAAGACCAATGAAAAAATGGTTTAATACAAACTATCATTATTTAGTTCCAGAAATTAACAAAGATACAATTATTA
AAGCAAATTTTTCAAAATTATTAAATGAATATAAGGAAGCAAAATCTGCTGGATTTGAAACCAGACCAACAATTATTGGC
CCATATACATTTTTAGTTTTAGCAGATTACAAGTCAGGAGCCACGAAAGATACAGTATTATCTGATGTTATTGTAGCTTT
TCAAACACTATTAAAAGAACTTAATCAACTAGGAGTAGAATGGTTACAGATTGAAGAACCAGCACTTGTTTTAGACCAAA
CTGAAGAAGAAAAAAAATTATTTGTGAGTATTTATGAAGAACTACTTAAGAGTAAAAACAACCTTAATATACTTCTTCAA
ACTTATTTTGGAGATGTTCGTGATAGCTATAAAGAAATAATAAAGCTTGATTTTGATGGTATTGGTCTTGATTTTATAGA
AGGAAGAGATTCTTTAGCTCTTATTCAAAAATACGGTTTTCCCAAAAAGAAAATACTTTTTGCAGGATTAGTAAATGGAA
AAAATATTTGGCGTAATAATTATCAAAAGACACTTGAGCTACTAACCGCTTTAGATGAAGTAGCAGATATCGTAATTAAT
ACAAGCTGCTCTTTACAACACGTTCCAGTAACAATTAAAAACGAAACTAAATTATCAAAAGAAATCCTTAAACACTTTTC
ATTTGCCACAGAAAAATTAAATGAAATTGATGAAATTTCCGATATTTATTTTAATAAAAACACTTCTTTTTTGAAGAAGA
ATGTCGAACTTTTTGAAAATGAAAGAGTTCGTGAAAATTCTAAACTTAAGCAAAAACTTGATAAATTAACAGAAAAAGAC
TTTACTAGACAACCTTCACTAGTCGAAAGACGAGCAAGTCAATCTCAATCGCTTAATTTACCAGTTTTACCAACCACAAC
CATCGGTTCATTTCCTCAAACGGCAGAAGTGAGAAAAGTTCGGCTACAAAATAAGCGAGGAGAATTAAGACAAGCAGATT
ATGACACATTCTTAGAAGAAAAAATAAAAGAATGTCTTAAACTCCAAGAAGATATTGGATTAGACGTTTTAGTTCATGGT
GAATTTGAACGAAATGACATGGTAGAGTATTTTGGAGAAAAGTTAGAAGGTTATGTTTTTACTCAAAAAGCATGGGTTCA
ATCTTATGGGACACGTTGTGTCAAACCTCCAATCGTGTGGACTGATGTCACTAGACCGGAAGCTATGACCGTCCGTTGGT
CAGCCTATGCTCAATCTCAAACATCTAAACCAGTAAAAGGAATGCTGACAGGTCCTGTAACGATTCTAAATTGGTCTTTT
CCGCGAGAAGATATTTCGCTAAAAGCAAGTACTTTACAATTGGCATTAGCTGTTCAAGAAGAAGTTCTTGACTTAGAGAA
AGCTGGCATTAAGATTATTCAAATTGATGAGGCAGCTTTGAGAGAAAAGTTACCTCTTCGCAAAAGTGATTGGTATAAAG
AATATCTAGATTGGGCAATTCCTGCCTTTCGTTTAGTTCACTCTAAAGTAAAACCGGAAACACAAATTCACACGCATATG
TGTTATTCTGAATTTGAAGATATTATTCCATCCATTGACGCAATGGATGCTGATGTAATTTCTTTTGAAGCAAGTAGAAG
TGAGCTATCAATTATCGATGCATTGAAAGCTCACCATTTTAAAACTTTGGTTGGACCTGGAGTCTATGATATTCATTCTC
CACGAATACCTAGCGTAAAAGAAATACAAAATCAATTAGAAAAAATATTGGACAAATTACCAATTGAACAAGTTTGGGTA
AACCCTGATTGTGGTCTTAAAACACGTGGTAATGCCGAAACCATTCCTAGTTTAAGACACTTAGTTGAGGCAACTAGGGT
GTTAAGAAAGGAAAAGGTTGTATATGACAAGTAA

Upstream 100 bases:

>100_bases
TCATATAGTTTAAAACTATAGGTCATCATATTAAAAATGTATTATTAAAGTTTTATTAAATTTGATAAGATAGACCTATT
AAAAATTGGAGACTATGACT

Downstream 100 bases:

>100_bases
TTCCAAAATTCTTTCTTTTGAAGTTTTTCCACCTACAACTCAAATTGGAAGTACCAACTTGGTAAAGACCTTGGATAGCC
TAAGAACTCTCTCGCCAGAT

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme

Number of amino acids: Translated: 757; Mature: 757

Protein sequence:

>757_residues
MKKSIIAFPRIGSNRELKFALEKYFRKEITEEELQTVAKEIRLENWKSQKEAGIDSPISNDFSFYDQTLDLSIALGAIPG
SYKNLELSELDTLFALARGFQDEQNDVKARPMKKWFNTNYHYLVPEINKDTIIKANFSKLLNEYKEAKSAGFETRPTIIG
PYTFLVLADYKSGATKDTVLSDVIVAFQTLLKELNQLGVEWLQIEEPALVLDQTEEEKKLFVSIYEELLKSKNNLNILLQ
TYFGDVRDSYKEIIKLDFDGIGLDFIEGRDSLALIQKYGFPKKKILFAGLVNGKNIWRNNYQKTLELLTALDEVADIVIN
TSCSLQHVPVTIKNETKLSKEILKHFSFATEKLNEIDEISDIYFNKNTSFLKKNVELFENERVRENSKLKQKLDKLTEKD
FTRQPSLVERRASQSQSLNLPVLPTTTIGSFPQTAEVRKVRLQNKRGELRQADYDTFLEEKIKECLKLQEDIGLDVLVHG
EFERNDMVEYFGEKLEGYVFTQKAWVQSYGTRCVKPPIVWTDVTRPEAMTVRWSAYAQSQTSKPVKGMLTGPVTILNWSF
PREDISLKASTLQLALAVQEEVLDLEKAGIKIIQIDEAALREKLPLRKSDWYKEYLDWAIPAFRLVHSKVKPETQIHTHM
CYSEFEDIIPSIDAMDADVISFEASRSELSIIDALKAHHFKTLVGPGVYDIHSPRIPSVKEIQNQLEKILDKLPIEQVWV
NPDCGLKTRGNAETIPSLRHLVEATRVLRKEKVVYDK

Sequences:

>Translated_757_residues
MKKSIIAFPRIGSNRELKFALEKYFRKEITEEELQTVAKEIRLENWKSQKEAGIDSPISNDFSFYDQTLDLSIALGAIPG
SYKNLELSELDTLFALARGFQDEQNDVKARPMKKWFNTNYHYLVPEINKDTIIKANFSKLLNEYKEAKSAGFETRPTIIG
PYTFLVLADYKSGATKDTVLSDVIVAFQTLLKELNQLGVEWLQIEEPALVLDQTEEEKKLFVSIYEELLKSKNNLNILLQ
TYFGDVRDSYKEIIKLDFDGIGLDFIEGRDSLALIQKYGFPKKKILFAGLVNGKNIWRNNYQKTLELLTALDEVADIVIN
TSCSLQHVPVTIKNETKLSKEILKHFSFATEKLNEIDEISDIYFNKNTSFLKKNVELFENERVRENSKLKQKLDKLTEKD
FTRQPSLVERRASQSQSLNLPVLPTTTIGSFPQTAEVRKVRLQNKRGELRQADYDTFLEEKIKECLKLQEDIGLDVLVHG
EFERNDMVEYFGEKLEGYVFTQKAWVQSYGTRCVKPPIVWTDVTRPEAMTVRWSAYAQSQTSKPVKGMLTGPVTILNWSF
PREDISLKASTLQLALAVQEEVLDLEKAGIKIIQIDEAALREKLPLRKSDWYKEYLDWAIPAFRLVHSKVKPETQIHTHM
CYSEFEDIIPSIDAMDADVISFEASRSELSIIDALKAHHFKTLVGPGVYDIHSPRIPSVKEIQNQLEKILDKLPIEQVWV
NPDCGLKTRGNAETIPSLRHLVEATRVLRKEKVVYDK
>Mature_757_residues
MKKSIIAFPRIGSNRELKFALEKYFRKEITEEELQTVAKEIRLENWKSQKEAGIDSPISNDFSFYDQTLDLSIALGAIPG
SYKNLELSELDTLFALARGFQDEQNDVKARPMKKWFNTNYHYLVPEINKDTIIKANFSKLLNEYKEAKSAGFETRPTIIG
PYTFLVLADYKSGATKDTVLSDVIVAFQTLLKELNQLGVEWLQIEEPALVLDQTEEEKKLFVSIYEELLKSKNNLNILLQ
TYFGDVRDSYKEIIKLDFDGIGLDFIEGRDSLALIQKYGFPKKKILFAGLVNGKNIWRNNYQKTLELLTALDEVADIVIN
TSCSLQHVPVTIKNETKLSKEILKHFSFATEKLNEIDEISDIYFNKNTSFLKKNVELFENERVRENSKLKQKLDKLTEKD
FTRQPSLVERRASQSQSLNLPVLPTTTIGSFPQTAEVRKVRLQNKRGELRQADYDTFLEEKIKECLKLQEDIGLDVLVHG
EFERNDMVEYFGEKLEGYVFTQKAWVQSYGTRCVKPPIVWTDVTRPEAMTVRWSAYAQSQTSKPVKGMLTGPVTILNWSF
PREDISLKASTLQLALAVQEEVLDLEKAGIKIIQIDEAALREKLPLRKSDWYKEYLDWAIPAFRLVHSKVKPETQIHTHM
CYSEFEDIIPSIDAMDADVISFEASRSELSIIDALKAHHFKTLVGPGVYDIHSPRIPSVKEIQNQLEKILDKLPIEQVWV
NPDCGLKTRGNAETIPSLRHLVEATRVLRKEKVVYDK

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family

Homologues:

Organism=Escherichia coli, GI2367304, Length=751, Percent_Identity=45.4061251664447, Blast_Score=665, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=776, Percent_Identity=44.201030927835, Blast_Score=621, Evalue=1e-178,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METE_LACLA (Q9CG55)

Other databases:

- EMBL:   AE005176
- PIR:   G86781
- RefSeq:   NP_267411.2
- ProteinModelPortal:   Q9CG55
- SMR:   Q9CG55
- GeneID:   1114904
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0100
- NMPDR:   fig|272623.1.peg.1290
- HOGENOM:   HBG287495
- OMA:   RFGWVQS
- ProtClustDB:   PRK05222
- BioCyc:   LLAC272623:L0100-MONOMER
- BRENDA:   2.1.1.14
- HAMAP:   MF_00172
- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629
- PIRSF:   PIRSF000382
- TIGRFAMs:   TIGR01371

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2

EC number: =2.1.1.14

Molecular weight: Translated: 87017; Mature: 87017

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSIIAFPRIGSNRELKFALEKYFRKEITEEELQTVAKEIRLENWKSQKEAGIDSPISN
CCCCEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHCCCCCCCCC
DFSFYDQTLDLSIALGAIPGSYKNLELSELDTLFALARGFQDEQNDVKARPMKKWFNTNY
CHHHHHHHEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCC
HYLVPEINKDTIIKANFSKLLNEYKEAKSAGFETRPTIIGPYTFLVLADYKSGATKDTVL
EEEEEECCCCCEEECHHHHHHHHHHHHHHCCCCCCCCEECCEEEEEEEECCCCCCHHHHH
SDVIVAFQTLLKELNQLGVEWLQIEEPALVLDQTEEEKKLFVSIYEELLKSKNNLNILLQ
HHHHHHHHHHHHHHHHCCCEEEEECCCEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEH
TYFGDVRDSYKEIIKLDFDGIGLDFIEGRDSLALIQKYGFPKKKILFAGLVNGKNIWRNN
HHHHHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHCCCCHHHEEEEEECCCCHHHHHH
YQKTLELLTALDEVADIVINTSCSLQHVPVTIKNETKLSKEILKHFSFATEKLNEIDEIS
HHHHHHHHHHHHHHHHHHCCCCCCEEECCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
DIYFNKNTSFLKKNVELFENERVRENSKLKQKLDKLTEKDFTRQPSLVERRASQSQSLNL
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCC
PVLPTTTIGSFPQTAEVRKVRLQNKRGELRQADYDTFLEEKIKECLKLQEDIGLDVLVHG
CCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
EFERNDMVEYFGEKLEGYVFTQKAWVQSYGTRCVKPPIVWTDVTRPEAMTVRWSAYAQSQ
CCCCCHHHHHHHHHHCCEEEHHHHHHHHHCCCCCCCCEEEECCCCCCEEEEEEHHHHHCC
TSKPVKGMLTGPVTILNWSFPREDISLKASTLQLALAVQEEVLDLEKAGIKIIQIDEAAL
CCCCCCCEECCCEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCEEEEEECHHHH
REKLPLRKSDWYKEYLDWAIPAFRLVHSKVKPETQIHTHMCYSEFEDIIPSIDAMDADVI
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHH
SFEASRSELSIIDALKAHHFKTLVGPGVYDIHSPRIPSVKEIQNQLEKILDKLPIEQVWV
EEECCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHCCHHHEEE
NPDCGLKTRGNAETIPSLRHLVEATRVLRKEKVVYDK
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKSIIAFPRIGSNRELKFALEKYFRKEITEEELQTVAKEIRLENWKSQKEAGIDSPISN
CCCCEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHCCCCCCCCC
DFSFYDQTLDLSIALGAIPGSYKNLELSELDTLFALARGFQDEQNDVKARPMKKWFNTNY
CHHHHHHHEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCC
HYLVPEINKDTIIKANFSKLLNEYKEAKSAGFETRPTIIGPYTFLVLADYKSGATKDTVL
EEEEEECCCCCEEECHHHHHHHHHHHHHHCCCCCCCCEECCEEEEEEEECCCCCCHHHHH
SDVIVAFQTLLKELNQLGVEWLQIEEPALVLDQTEEEKKLFVSIYEELLKSKNNLNILLQ
HHHHHHHHHHHHHHHHCCCEEEEECCCEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEH
TYFGDVRDSYKEIIKLDFDGIGLDFIEGRDSLALIQKYGFPKKKILFAGLVNGKNIWRNN
HHHHHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHCCCCHHHEEEEEECCCCHHHHHH
YQKTLELLTALDEVADIVINTSCSLQHVPVTIKNETKLSKEILKHFSFATEKLNEIDEIS
HHHHHHHHHHHHHHHHHHCCCCCCEEECCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
DIYFNKNTSFLKKNVELFENERVRENSKLKQKLDKLTEKDFTRQPSLVERRASQSQSLNL
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCC
PVLPTTTIGSFPQTAEVRKVRLQNKRGELRQADYDTFLEEKIKECLKLQEDIGLDVLVHG
CCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
EFERNDMVEYFGEKLEGYVFTQKAWVQSYGTRCVKPPIVWTDVTRPEAMTVRWSAYAQSQ
CCCCCHHHHHHHHHHCCEEEHHHHHHHHHCCCCCCCCEEEECCCCCCEEEEEEHHHHHCC
TSKPVKGMLTGPVTILNWSFPREDISLKASTLQLALAVQEEVLDLEKAGIKIIQIDEAAL
CCCCCCCEECCCEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCEEEEEECHHHH
REKLPLRKSDWYKEYLDWAIPAFRLVHSKVKPETQIHTHMCYSEFEDIIPSIDAMDADVI
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHH
SFEASRSELSIIDALKAHHFKTLVGPGVYDIHSPRIPSVKEIQNQLEKILDKLPIEQVWV
EEECCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHCCHHHEEE
NPDCGLKTRGNAETIPSLRHLVEATRVLRKEKVVYDK
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471