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The map label for this gene is metF

Identifier: 15673236

GI number: 15673236

Start: 1281756

End: 1282607

Strand: Reverse

Name: metF

Synonym: L0099

Alternate gene names: 15673236

Gene position: 1282607-1281756 (Counterclockwise)

Preceding gene: 30024031

Following gene: 15673235

Centisome position: 54.22

GC content: 33.33

Gene sequence:

>852_bases
ATGACAAGTAATTCCAAAATTCTTTCTTTTGAAGTTTTTCCACCTACAACTCAAATTGGAAGTACCAACTTGGTAAAGAC
CTTGGATAGCCTAAGAACTCTCTCGCCAGATTTTATCAGTGTAACTTGTAGTAACAATAATTATGATAATATTGGAGATA
CAACTATAAAGTTTGCTGATTATGTAAACAATACACTAGATATTCCAGCGGTTGCTCATTTACCTGCCGCTTATTTAGAT
AAAGCTCAAGTGATCGAAATTTTGGAACGGTTAAAAGATAAACAAATCAAAAAAATTCTTGCTTTAAGAGGTGATATCAG
CGATGAACCGATGAAAGATGATTTTAAATTTGCAAGTGATTTGGTTAAATTTATCAAAGATTATGATGATAGTTTTGAAG
TTTTAGGTGCTTGCTACCCCGATATTCATCCCGAATCAGTAAATCGAGTGAGTGATTTTCATTATCTGAAAGAAAAAGTA
GATGCTGGTTGTGACAGATTAATCACGCAACTATTTTTTGATAATGATAGTTTCTATGATTTTCAAGAACGATGCGCAAT
TGCTGAGATAAATACTCCGATATTCGCCGGAATAATGCCAGTAATCAATCGAAATCAAATTCTTCGTCTATTAAAAAATT
GTAATACGCCATTACCAGCAAAATTCATTAGAATACTCGAAAAATATGAACATAATCTTATCGCTTTAAGGGATGCTGGA
ATTGCTTACGCCATCGATCAAATCGTTGATTTAGTAACAGAGGATGTTGCTGGAATTCACCTCTATACGATGAATAATGC
AAATACGGCACACTCCATCCATGCTTCAATTTCTTCTTTATTTACCTTTTGA

Upstream 100 bases:

>100_bases
CCTGATTGTGGTCTTAAAACACGTGGTAATGCCGAAACCATTCCTAGTTTAAGACACTTAGTTGAGGCAACTAGGGTGTT
AAGAAAGGAAAAGGTTGTAT

Downstream 100 bases:

>100_bases
TACATAATAAAAGTTATGTAAAATGAAATTTTGAAATAAAATTAAGAAAAAATAGATAAATAATTGACAAATCAAAAAAT
TTTAATTATACTAACTAATA

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MTSNSKILSFEVFPPTTQIGSTNLVKTLDSLRTLSPDFISVTCSNNNYDNIGDTTIKFADYVNNTLDIPAVAHLPAAYLD
KAQVIEILERLKDKQIKKILALRGDISDEPMKDDFKFASDLVKFIKDYDDSFEVLGACYPDIHPESVNRVSDFHYLKEKV
DAGCDRLITQLFFDNDSFYDFQERCAIAEINTPIFAGIMPVINRNQILRLLKNCNTPLPAKFIRILEKYEHNLIALRDAG
IAYAIDQIVDLVTEDVAGIHLYTMNNANTAHSIHASISSLFTF

Sequences:

>Translated_283_residues
MTSNSKILSFEVFPPTTQIGSTNLVKTLDSLRTLSPDFISVTCSNNNYDNIGDTTIKFADYVNNTLDIPAVAHLPAAYLD
KAQVIEILERLKDKQIKKILALRGDISDEPMKDDFKFASDLVKFIKDYDDSFEVLGACYPDIHPESVNRVSDFHYLKEKV
DAGCDRLITQLFFDNDSFYDFQERCAIAEINTPIFAGIMPVINRNQILRLLKNCNTPLPAKFIRILEKYEHNLIALRDAG
IAYAIDQIVDLVTEDVAGIHLYTMNNANTAHSIHASISSLFTF
>Mature_282_residues
TSNSKILSFEVFPPTTQIGSTNLVKTLDSLRTLSPDFISVTCSNNNYDNIGDTTIKFADYVNNTLDIPAVAHLPAAYLDK
AQVIEILERLKDKQIKKILALRGDISDEPMKDDFKFASDLVKFIKDYDDSFEVLGACYPDIHPESVNRVSDFHYLKEKVD
AGCDRLITQLFFDNDSFYDFQERCAIAEINTPIFAGIMPVINRNQILRLLKNCNTPLPAKFIRILEKYEHNLIALRDAGI
AYAIDQIVDLVTEDVAGIHLYTMNNANTAHSIHASISSLFTF

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=272, Percent_Identity=31.9852941176471, Blast_Score=142, Evalue=4e-34,
Organism=Escherichia coli, GI1790377, Length=266, Percent_Identity=33.0827067669173, Blast_Score=144, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI25147441, Length=283, Percent_Identity=29.3286219081272, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI25147436, Length=283, Percent_Identity=29.3286219081272, Blast_Score=122, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6325234, Length=284, Percent_Identity=32.3943661971831, Blast_Score=134, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6321313, Length=274, Percent_Identity=29.5620437956204, Blast_Score=113, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 31911; Mature: 31779

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSNSKILSFEVFPPTTQIGSTNLVKTLDSLRTLSPDFISVTCSNNNYDNIGDTTIKFAD
CCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHH
YVNNTLDIPAVAHLPAAYLDKAQVIEILERLKDKQIKKILALRGDISDEPMKDDFKFASD
HHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
LVKFIKDYDDSFEVLGACYPDIHPESVNRVSDFHYLKEKVDAGCDRLITQLFFDNDSFYD
HHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
FQERCAIAEINTPIFAGIMPVINRNQILRLLKNCNTPLPAKFIRILEKYEHNLIALRDAG
HHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCC
IAYAIDQIVDLVTEDVAGIHLYTMNNANTAHSIHASISSLFTF
HHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSNSKILSFEVFPPTTQIGSTNLVKTLDSLRTLSPDFISVTCSNNNYDNIGDTTIKFAD
CCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHH
YVNNTLDIPAVAHLPAAYLDKAQVIEILERLKDKQIKKILALRGDISDEPMKDDFKFASD
HHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
LVKFIKDYDDSFEVLGACYPDIHPESVNRVSDFHYLKEKVDAGCDRLITQLFFDNDSFYD
HHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
FQERCAIAEINTPIFAGIMPVINRNQILRLLKNCNTPLPAKFIRILEKYEHNLIALRDAG
HHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCC
IAYAIDQIVDLVTEDVAGIHLYTMNNANTAHSIHASISSLFTF
HHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]