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The map label for this gene is pacB

Identifier: 15673817

GI number: 15673817

Start: 1903070

End: 1904071

Strand: Reverse

Name: pacB

Synonym: L86424

Alternate gene names: NA

Gene position: 1904071-1903070 (Counterclockwise)

Preceding gene: 15673818

Following gene: 15673816

Centisome position: 80.49

GC content: 32.73

Gene sequence:

>1002_bases
ATGATTGGATGTAGTAGTTTTACACTAGAGAGTCAAGATAATAAACATTTTCTTTCTCGTACAATGGATTTTCAAATAGA
AATGGCGGAACAAATTCTTTTTATTCCAAGAAACAAGGAGATAGGATTTGCCCACTCTAATGAAGAGACAATTGAAACAT
CCTATGCTTGTTTAGGGATGGGTGCGATGGAAGAGGGCCATCCAGTACTCTTTGATGGAATCAACGAAAAAGGGTTAATG
GGTGCGACTTTGTATTTCCCAGGCTATGCTGACTATAGTGAGAATATCAAAAAAAATCAAAAGGGAATTTCACCTGATAT
GGTTATTCCAACGGTCCTCACTCAAGCGAGTAATTTAGAAGAAATCATTGATTTATTTGATAAAAAATTTGTTATTATTA
ATGATACTAATCCAACCTTAGGTCTTACGCCTCCTTTACATTTTATTTTTTCTGATTCTAGTGGTCAAAGTTTGATTATT
GAACCAAGGCAAGGTGGACTTTCAATTATAAAAGATTCAATTGGAGTGATGACAAATAGTCCAGATTACCAATGGCACGA
AACTAATTTAAGAAATTATCTTTCTTTTACACCTAATCAAAAAGAAAGTATCGAACTATTAGGAAAGACATTAAAACCAT
TTAGTCAAGGTTCAGGTACTTTTGGATTTCCAGGAGATTTTACACCTCCTTCAAGATTTGTTAGAACGGCTTATTTAAAA
AACTACGCTGAGAAACCAAGTAATGAATTAGCCGCTATTACGCTTTGTCATCATATATTAGAATCTGTAATTATTCCAAA
AGGAATTGTTATAACTGAGCATGGAGCAAGTGATTTCACTTGTTATTCGGCATATATGTGCTCAGAAACTCTAAGTTATT
ATTTTTCAACTTATGGTAATCAAAGAATCAGGAAAATAAGTTTGTCAGAATCCTTGAAAAATGAGAAGGAATTTAAAAAC
TTTCCTATTGTCAATGAGGAAGATATTTTAGAGTTAAATTGA

Upstream 100 bases:

>100_bases
AAAGTATTTATTTAAATTATAAATTATAAAAAAGTGATTTTGAACTCTTTTGCTAATTGATGTATAATTAAGTTAATAGA
ATACATTGGAGGTACAAAAG

Downstream 100 bases:

>100_bases
TAAATATTTAATCGAGTAAATAGGAGGGATATTCATTAAGTTCTTTGAATTCAAGAAGTATTTTTTAAATATTAGTTTTA
AGTATAGCAATCCTAAAAAG

Product: penicillin acylase

Products: NA

Alternate protein names: Penicillin V amidase; PVA [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MIGCSSFTLESQDNKHFLSRTMDFQIEMAEQILFIPRNKEIGFAHSNEETIETSYACLGMGAMEEGHPVLFDGINEKGLM
GATLYFPGYADYSENIKKNQKGISPDMVIPTVLTQASNLEEIIDLFDKKFVIINDTNPTLGLTPPLHFIFSDSSGQSLII
EPRQGGLSIIKDSIGVMTNSPDYQWHETNLRNYLSFTPNQKESIELLGKTLKPFSQGSGTFGFPGDFTPPSRFVRTAYLK
NYAEKPSNELAAITLCHHILESVIIPKGIVITEHGASDFTCYSAYMCSETLSYYFSTYGNQRIRKISLSESLKNEKEFKN
FPIVNEEDILELN

Sequences:

>Translated_333_residues
MIGCSSFTLESQDNKHFLSRTMDFQIEMAEQILFIPRNKEIGFAHSNEETIETSYACLGMGAMEEGHPVLFDGINEKGLM
GATLYFPGYADYSENIKKNQKGISPDMVIPTVLTQASNLEEIIDLFDKKFVIINDTNPTLGLTPPLHFIFSDSSGQSLII
EPRQGGLSIIKDSIGVMTNSPDYQWHETNLRNYLSFTPNQKESIELLGKTLKPFSQGSGTFGFPGDFTPPSRFVRTAYLK
NYAEKPSNELAAITLCHHILESVIIPKGIVITEHGASDFTCYSAYMCSETLSYYFSTYGNQRIRKISLSESLKNEKEFKN
FPIVNEEDILELN
>Mature_333_residues
MIGCSSFTLESQDNKHFLSRTMDFQIEMAEQILFIPRNKEIGFAHSNEETIETSYACLGMGAMEEGHPVLFDGINEKGLM
GATLYFPGYADYSENIKKNQKGISPDMVIPTVLTQASNLEEIIDLFDKKFVIINDTNPTLGLTPPLHFIFSDSSGQSLII
EPRQGGLSIIKDSIGVMTNSPDYQWHETNLRNYLSFTPNQKESIELLGKTLKPFSQGSGTFGFPGDFTPPSRFVRTAYLK
NYAEKPSNELAAITLCHHILESVIIPKGIVITEHGASDFTCYSAYMCSETLSYYFSTYGNQRIRKISLSESLKNEKEFKN
FPIVNEEDILELN

Specific function: The enzyme catalyzes the conversion of penicillin to 6- aminopenicillanate The precursor, furthermore, acts as a self- processing peptidase that cleaves off the propeptide. All peptidase activity is lost on conversion to the mature peptidase [H]

COG id: COG3049

COG function: function code M; Penicillin V acylase and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase C59 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003199 [H]

Pfam domain/function: PF02275 CBAH [H]

EC number: =3.5.1.11 [H]

Molecular weight: Translated: 37403; Mature: 37403

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00152 ATPASE_ALPHA_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGCSSFTLESQDNKHFLSRTMDFQIEMAEQILFIPRNKEIGFAHSNEETIETSYACLGM
CCCCCCEEECCCCCHHHHHHHHCCEEEECCEEEEEECCCCCCEECCCCHHHHHHHHHHCC
GAMEEGHPVLFDGINEKGLMGATLYFPGYADYSENIKKNQKGISPDMVIPTVLTQASNLE
CCCCCCCEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCHH
EIIDLFDKKFVIINDTNPTLGLTPPLHFIFSDSSGQSLIIEPRQGGLSIIKDSIGVMTNS
HHHHHHCCCEEEEECCCCCCCCCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHCCEECC
PDYQWHETNLRNYLSFTPNQKESIELLGKTLKPFSQGSGTFGFPGDFTPPSRFVRTAYLK
CCCCEECCCHHHHEECCCCCHHHHHHHHHHHCHHHCCCCCCCCCCCCCCHHHHHHHHHHH
NYAEKPSNELAAITLCHHILESVIIPKGIVITEHGASDFTCYSAYMCSETLSYYFSTYGN
HHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
QRIRKISLSESLKNEKEFKNFPIVNEEDILELN
CCEEEEEHHHHHHHHHHHCCCCCCCCCCCEECC
>Mature Secondary Structure
MIGCSSFTLESQDNKHFLSRTMDFQIEMAEQILFIPRNKEIGFAHSNEETIETSYACLGM
CCCCCCEEECCCCCHHHHHHHHCCEEEECCEEEEEECCCCCCEECCCCHHHHHHHHHHCC
GAMEEGHPVLFDGINEKGLMGATLYFPGYADYSENIKKNQKGISPDMVIPTVLTQASNLE
CCCCCCCEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCHH
EIIDLFDKKFVIINDTNPTLGLTPPLHFIFSDSSGQSLIIEPRQGGLSIIKDSIGVMTNS
HHHHHHCCCEEEEECCCCCCCCCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHCCEECC
PDYQWHETNLRNYLSFTPNQKESIELLGKTLKPFSQGSGTFGFPGDFTPPSRFVRTAYLK
CCCCEECCCHHHHEECCCCCHHHHHHHHHHHCHHHCCCCCCCCCCCCCCHHHHHHHHHHH
NYAEKPSNELAAITLCHHILESVIIPKGIVITEHGASDFTCYSAYMCSETLSYYFSTYGN
HHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
QRIRKISLSESLKNEKEFKNFPIVNEEDILELN
CCEEEEEHHHHHHHHHHHCCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3026906 [H]