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The map label for this gene is amtB

Identifier: 15673574

GI number: 15673574

Start: 1635861

End: 1637102

Strand: Reverse

Name: amtB

Synonym: L0236

Alternate gene names: 15673574

Gene position: 1637102-1635861 (Counterclockwise)

Preceding gene: 15673575

Following gene: 15673573

Centisome position: 69.2

GC content: 40.1

Gene sequence:

>1242_bases
ATGGACACAGGCTCAATTGCATTTATTTTAATTTGTGCGGCATTGGTTTTTTTGATGACGCCTGCACTAGCATTTTTTTA
TGGAGGGCTTGGACGCCGAAAAAATGTGTTAAACACAATGATGATGTCTCTCGCACCAATGGCTCTGGCTTCTATTTTAT
GGATAATTATTGGCTTCTCATTTTCATTTTCAGGAAGCAATAGTTGGATTGGTGATTTCCACCATTTGTTTATGAATGGT
GTGGATATGGCTAAAAATTCACTTTTCCCTGCTAATCATATTCCAGATGGCCTTTTTTCTGGTTTTCAAATGATGTTCTC
GATTATCACAGTAGCTCTAATTACGGGATCTGTTGTTGGAAGAATGAGATTTACACCAATTTTGATTTTTATGACTGCTT
GGTTAATTTTAGTTTATTATCCTTTGGCTCATATGGTTTGGGGCGGTGGATTTTTGGCTCAAATTCATGCGATTGACTTT
GCGGGTGGAGATGTTGTTCATATTTCAAGTGGGGTTACAGGACTTGGACTTGCGTTAGTACTTGGAAAACGTCGAGATTA
CGAACGTTTAGATTATCGTCCACATAATATTCCTTTTGTTGTTTTGGGCGCTGGTCTCCTATGGTTTGGTTGGTTCGGCT
TTAATGCTGGGTCAGCGCTTGCTGCCAATGGTGTGGCAATTAACGCTTTTATGACGACTAATACAGCGGCAGCAGCAGCA
ATGTTTTCATGGATGATTGTTGAAAAAATATTGATTGGTAAACCTTCAATTGTTGGTGCTTGTTCTGGTGCGGTAGTTGG
TTTGGTGGCCATCACTCCAGGCGCTGGTTTTGTTTCTTTATGGTCGTCATTGATTATTGGTCTTTTGGTCAGTCCTTTGT
CATACTTTATGATTTCTGTAGTAAAAAAGAAATTGGGATATGATGATGCTTTAGATGCTTTTGGTTGTCACGGTATTGGC
GGGATGTTTGGCGGAATTATGACAGGAATTTTTGCAACGCCGGCTTTGGCACCAGAAAAAGGCTACGCTGGTTTAATCTA
TGGCTCTGGGAAATTACTTTTGGCTAATGTGTCTGCGGTTGTCTTCACAGTTATTTTCACTGCTCTTGTGAGTTGGATCA
TTATTAAAGTAATTGCCTTGTTTATGCCAATTCGTGTATCTGACCGTGCTGAAGCTATTGGACTTGACGACAGTGAGCAC
GAAGAAACTGCTTATCCAACCTTCTTAGGTTTGGATTCTTAA

Upstream 100 bases:

>100_bases
TTGTTTAGAAAGTTGTGTTATATTTTATTACATGATTAAATTGTCAGAAAATATTGATAAATAATCTGTTTTAATCTCGA
ACTTTTAAAGGAGAAATTTT

Downstream 100 bases:

>100_bases
GTTTAATTTTACTGACAGATATTTTTTATATAAAATTGTGAGTTTGTCAGTAAAATCAATTTTGAATGATGTCTTTGAAG
TATACTGACAAGTCTGTCAG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MDTGSIAFILICAALVFLMTPALAFFYGGLGRRKNVLNTMMMSLAPMALASILWIIIGFSFSFSGSNSWIGDFHHLFMNG
VDMAKNSLFPANHIPDGLFSGFQMMFSIITVALITGSVVGRMRFTPILIFMTAWLILVYYPLAHMVWGGGFLAQIHAIDF
AGGDVVHISSGVTGLGLALVLGKRRDYERLDYRPHNIPFVVLGAGLLWFGWFGFNAGSALAANGVAINAFMTTNTAAAAA
MFSWMIVEKILIGKPSIVGACSGAVVGLVAITPGAGFVSLWSSLIIGLLVSPLSYFMISVVKKKLGYDDALDAFGCHGIG
GMFGGIMTGIFATPALAPEKGYAGLIYGSGKLLLANVSAVVFTVIFTALVSWIIIKVIALFMPIRVSDRAEAIGLDDSEH
EETAYPTFLGLDS

Sequences:

>Translated_413_residues
MDTGSIAFILICAALVFLMTPALAFFYGGLGRRKNVLNTMMMSLAPMALASILWIIIGFSFSFSGSNSWIGDFHHLFMNG
VDMAKNSLFPANHIPDGLFSGFQMMFSIITVALITGSVVGRMRFTPILIFMTAWLILVYYPLAHMVWGGGFLAQIHAIDF
AGGDVVHISSGVTGLGLALVLGKRRDYERLDYRPHNIPFVVLGAGLLWFGWFGFNAGSALAANGVAINAFMTTNTAAAAA
MFSWMIVEKILIGKPSIVGACSGAVVGLVAITPGAGFVSLWSSLIIGLLVSPLSYFMISVVKKKLGYDDALDAFGCHGIG
GMFGGIMTGIFATPALAPEKGYAGLIYGSGKLLLANVSAVVFTVIFTALVSWIIIKVIALFMPIRVSDRAEAIGLDDSEH
EETAYPTFLGLDS
>Mature_413_residues
MDTGSIAFILICAALVFLMTPALAFFYGGLGRRKNVLNTMMMSLAPMALASILWIIIGFSFSFSGSNSWIGDFHHLFMNG
VDMAKNSLFPANHIPDGLFSGFQMMFSIITVALITGSVVGRMRFTPILIFMTAWLILVYYPLAHMVWGGGFLAQIHAIDF
AGGDVVHISSGVTGLGLALVLGKRRDYERLDYRPHNIPFVVLGAGLLWFGWFGFNAGSALAANGVAINAFMTTNTAAAAA
MFSWMIVEKILIGKPSIVGACSGAVVGLVAITPGAGFVSLWSSLIIGLLVSPLSYFMISVVKKKLGYDDALDAFGCHGIG
GMFGGIMTGIFATPALAPEKGYAGLIYGSGKLLLANVSAVVFTVIFTALVSWIIIKVIALFMPIRVSDRAEAIGLDDSEH
EETAYPTFLGLDS

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=407, Percent_Identity=41.5233415233415, Blast_Score=307, Evalue=9e-85,
Organism=Caenorhabditis elegans, GI17550182, Length=438, Percent_Identity=27.1689497716895, Blast_Score=101, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17550180, Length=430, Percent_Identity=26.2790697674419, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI133931100, Length=409, Percent_Identity=24.9388753056235, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI32564042, Length=413, Percent_Identity=23.728813559322, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324187, Length=430, Percent_Identity=34.8837209302326, Blast_Score=224, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6325396, Length=416, Percent_Identity=32.6923076923077, Blast_Score=218, Evalue=9e-58,
Organism=Saccharomyces cerevisiae, GI6321559, Length=414, Percent_Identity=32.8502415458937, Blast_Score=215, Evalue=1e-56,
Organism=Drosophila melanogaster, GI161078314, Length=413, Percent_Identity=28.0871670702179, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 44020; Mature: 44020

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTGSIAFILICAALVFLMTPALAFFYGGLGRRKNVLNTMMMSLAPMALASILWIIIGFS
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FSFSGSNSWIGDFHHLFMNGVDMAKNSLFPANHIPDGLFSGFQMMFSIITVALITGSVVG
EEECCCCCCHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RMRFTPILIFMTAWLILVYYPLAHMVWGGGFLAQIHAIDFAGGDVVHISSGVTGLGLALV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEEEEECCCCEEEECCCCHHHHHHHH
LGKRRDYERLDYRPHNIPFVVLGAGLLWFGWFGFNAGSALAANGVAINAFMTTNTAAAAA
HCCCCCHHHHCCCCCCCCEEEEHHHHHHHHHHHCCCCCHHEECCEEEEEEEECCHHHHHH
MFSWMIVEKILIGKPSIVGACSGAVVGLVAITPGAGFVSLWSSLIIGLLVSPLSYFMISV
HHHHHHHHHHHHCCCCHHHHCCHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VKKKLGYDDALDAFGCHGIGGMFGGIMTGIFATPALAPEKGYAGLIYGSGKLLLANVSAV
HHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEEEHHHHHH
VFTVIFTALVSWIIIKVIALFMPIRVSDRAEAIGLDDSEHEETAYPTFLGLDS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEECCCC
>Mature Secondary Structure
MDTGSIAFILICAALVFLMTPALAFFYGGLGRRKNVLNTMMMSLAPMALASILWIIIGFS
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FSFSGSNSWIGDFHHLFMNGVDMAKNSLFPANHIPDGLFSGFQMMFSIITVALITGSVVG
EEECCCCCCHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RMRFTPILIFMTAWLILVYYPLAHMVWGGGFLAQIHAIDFAGGDVVHISSGVTGLGLALV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEEEEECCCCEEEECCCCHHHHHHHH
LGKRRDYERLDYRPHNIPFVVLGAGLLWFGWFGFNAGSALAANGVAINAFMTTNTAAAAA
HCCCCCHHHHCCCCCCCCEEEEHHHHHHHHHHHCCCCCHHEECCEEEEEEEECCHHHHHH
MFSWMIVEKILIGKPSIVGACSGAVVGLVAITPGAGFVSLWSSLIIGLLVSPLSYFMISV
HHHHHHHHHHHHCCCCHHHHCCHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VKKKLGYDDALDAFGCHGIGGMFGGIMTGIFATPALAPEKGYAGLIYGSGKLLLANVSAV
HHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEEEHHHHHH
VFTVIFTALVSWIIIKVIALFMPIRVSDRAEAIGLDDSEHEETAYPTFLGLDS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]