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The map label for this gene is trpE

Identifier: 15673452

GI number: 15673452

Start: 1500112

End: 1501482

Strand: Reverse

Name: trpE

Synonym: L0054

Alternate gene names: 15673452

Gene position: 1501482-1500112 (Counterclockwise)

Preceding gene: 15673453

Following gene: 15673451

Centisome position: 63.47

GC content: 37.35

Gene sequence:

>1371_bases
ATGAGAAAAATTAAAGAAATTTCAGCAGACACCATGACCCCGATTTCGGTTTATCTTCGCCTGAAAGGGAAAAATAAAGT
GATTTTAGAATCCATTCCCAGAGAAAATGACCAATCACGTTTTTCAATTATTGCGTTGAATCCTGTGAAGCACATCAAAT
TTACTGACGGAATTTTAAGCGTAAATGACGAAATCATTAGCGATGAAAATCCAATGGAATTTCTGGAAAAATTAGTTTGT
CAGCCAGAAAGTACTGACGAAAATTTAGACTTGCCTTTTACAAGTGGAGCGATTGGATATGCTGGATTTGATACCTATGG
TATTTTTGAAGGCATTCAACCTGAACTGAAAGATTCGATTGGAACACCAGATATGTATTTTATGTTATACGAATCAGCTC
TAATTTTTGACCACAAACGAGAAAAACTGATTTTTATTGAAGACAATACTTACAGTCAGCGTTCAGAAAAAGAGTTACAA
AACGCGCTGTCAGCAAATATTGAGTCATTATCTTTACTGACAGAAGCAGAAAATGAACTGACAGAATTAAGTAAACTGAA
TTTCGTCAGCAATATGTCTCAAGAACTTTTTGAAGAAAAAGTTGCTAAAGCAAAGGAACTTATCAGAAATGGTGACATGT
TCCAAGTTGTTTTATCACAACGTTTAACAGCCGATTTTACTGACAACCCTTTTAATTATTACAGAAAGCTTCGGGTTGAA
AATCCGTCATCGTATATGTACTTTATGGAATTCGATAATTTTCATGTCATTGGCAGCTCACCAGAGCGTTTGGTTGCAGT
TCATGGAAATCAAGTTTCAACCAATCCAATTGCCGGAACAAGAAAACGCGGGCAAACTGAATTTGAAGACCAAGCTCTTA
TTGAAGATTTAGAAAGTGATCCCAAAGAAGTCGCTGAACACAAAATGCTTGTTGATTTAGGACGAAATGATATTGGTAAA
ATTTCCAAATATGGTTCAATCGAAGTTCCTGTTTTCATGAAAGTTGAGAAATATCGCTACGTCATGCATATTACAAGTGA
AGTGACCGGCGAGCTTCGTCCTGAATTTACAGCTATGGATGCCTTACGCGCCACATTGCCAGCGGGGACGCTATCAGGAG
CACCGAAGCATCGAGCCTACCAAAGAATTTATGAATTTGAAACGCAAAAACGAGGAATTTATGGTGGAGCTATCGGTTAT
CTAACGAAAAATGGAAATTGCGATTTTGCAATTGCCATTCGGACAATGGTCTTGAAAGACAAGAAAGCACATGTTCAAGC
AGGAGCCGGAATTGTTTACGATTCAGTACCCGAACATGAATATCAAGAAACCTTAAACAAAGCCCAAGGCTTATTGAAAG
TTGGTCAATAA

Upstream 100 bases:

>100_bases
GAAAGGATTTCTAAAATGAACCAACGATGGCGAAGCTTAAATTTTAACTAATCAGTATCAATGGAAAAGACTACCTCAAA
CCTATAGATAGAGAGAAAAC

Downstream 100 bases:

>100_bases
ATAGACCCAAAAAGATGTAGAGATAAAAAATTTAACATCATCAGATAATTTTGTCAGTAATTTTTTACTGACAGAAAATA
AAAAATTACTGACAGGCCTT

Product: anthranilate synthase component I

Products: NA

Alternate protein names: Anthranilate synthase component I

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MRKIKEISADTMTPISVYLRLKGKNKVILESIPRENDQSRFSIIALNPVKHIKFTDGILSVNDEIISDENPMEFLEKLVC
QPESTDENLDLPFTSGAIGYAGFDTYGIFEGIQPELKDSIGTPDMYFMLYESALIFDHKREKLIFIEDNTYSQRSEKELQ
NALSANIESLSLLTEAENELTELSKLNFVSNMSQELFEEKVAKAKELIRNGDMFQVVLSQRLTADFTDNPFNYYRKLRVE
NPSSYMYFMEFDNFHVIGSSPERLVAVHGNQVSTNPIAGTRKRGQTEFEDQALIEDLESDPKEVAEHKMLVDLGRNDIGK
ISKYGSIEVPVFMKVEKYRYVMHITSEVTGELRPEFTAMDALRATLPAGTLSGAPKHRAYQRIYEFETQKRGIYGGAIGY
LTKNGNCDFAIAIRTMVLKDKKAHVQAGAGIVYDSVPEHEYQETLNKAQGLLKVGQ

Sequences:

>Translated_456_residues
MRKIKEISADTMTPISVYLRLKGKNKVILESIPRENDQSRFSIIALNPVKHIKFTDGILSVNDEIISDENPMEFLEKLVC
QPESTDENLDLPFTSGAIGYAGFDTYGIFEGIQPELKDSIGTPDMYFMLYESALIFDHKREKLIFIEDNTYSQRSEKELQ
NALSANIESLSLLTEAENELTELSKLNFVSNMSQELFEEKVAKAKELIRNGDMFQVVLSQRLTADFTDNPFNYYRKLRVE
NPSSYMYFMEFDNFHVIGSSPERLVAVHGNQVSTNPIAGTRKRGQTEFEDQALIEDLESDPKEVAEHKMLVDLGRNDIGK
ISKYGSIEVPVFMKVEKYRYVMHITSEVTGELRPEFTAMDALRATLPAGTLSGAPKHRAYQRIYEFETQKRGIYGGAIGY
LTKNGNCDFAIAIRTMVLKDKKAHVQAGAGIVYDSVPEHEYQETLNKAQGLLKVGQ
>Mature_456_residues
MRKIKEISADTMTPISVYLRLKGKNKVILESIPRENDQSRFSIIALNPVKHIKFTDGILSVNDEIISDENPMEFLEKLVC
QPESTDENLDLPFTSGAIGYAGFDTYGIFEGIQPELKDSIGTPDMYFMLYESALIFDHKREKLIFIEDNTYSQRSEKELQ
NALSANIESLSLLTEAENELTELSKLNFVSNMSQELFEEKVAKAKELIRNGDMFQVVLSQRLTADFTDNPFNYYRKLRVE
NPSSYMYFMEFDNFHVIGSSPERLVAVHGNQVSTNPIAGTRKRGQTEFEDQALIEDLESDPKEVAEHKMLVDLGRNDIGK
ISKYGSIEVPVFMKVEKYRYVMHITSEVTGELRPEFTAMDALRATLPAGTLSGAPKHRAYQRIYEFETQKRGIYGGAIGY
LTKNGNCDFAIAIRTMVLKDKKAHVQAGAGIVYDSVPEHEYQETLNKAQGLLKVGQ

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family

Homologues:

Organism=Escherichia coli, GI1787518, Length=373, Percent_Identity=39.9463806970509, Blast_Score=241, Evalue=7e-65,
Organism=Escherichia coli, GI1788114, Length=432, Percent_Identity=33.3333333333333, Blast_Score=234, Evalue=7e-63,
Organism=Escherichia coli, GI1786809, Length=365, Percent_Identity=26.027397260274, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI87082077, Length=260, Percent_Identity=28.8461538461538, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6320935, Length=455, Percent_Identity=39.7802197802198, Blast_Score=285, Evalue=1e-77,
Organism=Saccharomyces cerevisiae, GI6324361, Length=404, Percent_Identity=25, Blast_Score=124, Evalue=4e-29,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPE_LACLA (Q02001)

Other databases:

- EMBL:   M87483
- EMBL:   AE005176
- PIR:   F86808
- PIR:   S35124
- RefSeq:   NP_267626.1
- ProteinModelPortal:   Q02001
- SMR:   Q02001
- GeneID:   1115127
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0054
- NMPDR:   fig|272623.1.peg.1511
- HOGENOM:   HBG507440
- OMA:   SIPREND
- ProtClustDB:   PRK13570
- BioCyc:   LLAC272623:L0054-MONOMER
- BRENDA:   4.1.3.27
- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005256
- InterPro:   IPR015890
- Gene3D:   G3DSA:3.60.120.10
- PANTHER:   PTHR11236
- PRINTS:   PR00095
- TIGRFAMs:   TIGR00564

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind; SSF56322 TRPE_1_chor_bd

EC number: =4.1.3.27

Molecular weight: Translated: 51676; Mature: 51676

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKIKEISADTMTPISVYLRLKGKNKVILESIPRENDQSRFSIIALNPVKHIKFTDGILS
CCCHHHCCCCCCCEEEEEEEECCCCEEEEECCCCCCCCCEEEEEEECCCCEEEEECCEEE
VNDEIISDENPMEFLEKLVCQPESTDENLDLPFTSGAIGYAGFDTYGIFEGIQPELKDSI
CCCHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHCC
GTPDMYFMLYESALIFDHKREKLIFIEDNTYSQRSEKELQNALSANIESLSLLTEAENEL
CCCHHHHHHHHHHHEEECCCCEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHH
TELSKLNFVSNMSQELFEEKVAKAKELIRNGDMFQVVLSQRLTADFTDNPFNYYRKLRVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHEEEEC
NPSSYMYFMEFDNFHVIGSSPERLVAVHGNQVSTNPIAGTRKRGQTEFEDQALIEDLESD
CCCCEEEEEEECCEEEECCCCCEEEEEECCEECCCCCCCCCCCCCCCHHHHHHHHHHCCC
PKEVAEHKMLVDLGRNDIGKISKYGSIEVPVFMKVEKYRYVMHITSEVTGELRPEFTAMD
HHHHHHHHHHHHCCCCCHHHHHCCCCCCCEEEEEEEHEEEEEEEHHHHCCCCCCCHHHHH
ALRATLPAGTLSGAPKHRAYQRIYEFETQKRGIYGGAIGYLTKNGNCDFAIAIRTMVLKD
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEEHHHHCC
KKAHVQAGAGIVYDSVPEHEYQETLNKAQGLLKVGQ
CHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRKIKEISADTMTPISVYLRLKGKNKVILESIPRENDQSRFSIIALNPVKHIKFTDGILS
CCCHHHCCCCCCCEEEEEEEECCCCEEEEECCCCCCCCCEEEEEEECCCCEEEEECCEEE
VNDEIISDENPMEFLEKLVCQPESTDENLDLPFTSGAIGYAGFDTYGIFEGIQPELKDSI
CCCHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHCC
GTPDMYFMLYESALIFDHKREKLIFIEDNTYSQRSEKELQNALSANIESLSLLTEAENEL
CCCHHHHHHHHHHHEEECCCCEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHH
TELSKLNFVSNMSQELFEEKVAKAKELIRNGDMFQVVLSQRLTADFTDNPFNYYRKLRVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHEEEEC
NPSSYMYFMEFDNFHVIGSSPERLVAVHGNQVSTNPIAGTRKRGQTEFEDQALIEDLESD
CCCCEEEEEEECCEEEECCCCCEEEEEECCEECCCCCCCCCCCCCCCHHHHHHHHHHCCC
PKEVAEHKMLVDLGRNDIGKISKYGSIEVPVFMKVEKYRYVMHITSEVTGELRPEFTAMD
HHHHHHHHHHHHCCCCCHHHHHCCCCCCCEEEEEEEHEEEEEEEHHHHCCCCCCCHHHHH
ALRATLPAGTLSGAPKHRAYQRIYEFETQKRGIYGGAIGYLTKNGNCDFAIAIRTMVLKD
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEEHHHHCC
KKAHVQAGAGIVYDSVPEHEYQETLNKAQGLLKVGQ
CHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1400208; 11337471