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The map label for this gene is trpF

Identifier: 15673448

GI number: 15673448

Start: 1496508

End: 1497563

Strand: Reverse

Name: trpF

Synonym: L0050

Alternate gene names: 15673448

Gene position: 1497563-1496508 (Counterclockwise)

Preceding gene: 15673449

Following gene: 15673447

Centisome position: 63.31

GC content: 33.9

Gene sequence:

>1056_bases
ATGAAAATTAAAATCTGTGGCTTATCTACAAAAGAAGCTGTTGATACAGCTGTAGAATCTGGTGTCACACATCTCGGTTT
TATTCTTAGTCCCTCAAAACGCCAAGTTGCACCAGAAAAAATTCTTCAAATCACAAACGATGTCCCAAAAACAGTCAAAA
AAGTAGGAGTTTTTGTTGATGAACCTATTGATTTTGTAAAAAAAGCCATTCAAGTTGCTCAACTCGATCTGGTTCAGCTT
CACGGAAATGAAGATATGAATTACATTAATCAACTAGATATTTCGGTTATTAAAGCAATAAGACCAGACCAAGAATTTAA
AGAATACGAAGATGTAATTTTATTATTTGATAGTCCACAAGCTGGAAGTGGTCAAGCATTTGATTGGGACTCTTTGGTGA
CCAGCGGTCTGAAAAATAAATTTTTCATCGCTGGTGGACTTAATCCAGAAAATGTAGCAGCTGCTATTCAACATTTTCCA
AATGCCTACGGTGTGGATGTTTCTTCTGGAGTAGAAACTGACGGAATTAAAAACCTTACAAAAATAAAAAACTTTGTTCA
AAATGCAAGCCTTGCCTCATCAAAGCAATTATTTATAGAATTTTTAAGAATCACAAAAAAGCTAAATGAAAATAAGATTA
TCCCTTATTTAATGGGAAGTTTAGCAGTTGAGCAAATAATCAATTTTCCAACAAATCCTGATGACATTGATATTCAACTC
AAAACGTCTGATTTTGAAAATTTTGAGCAATTAACAAGTTTAATGGAAAAATTAGGTTATCAGCTTATTGACTTACATGA
GCATAAATTTGAAAAAGCTAGTATTCATGTTGGCTTTGCAAGTGTGGAGACCCTTAAAAACTATGCCGGGGTTGACTATT
TGACCATTCAACAAGAAAGAATGGAAAATGGCGAAAAATATCATCTTCCAAATGTTGAACAATCCCTTAAAATCTATGAG
GCAGCAAAACGAGATGAGTGGCGAGGAGGGAAGCAAAAAGATTCCTTTATTTTCGATGAGTTAATAAAGGAACAGAAGAG
GAATGACAATGAATGA

Upstream 100 bases:

>100_bases
GTTTCCGATACTTTCAATGGAATATTGGTTGGAACAGCACTCATGCAATCAGAAAATCTAGAAAAATCTTTGAAAGATTT
AAAAGTCAAGAGGAAAACGA

Downstream 100 bases:

>100_bases
TAATCTTATTGAAGAGGGTGTAGAGATTCGAAATGGTCTCATTATTAAGTCAATTCAAAAAGAAGATATATTAGAGCTTT
GGCAAATTAGTTATGGACCT

Product: phosphorybosyl-anthranilate isomerase

Products: NA

Alternate protein names: PRAI

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MKIKICGLSTKEAVDTAVESGVTHLGFILSPSKRQVAPEKILQITNDVPKTVKKVGVFVDEPIDFVKKAIQVAQLDLVQL
HGNEDMNYINQLDISVIKAIRPDQEFKEYEDVILLFDSPQAGSGQAFDWDSLVTSGLKNKFFIAGGLNPENVAAAIQHFP
NAYGVDVSSGVETDGIKNLTKIKNFVQNASLASSKQLFIEFLRITKKLNENKIIPYLMGSLAVEQIINFPTNPDDIDIQL
KTSDFENFEQLTSLMEKLGYQLIDLHEHKFEKASIHVGFASVETLKNYAGVDYLTIQQERMENGEKYHLPNVEQSLKIYE
AAKRDEWRGGKQKDSFIFDELIKEQKRNDNE

Sequences:

>Translated_351_residues
MKIKICGLSTKEAVDTAVESGVTHLGFILSPSKRQVAPEKILQITNDVPKTVKKVGVFVDEPIDFVKKAIQVAQLDLVQL
HGNEDMNYINQLDISVIKAIRPDQEFKEYEDVILLFDSPQAGSGQAFDWDSLVTSGLKNKFFIAGGLNPENVAAAIQHFP
NAYGVDVSSGVETDGIKNLTKIKNFVQNASLASSKQLFIEFLRITKKLNENKIIPYLMGSLAVEQIINFPTNPDDIDIQL
KTSDFENFEQLTSLMEKLGYQLIDLHEHKFEKASIHVGFASVETLKNYAGVDYLTIQQERMENGEKYHLPNVEQSLKIYE
AAKRDEWRGGKQKDSFIFDELIKEQKRNDNE
>Mature_351_residues
MKIKICGLSTKEAVDTAVESGVTHLGFILSPSKRQVAPEKILQITNDVPKTVKKVGVFVDEPIDFVKKAIQVAQLDLVQL
HGNEDMNYINQLDISVIKAIRPDQEFKEYEDVILLFDSPQAGSGQAFDWDSLVTSGLKNKFFIAGGLNPENVAAAIQHFP
NAYGVDVSSGVETDGIKNLTKIKNFVQNASLASSKQLFIEFLRITKKLNENKIIPYLMGSLAVEQIINFPTNPDDIDIQL
KTSDFENFEQLTSLMEKLGYQLIDLHEHKFEKASIHVGFASVETLKNYAGVDYLTIQQERMENGEKYHLPNVEQSLKIYE
AAKRDEWRGGKQKDSFIFDELIKEQKRNDNE

Specific function: Bifunctional Enzyme That Catalyzes Two Sequential Steps Of Tryptophan Biosynthetic Pathway. The First Reaction Is Catalyzed By The Isomerase, Coded By The Trpf Domain; The Second Reaction Is Catalyzed By The Synthase, Coded By The Trpc Domain. [C]

COG id: COG0135

COG function: function code E; Phosphoribosylanthranilate isomerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To B.subtilis ynaI in the C-terminal section

Homologues:

Organism=Escherichia coli, GI87081863, Length=199, Percent_Identity=34.1708542713568, Blast_Score=84, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6320210, Length=210, Percent_Identity=34.2857142857143, Blast_Score=103, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRPF_LACLA (Q02002)

Other databases:

- EMBL:   M87483
- EMBL:   AE005176
- PIR:   S35128
- RefSeq:   NP_267622.1
- ProteinModelPortal:   Q02002
- SMR:   Q02002
- GeneID:   1115123
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0050
- NMPDR:   fig|272623.1.peg.1507
- HOGENOM:   HBG108006
- OMA:   HEHKFEK
- ProtClustDB:   CLSK697758
- BioCyc:   LLAC272623:L0050-MONOMER
- BRENDA:   5.3.1.24
- HAMAP:   MF_00135_B
- InterPro:   IPR013785
- InterPro:   IPR001240
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF00697 PRAI; SSF51366 RibP_bind_barrel

EC number: =5.3.1.24

Molecular weight: Translated: 39586; Mature: 39586

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKICGLSTKEAVDTAVESGVTHLGFILSPSKRQVAPEKILQITNDVPKTVKKVGVFVD
CEEEEECCCHHHHHHHHHHHCCHHHHEEECCCHHCCCHHHHHHHHHHHHHHHHHHCCHHC
EPIDFVKKAIQVAQLDLVQLHGNEDMNYINQLDISVIKAIRPDQEFKEYEDVILLFDSPQ
CCHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCEEEEECCCC
AGSGQAFDWDSLVTSGLKNKFFIAGGLNPENVAAAIQHFPNAYGVDVSSGVETDGIKNLT
CCCCCCCCHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
KIKNFVQNASLASSKQLFIEFLRITKKLNENKIIPYLMGSLAVEQIINFPTNPDDIDIQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEE
KTSDFENFEQLTSLMEKLGYQLIDLHEHKFEKASIHVGFASVETLKNYAGVDYLTIQQER
ECCCHHHHHHHHHHHHHHCCHHHHHHHHHCCHHEEEEEHHHHHHHHHHCCCCEEEEEHHH
MENGEKYHLPNVEQSLKIYEAAKRDEWRGGKQKDSFIFDELIKEQKRNDNE
HHCCCEECCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKIKICGLSTKEAVDTAVESGVTHLGFILSPSKRQVAPEKILQITNDVPKTVKKVGVFVD
CEEEEECCCHHHHHHHHHHHCCHHHHEEECCCHHCCCHHHHHHHHHHHHHHHHHHCCHHC
EPIDFVKKAIQVAQLDLVQLHGNEDMNYINQLDISVIKAIRPDQEFKEYEDVILLFDSPQ
CCHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCEEEEECCCC
AGSGQAFDWDSLVTSGLKNKFFIAGGLNPENVAAAIQHFPNAYGVDVSSGVETDGIKNLT
CCCCCCCCHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
KIKNFVQNASLASSKQLFIEFLRITKKLNENKIIPYLMGSLAVEQIINFPTNPDDIDIQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEE
KTSDFENFEQLTSLMEKLGYQLIDLHEHKFEKASIHVGFASVETLKNYAGVDYLTIQQER
ECCCHHHHHHHHHHHHHHCCHHHHHHHHHCCHHEEEEEHHHHHHHHHHCCCCEEEEEHHH
MENGEKYHLPNVEQSLKIYEAAKRDEWRGGKQKDSFIFDELIKEQKRNDNE
HHCCCEECCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1400208; 11337471