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The map label for this gene is sbcD

Identifier: 15673304

GI number: 15673304

Start: 1355986

End: 1357158

Strand: Reverse

Name: sbcD

Synonym: L0327

Alternate gene names: 15673304

Gene position: 1357158-1355986 (Counterclockwise)

Preceding gene: 15673305

Following gene: 15673303

Centisome position: 57.37

GC content: 32.82

Gene sequence:

>1173_bases
ATGAAATTTTTACACACATCAGACTGGCATATTGGAAGAACACTCAATGGTTTTTCTTTATTGGAAGAACAAAAATATGC
ATTTGAGCAAATTTTATCTTTGGCAAAACAAAATCAAGTTGATGGAATTATAATTGCTGGAGATCTTTATGAACGAGCAG
TTCCGAGTGCAGAATCAGTCATAACATTCAATCAGATGCTGAGAGAGATGAATATTATAGAAAAGTTTCCTATTTATATG
ATTTCAGGGAATCATGATGGAGCCAAAAGACTAAGTTATGCTAAGGACTGGTTGGATTATAATAATTTACATCTACGAAC
ATCCCTTGAGGAAGCCTTTATTCCAGTGGAAACAAAAGAAACTCAGATTTTTTTACTTCCCTTTTTTGACCCAATGGATG
CAAGAATATATTTTTCGAATCAGGGATTAGATGAAGAGGACACAAAGCAAATTAAGACAATTGATGATGCGATGACATTG
GTAATTTCTAAGATGAAAAGTCAGTTCAATAATAAGAAAAATCAGGTTTTAGTCACTCATTTTGCGGTGAGTCCTCATAA
AGAAGAAATTGTTTTAACAAGTGAAACCCCATCTAAAGTAGGTGGATTATCGACCTTAAATGTTTCACAATTTGCAGATT
TTGATTATGTTGCGTTAGGTCATATTCATACGAGGTTAGCTTCCCCAAGTGATAAAGTACAGTATTCGGGGAGTCCAGTA
AAATTTAATACCAAAGAAGCGTCAACACAAAAAGGAATCTTTGTTGTTGAAATTAAAGAAAAAATGCTCAATTCTAACTT
TCTTCCACTACAAGTTAAAACAGATTTAGTGGTTCTTGAAGAAGAATGGGAGCAACTCATAAGCCGTGATTTTTATGAAA
GGCAACCACTTAATAAAGCTTGGTTTGCGATTACAATTAAAAATTTTGATCGGACGAAACTCGTGGGACAAAATTTACGT
GCCCAACTCCAAAAGATTTATGGAACAATTATAGAACTTGACTATCAATTTGAAAAAAAACAATTAACAAGAAGTAGGAG
TCAAGATATTGAAACGTTAGATAATGTGGAAATTCTTTCTAGTTTTTATAAATTTACTACAGGGGAAACGCTAGATGATG
TACAAACAGCAATCATCGAAAGTACATTTAATGAATTAGAAGGAGAAAAATAA

Upstream 100 bases:

>100_bases
TATCAGATACTCTATCAATGAATAATATAAAACTATTTGATAATGTTATAATGAAAGGTGAATATTATAAGGATTAAGAT
TATTTTTGGGGGATAGAGAT

Downstream 100 bases:

>100_bases
AAATGAAGCCTATATATTTGGAAATGAATTATTTTGGCCCCCATGAGAATTCTGTCGTAGATTTTCGACTGCTAGATGAA
TCTCCTATATTTCTAATTAG

Product: exonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MKFLHTSDWHIGRTLNGFSLLEEQKYAFEQILSLAKQNQVDGIIIAGDLYERAVPSAESVITFNQMLREMNIIEKFPIYM
ISGNHDGAKRLSYAKDWLDYNNLHLRTSLEEAFIPVETKETQIFLLPFFDPMDARIYFSNQGLDEEDTKQIKTIDDAMTL
VISKMKSQFNNKKNQVLVTHFAVSPHKEEIVLTSETPSKVGGLSTLNVSQFADFDYVALGHIHTRLASPSDKVQYSGSPV
KFNTKEASTQKGIFVVEIKEKMLNSNFLPLQVKTDLVVLEEEWEQLISRDFYERQPLNKAWFAITIKNFDRTKLVGQNLR
AQLQKIYGTIIELDYQFEKKQLTRSRSQDIETLDNVEILSSFYKFTTGETLDDVQTAIIESTFNELEGEK

Sequences:

>Translated_390_residues
MKFLHTSDWHIGRTLNGFSLLEEQKYAFEQILSLAKQNQVDGIIIAGDLYERAVPSAESVITFNQMLREMNIIEKFPIYM
ISGNHDGAKRLSYAKDWLDYNNLHLRTSLEEAFIPVETKETQIFLLPFFDPMDARIYFSNQGLDEEDTKQIKTIDDAMTL
VISKMKSQFNNKKNQVLVTHFAVSPHKEEIVLTSETPSKVGGLSTLNVSQFADFDYVALGHIHTRLASPSDKVQYSGSPV
KFNTKEASTQKGIFVVEIKEKMLNSNFLPLQVKTDLVVLEEEWEQLISRDFYERQPLNKAWFAITIKNFDRTKLVGQNLR
AQLQKIYGTIIELDYQFEKKQLTRSRSQDIETLDNVEILSSFYKFTTGETLDDVQTAIIESTFNELEGEK
>Mature_390_residues
MKFLHTSDWHIGRTLNGFSLLEEQKYAFEQILSLAKQNQVDGIIIAGDLYERAVPSAESVITFNQMLREMNIIEKFPIYM
ISGNHDGAKRLSYAKDWLDYNNLHLRTSLEEAFIPVETKETQIFLLPFFDPMDARIYFSNQGLDEEDTKQIKTIDDAMTL
VISKMKSQFNNKKNQVLVTHFAVSPHKEEIVLTSETPSKVGGLSTLNVSQFADFDYVALGHIHTRLASPSDKVQYSGSPV
KFNTKEASTQKGIFVVEIKEKMLNSNFLPLQVKTDLVVLEEEWEQLISRDFYERQPLNKAWFAITIKNFDRTKLVGQNLR
AQLQKIYGTIIELDYQFEKKQLTRSRSQDIETLDNVEILSSFYKFTTGETLDDVQTAIIESTFNELEGEK

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=410, Percent_Identity=24.6341463414634, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 44902; Mature: 44902

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFLHTSDWHIGRTLNGFSLLEEQKYAFEQILSLAKQNQVDGIIIAGDLYERAVPSAESV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHCCCCHHHH
ITFNQMLREMNIIEKFPIYMISGNHDGAKRLSYAKDWLDYNNLHLRTSLEEAFIPVETKE
HHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEECHHHHCCCEECCC
TQIFLLPFFDPMDARIYFSNQGLDEEDTKQIKTIDDAMTLVISKMKSQFNNKKNQVLVTH
CEEEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
FAVSPHKEEIVLTSETPSKVGGLSTLNVSQFADFDYVALGHIHTRLASPSDKVQYSGSPV
EEECCCCCEEEEECCCCHHHCCCEEECHHHHCCCCEEEHHHHHHHHCCCCCCEEECCCCE
KFNTKEASTQKGIFVVEIKEKMLNSNFLPLQVKTDLVVLEEEWEQLISRDFYERQPLNKA
EECCCCCCCCCCEEEEEEHHHHHCCCCCEEEEECCEEEEHHHHHHHHHHHHHHCCCCCCE
WFAITIKNFDRTKLVGQNLRAQLQKIYGTIIELDYQFEKKQLTRSRSQDIETLDNVEILS
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHHHHHHHHHHHH
SFYKFTTGETLDDVQTAIIESTFNELEGEK
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKFLHTSDWHIGRTLNGFSLLEEQKYAFEQILSLAKQNQVDGIIIAGDLYERAVPSAESV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHCCCCHHHH
ITFNQMLREMNIIEKFPIYMISGNHDGAKRLSYAKDWLDYNNLHLRTSLEEAFIPVETKE
HHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEECHHHHCCCEECCC
TQIFLLPFFDPMDARIYFSNQGLDEEDTKQIKTIDDAMTLVISKMKSQFNNKKNQVLVTH
CEEEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
FAVSPHKEEIVLTSETPSKVGGLSTLNVSQFADFDYVALGHIHTRLASPSDKVQYSGSPV
EEECCCCCEEEEECCCCHHHCCCEEECHHHHCCCCEEEHHHHHHHHCCCCCCEEECCCCE
KFNTKEASTQKGIFVVEIKEKMLNSNFLPLQVKTDLVVLEEEWEQLISRDFYERQPLNKA
EECCCCCCCCCCEEEEEEHHHHHCCCCCEEEEECCEEEEHHHHHHHHHHHHHHCCCCCCE
WFAITIKNFDRTKLVGQNLRAQLQKIYGTIIELDYQFEKKQLTRSRSQDIETLDNVEILS
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHHHHHHHHHHHH
SFYKFTTGETLDDVQTAIIESTFNELEGEK
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA