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The map label for this gene is panE

Identifier: 15673305

GI number: 15673305

Start: 1357310

End: 1358248

Strand: Reverse

Name: panE

Synonym: L157055

Alternate gene names: 15673305

Gene position: 1358248-1357310 (Counterclockwise)

Preceding gene: 15673306

Following gene: 15673304

Centisome position: 57.42

GC content: 33.55

Gene sequence:

>939_bases
ATGAGAATTACAATTGCCGGTGCAGGAGCGATGGGGAGTCGTTTTGGTTTAATGCTTCATAAAGGTGGCAATGAAGTAAC
CCTTATAGATGGATGGCCTGAACACGTTAAAGCGATTAAAGAGCATGGTTTGCGAGCTAATTACAATGGAGAAGAACTCA
CCGCTCATCTATCGGTTGAGTTACAATCTGAGATTTCTTCTAAAGAAAAAACAGATTTAATTATTTTGTTTACAAAAGCC
ATGCAATTAGATAAGATGCTACAAGATATTAAACCATTAATTGACGAGCATACCAAGGTACTTTGCTTACTAAATGGAAT
TGGTCACGAAGATACTATAGAAAAATATGTTTCGAAAAATAATATCTTTATTGGTAATACTATGTGGACTGCTGGATTAG
AAGGTCCAGGTAAAGCTAAATTATTTGGTGATGGTTCGGTTGAGCTACAAAATCTTATTTCAGGTGAGGAAGAAACAGCT
AAAAAGTTAGCAGAAATATTATCAGAATCGGGACTGAATGCTAAATATTCTAACAATATTCATTATTCTATTTATAGAAA
AGCTTGTGTTAATGGAACAATGAATGGGCTTTGTACTATTTTAGACACTAATATGGCCGGATTAGGTGAAACAAAACCAG
CACATGATATGGTTGTTACTATTGTTAATGAATTTGCAGCAGTAGCAAAATTTGAGAATGTAAACCTTGATATTGCTGAA
GTAGTTCAGCACGTTGAAACATGTTTTGATCCATCTACAATTGGATTACATTACCCTTCTATGTATCAGGATTTGATTAA
AAATAATCGATTGACAGAGATTGACTATATTAATGGGGCTGTTTCACGTAAAGGTAAAAAATATAATGTAGCCACACCTT
ATTGTGATTTCTTAACACAATTAGTTCACAGCAAAGAAGAGTTATTAAAAGCAAAATAA

Upstream 100 bases:

>100_bases
CTATAAATAATTTGTTATCTATAAATTACTATAACTGAACTCGTTTAAAAATAGCGAATTCAGTTATTTTATGGAAAAAA
TATGTAGATTGAGAGATAAA

Downstream 100 bases:

>100_bases
TAAATAATTGAATTTATTGAAATACTAGTGTTAATGCTAGTATTTTTGTTTTATCAGATACTCTATCAATGAATAATATA
AAACTATTTGATAATGTTAT

Product: 2-dehydropantoate 2-reductase

Products: NA

Alternate protein names: Ketopantoate reductase; KPA reductase; KPR

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MRITIAGAGAMGSRFGLMLHKGGNEVTLIDGWPEHVKAIKEHGLRANYNGEELTAHLSVELQSEISSKEKTDLIILFTKA
MQLDKMLQDIKPLIDEHTKVLCLLNGIGHEDTIEKYVSKNNIFIGNTMWTAGLEGPGKAKLFGDGSVELQNLISGEEETA
KKLAEILSESGLNAKYSNNIHYSIYRKACVNGTMNGLCTILDTNMAGLGETKPAHDMVVTIVNEFAAVAKFENVNLDIAE
VVQHVETCFDPSTIGLHYPSMYQDLIKNNRLTEIDYINGAVSRKGKKYNVATPYCDFLTQLVHSKEELLKAK

Sequences:

>Translated_312_residues
MRITIAGAGAMGSRFGLMLHKGGNEVTLIDGWPEHVKAIKEHGLRANYNGEELTAHLSVELQSEISSKEKTDLIILFTKA
MQLDKMLQDIKPLIDEHTKVLCLLNGIGHEDTIEKYVSKNNIFIGNTMWTAGLEGPGKAKLFGDGSVELQNLISGEEETA
KKLAEILSESGLNAKYSNNIHYSIYRKACVNGTMNGLCTILDTNMAGLGETKPAHDMVVTIVNEFAAVAKFENVNLDIAE
VVQHVETCFDPSTIGLHYPSMYQDLIKNNRLTEIDYINGAVSRKGKKYNVATPYCDFLTQLVHSKEELLKAK
>Mature_312_residues
MRITIAGAGAMGSRFGLMLHKGGNEVTLIDGWPEHVKAIKEHGLRANYNGEELTAHLSVELQSEISSKEKTDLIILFTKA
MQLDKMLQDIKPLIDEHTKVLCLLNGIGHEDTIEKYVSKNNIFIGNTMWTAGLEGPGKAKLFGDGSVELQNLISGEEETA
KKLAEILSESGLNAKYSNNIHYSIYRKACVNGTMNGLCTILDTNMAGLGETKPAHDMVVTIVNEFAAVAKFENVNLDIAE
VVQHVETCFDPSTIGLHYPSMYQDLIKNNRLTEIDYINGAVSRKGKKYNVATPYCDFLTQLVHSKEELLKAK

Specific function: Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid

COG id: COG1893

COG function: function code H; Ketopantoate reductase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ketopantoate reductase family

Homologues:

Organism=Escherichia coli, GI1786627, Length=316, Percent_Identity=26.5822784810127, Blast_Score=78, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PANE_LACLA (Q9CFY8)

Other databases:

- EMBL:   AE005176
- PIR:   C86790
- RefSeq:   NP_267479.1
- ProteinModelPortal:   Q9CFY8
- SMR:   Q9CFY8
- GeneID:   1114972
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L157055
- NMPDR:   fig|272623.1.peg.1358
- HOGENOM:   HBG668370
- OMA:   VWATIRE
- ProtClustDB:   PRK06522
- BioCyc:   LLAC272623:L157055-MONOMER
- BRENDA:   1.1.1.169
- GO:   GO:0005737
- InterPro:   IPR008927
- InterPro:   IPR003710
- InterPro:   IPR013752
- InterPro:   IPR013332
- InterPro:   IPR013328
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- Gene3D:   G3DSA:1.10.1040.10
- PANTHER:   PTHR21708:SF21
- TIGRFAMs:   TIGR00745

Pfam domain/function: PF02558 ApbA; PF08546 ApbA_C; SSF48179 6DGDH_C_like

EC number: =1.1.1.169

Molecular weight: Translated: 34444; Mature: 34444

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: ACT_SITE 187-187 BINDING 105-105 BINDING 105-105 BINDING 191-191 BINDING 195-195 BINDING 260-260 BINDING 273-273

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRITIAGAGAMGSRFGLMLHKGGNEVTLIDGWPEHVKAIKEHGLRANYNGEELTAHLSVE
CEEEEEECCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHCCCCCCCCCHHEEEEEEEE
LQSEISSKEKTDLIILFTKAMQLDKMLQDIKPLIDEHTKVLCLLNGIGHEDTIEKYVSKN
EHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHCC
NIFIGNTMWTAGLEGPGKAKLFGDGSVELQNLISGEEETAKKLAEILSESGLNAKYSNNI
CEEEECCEEECCCCCCCCEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCEECCCE
HYSIYRKACVNGTMNGLCTILDTNMAGLGETKPAHDMVVTIVNEFAAVAKFENVNLDIAE
EEEEHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHH
VVQHVETCFDPSTIGLHYPSMYQDLIKNNRLTEIDYINGAVSRKGKKYNVATPYCDFLTQ
HHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEHHCCCHHHCCCCEECCCCHHHHHHHH
LVHSKEELLKAK
HHHCHHHHHCCC
>Mature Secondary Structure
MRITIAGAGAMGSRFGLMLHKGGNEVTLIDGWPEHVKAIKEHGLRANYNGEELTAHLSVE
CEEEEEECCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHCCCCCCCCCHHEEEEEEEE
LQSEISSKEKTDLIILFTKAMQLDKMLQDIKPLIDEHTKVLCLLNGIGHEDTIEKYVSKN
EHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHCC
NIFIGNTMWTAGLEGPGKAKLFGDGSVELQNLISGEEETAKKLAEILSESGLNAKYSNNI
CEEEECCEEECCCCCCCCEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCEECCCE
HYSIYRKACVNGTMNGLCTILDTNMAGLGETKPAHDMVVTIVNEFAAVAKFENVNLDIAE
EEEEHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHH
VVQHVETCFDPSTIGLHYPSMYQDLIKNNRLTEIDYINGAVSRKGKKYNVATPYCDFLTQ
HHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEHHCCCHHHCCCCEECCCCHHHHHHHH
LVHSKEELLKAK
HHHCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471