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The map label for this gene is glyA

Identifier: 15672583

GI number: 15672583

Start: 590943

End: 592190

Strand: Direct

Name: glyA

Synonym: L0082

Alternate gene names: 15672583

Gene position: 590943-592190 (Clockwise)

Preceding gene: 15672582

Following gene: 15672584

Centisome position: 24.98

GC content: 37.98

Gene sequence:

>1248_bases
ATGATTTTTGATAAAGAAGATTTTGAAAGTTTTGACCCAGAATTGTGGGCAGCCATTCATGCTGAGGAAATTCGTCAGCA
ACAAAATATTGAATTAATTGCTTCAGAAAATATTGTTTCAAAAGCAGTTATGGCAGCACAAGGTTCTGTTTTGACAAATA
AATATGCCGAAGGTTATCCTGGGAAACGTTATTATGGCGGAACAGAAGCGGTTGATGTTGTAGAAAATTTGGCTATTGAA
CGAGCAAAAGAACTTTTCGGTGCAAAATTTGCCAATGTTCAACCTCATTCAGGTTCTCAAGCCAATGCGGCAGCTTATAT
GGCATTAATTCAACCTGGAGACACAGTCTTAGGAATGGATTTAAATGCTGGCGGTCACTTGACTCATGGGGCTTCAGTCA
ATTTTTCAGGAAAAACTTATCATTTTGTTCCTTATGGAGTTAATTCTGAAACTGAGCTTTTAGATTATGATGAAATTTTA
AAAATTGCTAAACAAGTTCAACCTAAACTGATTGTTGCTGGCGCTTCTGCCTATTCACGCCTGATTGATTTTGCTAAGTT
CCGTGAAATCGCTGATAGTGTTGGAGCAAAATTAATGGTTGATATGGCACATATTGCTGGATTGGTAGCGACTGGTGCGC
ATCCTAATCCACTTCCTTATGCTGATGTCGTTACAACAACAACTCATAAGACACTCCGTGGACCACGTGGTGGAATGATT
TTGACTAACGATGAAGCTTTGGCCAAGAAAATTAACTCTGCAATCTTCCCTGGAACTCAAGGTGGGCCTTTAGAACATGT
CATTGCAGCAAAAGCAGTAGCTTTTAAAGAAGCACTTGATCCAGAATTCACAACCTATATTGAACAAGTCATTAAAAATA
CGCAAGCAATGGCTGAGGAATTTGCTAAAGTGGAAGGCTTACGATTGATAGCTGGTGGTTCAGATAATCATTTGCTTAAT
CTTAAAGTTTTAGACTTAGGAATTAATGGGAAAGAAGCTCAAGATTTATTGGATAGTGTTCACATCACACTTAATAAAGA
AGCAATTCCTGATGAAACATTATCACCATTTAAAACTTCAGGGGTGCGAATTGGAGCAGCAGCAATTACATCAAGAGGAT
TTAAAGAAGTTGAAGCGAAAAAAGTTGCACAATTGGTCTCAGAAGCTTTGGTTAATCACGATAATCAAGAAAAACTTGCG
GAAGTTAGGAAAGCAGCTTTAGAACTGACTCGTCAATTTCCCTTATAA

Upstream 100 bases:

>100_bases
AAAGTAAACTAAAATTAACTGATTAAGTTTATAGCGCCAATGACTATAAAATTCTACTTATATTTTTTAAAATATCCCAA
AAGACATACAAGGAGAACCA

Downstream 100 bases:

>100_bases
TATCAAAAATTAGACAAAGAAAATTTGTCTAATTTTTTAATGTTAAGCTAAATTTAAAAGAGAAGTGTTATAATAGAGCT
TATATAAAAAATGACTGCAA

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MIFDKEDFESFDPELWAAIHAEEIRQQQNIELIASENIVSKAVMAAQGSVLTNKYAEGYPGKRYYGGTEAVDVVENLAIE
RAKELFGAKFANVQPHSGSQANAAAYMALIQPGDTVLGMDLNAGGHLTHGASVNFSGKTYHFVPYGVNSETELLDYDEIL
KIAKQVQPKLIVAGASAYSRLIDFAKFREIADSVGAKLMVDMAHIAGLVATGAHPNPLPYADVVTTTTHKTLRGPRGGMI
LTNDEALAKKINSAIFPGTQGGPLEHVIAAKAVAFKEALDPEFTTYIEQVIKNTQAMAEEFAKVEGLRLIAGGSDNHLLN
LKVLDLGINGKEAQDLLDSVHITLNKEAIPDETLSPFKTSGVRIGAAAITSRGFKEVEAKKVAQLVSEALVNHDNQEKLA
EVRKAALELTRQFPL

Sequences:

>Translated_415_residues
MIFDKEDFESFDPELWAAIHAEEIRQQQNIELIASENIVSKAVMAAQGSVLTNKYAEGYPGKRYYGGTEAVDVVENLAIE
RAKELFGAKFANVQPHSGSQANAAAYMALIQPGDTVLGMDLNAGGHLTHGASVNFSGKTYHFVPYGVNSETELLDYDEIL
KIAKQVQPKLIVAGASAYSRLIDFAKFREIADSVGAKLMVDMAHIAGLVATGAHPNPLPYADVVTTTTHKTLRGPRGGMI
LTNDEALAKKINSAIFPGTQGGPLEHVIAAKAVAFKEALDPEFTTYIEQVIKNTQAMAEEFAKVEGLRLIAGGSDNHLLN
LKVLDLGINGKEAQDLLDSVHITLNKEAIPDETLSPFKTSGVRIGAAAITSRGFKEVEAKKVAQLVSEALVNHDNQEKLA
EVRKAALELTRQFPL
>Mature_415_residues
MIFDKEDFESFDPELWAAIHAEEIRQQQNIELIASENIVSKAVMAAQGSVLTNKYAEGYPGKRYYGGTEAVDVVENLAIE
RAKELFGAKFANVQPHSGSQANAAAYMALIQPGDTVLGMDLNAGGHLTHGASVNFSGKTYHFVPYGVNSETELLDYDEIL
KIAKQVQPKLIVAGASAYSRLIDFAKFREIADSVGAKLMVDMAHIAGLVATGAHPNPLPYADVVTTTTHKTLRGPRGGMI
LTNDEALAKKINSAIFPGTQGGPLEHVIAAKAVAFKEALDPEFTTYIEQVIKNTQAMAEEFAKVEGLRLIAGGSDNHLLN
LKVLDLGINGKEAQDLLDSVHITLNKEAIPDETLSPFKTSGVRIGAAAITSRGFKEVEAKKVAQLVSEALVNHDNQEKLA
EVRKAALELTRQFPL

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI19923315, Length=455, Percent_Identity=42.4175824175824, Blast_Score=358, Evalue=7e-99,
Organism=Homo sapiens, GI261862352, Length=455, Percent_Identity=42.4175824175824, Blast_Score=358, Evalue=7e-99,
Organism=Homo sapiens, GI261862350, Length=455, Percent_Identity=42.4175824175824, Blast_Score=358, Evalue=7e-99,
Organism=Homo sapiens, GI261862348, Length=455, Percent_Identity=42.4175824175824, Blast_Score=358, Evalue=7e-99,
Organism=Homo sapiens, GI261862346, Length=455, Percent_Identity=41.5384615384615, Blast_Score=340, Evalue=1e-93,
Organism=Homo sapiens, GI22547186, Length=405, Percent_Identity=44.4444444444444, Blast_Score=315, Evalue=4e-86,
Organism=Homo sapiens, GI22547189, Length=388, Percent_Identity=41.7525773195876, Blast_Score=281, Evalue=5e-76,
Organism=Escherichia coli, GI1788902, Length=417, Percent_Identity=57.3141486810552, Blast_Score=472, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI25144732, Length=404, Percent_Identity=46.7821782178218, Blast_Score=355, Evalue=2e-98,
Organism=Caenorhabditis elegans, GI25144729, Length=404, Percent_Identity=46.7821782178218, Blast_Score=355, Evalue=3e-98,
Organism=Saccharomyces cerevisiae, GI6319739, Length=416, Percent_Identity=44.7115384615385, Blast_Score=353, Evalue=4e-98,
Organism=Saccharomyces cerevisiae, GI6323087, Length=402, Percent_Identity=45.273631840796, Blast_Score=332, Evalue=5e-92,
Organism=Drosophila melanogaster, GI24640005, Length=449, Percent_Identity=42.9844097995546, Blast_Score=353, Evalue=9e-98,
Organism=Drosophila melanogaster, GI221329721, Length=449, Percent_Identity=42.5389755011136, Blast_Score=353, Evalue=2e-97,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_LACLA (Q9CHW7)

Other databases:

- EMBL:   AE005176
- PIR:   A86700
- RefSeq:   NP_266757.1
- ProteinModelPortal:   Q9CHW7
- SMR:   Q9CHW7
- GeneID:   1114221
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0082
- NMPDR:   fig|272623.1.peg.616
- HOGENOM:   HBG301263
- OMA:   ADVEANV
- ProtClustDB:   PRK00011
- BioCyc:   LLAC272623:L0082-MONOMER
- BRENDA:   2.1.2.1
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 44790; Mature: 44790

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFDKEDFESFDPELWAAIHAEEIRQQQNIELIASENIVSKAVMAAQGSVLTNKYAEGYP
CCCCCCHHHHCCHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHCCCHHHHHHHCCCC
GKRYYGGTEAVDVVENLAIERAKELFGAKFANVQPHSGSQANAAAYMALIQPGDTVLGMD
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEE
LNAGGHLTHGASVNFSGKTYHFVPYGVNSETELLDYDEILKIAKQVQPKLIVAGASAYSR
ECCCCCEECCCEEEECCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEECCHHHHH
LIDFAKFREIADSVGAKLMVDMAHIAGLVATGAHPNPLPYADVVTTTTHKTLRGPRGGMI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEECCCHHHHCCCCCCEE
LTNDEALAKKINSAIFPGTQGGPLEHVIAAKAVAFKEALDPEFTTYIEQVIKNTQAMAEE
EECCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
FAKVEGLRLIAGGSDNHLLNLKVLDLGINGKEAQDLLDSVHITLNKEAIPDETLSPFKTS
HHHHCCEEEEECCCCCEEEEEEEEEECCCCHHHHHHHHHHEEEECCCCCCCCCCCCCHHC
GVRIGAAAITSRGFKEVEAKKVAQLVSEALVNHDNQEKLAEVRKAALELTRQFPL
CEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIFDKEDFESFDPELWAAIHAEEIRQQQNIELIASENIVSKAVMAAQGSVLTNKYAEGYP
CCCCCCHHHHCCHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHCCCHHHHHHHCCCC
GKRYYGGTEAVDVVENLAIERAKELFGAKFANVQPHSGSQANAAAYMALIQPGDTVLGMD
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEE
LNAGGHLTHGASVNFSGKTYHFVPYGVNSETELLDYDEILKIAKQVQPKLIVAGASAYSR
ECCCCCEECCCEEEECCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEECCHHHHH
LIDFAKFREIADSVGAKLMVDMAHIAGLVATGAHPNPLPYADVVTTTTHKTLRGPRGGMI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEECCCHHHHCCCCCCEE
LTNDEALAKKINSAIFPGTQGGPLEHVIAAKAVAFKEALDPEFTTYIEQVIKNTQAMAEE
EECCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
FAKVEGLRLIAGGSDNHLLNLKVLDLGINGKEAQDLLDSVHITLNKEAIPDETLSPFKTS
HHHHCCEEEEECCCCCEEEEEEEEEECCCCHHHHHHHHHHEEEECCCCCCCCCCCCCHHC
GVRIGAAAITSRGFKEVEAKKVAQLVSEALVNHDNQEKLAEVRKAALELTRQFPL
CEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471