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The map label for this gene is yfiJ

Identifier: 15672579

GI number: 15672579

Start: 587730

End: 588380

Strand: Direct

Name: yfiJ

Synonym: L187716

Alternate gene names: 15672579

Gene position: 587730-588380 (Clockwise)

Preceding gene: 15672578

Following gene: 15672580

Centisome position: 24.84

GC content: 37.33

Gene sequence:

>651_bases
TTGATAAAAGCTGCACAGTCTGGAGCTTCATCTAATTTTCTACAAGCTTATTCAATCATTGAGATAGCGGATAATGATTT
ACGTCGTGAACTGGCTAAGATATCAGGTTCAGATGATTATGTGAATCAAACAGGTGTTTTTTATATATTTGTGGCTGACT
GGTATCGACAAGCTCAGATTTTACAAACGGAAAATAAGGATGTGAAGGCTCTCCAGAATATGGAAGCATTGACGGTTGCC
ATTGTTGATACTACAATTGCTGCACAGAATATGGCGATAGCTGCAGAATCTATGGGGCTTGGAATTTGTTATATTGGTGG
GATTCGTAATGATATTGATAAAGTGGCAGATTTACTGGATTTACCAGAATTGACTGTTCCACTTTTTGGTCTTACAATTG
GGATGCCAAAAACACGAAATGAAGTTAAACCACGTTTACCAAGAGCAAATATTTTGTCAGTAAATGCTTATAATCGTGTG
ACAGTGAGTGATTTACAGGCTTATAATGAACAAACTTCAGATTACTATGCCAATCGGAGTCATCATCAAAAGTCTACTGA
TTGGACGCAGGAAATGAGCAATTTTCTGACAAAGCCACGGCGTGAGGATGTTGCCAAATTTTTGAAAAAGCAAGGATTTA
CATTAAACTGA

Upstream 100 bases:

>100_bases
TGATGAAACAATCATGAATCCAACGATTGAAAAAATGTTGCTCCACGCATCAGTTCGTGATTTTAAAGCAGAGCCTTTGT
CAGCTGATGTCAAGGAGTTG

Downstream 100 bases:

>100_bases
AAAGTTGATTAAGCCTGATTTTATTGGGCTTTTTTGCTATACTTGGAAGAGTAATTTAAAAAGATATTAGAAAAATAATT
AATATTGGAAAGTAGAAAAA

Product: NADPH-flavin oxidoreductase

Products: NA

Alternate protein names: NADPH-dependent FMN reductase; NADPH-dependent nitroreductase; NADPH-dependent oxidoreductase [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MIKAAQSGASSNFLQAYSIIEIADNDLRRELAKISGSDDYVNQTGVFYIFVADWYRQAQILQTENKDVKALQNMEALTVA
IVDTTIAAQNMAIAAESMGLGICYIGGIRNDIDKVADLLDLPELTVPLFGLTIGMPKTRNEVKPRLPRANILSVNAYNRV
TVSDLQAYNEQTSDYYANRSHHQKSTDWTQEMSNFLTKPRREDVAKFLKKQGFTLN

Sequences:

>Translated_216_residues
MIKAAQSGASSNFLQAYSIIEIADNDLRRELAKISGSDDYVNQTGVFYIFVADWYRQAQILQTENKDVKALQNMEALTVA
IVDTTIAAQNMAIAAESMGLGICYIGGIRNDIDKVADLLDLPELTVPLFGLTIGMPKTRNEVKPRLPRANILSVNAYNRV
TVSDLQAYNEQTSDYYANRSHHQKSTDWTQEMSNFLTKPRREDVAKFLKKQGFTLN
>Mature_216_residues
MIKAAQSGASSNFLQAYSIIEIADNDLRRELAKISGSDDYVNQTGVFYIFVADWYRQAQILQTENKDVKALQNMEALTVA
IVDTTIAAQNMAIAAESMGLGICYIGGIRNDIDKVADLLDLPELTVPLFGLTIGMPKTRNEVKPRLPRANILSVNAYNRV
TVSDLQAYNEQTSDYYANRSHHQKSTDWTQEMSNFLTKPRREDVAKFLKKQGFTLN

Specific function: Reduces FMNH(2) to FMN, with NADPH as reductant. It also reduces nitroaromatic compounds, quinones and azo dyes [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flavin oxidoreductase frp family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=213, Percent_Identity=38.9671361502347, Blast_Score=147, Evalue=5e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016446
- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 24219; Mature: 24219

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKAAQSGASSNFLQAYSIIEIADNDLRRELAKISGSDDYVNQTGVFYIFVADWYRQAQI
CCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCEEEEEEEHHHHHHHH
LQTENKDVKALQNMEALTVAIVDTTIAAQNMAIAAESMGLGICYIGGIRNDIDKVADLLD
HHCCCCHHHHHHCCHHHEEEEEHHHHHHHHHHEEHHCCCCEEEEECCCHHHHHHHHHHHC
LPELTVPLFGLTIGMPKTRNEVKPRLPRANILSVNAYNRVTVSDLQAYNEQTSDYYANRS
CCCHHHHHHHHEECCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCC
HHQKSTDWTQEMSNFLTKPRREDVAKFLKKQGFTLN
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIKAAQSGASSNFLQAYSIIEIADNDLRRELAKISGSDDYVNQTGVFYIFVADWYRQAQI
CCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCEEEEEEEHHHHHHHH
LQTENKDVKALQNMEALTVAIVDTTIAAQNMAIAAESMGLGICYIGGIRNDIDKVADLLD
HHCCCCHHHHHHCCHHHEEEEEHHHHHHHHHHEEHHCCCCEEEEECCCHHHHHHHHHHHC
LPELTVPLFGLTIGMPKTRNEVKPRLPRANILSVNAYNRVTVSDLQAYNEQTSDYYANRS
CCCHHHHHHHHEECCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCC
HHQKSTDWTQEMSNFLTKPRREDVAKFLKKQGFTLN
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 9836433 [H]