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The map label for this gene is hemK

Identifier: 15672580

GI number: 15672580

Start: 588486

End: 589298

Strand: Direct

Name: hemK

Synonym: L188472

Alternate gene names: 15672580

Gene position: 588486-589298 (Clockwise)

Preceding gene: 15672579

Following gene: 15672581

Centisome position: 24.88

GC content: 34.81

Gene sequence:

>813_bases
ATGCTTTGGATTGAAGCTGTGCGAACTTTGTCAGCAGATTTAGAAGACCCTTTTGAGTTGGAATTTGTCTGGCGAAATTT
ACATGAACTTAATAAATTATCTTGGTTGAATTTAATGCGAGAAAAAATTACTGACCAAGAATTAAAATTACTGACAGAAG
TGTCAAAACGTCTTCATCAAAATGAGCCTCCACAATATATAGTGGGTTGGGCAGAATTCCGTGATTTGAAATTGAAGGTC
GATGAGCGTGTTTTAATTCCTCGTCCTGAAACTGAAGAATTAGTAGAAATGATTTTGGCTGAAAATGAGAAAGATTCCCT
CAAAATTTTAGATATTGGAACAGGGTCAGGAGCGATTGCCATTTCTCTAGCTCAAGCGCGTGAAAATTGGTCAGTAAAAG
CTTCTGATATTTCTAAAGAGGCACTAACTTTAGCAGCTGAAAATGCTGAAATAAATCAGGCTAATTTGGAATTCATTCAA
TCAGATGTACTAGATAAAATTACTGACAGCTTTGATATTATCGTTTCTAATCCGCCATATATTGCTTTTGATGAAACTTA
CGAAATGGATAATTCTGTCATTAAATATGAACCTGATTTAGCGCTTTTTGCGGAAAATCAGGGATTAGCAATTTATCAAA
AAATTGCTGACCAAGCGGTCAATCATCTGACTGATAATGGAAAAATTTATTTGGAAATCGGCTATAAGCAAGGGCAAGCT
GTGCAAGCTATTTTTCAAGAAAAATTTACTGACAAGCTTGTTAGTATTCATCAGGATATATTTGGAAAAGATCGAATGAT
TAGTGTCAAGTGA

Upstream 100 bases:

>100_bases
TGATTAAGCCTGATTTTATTGGGCTTTTTTGCTATACTTGGAAGAGTAATTTAAAAAGATATTAGAAAAATAATTAATAT
TGGAAAGTAGAAAAAGAAAA

Downstream 100 bases:

>100_bases
TAAAAGCTACTGTTGCTATATTTAAGGAGAAAAATGAATTCTTTAATTTTTGAACAAGTATTTCAGCAAAAAGAAGATTT
TTTACCAATATTATTATTAG

Product: protoporphyrinogen oxidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MLWIEAVRTLSADLEDPFELEFVWRNLHELNKLSWLNLMREKITDQELKLLTEVSKRLHQNEPPQYIVGWAEFRDLKLKV
DERVLIPRPETEELVEMILAENEKDSLKILDIGTGSGAIAISLAQARENWSVKASDISKEALTLAAENAEINQANLEFIQ
SDVLDKITDSFDIIVSNPPYIAFDETYEMDNSVIKYEPDLALFAENQGLAIYQKIADQAVNHLTDNGKIYLEIGYKQGQA
VQAIFQEKFTDKLVSIHQDIFGKDRMISVK

Sequences:

>Translated_270_residues
MLWIEAVRTLSADLEDPFELEFVWRNLHELNKLSWLNLMREKITDQELKLLTEVSKRLHQNEPPQYIVGWAEFRDLKLKV
DERVLIPRPETEELVEMILAENEKDSLKILDIGTGSGAIAISLAQARENWSVKASDISKEALTLAAENAEINQANLEFIQ
SDVLDKITDSFDIIVSNPPYIAFDETYEMDNSVIKYEPDLALFAENQGLAIYQKIADQAVNHLTDNGKIYLEIGYKQGQA
VQAIFQEKFTDKLVSIHQDIFGKDRMISVK
>Mature_270_residues
MLWIEAVRTLSADLEDPFELEFVWRNLHELNKLSWLNLMREKITDQELKLLTEVSKRLHQNEPPQYIVGWAEFRDLKLKV
DERVLIPRPETEELVEMILAENEKDSLKILDIGTGSGAIAISLAQARENWSVKASDISKEALTLAAENAEINQANLEFIQ
SDVLDKITDSFDIIVSNPPYIAFDETYEMDNSVIKYEPDLALFAENQGLAIYQKIADQAVNHLTDNGKIYLEIGYKQGQA
VQAIFQEKFTDKLVSIHQDIFGKDRMISVK

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=251, Percent_Identity=30.6772908366534, Blast_Score=105, Evalue=4e-23,
Organism=Escherichia coli, GI1787463, Length=246, Percent_Identity=34.1463414634146, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI87082085, Length=195, Percent_Identity=33.3333333333333, Blast_Score=102, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324266, Length=161, Percent_Identity=33.5403726708075, Blast_Score=75, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24582226, Length=222, Percent_Identity=30.6306306306306, Blast_Score=111, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 30912; Mature: 30912

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLWIEAVRTLSADLEDPFELEFVWRNLHELNKLSWLNLMREKITDQELKLLTEVSKRLHQ
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHC
NEPPQYIVGWAEFRDLKLKVDERVLIPRPETEELVEMILAENEKDSLKILDIGTGSGAIA
CCCCCEEEEHHHHCEEEEEECCEEECCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCEEE
ISLAQARENWSVKASDISKEALTLAAENAEINQANLEFIQSDVLDKITDSFDIIVSNPPY
EEEEHHCCCCCEEHHHCCHHHHEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCE
IAFDETYEMDNSVIKYEPDLALFAENQGLAIYQKIADQAVNHLTDNGKIYLEIGYKQGQA
EEECCCCCCCCCEEEECCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHH
VQAIFQEKFTDKLVSIHQDIFGKDRMISVK
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEC
>Mature Secondary Structure
MLWIEAVRTLSADLEDPFELEFVWRNLHELNKLSWLNLMREKITDQELKLLTEVSKRLHQ
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHC
NEPPQYIVGWAEFRDLKLKVDERVLIPRPETEELVEMILAENEKDSLKILDIGTGSGAIA
CCCCCEEEEHHHHCEEEEEECCEEECCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCEEE
ISLAQARENWSVKASDISKEALTLAAENAEINQANLEFIQSDVLDKITDSFDIIVSNPPY
EEEEHHCCCCCEEHHHCCHHHHEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCE
IAFDETYEMDNSVIKYEPDLALFAENQGLAIYQKIADQAVNHLTDNGKIYLEIGYKQGQA
EEECCCCCCCCCEEEECCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHH
VQAIFQEKFTDKLVSIHQDIFGKDRMISVK
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]