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The map label for this gene is thiD2

Identifier: 15672469

GI number: 15672469

Start: 485020

End: 485808

Strand: Direct

Name: thiD2

Synonym: L0202

Alternate gene names: 15672469

Gene position: 485020-485808 (Clockwise)

Preceding gene: 15672468

Following gene: 15672470

Centisome position: 20.5

GC content: 36.25

Gene sequence:

>789_bases
ATGCCAAAGAATTTAAATGAAAGCAAAAAAATTCTAACAATTGCAGGTTCAGATATCCTATCCGGAGGCGGGATGCAAGC
TGATTTAGCGACTTTTGCAGCCAATGGGCTTTATGGCTTTTGCGCCTTAACAAGCATTGTAACTGTCAAAGAAGATAAAT
TTTTTGTTCATCCAGTAGAAAAAGAACTCTTTCAAGAAGAGTTGGAAAGTTTAAGCGATGTGGACTTTTCAGCCATAAAA
ATAGGACTATTACCCAATAAAGAAATACTTGAACTGACCAAAAACTGGCTGACAGATCTGTCAGTGAAATCTGATACTCC
AATTATATTAGACCCAGTCATTGTTTTTAAAGAAAATGCTGACTATTCGGTCAATGAAATGCGTGACCTTTTCATCAGCC
AACTCTTTCCTTTGGCGACTGTAATTACCCCGAATCTCAAAGAAGCAGAAATACTGACAGGGCTGTCAGTAAAAAGTAAA
TCAGAGATGAAAGAAGCAGCCAAACTTTTGCATGATTTTGGAGCAAAAAATGTCGTCATCAAAGGAGGAAATCGCTTTGA
CCAAAAACAAGCCATTGACCTTGCTTATGATGGAAAAGCTTTTTATGAATTATCAAGCCCGATTATAGAAAATAATAACA
ACGGAGCAGGTTGTACCTTTGCTTCATCAATTGCGGCCAATATGGCTTATAATCACCCCTTTAAAGATTCAGTCAAGAAT
GCAAAAGACTTCGTTTATCAAGCCATTCAAAATGCAAATGAATATGGTGTTTTTCAAAATAAAAAATAA

Upstream 100 bases:

>100_bases
GACCAACAGCGGCACCTGAGGGTTTATATTTGAAAGAAGTCAAATATGCGGAAAATACTGACATCTAAAAGCTACTGTCA
GTAAAATTAATGGAGAGAAG

Downstream 100 bases:

>100_bases
ATTTCCAAAAGTAAACCAAACTTCTAGGAAAAGTAGGAGATGAAACTCATGGACAACAGAAATATCAAAAAATTAACTTT
ACTTGCGATTTGGACAGCAG

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: PN/PL/PM kinase; Pyridoxal kinase; Pyridoxamine kinase; Vitamin B6 kinase [H]

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MPKNLNESKKILTIAGSDILSGGGMQADLATFAANGLYGFCALTSIVTVKEDKFFVHPVEKELFQEELESLSDVDFSAIK
IGLLPNKEILELTKNWLTDLSVKSDTPIILDPVIVFKENADYSVNEMRDLFISQLFPLATVITPNLKEAEILTGLSVKSK
SEMKEAAKLLHDFGAKNVVIKGGNRFDQKQAIDLAYDGKAFYELSSPIIENNNNGAGCTFASSIAANMAYNHPFKDSVKN
AKDFVYQAIQNANEYGVFQNKK

Sequences:

>Translated_262_residues
MPKNLNESKKILTIAGSDILSGGGMQADLATFAANGLYGFCALTSIVTVKEDKFFVHPVEKELFQEELESLSDVDFSAIK
IGLLPNKEILELTKNWLTDLSVKSDTPIILDPVIVFKENADYSVNEMRDLFISQLFPLATVITPNLKEAEILTGLSVKSK
SEMKEAAKLLHDFGAKNVVIKGGNRFDQKQAIDLAYDGKAFYELSSPIIENNNNGAGCTFASSIAANMAYNHPFKDSVKN
AKDFVYQAIQNANEYGVFQNKK
>Mature_261_residues
PKNLNESKKILTIAGSDILSGGGMQADLATFAANGLYGFCALTSIVTVKEDKFFVHPVEKELFQEELESLSDVDFSAIKI
GLLPNKEILELTKNWLTDLSVKSDTPIILDPVIVFKENADYSVNEMRDLFISQLFPLATVITPNLKEAEILTGLSVKSKS
EMKEAAKLLHDFGAKNVVIKGGNRFDQKQAIDLAYDGKAFYELSSPIIENNNNGAGCTFASSIAANMAYNHPFKDSVKNA
KDFVYQAIQNANEYGVFQNKK

Specific function: Phosphorylates B6 vitamers; functions in a salvage pathway. Uses pyridoxal, pyridoxine, and pyridoxamine as substrates [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=250, Percent_Identity=31.2, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1788758, Length=232, Percent_Identity=25.4310344827586, Blast_Score=62, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6325378, Length=252, Percent_Identity=25.7936507936508, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324997, Length=253, Percent_Identity=26.0869565217391, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324517, Length=256, Percent_Identity=26.953125, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.35 [H]

Molecular weight: Translated: 28845; Mature: 28714

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKNLNESKKILTIAGSDILSGGGMQADLATFAANGLYGFCALTSIVTVKEDKFFVHPVE
CCCCCCCCCEEEEEECCHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHEEECCCEEECHHH
KELFQEELESLSDVDFSAIKIGLLPNKEILELTKNWLTDLSVKSDTPIILDPVIVFKENA
HHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCEEECCEEEEECCC
DYSVNEMRDLFISQLFPLATVITPNLKEAEILTGLSVKSKSEMKEAAKLLHDFGAKNVVI
CCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEE
KGGNRFDQKQAIDLAYDGKAFYELSSPIIENNNNGAGCTFASSIAANMAYNHPFKDSVKN
ECCCCCCCHHHEEEEECCCEEEECCCCEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHH
AKDFVYQAIQNANEYGVFQNKK
HHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
PKNLNESKKILTIAGSDILSGGGMQADLATFAANGLYGFCALTSIVTVKEDKFFVHPVE
CCCCCCCCEEEEEECCHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHEEECCCEEECHHH
KELFQEELESLSDVDFSAIKIGLLPNKEILELTKNWLTDLSVKSDTPIILDPVIVFKENA
HHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCEEECCEEEEECCC
DYSVNEMRDLFISQLFPLATVITPNLKEAEILTGLSVKSKSEMKEAAKLLHDFGAKNVVI
CCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEE
KGGNRFDQKQAIDLAYDGKAFYELSSPIIENNNNGAGCTFASSIAANMAYNHPFKDSVKN
ECCCCCCCHHHEEEEECCCEEEECCCCEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHH
AKDFVYQAIQNANEYGVFQNKK
HHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA