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The map label for this gene is truA

Identifier: 15672468

GI number: 15672468

Start: 484226

End: 484987

Strand: Direct

Name: truA

Synonym: L0331

Alternate gene names: 15672468

Gene position: 484226-484987 (Clockwise)

Preceding gene: 15672466

Following gene: 15672469

Centisome position: 20.47

GC content: 35.96

Gene sequence:

>762_bases
ATGACAAGATACAAAGCCACAATAGCCTATGATGGCACAGATTTTGCAGGTTTTCAAAGCCAAACAAATCAAAGAACAGT
TCAAAAAGAAATTGAAAAAATACTGACGAAGTTAAATTCTTTTGAGCCAGTTATTTTACAAGGCTCAGGCCGAACAGATT
CTGGAGTTCATGCTTTTGGTCAAGTCATTCATTTTGATTTAAAGGGTAAAGTTCGTGATTTGGAACGTCTGCGATTTGGT
TTAGACACTCAAACCCCAGCAGATATTGCAGTAAAAAAAGTGGAACTTGTCCCAGATGATTGGCATGCAAGATATCAAAA
GCATGAAAAAACCTATGAGTATTATCTAGAAAATTCAATTACACGAAGTCCATTTCAAAGACATTCTAAAGCCTATTTTC
GCTATCCACTTAATTTTGAGCGGATGCAAGAAGCAATGTCAAAGTTGGTTGGAGAACATGATTTTACAGGTTTTACAGCA
TCGGGTTCATCAGTTGATGACAAAGTAAGAACGATTTATCAGGCAGAAATTATCCGTCTTGACGAAGAAAATTTCAAATT
TATTTTTCGTGGCAATGGCTTTTTGTATAAACAAGTGCGAAACATGGTTGGAACAGTCATTAAAATTGGTAATGACCGAA
TGCCTGTCAGCCAAATAGATAAAATATTGACCTCTAAAAATAGAGATTTTGCAGGACCAACAGCGGCACCTGAGGGTTTA
TATTTGAAAGAAGTCAAATATGCGGAAAATACTGACATCTAA

Upstream 100 bases:

>100_bases
TTTTTACGAAAAGCAATTTAAAAGAAACATATTAAAAAAGGATAAACCATTATAAAAAAAGAAAATAAAGTCCTAATAAA
AATTGTGCTATAATAGAAAA

Downstream 100 bases:

>100_bases
AAGCTACTGTCAGTAAAATTAATGGAGAGAAGATGCCAAAGAATTTAAATGAAAGCAAAAAAATTCTAACAATTGCAGGT
TCAGATATCCTATCCGGAGG

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MTRYKATIAYDGTDFAGFQSQTNQRTVQKEIEKILTKLNSFEPVILQGSGRTDSGVHAFGQVIHFDLKGKVRDLERLRFG
LDTQTPADIAVKKVELVPDDWHARYQKHEKTYEYYLENSITRSPFQRHSKAYFRYPLNFERMQEAMSKLVGEHDFTGFTA
SGSSVDDKVRTIYQAEIIRLDEENFKFIFRGNGFLYKQVRNMVGTVIKIGNDRMPVSQIDKILTSKNRDFAGPTAAPEGL
YLKEVKYAENTDI

Sequences:

>Translated_253_residues
MTRYKATIAYDGTDFAGFQSQTNQRTVQKEIEKILTKLNSFEPVILQGSGRTDSGVHAFGQVIHFDLKGKVRDLERLRFG
LDTQTPADIAVKKVELVPDDWHARYQKHEKTYEYYLENSITRSPFQRHSKAYFRYPLNFERMQEAMSKLVGEHDFTGFTA
SGSSVDDKVRTIYQAEIIRLDEENFKFIFRGNGFLYKQVRNMVGTVIKIGNDRMPVSQIDKILTSKNRDFAGPTAAPEGL
YLKEVKYAENTDI
>Mature_252_residues
TRYKATIAYDGTDFAGFQSQTNQRTVQKEIEKILTKLNSFEPVILQGSGRTDSGVHAFGQVIHFDLKGKVRDLERLRFGL
DTQTPADIAVKKVELVPDDWHARYQKHEKTYEYYLENSITRSPFQRHSKAYFRYPLNFERMQEAMSKLVGEHDFTGFTAS
GSSVDDKVRTIYQAEIIRLDEENFKFIFRGNGFLYKQVRNMVGTVIKIGNDRMPVSQIDKILTSKNRDFAGPTAAPEGLY
LKEVKYAENTDI

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=276, Percent_Identity=33.695652173913, Blast_Score=114, Evalue=7e-26,
Organism=Homo sapiens, GI199559785, Length=251, Percent_Identity=29.4820717131474, Blast_Score=72, Evalue=4e-13,
Organism=Escherichia coli, GI1788657, Length=246, Percent_Identity=36.1788617886179, Blast_Score=164, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6321112, Length=232, Percent_Identity=28.448275862069, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI116007752, Length=307, Percent_Identity=28.6644951140065, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281362146, Length=264, Percent_Identity=26.5151515151515, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24648455, Length=264, Percent_Identity=26.5151515151515, Blast_Score=71, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_LACLA (Q9CI80)

Other databases:

- EMBL:   AE005176
- PIR:   F86685
- RefSeq:   NP_266642.1
- ProteinModelPortal:   Q9CI80
- SMR:   Q9CI80
- GeneID:   1114104
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0331
- NMPDR:   fig|272623.1.peg.501
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- ProtClustDB:   PRK00021
- BioCyc:   LLAC272623:L0331-MONOMER
- BRENDA:   5.4.99.12
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 29145; Mature: 29014

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: NA

Important sites: ACT_SITE 53-53

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRYKATIAYDGTDFAGFQSQTNQRTVQKEIEKILTKLNSFEPVILQGSGRTDSGVHAFG
CCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHH
QVIHFDLKGKVRDLERLRFGLDTQTPADIAVKKVELVPDDWHARYQKHEKTYEYYLENSI
HHEEEECCCHHHHHHHHHHCCCCCCCHHHEEEEEEECCCHHHHHHHHHHHHHHHHHHCCC
TRSPFQRHSKAYFRYPLNFERMQEAMSKLVGEHDFTGFTASGSSVDDKVRTIYQAEIIRL
CCCHHHHHCCEEEECCCCHHHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHHHEEE
DEENFKFIFRGNGFLYKQVRNMVGTVIKIGNDRMPVSQIDKILTSKNRDFAGPTAAPEGL
CCCCEEEEEECCCCHHHHHHHHHHHHEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
YLKEVKYAENTDI
CCEEEECCCCCCC
>Mature Secondary Structure 
TRYKATIAYDGTDFAGFQSQTNQRTVQKEIEKILTKLNSFEPVILQGSGRTDSGVHAFG
CCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHH
QVIHFDLKGKVRDLERLRFGLDTQTPADIAVKKVELVPDDWHARYQKHEKTYEYYLENSI
HHEEEECCCHHHHHHHHHHCCCCCCCHHHEEEEEEECCCHHHHHHHHHHHHHHHHHHCCC
TRSPFQRHSKAYFRYPLNFERMQEAMSKLVGEHDFTGFTASGSSVDDKVRTIYQAEIIRL
CCCHHHHHCCEEEECCCCHHHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHHHEEE
DEENFKFIFRGNGFLYKQVRNMVGTVIKIGNDRMPVSQIDKILTSKNRDFAGPTAAPEGL
CCCCEEEEEECCCCHHHHHHHHHHHHEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
YLKEVKYAENTDI
CCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471