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The map label for this gene is yecE

Identifier: 15672402

GI number: 15672402

Start: 426638

End: 427666

Strand: Direct

Name: yecE

Synonym: L26628

Alternate gene names: 15672402

Gene position: 426638-427666 (Clockwise)

Preceding gene: 15672401

Following gene: 15672403

Centisome position: 18.04

GC content: 37.03

Gene sequence:

>1029_bases
ATGATGAAGGCGAGATTGATTTACAATCCGACATCGGGTCAAGAAATTATAAAAAAATATATTGCAGATATTTTGGATAA
ATTAGAGCAATATGGTTATGAGGCCAGTGCTTATCAAACCACAGCAGAACAAGATTCCGCAAAAAAAGAGGCGGCAAGAG
CAACAGAAGCTGGCTTTGATTTAATCATTGCGGCTGGTGGTGATGGTACTATTAATGAAGTTGTAGCTGGAATTTCTCCT
TTTGAGAAACGTCCTCAATTAGCTATTGTTCCAACGGGAACGACAAACGACTTTGCTAGGGCTTTAAAAATTCCAAGAGG
AAAACCGCTTGAAGCGATTGAAATTATTGGTAAAAATCAGATTCTAAATATTGATGTCGGTCATGCCGTAATCAGAGAAA
CACAAGATGAACAGTATTTTATTAATATCGCTGCTGGGGGCGGTTTGACAGAACTTACTTACAGTGTTCCCAGTCATTTA
AAAACGGCTTTTGGTTATTTGGCCTATTTAGCAAAGGGTGCAGAACTTTTACCTCGTGTGCGTAAAGCACCGGTTCGTGT
TGTTCATGATGAAGGCGTATTTGAGGGTGATATTTCAATGTTCTTTGCCGCACTTACTAATTCAGTTGGTGGCTTTGAAA
AGATTGCACCTGATGCTAAATTAGATGATGGACTTTTCACACTGATTTTGGTGAAAACAGATAATCTATTTGAACTTATT
GCTTTACTTGCGATTGTAGCTAATGGAAAACATTTGGATGATGTTAATTTGGAATACATTAAAACAAGTAAAATAGAAAT
CGAAGCCTTAGGCGGTCAAAAAATTCTTCTTAATTTAGATGGAGAATATGGTGGAGATGCTCCAGTTCAATTTGATAATC
TCAAAGGTCATTTGGATATGTATGTAAATCTTGATGAAATCAACGATGACCATTACCTTGGAGACGAAGAAACCTTGGCA
TTAGAAGCAGTTGCGCAAAAATTTGCCGAAGAAGCCAATAAAATTAAAGACGAAGATGAAGAAGATTAA

Upstream 100 bases:

>100_bases
TGACAGAAATTTGAACTTTGGTTCAATTGGTGGGGTCACTCTTTTGGGAGTTTGTCAGTAAATTATTTTTAAGAATGGAT
GAGAGAAGGTTTCGGAGAAA

Downstream 100 bases:

>100_bases
AAAGTTACTGACAGATCTGTCAGTAACTTTTTCTGTCAGTAAAATACGACGGCTCGGAGCATTTTAGTTGAGGCTAAGAG
AATTGTCAACTACATTAATA

Product: putative lipid kinase

Products: NA

Alternate protein names: DAG kinase; DAGK [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MMKARLIYNPTSGQEIIKKYIADILDKLEQYGYEASAYQTTAEQDSAKKEAARATEAGFDLIIAAGGDGTINEVVAGISP
FEKRPQLAIVPTGTTNDFARALKIPRGKPLEAIEIIGKNQILNIDVGHAVIRETQDEQYFINIAAGGGLTELTYSVPSHL
KTAFGYLAYLAKGAELLPRVRKAPVRVVHDEGVFEGDISMFFAALTNSVGGFEKIAPDAKLDDGLFTLILVKTDNLFELI
ALLAIVANGKHLDDVNLEYIKTSKIEIEALGGQKILLNLDGEYGGDAPVQFDNLKGHLDMYVNLDEINDDHYLGDEETLA
LEAVAQKFAEEANKIKDEDEED

Sequences:

>Translated_342_residues
MMKARLIYNPTSGQEIIKKYIADILDKLEQYGYEASAYQTTAEQDSAKKEAARATEAGFDLIIAAGGDGTINEVVAGISP
FEKRPQLAIVPTGTTNDFARALKIPRGKPLEAIEIIGKNQILNIDVGHAVIRETQDEQYFINIAAGGGLTELTYSVPSHL
KTAFGYLAYLAKGAELLPRVRKAPVRVVHDEGVFEGDISMFFAALTNSVGGFEKIAPDAKLDDGLFTLILVKTDNLFELI
ALLAIVANGKHLDDVNLEYIKTSKIEIEALGGQKILLNLDGEYGGDAPVQFDNLKGHLDMYVNLDEINDDHYLGDEETLA
LEAVAQKFAEEANKIKDEDEED
>Mature_342_residues
MMKARLIYNPTSGQEIIKKYIADILDKLEQYGYEASAYQTTAEQDSAKKEAARATEAGFDLIIAAGGDGTINEVVAGISP
FEKRPQLAIVPTGTTNDFARALKIPRGKPLEAIEIIGKNQILNIDVGHAVIRETQDEQYFINIAAGGGLTELTYSVPSHL
KTAFGYLAYLAKGAELLPRVRKAPVRVVHDEGVFEGDISMFFAALTNSVGGFEKIAPDAKLDDGLFTLILVKTDNLFELI
ALLAIVANGKHLDDVNLEYIKTSKIEIEALGGQKILLNLDGEYGGDAPVQFDNLKGHLDMYVNLDEINDDHYLGDEETLA
LEAVAQKFAEEANKIKDEDEED

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthet

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI1788402, Length=246, Percent_Identity=29.2682926829268, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017438
- InterPro:   IPR005218
- InterPro:   IPR001206 [H]

Pfam domain/function: PF00781 DAGK_cat [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 37364; Mature: 37364

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKARLIYNPTSGQEIIKKYIADILDKLEQYGYEASAYQTTAEQDSAKKEAARATEAGFD
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHCHHHHHHHHHHHHHHHHCCCE
LIIAAGGDGTINEVVAGISPFEKRPQLAIVPTGTTNDFARALKIPRGKPLEAIEIIGKNQ
EEEEECCCCCHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHCCCCCCCCHHHEEECCCE
ILNIDVGHAVIRETQDEQYFINIAAGGGLTELTYSVPSHLKTAFGYLAYLAKGAELLPRV
EEEEECCHHHHHCCCCCEEEEEEECCCCHHEEEEHHHHHHHHHHHHHHHHHCCHHHHHHH
RKAPVRVVHDEGVFEGDISMFFAALTNSVGGFEKIAPDAKLDDGLFTLILVKTDNLFELI
HCCCEEEEECCCCEEHHHHHHHHHHHHCCCCHHHHCCCCCCCCCEEEEEEEECCCHHHHH
ALLAIVANGKHLDDVNLEYIKTSKIEIEALGGQKILLNLDGEYGGDAPVQFDNLKGHLDM
HHHHHHHCCCCCCCCCEEEEEECEEEEEEECCCEEEEEECCCCCCCCCEEECCCCCEEEE
YVNLDEINDDHYLGDEETLALEAVAQKFAEEANKIKDEDEED
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MMKARLIYNPTSGQEIIKKYIADILDKLEQYGYEASAYQTTAEQDSAKKEAARATEAGFD
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHCHHHHHHHHHHHHHHHHCCCE
LIIAAGGDGTINEVVAGISPFEKRPQLAIVPTGTTNDFARALKIPRGKPLEAIEIIGKNQ
EEEEECCCCCHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHCCCCCCCCHHHEEECCCE
ILNIDVGHAVIRETQDEQYFINIAAGGGLTELTYSVPSHLKTAFGYLAYLAKGAELLPRV
EEEEECCHHHHHCCCCCEEEEEEECCCCHHEEEEHHHHHHHHHHHHHHHHHCCHHHHHHH
RKAPVRVVHDEGVFEGDISMFFAALTNSVGGFEKIAPDAKLDDGLFTLILVKTDNLFELI
HCCCEEEEECCCCEEHHHHHHHHHHHHCCCCHHHHCCCCCCCCCEEEEEEEECCCHHHHH
ALLAIVANGKHLDDVNLEYIKTSKIEIEALGGQKILLNLDGEYGGDAPVQFDNLKGHLDM
HHHHHHHCCCCCCCCCEEEEEECEEEEEEECCCEEEEEECCCCCCCCCEEECCCCCEEEE
YVNLDEINDDHYLGDEETLALEAVAQKFAEEANKIKDEDEED
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]