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The map label for this gene is ligA

Identifier: 15672401

GI number: 15672401

Start: 424521

End: 426485

Strand: Direct

Name: ligA

Synonym: L0304

Alternate gene names: 15672401

Gene position: 424521-426485 (Clockwise)

Preceding gene: 15672400

Following gene: 15672402

Centisome position: 17.95

GC content: 38.98

Gene sequence:

>1965_bases
ATGAATATCGAGTCAAAAATAAAAGAGCTTACGGAAAAATTAAATCAGTATGCTTATGAATATTATACTTTAGATGAGCC
TTCGGTAGAAGACAGTGAGTACGATCGACTCTATCAAGAATTGGTAAAATTGGAGGCTGAAAATCCACAACTAACTAGAG
CGGATTCTCCAACGCACCGAACAGGTGGGGTTATTTTAGATGGATTTGTTAAATTTAGACACCCTTATAATCTCTATAGT
TTAGGGGATGTTTTTTCGCGTGAAGAATTAGCAGTTTGGGAAGAACGAGTCCGCAAGGAAATTGCTAATCCAGAGTATAT
TTGTGAATTAAAAATTGATGGTCTTTCATTGTCTCTTTATTATGAGAATGGTTTGCTTATGACTGCAGCGACTCGGGGCG
ATGGAACAACGGGTGAAAATATCACAGAAAATGTGAAACGGATCAAAGATGTTCCTTTAAAGCTTAAAGAAGCAATTGAT
ATTGTGGTACGTGGTGAAGCTTATCTTCCACGTAAGAATTTTGCTAAACTGAACAAAGAGCGTGAACTTGAAGGAGCAAC
TCCTTTTGCTAATCCAAGAAATGCGGCAGCTGGAACTTTACGTCAACTTGATACAAAAATAGTTGCCAAACGTGGTCTTG
CAACTTTCCTTTATCAAGAAGCAAGTCCAGCAACCAATGACACTCAAGAAGAAGTCTTAGAATATTTTGAAGAACTTGGT
TTCCAAGTCAATCCTGAACGTAAATTTGCTCGAAATATGGATGAAATTTGGGAATTTATTGAAGAGGCTACCCGTCTGCG
TGACGAACTCCCCTATGACATTGACGGGGTTGTTGTAAAAGTTAATAATCTTGCTGAGCAGGAGGAATTAGGTTTTACGG
TTAAGGCACCGCGCTGGGCGATTGCTTACAAATTTCCAGCTGAACAAGCAGAAACAGAAATTTTGTCTGTTGACTGGACT
GTCGGTCGGACAGGAGTTGTCACCCCAACAGCAAATATGACCCCGGTTCTTTTAGCACAGACAACGGTTGCTCGAGCAAC
TTTGCACAATGTGGACTATATTGAAGAAAAAGATATCCGTATTGGTGATCATGTCTTGATTTATAAGGCAGGGGATATTA
TTCCGAAGGTTGGGAAAGTACTTTTGGACAAACGCCCAGAAGGTCTTGAAGGCTTAGAAATTCCAACGAAATGTCCAGAG
TGTGGTTCGGAGTTAATTCATTTTGAAGATGAAGTGGCTTTACGCTGTGTTAACCCCTTATGTCCGGCGCAAATTCGGGA
AAAACTAATTCATTTTGCTAGTCGTGATGCTATGAATATTGTGGGCTTAGGCCCGTCAGTCATTTCACAACTTTTTGATA
AAAAATTGGTGGCTGATGTTGCTGATTTATATCAATTGACGATTGAAGATTTACTGACATTAGATAAGGTCAAAGAAACA
TTGGCTCAAAAAATTGTGTCAGCAATTGCGCAGAGTCGTGAAAATTCGGCGGAAAAATTATTATTTGGTTTAGGAATTCG
TCATGTTGGTGGTAAAGCGGCTAAATTACTTTTGGAACGTTTTGCTAATCTTCGAGCGCTTTCTAAAGCAACAGAAGAAG
AAATTTCTGAAATTCCATCTTTAGGTGGGGTCATTGCTACGGCTGTCGTTTCTTATTTTGAAACTGACGGAGCAAAAATT
TTACTGGATGAACTTGAAAATGCTGGTGTAAATTTTGATTATTTAGGGGCTGTTAATATTGAAGGAATATTATCAGGTAA
GACAGTTGTCTTAACTGGTAAATTGACGACTTTGAAGCGCTCAGAAGCAAAAGAAAAATTGGAAGCGTTGGGAGCAAATG
TTTCTGGTTCTGTTTCTAAGAAAACTGATTTGGTAGTCGCCGGAGAAGAAGCAGGTAGCAAACTGACAAAAGCTCAAGAC
TTAGGAATTGAAATCTGGTCAGAGCAAGATTTGCTTGATTTATAA

Upstream 100 bases:

>100_bases
TTTTTTTGTTTTAAATTACATCATTTTTTTAATAGAAATTTGCCTTTTTGATGTGCTTCGGTAATCATAATGACTGGTCT
TTTGTGTTAAAATAGGAGCT

Downstream 100 bases:

>100_bases
ACTAATTTGTATTGAATATTACTGACAGACTTTAGTGAGAGCGTAAATTTACTGACAGAAATTTGAACTTTGGTTCAATT
GGTGGGGTCACTCTTTTGGG

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 654; Mature: 654

Protein sequence:

>654_residues
MNIESKIKELTEKLNQYAYEYYTLDEPSVEDSEYDRLYQELVKLEAENPQLTRADSPTHRTGGVILDGFVKFRHPYNLYS
LGDVFSREELAVWEERVRKEIANPEYICELKIDGLSLSLYYENGLLMTAATRGDGTTGENITENVKRIKDVPLKLKEAID
IVVRGEAYLPRKNFAKLNKERELEGATPFANPRNAAAGTLRQLDTKIVAKRGLATFLYQEASPATNDTQEEVLEYFEELG
FQVNPERKFARNMDEIWEFIEEATRLRDELPYDIDGVVVKVNNLAEQEELGFTVKAPRWAIAYKFPAEQAETEILSVDWT
VGRTGVVTPTANMTPVLLAQTTVARATLHNVDYIEEKDIRIGDHVLIYKAGDIIPKVGKVLLDKRPEGLEGLEIPTKCPE
CGSELIHFEDEVALRCVNPLCPAQIREKLIHFASRDAMNIVGLGPSVISQLFDKKLVADVADLYQLTIEDLLTLDKVKET
LAQKIVSAIAQSRENSAEKLLFGLGIRHVGGKAAKLLLERFANLRALSKATEEEISEIPSLGGVIATAVVSYFETDGAKI
LLDELENAGVNFDYLGAVNIEGILSGKTVVLTGKLTTLKRSEAKEKLEALGANVSGSVSKKTDLVVAGEEAGSKLTKAQD
LGIEIWSEQDLLDL

Sequences:

>Translated_654_residues
MNIESKIKELTEKLNQYAYEYYTLDEPSVEDSEYDRLYQELVKLEAENPQLTRADSPTHRTGGVILDGFVKFRHPYNLYS
LGDVFSREELAVWEERVRKEIANPEYICELKIDGLSLSLYYENGLLMTAATRGDGTTGENITENVKRIKDVPLKLKEAID
IVVRGEAYLPRKNFAKLNKERELEGATPFANPRNAAAGTLRQLDTKIVAKRGLATFLYQEASPATNDTQEEVLEYFEELG
FQVNPERKFARNMDEIWEFIEEATRLRDELPYDIDGVVVKVNNLAEQEELGFTVKAPRWAIAYKFPAEQAETEILSVDWT
VGRTGVVTPTANMTPVLLAQTTVARATLHNVDYIEEKDIRIGDHVLIYKAGDIIPKVGKVLLDKRPEGLEGLEIPTKCPE
CGSELIHFEDEVALRCVNPLCPAQIREKLIHFASRDAMNIVGLGPSVISQLFDKKLVADVADLYQLTIEDLLTLDKVKET
LAQKIVSAIAQSRENSAEKLLFGLGIRHVGGKAAKLLLERFANLRALSKATEEEISEIPSLGGVIATAVVSYFETDGAKI
LLDELENAGVNFDYLGAVNIEGILSGKTVVLTGKLTTLKRSEAKEKLEALGANVSGSVSKKTDLVVAGEEAGSKLTKAQD
LGIEIWSEQDLLDL
>Mature_654_residues
MNIESKIKELTEKLNQYAYEYYTLDEPSVEDSEYDRLYQELVKLEAENPQLTRADSPTHRTGGVILDGFVKFRHPYNLYS
LGDVFSREELAVWEERVRKEIANPEYICELKIDGLSLSLYYENGLLMTAATRGDGTTGENITENVKRIKDVPLKLKEAID
IVVRGEAYLPRKNFAKLNKERELEGATPFANPRNAAAGTLRQLDTKIVAKRGLATFLYQEASPATNDTQEEVLEYFEELG
FQVNPERKFARNMDEIWEFIEEATRLRDELPYDIDGVVVKVNNLAEQEELGFTVKAPRWAIAYKFPAEQAETEILSVDWT
VGRTGVVTPTANMTPVLLAQTTVARATLHNVDYIEEKDIRIGDHVLIYKAGDIIPKVGKVLLDKRPEGLEGLEIPTKCPE
CGSELIHFEDEVALRCVNPLCPAQIREKLIHFASRDAMNIVGLGPSVISQLFDKKLVADVADLYQLTIEDLLTLDKVKET
LAQKIVSAIAQSRENSAEKLLFGLGIRHVGGKAAKLLLERFANLRALSKATEEEISEIPSLGGVIATAVVSYFETDGAKI
LLDELENAGVNFDYLGAVNIEGILSGKTVVLTGKLTTLKRSEAKEKLEALGANVSGSVSKKTDLVVAGEEAGSKLTKAQD
LGIEIWSEQDLLDL

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=666, Percent_Identity=44.7447447447448, Blast_Score=552, Evalue=1e-158,
Organism=Escherichia coli, GI87082305, Length=568, Percent_Identity=22.887323943662, Blast_Score=117, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_LACLA (Q9CIE4)

Other databases:

- EMBL:   AE005176
- PIR:   C86677
- RefSeq:   NP_266575.1
- HSSP:   O87703
- ProteinModelPortal:   Q9CIE4
- GeneID:   1114031
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0304
- NMPDR:   fig|272623.1.peg.428
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   PRK07956
- BioCyc:   LLAC272623:L0304-MONOMER
- BRENDA:   6.5.1.2
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 72739; Mature: 72739

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 111-111 BINDING 109-109 BINDING 132-132 BINDING 166-166 BINDING 280-280 BINDING 304-304

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIESKIKELTEKLNQYAYEYYTLDEPSVEDSEYDRLYQELVKLEAENPQLTRADSPTHR
CCHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
TGGVILDGFVKFRHPYNLYSLGDVFSREELAVWEERVRKEIANPEYICELKIDGLSLSLY
CCCEEEECHHHHCCCCCCHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEEE
YENGLLMTAATRGDGTTGENITENVKRIKDVPLKLKEAIDIVVRGEAYLPRKNFAKLNKE
EECCEEEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHEEEEEECCCCCCCHHHHHCCCH
RELEGATPFANPRNAAAGTLRQLDTKIVAKRGLATFLYQEASPATNDTQEEVLEYFEELG
HCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCC
FQVNPERKFARNMDEIWEFIEEATRLRDELPYDIDGVVVKVNNLAEQEELGFTVKAPRWA
CEECCHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHCCEEEECCCEE
IAYKFPAEQAETEILSVDWTVGRTGVVTPTANMTPVLLAQTTVARATLHNVDYIEEKDIR
EEEECCCHHHCCEEEEEEEECCCCEEECCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCE
IGDHVLIYKAGDIIPKVGKVLLDKRPEGLEGLEIPTKCPECGSELIHFEDEVALRCVNPL
ECCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHCCCC
CPAQIREKLIHFASRDAMNIVGLGPSVISQLFDKKLVADVADLYQLTIEDLLTLDKVKET
CHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAQKIVSAIAQSRENSAEKLLFGLGIRHVGGKAAKLLLERFANLRALSKATEEEISEIPS
HHHHHHHHHHHHCCCHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LGGVIATAVVSYFETDGAKILLDELENAGVNFDYLGAVNIEGILSGKTVVLTGKLTTLKR
CCHHHHHHHHHHHHCCCCEEEHHHHHCCCCCEEEEECEEECEEECCCEEEEECCCHHHHH
SEAKEKLEALGANVSGSVSKKTDLVVAGEEAGSKLTKAQDLGIEIWSEQDLLDL
HHHHHHHHHHCCCCCCCCCCCCEEEEECCHHCCHHHHHHHCCEEEECCCCCCCC
>Mature Secondary Structure
MNIESKIKELTEKLNQYAYEYYTLDEPSVEDSEYDRLYQELVKLEAENPQLTRADSPTHR
CCHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
TGGVILDGFVKFRHPYNLYSLGDVFSREELAVWEERVRKEIANPEYICELKIDGLSLSLY
CCCEEEECHHHHCCCCCCHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEEE
YENGLLMTAATRGDGTTGENITENVKRIKDVPLKLKEAIDIVVRGEAYLPRKNFAKLNKE
EECCEEEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHEEEEEECCCCCCCHHHHHCCCH
RELEGATPFANPRNAAAGTLRQLDTKIVAKRGLATFLYQEASPATNDTQEEVLEYFEELG
HCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCC
FQVNPERKFARNMDEIWEFIEEATRLRDELPYDIDGVVVKVNNLAEQEELGFTVKAPRWA
CEECCHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHCCEEEECCCEE
IAYKFPAEQAETEILSVDWTVGRTGVVTPTANMTPVLLAQTTVARATLHNVDYIEEKDIR
EEEECCCHHHCCEEEEEEEECCCCEEECCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCE
IGDHVLIYKAGDIIPKVGKVLLDKRPEGLEGLEIPTKCPECGSELIHFEDEVALRCVNPL
ECCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHCCCC
CPAQIREKLIHFASRDAMNIVGLGPSVISQLFDKKLVADVADLYQLTIEDLLTLDKVKET
CHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAQKIVSAIAQSRENSAEKLLFGLGIRHVGGKAAKLLLERFANLRALSKATEEEISEIPS
HHHHHHHHHHHHCCCHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LGGVIATAVVSYFETDGAKILLDELENAGVNFDYLGAVNIEGILSGKTVVLTGKLTTLKR
CCHHHHHHHHHHHHCCCCEEEHHHHHCCCCCEEEEECEEECEEECCCEEEEECCCHHHHH
SEAKEKLEALGANVSGSVSKKTDLVVAGEEAGSKLTKAQDLGIEIWSEQDLLDL
HHHHHHHHHHCCCCCCCCCCCCEEEEECCHHCCHHHHHHHCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471