Click here to switch to the map view.

The map label for this gene is rlrG

Identifier: 15672356

GI number: 15672356

Start: 377467

End: 378285

Strand: Reverse

Name: rlrG

Synonym: L0220

Alternate gene names: 15672356

Gene position: 378285-377467 (Counterclockwise)

Preceding gene: 15672358

Following gene: 15672355

Centisome position: 15.99

GC content: 32.72

Gene sequence:

>819_bases
ATGTTCGCTTTACTCGAAACTTTTATTGCTGTGTTTGAAACCAAAAGCTTTACCAGAGCTGCTAATCAATGTTTTATTTC
ACAGCCAACCGCAACTGTTAGAATTAAAAAATTGGAGGAAGAACTAAAAGTTCACCTTTTTTCAAGAGGGCAACACCAAG
AAGTAATTCCCACAGAAAGTGCCCACCTTCTTTATCCTAAGGCTTTAAAAACGCTCAATGATTGGAATGAATTACAATTT
TCCCTAAAAAATCAGACACCTAGTCGTAAACCTTTTAAAATTGCTGCTTCCCATAGTTCAGCAACTACTGTTTTACCTTT
GATTTTTAAAAATTTAATTCCTGAACTTGAACAACTTAACATTGAATTAATGATGCTTGATTCTCAAGAGGTTTTTGATT
TAATTCGTAATCATGAAATTCATTTTGGAATTATTGAAAAACCGATGATGGATGAAACCATCGAAACTTTCCCACTTTTT
AAAGATGAACTCGTTCTTGCTGGCAATCCAGAAAGTGACATCTTTTTTATTCGCGAAGCAGGGTCTGGTGTGGCTCATTA
TACACATAATTATCTTAAAGAAAATAATCTTAATCCAAAGAATTTGGTCAGCGTCAATAACAATGATATGATTGTCAGTC
AACTTAAGGCTGGCATTGGCCTGTCATTGATTTCAAAACGATTTATTAATGAAAATATTGCTTTTCAAAATCTTGGCGAA
CGTTATCAACGCATTTTTTATGGTCTTTCTTTTTTGTCTGAACAAGACAAATTGCTCAAAACTGTGATTAAAAAAATCAA
AAATTTAAGTGAGTTTTAA

Upstream 100 bases:

>100_bases
TTTAATTGTCTAGCATCAATTAGTTTACACAATCTCCTTTATTTTGTAAAATAAATTATTATCATTTTTAAAATTACTTT
TATTAATTAAAGGAAGATTT

Downstream 100 bases:

>100_bases
AAAATTTACTGACAGAAATTCTTTAAGGTCATTCATCATTTTCTAAATTTCTGTCAGTCATTTTATCAGCATTTTTTACT
GATAGTAAAAAAATCTTCTC

Product: LysR family transcription regulator

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MFALLETFIAVFETKSFTRAANQCFISQPTATVRIKKLEEELKVHLFSRGQHQEVIPTESAHLLYPKALKTLNDWNELQF
SLKNQTPSRKPFKIAASHSSATTVLPLIFKNLIPELEQLNIELMMLDSQEVFDLIRNHEIHFGIIEKPMMDETIETFPLF
KDELVLAGNPESDIFFIREAGSGVAHYTHNYLKENNLNPKNLVSVNNNDMIVSQLKAGIGLSLISKRFINENIAFQNLGE
RYQRIFYGLSFLSEQDKLLKTVIKKIKNLSEF

Sequences:

>Translated_272_residues
MFALLETFIAVFETKSFTRAANQCFISQPTATVRIKKLEEELKVHLFSRGQHQEVIPTESAHLLYPKALKTLNDWNELQF
SLKNQTPSRKPFKIAASHSSATTVLPLIFKNLIPELEQLNIELMMLDSQEVFDLIRNHEIHFGIIEKPMMDETIETFPLF
KDELVLAGNPESDIFFIREAGSGVAHYTHNYLKENNLNPKNLVSVNNNDMIVSQLKAGIGLSLISKRFINENIAFQNLGE
RYQRIFYGLSFLSEQDKLLKTVIKKIKNLSEF
>Mature_272_residues
MFALLETFIAVFETKSFTRAANQCFISQPTATVRIKKLEEELKVHLFSRGQHQEVIPTESAHLLYPKALKTLNDWNELQF
SLKNQTPSRKPFKIAASHSSATTVLPLIFKNLIPELEQLNIELMMLDSQEVFDLIRNHEIHFGIIEKPMMDETIETFPLF
KDELVLAGNPESDIFFIREAGSGVAHYTHNYLKENNLNPKNLVSVNNNDMIVSQLKAGIGLSLISKRFINENIAFQNLGE
RYQRIFYGLSFLSEQDKLLKTVIKKIKNLSEF

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=252, Percent_Identity=28.1746031746032, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31246; Mature: 31246

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFALLETFIAVFETKSFTRAANQCFISQPTATVRIKKLEEELKVHLFSRGQHQEVIPTES
CHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
AHLLYPKALKTLNDWNELQFSLKNQTPSRKPFKIAASHSSATTVLPLIFKNLIPELEQLN
CCEECHHHHHHHCCHHHHEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCHHHHCC
IELMMLDSQEVFDLIRNHEIHFGIIEKPMMDETIETFPLFKDELVLAGNPESDIFFIREA
EEEEEECCHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCCCCEEEECCCCCCEEEEEEC
GSGVAHYTHNYLKENNLNPKNLVSVNNNDMIVSQLKAGIGLSLISKRFINENIAFQNLGE
CCCHHHHHHHHHHCCCCCHHHEEEECCCCHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHH
RYQRIFYGLSFLSEQDKLLKTVIKKIKNLSEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFALLETFIAVFETKSFTRAANQCFISQPTATVRIKKLEEELKVHLFSRGQHQEVIPTES
CHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
AHLLYPKALKTLNDWNELQFSLKNQTPSRKPFKIAASHSSATTVLPLIFKNLIPELEQLN
CCEECHHHHHHHCCHHHHEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCHHHHCC
IELMMLDSQEVFDLIRNHEIHFGIIEKPMMDETIETFPLFKDELVLAGNPESDIFFIREA
EEEEEECCHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCCCCEEEECCCCCCEEEEEEC
GSGVAHYTHNYLKENNLNPKNLVSVNNNDMIVSQLKAGIGLSLISKRFINENIAFQNLGE
CCCHHHHHHHHHHCCCCCHHHEEEECCCCHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHH
RYQRIFYGLSFLSEQDKLLKTVIKKIKNLSEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]