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The map label for this gene is asnB

Identifier: 15672334

GI number: 15672334

Start: 356055

End: 357647

Strand: Direct

Name: asnB

Synonym: L00396

Alternate gene names: 15672334

Gene position: 356055-357647 (Clockwise)

Preceding gene: 15672333

Following gene: 15672335

Centisome position: 15.05

GC content: 37.66

Gene sequence:

>1593_bases
ATGTGCGGTTTTTTATTTATGGAAACATCTGATTTAACGATAGAAGAGTTTAAAAGTAATTTGGAATTGATTGAGCATCG
AGGACCAGATGACCAACAGCATGTTCGTGATACTTTGGGAAATGATTTTGGTTTTTGCCGACTTTCAATCATGGATTTAT
CAGACTTGGGACGTCAACCCTTTGGTTTAAATGGCAAAAGAGTTATGTGTAATGGTGAAATCTATAACTTTTTAAGTCTC
CGTAAGTTCTTGGAAACAAAAGGTTATAAATTTACCGGAGATTCTGACTGTGAGGTTCTTTTACCACTTTTTGAAACTAC
TGGAATCGAAACAATGGTAAAAATGCTTGATGCTGAATTTGCTTTTGTCTTGGTTGATGAAAACACTGCTGAAATTTTTG
CCGCTCGTGACCCATTTGGGATTCGTCCACTTTTTTATGGTTATAATAAAGTAACTGGCAAAATTTCGTTTTCATCCGAA
GCCAAAGCACTAATTTCAACATGTAATGATGTGACACCATTTCCTCCAGGTTACTACTATGCCAATGGTGAATTTCATGT
TTTTAATGACATTGCTGAGGTTTCAACAATTGTTGAAGAACCTTTGGCTGAAATTTCAGGACATATTCGTCGCAAATTGG
AACGTGCAGTCAAAAAACGTCTTCATGCAGATGCTCCAATGGGCTATCTCTTGTCAGGTGGTTTAGATTCATCATTGGTT
TGTGCGATTGCAGCTAAGGAATTAGAAAGTCCAATCAAAACCTTTGCTATTGGAATGGAAACCGATCCGATTGATTTGAA
ATATGCTCGTGAAGTGGCAGATTTCTTACGAACTGACCATACCGAAATTCTGATGACAAAAGAAGATGTACTTTCAGCAG
TCCGTGAAGTGATTTGGCACTTAGAAACATGGGATATCACAACTATTCGGGCTTCTATTGGGATGTATTTAATCTGTAAA
TATATTCATGAGAATACAGAACTGAAAGTTTTGATGACTGGTGAAGTTTCTGATGAAATGTTTGGTTATAAATATACTGA
CTTTGCTCCATCAGCGGGTGCCTTTCAAAAAGAAGCTCAAAAACGAGTGCATGAACTTTATATGTATGACGTCCTGCGGG
CTGACCGCTGTTTAGCGGCTCATTCTCTTGAAGCGCGTGTTCCTTTTGCTGATTTGGATTTTGCGGCCTATGTCATGTCT
ATTAATCCCAGCAGAAAATTGAATACTTATGGCAAAGGGAAATATCTCTTGCGTCATGCTTTTGAAGGGACTGAACTATT
GCCAGATGAGATTTTATTCAGAGAAAAAGCTGCTTTTTCTGACGCTGTTGGTCACTCAATGGTTGACTATTTAAAAGAAT
TTGCTGACGAAAAATATTCTGATGAAGACTTAGCAAAGGCCAGTGAAAAGTATCCAAAACATACACCATTTACCAAAGAA
TCATTGATGTATCGTGAGATTTTTGAAGAATTTTATCCAGGATATGAAGATTGGATTCTTGATTTTTGGATGCCAAATCG
CGATTGGGTTGGGGCAGAAGTTACTGACCCATCAGCTCGCGTCTTATCAAATTATGGTGATTCTGGAAAATAA

Upstream 100 bases:

>100_bases
GTAATGAAAATAGCGAATTATCGCTATTTTTGTTTTATTATGAAATTTCCAGACAATTGTGATATAATATTGAAAATTAT
AATTATTATTGGGGGAAATT

Downstream 100 bases:

>100_bases
ATTTTGAAATCTTTATCATAAAAAAATGAATGAGAAATGTTAAAATAGTCACTGATGAAACTTATCTAATGATAATTGTC
AGTGATTTTTTTATATTTAA

Product: asparagine synthetase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MCGFLFMETSDLTIEEFKSNLELIEHRGPDDQQHVRDTLGNDFGFCRLSIMDLSDLGRQPFGLNGKRVMCNGEIYNFLSL
RKFLETKGYKFTGDSDCEVLLPLFETTGIETMVKMLDAEFAFVLVDENTAEIFAARDPFGIRPLFYGYNKVTGKISFSSE
AKALISTCNDVTPFPPGYYYANGEFHVFNDIAEVSTIVEEPLAEISGHIRRKLERAVKKRLHADAPMGYLLSGGLDSSLV
CAIAAKELESPIKTFAIGMETDPIDLKYAREVADFLRTDHTEILMTKEDVLSAVREVIWHLETWDITTIRASIGMYLICK
YIHENTELKVLMTGEVSDEMFGYKYTDFAPSAGAFQKEAQKRVHELYMYDVLRADRCLAAHSLEARVPFADLDFAAYVMS
INPSRKLNTYGKGKYLLRHAFEGTELLPDEILFREKAAFSDAVGHSMVDYLKEFADEKYSDEDLAKASEKYPKHTPFTKE
SLMYREIFEEFYPGYEDWILDFWMPNRDWVGAEVTDPSARVLSNYGDSGK

Sequences:

>Translated_530_residues
MCGFLFMETSDLTIEEFKSNLELIEHRGPDDQQHVRDTLGNDFGFCRLSIMDLSDLGRQPFGLNGKRVMCNGEIYNFLSL
RKFLETKGYKFTGDSDCEVLLPLFETTGIETMVKMLDAEFAFVLVDENTAEIFAARDPFGIRPLFYGYNKVTGKISFSSE
AKALISTCNDVTPFPPGYYYANGEFHVFNDIAEVSTIVEEPLAEISGHIRRKLERAVKKRLHADAPMGYLLSGGLDSSLV
CAIAAKELESPIKTFAIGMETDPIDLKYAREVADFLRTDHTEILMTKEDVLSAVREVIWHLETWDITTIRASIGMYLICK
YIHENTELKVLMTGEVSDEMFGYKYTDFAPSAGAFQKEAQKRVHELYMYDVLRADRCLAAHSLEARVPFADLDFAAYVMS
INPSRKLNTYGKGKYLLRHAFEGTELLPDEILFREKAAFSDAVGHSMVDYLKEFADEKYSDEDLAKASEKYPKHTPFTKE
SLMYREIFEEFYPGYEDWILDFWMPNRDWVGAEVTDPSARVLSNYGDSGK
>Mature_530_residues
MCGFLFMETSDLTIEEFKSNLELIEHRGPDDQQHVRDTLGNDFGFCRLSIMDLSDLGRQPFGLNGKRVMCNGEIYNFLSL
RKFLETKGYKFTGDSDCEVLLPLFETTGIETMVKMLDAEFAFVLVDENTAEIFAARDPFGIRPLFYGYNKVTGKISFSSE
AKALISTCNDVTPFPPGYYYANGEFHVFNDIAEVSTIVEEPLAEISGHIRRKLERAVKKRLHADAPMGYLLSGGLDSSLV
CAIAAKELESPIKTFAIGMETDPIDLKYAREVADFLRTDHTEILMTKEDVLSAVREVIWHLETWDITTIRASIGMYLICK
YIHENTELKVLMTGEVSDEMFGYKYTDFAPSAGAFQKEAQKRVHELYMYDVLRADRCLAAHSLEARVPFADLDFAAYVMS
INPSRKLNTYGKGKYLLRHAFEGTELLPDEILFREKAAFSDAVGHSMVDYLKEFADEKYSDEDLAKASEKYPKHTPFTKE
SLMYREIFEEFYPGYEDWILDFWMPNRDWVGAEVTDPSARVLSNYGDSGK

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=570, Percent_Identity=40.3508771929825, Blast_Score=354, Evalue=1e-97,
Organism=Homo sapiens, GI168229252, Length=570, Percent_Identity=40.3508771929825, Blast_Score=354, Evalue=1e-97,
Organism=Homo sapiens, GI168229250, Length=570, Percent_Identity=40.3508771929825, Blast_Score=354, Evalue=1e-97,
Organism=Homo sapiens, GI296010848, Length=547, Percent_Identity=41.1334552102377, Blast_Score=352, Evalue=5e-97,
Organism=Homo sapiens, GI296010852, Length=477, Percent_Identity=42.1383647798742, Blast_Score=330, Evalue=2e-90,
Organism=Homo sapiens, GI296010850, Length=477, Percent_Identity=42.1383647798742, Blast_Score=330, Evalue=2e-90,
Organism=Escherichia coli, GI1786889, Length=518, Percent_Identity=38.8030888030888, Blast_Score=300, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI25147557, Length=518, Percent_Identity=38.2239382239382, Blast_Score=301, Evalue=7e-82,
Organism=Caenorhabditis elegans, GI71993933, Length=514, Percent_Identity=38.3268482490272, Blast_Score=299, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI17560178, Length=504, Percent_Identity=36.3095238095238, Blast_Score=293, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI25147560, Length=276, Percent_Identity=36.231884057971, Blast_Score=142, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6325403, Length=577, Percent_Identity=37.6083188908146, Blast_Score=306, Evalue=7e-84,
Organism=Saccharomyces cerevisiae, GI6321563, Length=575, Percent_Identity=38.6086956521739, Blast_Score=306, Evalue=7e-84,
Organism=Drosophila melanogaster, GI45553209, Length=519, Percent_Identity=38.728323699422, Blast_Score=315, Evalue=6e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 60384; Mature: 60384

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGFLFMETSDLTIEEFKSNLELIEHRGPDDQQHVRDTLGNDFGFCRLSIMDLSDLGRQP
CCCEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEEEEHHHCCCCC
FGLNGKRVMCNGEIYNFLSLRKFLETKGYKFTGDSDCEVLLPLFETTGIETMVKMLDAEF
CCCCCCEEEECCHHHHHHHHHHHHHHCCCEECCCCCCEEEEHHHHHCCHHHHHHHHCCCE
AFVLVDENTAEIFAARDPFGIRPLFYGYNKVTGKISFSSEAKALISTCNDVTPFPPGYYY
EEEEEECCCEEEEEECCCCCCCHHEECCCEEEEEEEECHHHHHHHHHHCCCCCCCCCEEE
ANGEFHVFNDIAEVSTIVEEPLAEISGHIRRKLERAVKKRLHADAPMGYLLSGGLDSSLV
ECCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCHHH
CAIAAKELESPIKTFAIGMETDPIDLKYAREVADFLRTDHTEILMTKEDVLSAVREVIWH
HHHHHHHHHCCHHHEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHH
LETWDITTIRASIGMYLICKYIHENTELKVLMTGEVSDEMFGYKYTDFAPSAGAFQKEAQ
CCCCCEEHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHCCCEECCCCCCCCHHHHHHH
KRVHELYMYDVLRADRCLAAHSLEARVPFADLDFAAYVMSINPSRKLNTYGKGKYLLRHA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHH
FEGTELLPDEILFREKAAFSDAVGHSMVDYLKEFADEKYSDEDLAKASEKYPKHTPFTKE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCHH
SLMYREIFEEFYPGYEDWILDFWMPNRDWVGAEVTDPSARVLSNYGDSGK
HHHHHHHHHHHCCCHHHHEEEEECCCCCCCCCCCCCCHHHHHHCCCCCCC
>Mature Secondary Structure
MCGFLFMETSDLTIEEFKSNLELIEHRGPDDQQHVRDTLGNDFGFCRLSIMDLSDLGRQP
CCCEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEEEEHHHCCCCC
FGLNGKRVMCNGEIYNFLSLRKFLETKGYKFTGDSDCEVLLPLFETTGIETMVKMLDAEF
CCCCCCEEEECCHHHHHHHHHHHHHHCCCEECCCCCCEEEEHHHHHCCHHHHHHHHCCCE
AFVLVDENTAEIFAARDPFGIRPLFYGYNKVTGKISFSSEAKALISTCNDVTPFPPGYYY
EEEEEECCCEEEEEECCCCCCCHHEECCCEEEEEEEECHHHHHHHHHHCCCCCCCCCEEE
ANGEFHVFNDIAEVSTIVEEPLAEISGHIRRKLERAVKKRLHADAPMGYLLSGGLDSSLV
ECCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCHHH
CAIAAKELESPIKTFAIGMETDPIDLKYAREVADFLRTDHTEILMTKEDVLSAVREVIWH
HHHHHHHHHCCHHHEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHH
LETWDITTIRASIGMYLICKYIHENTELKVLMTGEVSDEMFGYKYTDFAPSAGAFQKEAQ
CCCCCEEHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHCCCEECCCCCCCCHHHHHHH
KRVHELYMYDVLRADRCLAAHSLEARVPFADLDFAAYVMSINPSRKLNTYGKGKYLLRHA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHH
FEGTELLPDEILFREKAAFSDAVGHSMVDYLKEFADEKYSDEDLAKASEKYPKHTPFTKE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCHH
SLMYREIFEEFYPGYEDWILDFWMPNRDWVGAEVTDPSARVLSNYGDSGK
HHHHHHHHHHHCCCHHHHEEEEECCCCCCCCCCCCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1973930; 8905232; 9278503; 10587437 [H]