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The map label for this gene is optS

Identifier: 15672325

GI number: 15672325

Start: 343342

End: 344994

Strand: Direct

Name: optS

Synonym: L143312

Alternate gene names: 15672325

Gene position: 343342-344994 (Clockwise)

Preceding gene: 15672324

Following gene: 15672326

Centisome position: 14.51

GC content: 38.35

Gene sequence:

>1653_bases
ATGAAACAAGGAAAAATTATTGGACTCACCAGCCTTATTGCCCTGTCAGGAATTATTTTAGTGGCCTGTGGCACAAAAAC
AAGTGAGCAAAAAAACATCAAATTTTCAATTCCCACAGATGTGGCATCACTTGATACTACAATTTTAACTGACCAGTATT
CTTATGATGTTGCTGGAAATGTGGAAGAAGGATTGACACGCGTTGACTCTAAAGGAAATGCTGCCTTAGCACTTGCAAAA
TCAATTGACGTTTCAAAAGACGGCTTGACCTACACGGTTACTCTTAGAGATAATTTGAAATGGTCAAACGGTGACAGGTT
GACTGCTAAAGATTTTGTTTATTCATGGAAAAGAGCAGTGGACCCTAAAACTGGTTCAGAATATGCCTATTTGATGGGGG
CAGTTTCTGGGGCTAATGATATTATTTCTGGTAAATCATCACTCGATAGTCTAGGAATTAAAGCCGAGTCAGATACAGAA
TTTACAGTCACATTGGCACAACCAACACCATATTTTAAATTTCTCTTGTCAGAACCTGTTTATTACCCTCTTGACCAAAA
AGTTGTTGATAAATATGGCAAACAATATGGAACGTCCTCAGATAAAACCGTTTATAATGGACCATTCATGTTCAAATCAG
ATAAAGGTTGGACAGGAACCAATAAAACTTTTTCAATTTATGCCAATCCAAACTACTATGATAAGTCGGCAGTCAAATCT
AAACAAATTGATTTCCAGGTCATTTCAAATGCGAATACAGGAGCACAGCTTTATAAACAAGGGAAACTTGATTTTACACT
CCTTTCAACAACTGATTTGATTAATGCCAATAAAAAGACGAAAGGTTATACTGTCTTTAAACAAGCACGGACAGACTATA
TTGAATACAATCAATCCGGTAAAAATGCCTCAAGTCCAGACGCTCAAAAAGCACTAGCTAATCAAAATATTCGTGAAGCC
TTAAATTTGGCAACTAACAGAGCAGGAGTCGTCAAAACAGCCCTACCAGGTTCGACCGTTGCGACAAGCTTTACACCAGT
GGGAATGAGCAAGACCTCAACAGGAGAAGATTTTGCAACTTATGCAAAACAAGATTATCGCTATGACCCGACAAAAGCAA
AAGACCTCTGGGAAAAAGGATTAAAAGAGCTTGGCTTAACCAAGCTCACCCTTAGCCTTGAAGCTGCTGGTGATTTAGCA
CCGTCAGAAGCAACAGCCAACTTTTTACAAACAGCCTATCAACAAAATCTACCAGGCTTGACTGTCAATTTAAAACTTGT
TCCATTCAAACAAAGATTGAATGACGCTCAAAATGGAAATTTTGATATGGTTCTCTCAGGTTGGGGTGGAGATTACGCTG
AACCTTCAACATTCTTGCAACTCTTTACGACAGGCCAATCATATAATGACGGTAAATTTTCTAGTAAAACTTATGATGAT
GCCTTTAAAGCAGCAACAACAACGCCAGACGTTCTTGAACCAGCCAAAGTAGATGAACACTACAAAGCGGCAGAAACAGC
ACTCTATCAAGGGTCATACATCAATCCAGTTGATTTCCAAGCCAATCCAGCTTTAATGAACCCTAAAATTACAGGCTTAG
AATTTCATTCAACCGGTTTAGCTTATGACCTAAAATCGGCTTATGTTAAATAA

Upstream 100 bases:

>100_bases
ATTCTTGACAATGTATTTTTGAAAAAGTATAATAAGTCCACATTTAATTTTCAGAATAATATGAAAATTCAATAGTATCT
TTACGATTTGGAGAAAGCAG

Downstream 100 bases:

>100_bases
AAAATACAAAAAGATAAATTTTCTGAAAATTTATCTTTTTTATTGAATATTCTTTCTGCAAAAATAAAACACGAACCAGT
CAGAGCCCAACTAAATGAAA

Product: oligopeptide ABC transporter substrate binding protein

Products: NA

Alternate protein names: Stage 0 sporulation protein KA [H]

Number of amino acids: Translated: 550; Mature: 550

Protein sequence:

>550_residues
MKQGKIIGLTSLIALSGIILVACGTKTSEQKNIKFSIPTDVASLDTTILTDQYSYDVAGNVEEGLTRVDSKGNAALALAK
SIDVSKDGLTYTVTLRDNLKWSNGDRLTAKDFVYSWKRAVDPKTGSEYAYLMGAVSGANDIISGKSSLDSLGIKAESDTE
FTVTLAQPTPYFKFLLSEPVYYPLDQKVVDKYGKQYGTSSDKTVYNGPFMFKSDKGWTGTNKTFSIYANPNYYDKSAVKS
KQIDFQVISNANTGAQLYKQGKLDFTLLSTTDLINANKKTKGYTVFKQARTDYIEYNQSGKNASSPDAQKALANQNIREA
LNLATNRAGVVKTALPGSTVATSFTPVGMSKTSTGEDFATYAKQDYRYDPTKAKDLWEKGLKELGLTKLTLSLEAAGDLA
PSEATANFLQTAYQQNLPGLTVNLKLVPFKQRLNDAQNGNFDMVLSGWGGDYAEPSTFLQLFTTGQSYNDGKFSSKTYDD
AFKAATTTPDVLEPAKVDEHYKAAETALYQGSYINPVDFQANPALMNPKITGLEFHSTGLAYDLKSAYVK

Sequences:

>Translated_550_residues
MKQGKIIGLTSLIALSGIILVACGTKTSEQKNIKFSIPTDVASLDTTILTDQYSYDVAGNVEEGLTRVDSKGNAALALAK
SIDVSKDGLTYTVTLRDNLKWSNGDRLTAKDFVYSWKRAVDPKTGSEYAYLMGAVSGANDIISGKSSLDSLGIKAESDTE
FTVTLAQPTPYFKFLLSEPVYYPLDQKVVDKYGKQYGTSSDKTVYNGPFMFKSDKGWTGTNKTFSIYANPNYYDKSAVKS
KQIDFQVISNANTGAQLYKQGKLDFTLLSTTDLINANKKTKGYTVFKQARTDYIEYNQSGKNASSPDAQKALANQNIREA
LNLATNRAGVVKTALPGSTVATSFTPVGMSKTSTGEDFATYAKQDYRYDPTKAKDLWEKGLKELGLTKLTLSLEAAGDLA
PSEATANFLQTAYQQNLPGLTVNLKLVPFKQRLNDAQNGNFDMVLSGWGGDYAEPSTFLQLFTTGQSYNDGKFSSKTYDD
AFKAATTTPDVLEPAKVDEHYKAAETALYQGSYINPVDFQANPALMNPKITGLEFHSTGLAYDLKSAYVK
>Mature_550_residues
MKQGKIIGLTSLIALSGIILVACGTKTSEQKNIKFSIPTDVASLDTTILTDQYSYDVAGNVEEGLTRVDSKGNAALALAK
SIDVSKDGLTYTVTLRDNLKWSNGDRLTAKDFVYSWKRAVDPKTGSEYAYLMGAVSGANDIISGKSSLDSLGIKAESDTE
FTVTLAQPTPYFKFLLSEPVYYPLDQKVVDKYGKQYGTSSDKTVYNGPFMFKSDKGWTGTNKTFSIYANPNYYDKSAVKS
KQIDFQVISNANTGAQLYKQGKLDFTLLSTTDLINANKKTKGYTVFKQARTDYIEYNQSGKNASSPDAQKALANQNIREA
LNLATNRAGVVKTALPGSTVATSFTPVGMSKTSTGEDFATYAKQDYRYDPTKAKDLWEKGLKELGLTKLTLSLEAAGDLA
PSEATANFLQTAYQQNLPGLTVNLKLVPFKQRLNDAQNGNFDMVLSGWGGDYAEPSTFLQLFTTGQSYNDGKFSSKTYDD
AFKAATTTPDVLEPAKVDEHYKAAETALYQGSYINPVDFQANPALMNPKITGLEFHSTGLAYDLKSAYVK

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, It binds peptides up to five amino acids long with high affinity. Also required for sporulation and competence [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789397, Length=562, Percent_Identity=27.2241992882562, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1787495, Length=526, Percent_Identity=30.4182509505703, Blast_Score=175, Evalue=8e-45,
Organism=Escherichia coli, GI87081878, Length=535, Percent_Identity=29.1588785046729, Blast_Score=172, Evalue=6e-44,
Organism=Escherichia coli, GI1787052, Length=561, Percent_Identity=24.4206773618538, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI1789887, Length=459, Percent_Identity=25.4901960784314, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1789966, Length=486, Percent_Identity=25.1028806584362, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1787762, Length=515, Percent_Identity=23.8834951456311, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60023; Mature: 60023

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQGKIIGLTSLIALSGIILVACGTKTSEQKNIKFSIPTDVASLDTTILTDQYSYDVAGN
CCCCCEEHHHHHHHHCCEEEEEECCCCCCCCCEEEECCCCCHHCCEEEEECCCCCCCCCC
VEEGLTRVDSKGNAALALAKSIDVSKDGLTYTVTLRDNLKWSNGDRLTAKDFVYSWKRAV
HHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEEEECCCCCCCCCEEEHHHHHHHHHHHC
DPKTGSEYAYLMGAVSGANDIISGKSSLDSLGIKAESDTEFTVTLAQPTPYFKFLLSEPV
CCCCCCCEEEEEEECCCCCHHHCCCCCHHHCCCEECCCCEEEEEEECCCHHHHHHHCCCC
YYPLDQKVVDKYGKQYGTSSDKTVYNGPFMFKSDKGWTGTNKTFSIYANPNYYDKSAVKS
CCCCCHHHHHHHHHHHCCCCCCEEECCCEEEECCCCCCCCCCEEEEEECCCCCCHHHHHC
KQIDFQVISNANTGAQLYKQGKLDFTLLSTTDLINANKKTKGYTVFKQARTDYIEYNQSG
CCCEEEEECCCCCCHHHHHCCCCEEEEEECHHHHCCCCCCCCEEEEHHHCCHHEEECCCC
KNASSPDAQKALANQNIREALNLATNRAGVVKTALPGSTVATSFTPVGMSKTSTGEDFAT
CCCCCCHHHHHHHCCHHHHHHHHHCCCCCEEEECCCCCCCCCEECCCCCCCCCCCCHHHH
YAKQDYRYDPTKAKDLWEKGLKELGLTKLTLSLEAAGDLAPSEATANFLQTAYQQNLPGL
HHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCE
TVNLKLVPFKQRLNDAQNGNFDMVLSGWGGDYAEPSTFLQLFTTGQSYNDGKFSSKTYDD
EEEEEEEEHHHHCCCCCCCCEEEEEECCCCCCCCHHHEEEEEECCCCCCCCCCCCCCHHH
AFKAATTTPDVLEPAKVDEHYKAAETALYQGSYINPVDFQANPALMNPKITGLEFHSTGL
HHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCEEEECCCC
AYDLKSAYVK
EEEHHHHHCC
>Mature Secondary Structure
MKQGKIIGLTSLIALSGIILVACGTKTSEQKNIKFSIPTDVASLDTTILTDQYSYDVAGN
CCCCCEEHHHHHHHHCCEEEEEECCCCCCCCCEEEECCCCCHHCCEEEEECCCCCCCCCC
VEEGLTRVDSKGNAALALAKSIDVSKDGLTYTVTLRDNLKWSNGDRLTAKDFVYSWKRAV
HHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEEEECCCCCCCCCEEEHHHHHHHHHHHC
DPKTGSEYAYLMGAVSGANDIISGKSSLDSLGIKAESDTEFTVTLAQPTPYFKFLLSEPV
CCCCCCCEEEEEEECCCCCHHHCCCCCHHHCCCEECCCCEEEEEEECCCHHHHHHHCCCC
YYPLDQKVVDKYGKQYGTSSDKTVYNGPFMFKSDKGWTGTNKTFSIYANPNYYDKSAVKS
CCCCCHHHHHHHHHHHCCCCCCEEECCCEEEECCCCCCCCCCEEEEEECCCCCCHHHHHC
KQIDFQVISNANTGAQLYKQGKLDFTLLSTTDLINANKKTKGYTVFKQARTDYIEYNQSG
CCCEEEEECCCCCCHHHHHCCCCEEEEEECHHHHCCCCCCCCEEEEHHHCCHHEEECCCC
KNASSPDAQKALANQNIREALNLATNRAGVVKTALPGSTVATSFTPVGMSKTSTGEDFAT
CCCCCCHHHHHHHCCHHHHHHHHHCCCCCEEEECCCCCCCCCEECCCCCCCCCCCCHHHH
YAKQDYRYDPTKAKDLWEKGLKELGLTKLTLSLEAAGDLAPSEATANFLQTAYQQNLPGL
HHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCE
TVNLKLVPFKQRLNDAQNGNFDMVLSGWGGDYAEPSTFLQLFTTGQSYNDGKFSSKTYDD
EEEEEEEEHHHHCCCCCCCCEEEEEECCCCCCCCHHHEEEEEECCCCCCCCCCCCCCHHH
AFKAATTTPDVLEPAKVDEHYKAAETALYQGSYINPVDFQANPALMNPKITGLEFHSTGL
HHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCEEEECCCC
AYDLKSAYVK
EEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1901616; 1899858; 9384377 [H]