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The map label for this gene is murF

Identifier: 15672324

GI number: 15672324

Start: 341850

End: 343172

Strand: Direct

Name: murF

Synonym: L141766

Alternate gene names: 15672324

Gene position: 341850-343172 (Clockwise)

Preceding gene: 15672323

Following gene: 15672325

Centisome position: 14.45

GC content: 35.98

Gene sequence:

>1323_bases
ATGAAACTCACAATTCACGAAATCGCCCAAGTCGTTGGCGCAAAAAATGATTGGTCGCAACTGGCAGATTTGTCAGTCAA
TAAAATTGAATTTGACAGCCGTTTGATTGAAAAAGGGGATATCTTTTTGCCACTTAAGGGGGCACGAGATGGTCATGATT
TCATCGAAATCGCTTTTGATAATGGAGCAATAATCAGCTTTTCAGAAAAAGAAGTCGAACAAGCTCATCTCTTGGTTGAC
GATAATCTGCTTGCTTTCCAAAAATTAGCAAAATATTATCTTGAAAAGACAAAAGTTCCAGTCATTGCGGTGACAGGTTC
AAATGGAAAAACAACAACCAAAGACATGATTGCTGCTGTTTTGTCTAAGAAATTTAAAACATACAAAACACAAGGAAATC
ACAATAATGAAATCGGCTTACCCTACACCATTTTACATATGCCGGACGATACTGAAAAATTAGTTTTGGAAATGGGAATG
GATCACCCTGGTGATATTGATTTTTTATCAGAACTTGCTAAGCCTGAACTTGCTGTCATTACCTTAATTGGTGAAGCACA
TCTTGAACATATGGGAAGTCGCGAAAATATTGCTAAAGGGAAAATGGGGATTACGGCTGGTTTGCATGGTGAGCTTATTG
CCCCTGCTGACCCAATTATTAATGCTTTTATCCCAGACAATCAAAAAATTATTCGCTTTGGTTTGCCCGGTGAAGATTTA
TTTATTACAAAATTAGTCGAACACAAAGAAAAATTAACTTTTGAAACTAATTTTTTAGATGAATCAATTACTATTCCAGT
TCCTGGTAAATATAACGCGACGAATGCTATGTTAGCTGCTTTTGTTGGCTTACATTATGGACTTTCAGAAGCCGAAATTA
AAAAAGCTTTGAAAGAAGTAGAGCTGACCAGAAATCGGACAGAGTGGAAAAAAGCAAAAAATGGTGCAGACCTTTTGAGT
GATGTTTATAATGCCAATCCGACAGCAATGCGTTTGATTTTGGAAACTTTCCAAGCCATTCCTAAAAATGAGAATGGTCG
AAAGATTGCAGTTCTTGCTGATATGTTAGAACTTGGACCCAGTGCAGCCCAACTCCATAAAGATATATTGAAATCTATTG
ATTTCAATAAAATTGATAAAGTCTATCTTTATGGTGAAATGATGAAAAACTTGGCAGAAATTTCGACTGACAAAGCGGTC
AGCTATTTTACAGATTTAGATTTACTGACAGAAAGTCTGTCAGCAGATTTGAAACCAACTGACCAAGTATTATTCAAAGG
TTCAAATAGCATGAAACTTTCAGAGGTTGTTGAAAAATTATAA

Upstream 100 bases:

>100_bases
AAACACGTGAAAATCATCTTTTATAAAAAATACAAATCTGTCAGTAAATCGATTGCTGACAGATTTTTTTGTCAGTAAAA
AGTGTTATAATGGAGTCACT

Downstream 100 bases:

>100_bases
TGATAAAAAATACTGACAGAAAGTTAGTATTTTTTTATTTTCATTAAATTATCAGAAAAATACAACAATATTCTTGACAA
TGTATTTTTGAAAAAGTATA

Product: D-Ala-D-Ala adding enzyme

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MKLTIHEIAQVVGAKNDWSQLADLSVNKIEFDSRLIEKGDIFLPLKGARDGHDFIEIAFDNGAIISFSEKEVEQAHLLVD
DNLLAFQKLAKYYLEKTKVPVIAVTGSNGKTTTKDMIAAVLSKKFKTYKTQGNHNNEIGLPYTILHMPDDTEKLVLEMGM
DHPGDIDFLSELAKPELAVITLIGEAHLEHMGSRENIAKGKMGITAGLHGELIAPADPIINAFIPDNQKIIRFGLPGEDL
FITKLVEHKEKLTFETNFLDESITIPVPGKYNATNAMLAAFVGLHYGLSEAEIKKALKEVELTRNRTEWKKAKNGADLLS
DVYNANPTAMRLILETFQAIPKNENGRKIAVLADMLELGPSAAQLHKDILKSIDFNKIDKVYLYGEMMKNLAEISTDKAV
SYFTDLDLLTESLSADLKPTDQVLFKGSNSMKLSEVVEKL

Sequences:

>Translated_440_residues
MKLTIHEIAQVVGAKNDWSQLADLSVNKIEFDSRLIEKGDIFLPLKGARDGHDFIEIAFDNGAIISFSEKEVEQAHLLVD
DNLLAFQKLAKYYLEKTKVPVIAVTGSNGKTTTKDMIAAVLSKKFKTYKTQGNHNNEIGLPYTILHMPDDTEKLVLEMGM
DHPGDIDFLSELAKPELAVITLIGEAHLEHMGSRENIAKGKMGITAGLHGELIAPADPIINAFIPDNQKIIRFGLPGEDL
FITKLVEHKEKLTFETNFLDESITIPVPGKYNATNAMLAAFVGLHYGLSEAEIKKALKEVELTRNRTEWKKAKNGADLLS
DVYNANPTAMRLILETFQAIPKNENGRKIAVLADMLELGPSAAQLHKDILKSIDFNKIDKVYLYGEMMKNLAEISTDKAV
SYFTDLDLLTESLSADLKPTDQVLFKGSNSMKLSEVVEKL
>Mature_440_residues
MKLTIHEIAQVVGAKNDWSQLADLSVNKIEFDSRLIEKGDIFLPLKGARDGHDFIEIAFDNGAIISFSEKEVEQAHLLVD
DNLLAFQKLAKYYLEKTKVPVIAVTGSNGKTTTKDMIAAVLSKKFKTYKTQGNHNNEIGLPYTILHMPDDTEKLVLEMGM
DHPGDIDFLSELAKPELAVITLIGEAHLEHMGSRENIAKGKMGITAGLHGELIAPADPIINAFIPDNQKIIRFGLPGEDL
FITKLVEHKEKLTFETNFLDESITIPVPGKYNATNAMLAAFVGLHYGLSEAEIKKALKEVELTRNRTEWKKAKNGADLLS
DVYNANPTAMRLILETFQAIPKNENGRKIAVLADMLELGPSAAQLHKDILKSIDFNKIDKVYLYGEMMKNLAEISTDKAV
SYFTDLDLLTESLSADLKPTDQVLFKGSNSMKLSEVVEKL

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=440, Percent_Identity=31.3636363636364, Blast_Score=172, Evalue=5e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 48824; Mature: 48824

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTIHEIAQVVGAKNDWSQLADLSVNKIEFDSRLIEKGDIFLPLKGARDGHDFIEIAFD
CEEEHHHHHHHHCCCCCHHHHHCCCCCEEEHHHHHHCCCCEEEEECCCCCCCCEEEEEEC
NGAIISFSEKEVEQAHLLVDDNLLAFQKLAKYYLEKTKVPVIAVTGSNGKTTTKDMIAAV
CCEEEEECHHHHHHHHEEECCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHH
LSKKFKTYKTQGNHNNEIGLPYTILHMPDDTEKLVLEMGMDHPGDIDFLSELAKPELAVI
HHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHCCCCEEEE
TLIGEAHLEHMGSRENIAKGKMGITAGLHGELIAPADPIINAFIPDNQKIIRFGLPGEDL
EEEHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCHHHEEECCCCCEEEEECCCCCCH
FITKLVEHKEKLTFETNFLDESITIPVPGKYNATNAMLAAFVGLHYGLSEAEIKKALKEV
HHHHHHHHHHHCEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ELTRNRTEWKKAKNGADLLSDVYNANPTAMRLILETFQAIPKNENGRKIAVLADMLELGP
HHHCCHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHCCC
SAAQLHKDILKSIDFNKIDKVYLYGEMMKNLAEISTDKAVSYFTDLDLLTESLSADLKPT
CHHHHHHHHHHHCCCCCCHHHEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCC
DQVLFKGSNSMKLSEVVEKL
HHHEEECCCCCCHHHHHHHC
>Mature Secondary Structure
MKLTIHEIAQVVGAKNDWSQLADLSVNKIEFDSRLIEKGDIFLPLKGARDGHDFIEIAFD
CEEEHHHHHHHHCCCCCHHHHHCCCCCEEEHHHHHHCCCCEEEEECCCCCCCCEEEEEEC
NGAIISFSEKEVEQAHLLVDDNLLAFQKLAKYYLEKTKVPVIAVTGSNGKTTTKDMIAAV
CCEEEEECHHHHHHHHEEECCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHH
LSKKFKTYKTQGNHNNEIGLPYTILHMPDDTEKLVLEMGMDHPGDIDFLSELAKPELAVI
HHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHCCCCEEEE
TLIGEAHLEHMGSRENIAKGKMGITAGLHGELIAPADPIINAFIPDNQKIIRFGLPGEDL
EEEHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCHHHEEECCCCCEEEEECCCCCCH
FITKLVEHKEKLTFETNFLDESITIPVPGKYNATNAMLAAFVGLHYGLSEAEIKKALKEV
HHHHHHHHHHHCEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ELTRNRTEWKKAKNGADLLSDVYNANPTAMRLILETFQAIPKNENGRKIAVLADMLELGP
HHHCCHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHCCC
SAAQLHKDILKSIDFNKIDKVYLYGEMMKNLAEISTDKAVSYFTDLDLLTESLSADLKPT
CHHHHHHHHHHHCCCCCCHHHEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCC
DQVLFKGSNSMKLSEVVEKL
HHHEEECCCCCCHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]