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The map label for this gene is ddl

Identifier: 15672323

GI number: 15672323

Start: 340726

End: 341775

Strand: Direct

Name: ddl

Synonym: L140690

Alternate gene names: 15672323

Gene position: 340726-341775 (Clockwise)

Preceding gene: 15672322

Following gene: 15672324

Centisome position: 14.4

GC content: 35.9

Gene sequence:

>1050_bases
ATGTCAAAAGAAACATTAATTTTATTGTATGGTGGTCGTTCTGCTGAGCGAGAAGTATCAGTTTTATCAGCAGAATCGGT
GATGAGAGCTGTTAATTATTCTCGTTTTGTTGTAAAAACTTACTTTATCACTAAGGGTGGTGAATTCATAAAAACGCAAG
AATTTACTGACAAACCTGCTGAAGAAGAAAAACTACTGACAAATGATTTGGCAGAATCTTATCCAAAGATTTCACCAGCA
GCAATTTACGAAAAAGCTGCCGTTGTATTCCCAGTTTTACATGGACCAATGGGTGAAGATGGCTCGATTCAAGGATTTTT
AGAAATTTTACGTTTGGCATATGTTGGTCCAAATATTTTATCTGCGTCAAGCACAATGGATAAATTATTGGCAAAACACG
TTTTTGAAGCGGTTGGTGTACCCCAAGTTCCTTATGTTGCGGCTTTTGCTGATGAAAATCAAGGAGAAATTGCTCAAGAA
GTAGTCGAAAAATTAGAATTTCCAGTTTTTGTTAAACCTGCTAATATGGGTTCTAGTGTTGGAATTTCAAAAGTTGATGA
TTTAGCAGACCTTCAACCTGCGCTAAGCGAAGCCTATAAATATGATAACCGTGTTGTCATTGAACAAGGTGTGGATGCGC
GTGAAATCGAATGTGCTGTTTTGGGAAATAATAGTGACGTTTCTGCAACGCTTCCAGGTGAAGTTGTGAAAGATGTTGAG
TTTTATGATTACAACTCAAAATATATTGATAATAAAATTCAAATGGACATTCCAGCAAAAGTTCCTGCTGATTTAGCTCA
AAAAATGCAAGAATATGCTATAAAAGCGTACAAGGCAGTCAATGGGACGGGCTTGTCACGTTGCGATTTCTTTGTGACTA
AGGATGGAAATGTTTATCTCAACGAAATTAATGCCATTCCAGGATTCACACAATGGTCAATGTATCCATTGCTTTGGGAA
AATATGGGCTTATCTTATTCTGATTTAATTGAAAAGTTAGTTGATTTAGCTAAAGAAACTTTTGAAACACGTGAAAATCA
TCTTTTATAA

Upstream 100 bases:

>100_bases
ATAAAAAAGACAATTACTGACAGAATTGTATTGTAAAAATATCGCATGCTGTCAGTAAAAAGAAGTGTCTGACAAAGCTG
TCAGTAAAAAAGGAAGAAAT

Downstream 100 bases:

>100_bases
AAAATACAAATCTGTCAGTAAATCGATTGCTGACAGATTTTTTTGTCAGTAAAAAGTGTTATAATGGAGTCACTATGAAA
CTCACAATTCACGAAATCGC

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MSKETLILLYGGRSAEREVSVLSAESVMRAVNYSRFVVKTYFITKGGEFIKTQEFTDKPAEEEKLLTNDLAESYPKISPA
AIYEKAAVVFPVLHGPMGEDGSIQGFLEILRLAYVGPNILSASSTMDKLLAKHVFEAVGVPQVPYVAAFADENQGEIAQE
VVEKLEFPVFVKPANMGSSVGISKVDDLADLQPALSEAYKYDNRVVIEQGVDAREIECAVLGNNSDVSATLPGEVVKDVE
FYDYNSKYIDNKIQMDIPAKVPADLAQKMQEYAIKAYKAVNGTGLSRCDFFVTKDGNVYLNEINAIPGFTQWSMYPLLWE
NMGLSYSDLIEKLVDLAKETFETRENHLL

Sequences:

>Translated_349_residues
MSKETLILLYGGRSAEREVSVLSAESVMRAVNYSRFVVKTYFITKGGEFIKTQEFTDKPAEEEKLLTNDLAESYPKISPA
AIYEKAAVVFPVLHGPMGEDGSIQGFLEILRLAYVGPNILSASSTMDKLLAKHVFEAVGVPQVPYVAAFADENQGEIAQE
VVEKLEFPVFVKPANMGSSVGISKVDDLADLQPALSEAYKYDNRVVIEQGVDAREIECAVLGNNSDVSATLPGEVVKDVE
FYDYNSKYIDNKIQMDIPAKVPADLAQKMQEYAIKAYKAVNGTGLSRCDFFVTKDGNVYLNEINAIPGFTQWSMYPLLWE
NMGLSYSDLIEKLVDLAKETFETRENHLL
>Mature_348_residues
SKETLILLYGGRSAEREVSVLSAESVMRAVNYSRFVVKTYFITKGGEFIKTQEFTDKPAEEEKLLTNDLAESYPKISPAA
IYEKAAVVFPVLHGPMGEDGSIQGFLEILRLAYVGPNILSASSTMDKLLAKHVFEAVGVPQVPYVAAFADENQGEIAQEV
VEKLEFPVFVKPANMGSSVGISKVDDLADLQPALSEAYKYDNRVVIEQGVDAREIECAVLGNNSDVSATLPGEVVKDVEF
YDYNSKYIDNKIQMDIPAKVPADLAQKMQEYAIKAYKAVNGTGLSRCDFFVTKDGNVYLNEINAIPGFTQWSMYPLLWEN
MGLSYSDLIEKLVDLAKETFETRENHLL

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786579, Length=363, Percent_Identity=36.9146005509642, Blast_Score=244, Evalue=7e-66,
Organism=Escherichia coli, GI1786280, Length=343, Percent_Identity=31.4868804664723, Blast_Score=160, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_LACLA (Q9CIL5)

Other databases:

- EMBL:   AE005176
- PIR:   E86667
- RefSeq:   NP_266497.1
- ProteinModelPortal:   Q9CIL5
- SMR:   Q9CIL5
- GeneID:   1113952
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L140690
- NMPDR:   fig|272623.1.peg.349
- HOGENOM:   HBG427092
- OMA:   FTDISMY
- ProtClustDB:   PRK01966
- BioCyc:   LLAC272623:L140690-MONOMER
- BRENDA:   6.3.2.4
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 38693; Mature: 38562

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKETLILLYGGRSAEREVSVLSAESVMRAVNYSRFVVKTYFITKGGEFIKTQEFTDKPA
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHEEEEEEEEECCCCEEECCCCCCCCC
EEEKLLTNDLAESYPKISPAAIYEKAAVVFPVLHGPMGEDGSIQGFLEILRLAYVGPNIL
HHHHHHHHHHHHHCCCCCHHHHHHHHHHEEHHHCCCCCCCCCHHHHHHHHHHHHCCCCHH
SASSTMDKLLAKHVFEAVGVPQVPYVAAFADENQGEIAQEVVEKLEFPVFVKPANMGSSV
HHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCC
GISKVDDLADLQPALSEAYKYDNRVVIEQGVDAREIECAVLGNNSDVSATLPGEVVKDVE
CCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCCCCCEEECCHHHHHCCC
FYDYNSKYIDNKIQMDIPAKVPADLAQKMQEYAIKAYKAVNGTGLSRCDFFVTKDGNVYL
EEECCCHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEE
NEINAIPGFTQWSMYPLLWENMGLSYSDLIEKLVDLAKETFETRENHLL
EEECCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKETLILLYGGRSAEREVSVLSAESVMRAVNYSRFVVKTYFITKGGEFIKTQEFTDKPA
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHEEEEEEEEECCCCEEECCCCCCCCC
EEEKLLTNDLAESYPKISPAAIYEKAAVVFPVLHGPMGEDGSIQGFLEILRLAYVGPNIL
HHHHHHHHHHHHHCCCCCHHHHHHHHHHEEHHHCCCCCCCCCHHHHHHHHHHHHCCCCHH
SASSTMDKLLAKHVFEAVGVPQVPYVAAFADENQGEIAQEVVEKLEFPVFVKPANMGSSV
HHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCC
GISKVDDLADLQPALSEAYKYDNRVVIEQGVDAREIECAVLGNNSDVSATLPGEVVKDVE
CCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCCCCCEEECCHHHHHCCC
FYDYNSKYIDNKIQMDIPAKVPADLAQKMQEYAIKAYKAVNGTGLSRCDFFVTKDGNVYL
EEECCCHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEE
NEINAIPGFTQWSMYPLLWENMGLSYSDLIEKLVDLAKETFETRENHLL
EEECCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471