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The map label for this gene is ycjM

Identifier: 15672285

GI number: 15672285

Start: 299840

End: 301399

Strand: Direct

Name: ycjM

Synonym: L99798

Alternate gene names: 15672285

Gene position: 299840-301399 (Clockwise)

Preceding gene: 15672284

Following gene: 15672286

Centisome position: 12.68

GC content: 36.47

Gene sequence:

>1560_bases
ATGAAAATAAGAATTCTAGAAACGAGTGATTTACATGGTTTTGTTCTCCCAACAAACTTCACCGAAAGAGAAATGAATTT
GCCTTTTTCAATGGCCAAAGCCAAATCTAAAATAGATGAACTGACAGCAGTGGCTCAAGAGCAAGGTGAAATTATTGTAA
AAATTGAAAATGGTGATTTATTGCAAGGTTCCCCCTTGGCTTATTATCTAGCTAAAAAATCAAGTCGCGGTATTAAAGAC
TTGGTCAATGTCACTAATTCTTTTGGTTATGATGTGGGATTGCTTGGTAATCATGAGTTTAATTATGGGATTGATTATTT
AAAAAGTTATGTTAATCAGGTAAATTATCCTATTTTGGCTGCGAATGTATTGAATAATGAAGGTCAACCTGCTTTTGGCC
CTGCTTATAAAATCATTGAAGCGCAGGGGGTGAAAATTGGAATTGTTGGTTTTTTGACACAATATATTCCACATTGGGAA
AAACCGTCAATTATCAAAGATTTGACTTTCCAATCTATTCTGACAACTGCAAAAGATATTCTACCCGATTTGCGCCAATC
AGTTGACCTTTTGATTGTTGCATATCATGGAGGCTTTGAGCGAGATTTACAGACAGGTCTTCCGACAGAAAGTTTGACAG
GGGAAAATGAGGCTTATCAGTTAGTTGAAGAGTATAAAGATTACATTGATGCCTTAGTGACTGGACACCAGCATCGTGAA
ATTGCTGACCATCTTTTTGGAGTTCCAGTTATTCAACCAGGCTATCGTGGTGCTTTTGTTGGCGAAATAGAACTTGAATT
AGATAATCATAAAAAAGTTGTGGCTTCAAACGCTAGACTTCATAAAACGGAGAATTGCAAAGTCTCATCGGAGGTCAATC
AGCTGATATCCGTTGTGAGTGAAGAAGCAGAGGATTGGTTAGACCAGCCAATGGGAGAAGTTCATGGAGAAATGATTATT
CGTGATCCAATGACTGCTCGATTGATTGAACATCCTTATATTGAATTGATTAATAAAATACAAATGGAAGCGACAGGGAC
CGAAATTTCTGGGACTGCACTTTTTAATAATGAAGCGAAGGGTTTTGCAAAAGAAATTTCGATGAGATCGATTCTGACAA
ATTATATTTATCCCAACACTTTAGCTGTTTTAAGAGTGACAGGTGCAGACTTAAAGGCTGCCTTAGAGCGGACAGCGGAA
CATCTTGAACTTGACAAAAGTGGCGAAATTGTTTTTAGTCCACGCTTTATTGAACCAAAACCTGAATATTATAATTATGA
TATGTATGAAGGAGTTGAATATGCTATCAATTTGCAAAAAGAAATTGGTCAGCGAATTGTACGTTTAAATTTCAAGGGGC
ATCAGGTAAAAGATGACGAAATTTTAGAAATTGTCGTCAATCAATATCGCGCAGTTGGGGGTGGAAATTATCCAATGTTT
TCTGCGGACAAGATTGTTAGAGAAATTACTGTTGATATGACCGAATTGATTGCTGATTATTTGAAGAAACATCCGATAAT
TGAGGCAAGTGTGAATCATAACTTCACAGTTCTAAAATAA

Upstream 100 bases:

>100_bases
CAGCCGTTGGTGCAATTTTGTTGGGACTTTTAGTTTTAATTATCCTTGTTGAACTCATTTCATCAAGAATTCGGAAAAAA
TTAGCAAAGGGATAAATAAT

Downstream 100 bases:

>100_bases
ATTTACTGACAGAAATTTTAGCAGAATAATAGGTGCTAAAAAAATCTGTCAGTAATTTTTTTATTAAGTTATACTTGAGA
ATGAGCTAAGTTTTTCTAAA

Product: phosphatase

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MKIRILETSDLHGFVLPTNFTEREMNLPFSMAKAKSKIDELTAVAQEQGEIIVKIENGDLLQGSPLAYYLAKKSSRGIKD
LVNVTNSFGYDVGLLGNHEFNYGIDYLKSYVNQVNYPILAANVLNNEGQPAFGPAYKIIEAQGVKIGIVGFLTQYIPHWE
KPSIIKDLTFQSILTTAKDILPDLRQSVDLLIVAYHGGFERDLQTGLPTESLTGENEAYQLVEEYKDYIDALVTGHQHRE
IADHLFGVPVIQPGYRGAFVGEIELELDNHKKVVASNARLHKTENCKVSSEVNQLISVVSEEAEDWLDQPMGEVHGEMII
RDPMTARLIEHPYIELINKIQMEATGTEISGTALFNNEAKGFAKEISMRSILTNYIYPNTLAVLRVTGADLKAALERTAE
HLELDKSGEIVFSPRFIEPKPEYYNYDMYEGVEYAINLQKEIGQRIVRLNFKGHQVKDDEILEIVVNQYRAVGGGNYPMF
SADKIVREITVDMTELIADYLKKHPIIEASVNHNFTVLK

Sequences:

>Translated_519_residues
MKIRILETSDLHGFVLPTNFTEREMNLPFSMAKAKSKIDELTAVAQEQGEIIVKIENGDLLQGSPLAYYLAKKSSRGIKD
LVNVTNSFGYDVGLLGNHEFNYGIDYLKSYVNQVNYPILAANVLNNEGQPAFGPAYKIIEAQGVKIGIVGFLTQYIPHWE
KPSIIKDLTFQSILTTAKDILPDLRQSVDLLIVAYHGGFERDLQTGLPTESLTGENEAYQLVEEYKDYIDALVTGHQHRE
IADHLFGVPVIQPGYRGAFVGEIELELDNHKKVVASNARLHKTENCKVSSEVNQLISVVSEEAEDWLDQPMGEVHGEMII
RDPMTARLIEHPYIELINKIQMEATGTEISGTALFNNEAKGFAKEISMRSILTNYIYPNTLAVLRVTGADLKAALERTAE
HLELDKSGEIVFSPRFIEPKPEYYNYDMYEGVEYAINLQKEIGQRIVRLNFKGHQVKDDEILEIVVNQYRAVGGGNYPMF
SADKIVREITVDMTELIADYLKKHPIIEASVNHNFTVLK
>Mature_519_residues
MKIRILETSDLHGFVLPTNFTEREMNLPFSMAKAKSKIDELTAVAQEQGEIIVKIENGDLLQGSPLAYYLAKKSSRGIKD
LVNVTNSFGYDVGLLGNHEFNYGIDYLKSYVNQVNYPILAANVLNNEGQPAFGPAYKIIEAQGVKIGIVGFLTQYIPHWE
KPSIIKDLTFQSILTTAKDILPDLRQSVDLLIVAYHGGFERDLQTGLPTESLTGENEAYQLVEEYKDYIDALVTGHQHRE
IADHLFGVPVIQPGYRGAFVGEIELELDNHKKVVASNARLHKTENCKVSSEVNQLISVVSEEAEDWLDQPMGEVHGEMII
RDPMTARLIEHPYIELINKIQMEATGTEISGTALFNNEAKGFAKEISMRSILTNYIYPNTLAVLRVTGADLKAALERTAE
HLELDKSGEIVFSPRFIEPKPEYYNYDMYEGVEYAINLQKEIGQRIVRLNFKGHQVKDDEILEIVVNQYRAVGGGNYPMF
SADKIVREITVDMTELIADYLKKHPIIEASVNHNFTVLK

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Escherichia coli, GI1790658, Length=549, Percent_Identity=26.0473588342441, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1786687, Length=552, Percent_Identity=21.7391304347826, Blast_Score=89, Evalue=1e-18,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 58413; Mature: 58413

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00785 5_NUCLEOTIDASE_1 ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRILETSDLHGFVLPTNFTEREMNLPFSMAKAKSKIDELTAVAQEQGEIIVKIENGDL
CEEEEEECCCCCEEEECCCCCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCE
LQGSPLAYYLAKKSSRGIKDLVNVTNSFGYDVGLLGNHEFNYGIDYLKSYVNQVNYPILA
ECCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCEEE
ANVLNNEGQPAFGPAYKIIEAQGVKIGIVGFLTQYIPHWEKPSIIKDLTFQSILTTAKDI
EEECCCCCCCCCCCHHHEEECCCCEEHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
LPDLRQSVDLLIVAYHGGFERDLQTGLPTESLTGENEAYQLVEEYKDYIDALVTGHQHRE
HHHHHCCCEEEEEEECCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHH
IADHLFGVPVIQPGYRGAFVGEIELELDNHKKVVASNARLHKTENCKVSSEVNQLISVVS
HHHHHCCCCCCCCCCCCEEEEEEEEEECCCCEEEECCCCEECCCCCCHHHHHHHHHHHHH
EEAEDWLDQPMGEVHGEMIIRDPMTARLIEHPYIELINKIQMEATGTEISGTALFNNEAK
HHHHHHHHCCHHHHCCCEEEECCHHHHHHCCHHHHHHHHHHHEECCCEECCEEEECCCCH
GFAKEISMRSILTNYIYPNTLAVLRVTGADLKAALERTAEHLELDKSGEIVFSPRFIEPK
HHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCC
PEYYNYDMYEGVEYAINLQKEIGQRIVRLNFKGHQVKDDEILEIVVNQYRAVGGGNYPMF
CCCCCCCHHCCHHHEEEHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
SADKIVREITVDMTELIADYLKKHPIIEASVNHNFTVLK
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEC
>Mature Secondary Structure
MKIRILETSDLHGFVLPTNFTEREMNLPFSMAKAKSKIDELTAVAQEQGEIIVKIENGDL
CEEEEEECCCCCEEEECCCCCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCE
LQGSPLAYYLAKKSSRGIKDLVNVTNSFGYDVGLLGNHEFNYGIDYLKSYVNQVNYPILA
ECCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCEEE
ANVLNNEGQPAFGPAYKIIEAQGVKIGIVGFLTQYIPHWEKPSIIKDLTFQSILTTAKDI
EEECCCCCCCCCCCHHHEEECCCCEEHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
LPDLRQSVDLLIVAYHGGFERDLQTGLPTESLTGENEAYQLVEEYKDYIDALVTGHQHRE
HHHHHCCCEEEEEEECCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHH
IADHLFGVPVIQPGYRGAFVGEIELELDNHKKVVASNARLHKTENCKVSSEVNQLISVVS
HHHHHCCCCCCCCCCCCEEEEEEEEEECCCCEEEECCCCEECCCCCCHHHHHHHHHHHHH
EEAEDWLDQPMGEVHGEMIIRDPMTARLIEHPYIELINKIQMEATGTEISGTALFNNEAK
HHHHHHHHCCHHHHCCCEEEECCHHHHHHCCHHHHHHHHHHHEECCCEECCEEEECCCCH
GFAKEISMRSILTNYIYPNTLAVLRVTGADLKAALERTAEHLELDKSGEIVFSPRFIEPK
HHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCC
PEYYNYDMYEGVEYAINLQKEIGQRIVRLNFKGHQVKDDEILEIVVNQYRAVGGGNYPMF
CCCCCCCHHCCHHHEEEHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
SADKIVREITVDMTELIADYLKKHPIIEASVNHNFTVLK
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]