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The map label for this gene is rmlB

Identifier: 15672180

GI number: 15672180

Start: 199239

End: 200288

Strand: Direct

Name: rmlB

Synonym: L199221

Alternate gene names: 15672180

Gene position: 199239-200288 (Clockwise)

Preceding gene: 15672179

Following gene: 15672181

Centisome position: 8.42

GC content: 37.52

Gene sequence:

>1050_bases
ATGACTGAATTTAAAAATATCGTTGTGACAGGTGGAGCAGGATTTATCGGCTCAAACTTTGTACACTATGTTTATAACAA
TCATCCAGACGTGCATATCACAGTTTTGGATAAATTAACTTATGCAGGAAATGTTAATAACATTAACATGTTATTTGACA
GTGGACGTGTCGAACTTGTTGTTGGCGACATTGCAGACCCTGAAATTGTTGATCAAGTGGCTTCTAAAGCAGACGCAATT
GTTCACTATGCAGCTGAAAGCCATAATGATAATTCTTTGAAATCTCAAGATGAATTTATCCAAACAAACTTTATCGGAAC
TTATACTTTGATTCAAGCAGCTCGTAAATATGATTTACGCTTCCACCATGTTTCTACTGATGAAGTTTATGGTGATTTGC
CTTATCGCGAAGATTTGCCAGGTCATGGCGAAGGTGAAGGTGAAAAATTTACTGACAAAACACCTTACAATCCTTCAAGC
CCTTACTCTTCAACTAAAGCGGCTTCTGATTTGATTGTTCGTGCTTGGGTCCGTTCATTTGGACTTAAAGCAACAATTTC
AAACTGTTCAAATAACTACGGACCTTTCCAACACATCGAAAAATTTATTCCTCGTCAAATTACTAATATTCTTTCAGGAA
TCAAACCAAAACTTTACGGTGATGGTAAAAACGTTCGTGACTGGATTCATACAGAAGACCATTCATCAGGTGTTTGGACA
ATTTTGACTAAAGGTCGAATGGGCGAAACTTATCTTATCGGTGCAGACGGTGAAAAAAATAACAAAGAAGTTCTTGAAGA
TATTTTGACTCGTATGGGCAAAGATAAAGACGATTATGACCGTGTAACTGACCGTGCTGGCCATGACCTTCGTTACGCGA
TTGATAATAGTAAATTACGTACAGAACTTGGTTGGGCTCCAAAACATACTGACTTTGAATCTGGTTTGCAAGCGACAATC
GACTGGTATCGTGAAAACGAAAACTGGTGGAAAGCTGAAAAAGAAGCTGTTGAAGCAAAATATGCTGAAACACAAAAAGT
TTTGGATTAA

Upstream 100 bases:

>100_bases
GAAAATATTTAAAAGCTGTGCCAAAAGCAAAATTTGTCAGAGAGTATCGAGTGGATTAATTTTCAAGATTTACTAATAAA
TAATCAAAAAAGGAAAAAAC

Downstream 100 bases:

>100_bases
TAAAATAAATTCCAATTATATTATCTAAAAGTTACTGACAGTAATAATGAAAAAATCTGTCAGTAAAAAATTAAAGATTG
GAGAACAAATGATTTTAATA

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MTEFKNIVVTGGAGFIGSNFVHYVYNNHPDVHITVLDKLTYAGNVNNINMLFDSGRVELVVGDIADPEIVDQVASKADAI
VHYAAESHNDNSLKSQDEFIQTNFIGTYTLIQAARKYDLRFHHVSTDEVYGDLPYREDLPGHGEGEGEKFTDKTPYNPSS
PYSSTKAASDLIVRAWVRSFGLKATISNCSNNYGPFQHIEKFIPRQITNILSGIKPKLYGDGKNVRDWIHTEDHSSGVWT
ILTKGRMGETYLIGADGEKNNKEVLEDILTRMGKDKDDYDRVTDRAGHDLRYAIDNSKLRTELGWAPKHTDFESGLQATI
DWYRENENWWKAEKEAVEAKYAETQKVLD

Sequences:

>Translated_349_residues
MTEFKNIVVTGGAGFIGSNFVHYVYNNHPDVHITVLDKLTYAGNVNNINMLFDSGRVELVVGDIADPEIVDQVASKADAI
VHYAAESHNDNSLKSQDEFIQTNFIGTYTLIQAARKYDLRFHHVSTDEVYGDLPYREDLPGHGEGEGEKFTDKTPYNPSS
PYSSTKAASDLIVRAWVRSFGLKATISNCSNNYGPFQHIEKFIPRQITNILSGIKPKLYGDGKNVRDWIHTEDHSSGVWT
ILTKGRMGETYLIGADGEKNNKEVLEDILTRMGKDKDDYDRVTDRAGHDLRYAIDNSKLRTELGWAPKHTDFESGLQATI
DWYRENENWWKAEKEAVEAKYAETQKVLD
>Mature_348_residues
TEFKNIVVTGGAGFIGSNFVHYVYNNHPDVHITVLDKLTYAGNVNNINMLFDSGRVELVVGDIADPEIVDQVASKADAIV
HYAAESHNDNSLKSQDEFIQTNFIGTYTLIQAARKYDLRFHHVSTDEVYGDLPYREDLPGHGEGEGEKFTDKTPYNPSSP
YSSTKAASDLIVRAWVRSFGLKATISNCSNNYGPFQHIEKFIPRQITNILSGIKPKLYGDGKNVRDWIHTEDHSSGVWTI
LTKGRMGETYLIGADGEKNNKEVLEDILTRMGKDKDDYDRVTDRAGHDLRYAIDNSKLRTELGWAPKHTDFESGLQATID
WYRENENWWKAEKEAVEAKYAETQKVLD

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=343, Percent_Identity=32.6530612244898, Blast_Score=191, Evalue=7e-49,
Organism=Homo sapiens, GI42516563, Length=350, Percent_Identity=23.1428571428571, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI48994969, Length=351, Percent_Identity=45.5840455840456, Blast_Score=309, Evalue=2e-85,
Organism=Escherichia coli, GI1788353, Length=342, Percent_Identity=45.0292397660819, Blast_Score=291, Evalue=3e-80,
Organism=Escherichia coli, GI1788366, Length=285, Percent_Identity=23.859649122807, Blast_Score=72, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17568069, Length=337, Percent_Identity=32.0474777448071, Blast_Score=168, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI115532424, Length=327, Percent_Identity=33.0275229357798, Blast_Score=167, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI17539532, Length=366, Percent_Identity=22.9508196721311, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21356223, Length=333, Percent_Identity=25.2252252252252, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24158427, Length=362, Percent_Identity=23.7569060773481, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 39461; Mature: 39330

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEFKNIVVTGGAGFIGSNFVHYVYNNHPDVHITVLDKLTYAGNVNNINMLFDSGRVELV
CCCCCEEEEECCCCCCCCCEEEEEECCCCCEEEEEEECCHHCCCCCCEEEEEECCCEEEE
VGDIADPEIVDQVASKADAIVHYAAESHNDNSLKSQDEFIQTNFIGTYTLIQAARKYDLR
ECCCCCHHHHHHHHHHHHHEEEEHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCEE
FHHVSTDEVYGDLPYREDLPGHGEGEGEKFTDKTPYNPSSPYSSTKAASDLIVRAWVRSF
EEECCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
GLKATISNCSNNYGPFQHIEKFIPRQITNILSGIKPKLYGDGKNVRDWIHTEDHSSGVWT
CCEEEEHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHCCCCCCCCCEEE
ILTKGRMGETYLIGADGEKNNKEVLEDILTRMGKDKDDYDRVTDRAGHDLRYAIDNSKLR
EEECCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEECCCHHH
TELGWAPKHTDFESGLQATIDWYRENENWWKAEKEAVEAKYAETQKVLD
HHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TEFKNIVVTGGAGFIGSNFVHYVYNNHPDVHITVLDKLTYAGNVNNINMLFDSGRVELV
CCCCEEEEECCCCCCCCCEEEEEECCCCCEEEEEEECCHHCCCCCCEEEEEECCCEEEE
VGDIADPEIVDQVASKADAIVHYAAESHNDNSLKSQDEFIQTNFIGTYTLIQAARKYDLR
ECCCCCHHHHHHHHHHHHHEEEEHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCEE
FHHVSTDEVYGDLPYREDLPGHGEGEGEKFTDKTPYNPSSPYSSTKAASDLIVRAWVRSF
EEECCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
GLKATISNCSNNYGPFQHIEKFIPRQITNILSGIKPKLYGDGKNVRDWIHTEDHSSGVWT
CCEEEEHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHCCCCCCCCCEEE
ILTKGRMGETYLIGADGEKNNKEVLEDILTRMGKDKDDYDRVTDRAGHDLRYAIDNSKLR
EEECCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEECCCHHH
TELGWAPKHTDFESGLQATIDWYRENENWWKAEKEAVEAKYAETQKVLD
HHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12397186; 11796113 [H]