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The map label for this gene is rmlC

Identifier: 15672181

GI number: 15672181

Start: 200377

End: 201276

Strand: Direct

Name: rmlC

Synonym: L320

Alternate gene names: 15672181

Gene position: 200377-201276 (Clockwise)

Preceding gene: 15672180

Following gene: 15672182

Centisome position: 8.47

GC content: 39.56

Gene sequence:

>900_bases
ATGATTTTAATAACAGGTGGAAATGGACAACTTGGAACAGAGTTGCGTCATTTACTTGATGAACGTGGCATGGCTTATAC
AGCAACAGATGCTAAAGAACTCGATATTACTGACGGCGTCGCTGTTGATAAATTCTTTGATGAAAATAAACCTGAACTTG
TCTATCATTGTGCGGCTTATACAGCTGTTGATAAAGCTGAAGACGAAGGTCGTGAACTTGATGAAAAAATCAATGTTGAT
GGAACTAAAAATGTAGCCGAAGCGGCAGCACGTGTTGGGGCAACACTTGTTTATATTTCAACTGACTATGTTTTTGGCGG
AACTTTGCCAATTGGCCAAGAGTGGCCGGTTGATGCACCAAAAGCTCCTGAATCTGAATATGGACGGACTAAACATCTTG
GTGAAGAAGCAGTTGTGGCTTCTGGTGTAAAACATTACATCATCCGTACTGCTTGGGTTTTTGGTTCTTATGGACCAAAC
TTTGTCTTCACAATGCAAAACTTGGCGACACGTTTTCCAGAATTAACCGTTGTTAATGATCAACACGGCAGACCAACTTG
GACACGTACACTTGCAGAATTTATGGTTTATTTGGTTAGTGAAAAAGCTGATTTTGGTTTTTATCATCTAACAAACGATG
CTGCGCCTGGTGAAGATGTGACTTGGTTTGATTTTGCCAAAGAAATCTTGAAAGACACAGATGTGCTTGTTAAACCTGTG
GATTCAAGCAAATTCCCGGCAAAAGCAAAACGTCCATTTAATTCAACAATGAGTCTTGAAAAAGCAAAAGCGACAGGTTT
TGTCATTCCAACATGGCAAGAAGCTTTAGCAAGTATGCTTGAAAAAGGCGATTTGCGTGAAAATAAAGATGGCGTAAAAG
GCGAAATTAACAAAAAATAA

Upstream 100 bases:

>100_bases
GTTTTGGATTAATAAAATAAATTCCAATTATATTATCTAAAAGTTACTGACAGTAATAATGAAAAAATCTGTCAGTAAAA
AATTAAAGATTGGAGAACAA

Downstream 100 bases:

>100_bases
TGACTCTGTCACTAAAATAAACTAATTGAAAATTCTGTCAGCGACCTGCAAAAGCTCGTCAGTACTGACAGAATTCTCAT
TTTGAGAGGCTGAAATAATC

Product: dTDP-4-dehydrorhamnose reductase

Products: NA

Alternate protein names: dTDP-4-keto-L-rhamnose reductase; dTDP-6-deoxy-L-lyxo-4-hexulose reductase; dTDP-6-deoxy-L-mannose dehydrogenase; dTDP-L-rhamnose synthase [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MILITGGNGQLGTELRHLLDERGMAYTATDAKELDITDGVAVDKFFDENKPELVYHCAAYTAVDKAEDEGRELDEKINVD
GTKNVAEAAARVGATLVYISTDYVFGGTLPIGQEWPVDAPKAPESEYGRTKHLGEEAVVASGVKHYIIRTAWVFGSYGPN
FVFTMQNLATRFPELTVVNDQHGRPTWTRTLAEFMVYLVSEKADFGFYHLTNDAAPGEDVTWFDFAKEILKDTDVLVKPV
DSSKFPAKAKRPFNSTMSLEKAKATGFVIPTWQEALASMLEKGDLRENKDGVKGEINKK

Sequences:

>Translated_299_residues
MILITGGNGQLGTELRHLLDERGMAYTATDAKELDITDGVAVDKFFDENKPELVYHCAAYTAVDKAEDEGRELDEKINVD
GTKNVAEAAARVGATLVYISTDYVFGGTLPIGQEWPVDAPKAPESEYGRTKHLGEEAVVASGVKHYIIRTAWVFGSYGPN
FVFTMQNLATRFPELTVVNDQHGRPTWTRTLAEFMVYLVSEKADFGFYHLTNDAAPGEDVTWFDFAKEILKDTDVLVKPV
DSSKFPAKAKRPFNSTMSLEKAKATGFVIPTWQEALASMLEKGDLRENKDGVKGEINKK
>Mature_299_residues
MILITGGNGQLGTELRHLLDERGMAYTATDAKELDITDGVAVDKFFDENKPELVYHCAAYTAVDKAEDEGRELDEKINVD
GTKNVAEAAARVGATLVYISTDYVFGGTLPIGQEWPVDAPKAPESEYGRTKHLGEEAVVASGVKHYIIRTAWVFGSYGPN
FVFTMQNLATRFPELTVVNDQHGRPTWTRTLAEFMVYLVSEKADFGFYHLTNDAAPGEDVTWFDFAKEILKDTDVLVKPV
DSSKFPAKAKRPFNSTMSLEKAKATGFVIPTWQEALASMLEKGDLRENKDGVKGEINKK

Specific function: Involved in the biosynthesis of the streptose moiety of streptomycin. Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4- hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well [H]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI11034825, Length=292, Percent_Identity=28.4246575342466, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI33519455, Length=292, Percent_Identity=28.4246575342466, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1788352, Length=299, Percent_Identity=34.1137123745819, Blast_Score=141, Evalue=5e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: =1.1.1.133 [H]

Molecular weight: Translated: 33098; Mature: 33098

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILITGGNGQLGTELRHLLDERGMAYTATDAKELDITDGVAVDKFFDENKPELVYHCAAY
CEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHCCCCCCEEEEHHHH
TAVDKAEDEGRELDEKINVDGTKNVAEAAARVGATLVYISTDYVFGGTLPIGQEWPVDAP
HHHHHCHHHHHHHHHHCCCCCCHHHHHHHHHHCEEEEEEEECEEECCCCCCCCCCCCCCC
KAPESEYGRTKHLGEEAVVASGVKHYIIRTAWVFGSYGPNFVFTMQNLATRFPELTVVND
CCCCHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHCCCEEEEEC
QHGRPTWTRTLAEFMVYLVSEKADFGFYHLTNDAAPGEDVTWFDFAKEILKDTDVLVKPV
CCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEECC
DSSKFPAKAKRPFNSTMSLEKAKATGFVIPTWQEALASMLEKGDLRENKDGVKGEINKK
CCCCCCCHHCCCCCCHHHHHHHHHCCEEEECHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MILITGGNGQLGTELRHLLDERGMAYTATDAKELDITDGVAVDKFFDENKPELVYHCAAY
CEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHCCCCCCEEEEHHHH
TAVDKAEDEGRELDEKINVDGTKNVAEAAARVGATLVYISTDYVFGGTLPIGQEWPVDAP
HHHHHCHHHHHHHHHHCCCCCCHHHHHHHHHHCEEEEEEEECEEECCCCCCCCCCCCCCC
KAPESEYGRTKHLGEEAVVASGVKHYIIRTAWVFGSYGPNFVFTMQNLATRFPELTVVND
CCCCHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHCCCEEEEEC
QHGRPTWTRTLAEFMVYLVSEKADFGFYHLTNDAAPGEDVTWFDFAKEILKDTDVLVKPV
CCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEECC
DSSKFPAKAKRPFNSTMSLEKAKATGFVIPTWQEALASMLEKGDLRENKDGVKGEINKK
CCCCCCCHHCCCCCCHHHHHHHHHCCEEEECHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1661369 [H]