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The map label for this gene is cspE

Identifier: 15672150

GI number: 15672150

Start: 172501

End: 172698

Strand: Reverse

Name: cspE

Synonym: L172505

Alternate gene names: 15672150

Gene position: 172698-172501 (Counterclockwise)

Preceding gene: 15672165

Following gene: 15672149

Centisome position: 7.3

GC content: 40.91

Gene sequence:

>198_bases
ATGGCACAAGGAACTGTTAAATGGTTTAACGCAACTAAAGGCTTCGGCTTTATCACTACTGAAGAAGGAAACGACGTTTT
CGCTCACTTCTCAGCTATCCAAACTGATGGATTCAAAACACTTGACGAAGGTCAAAAAGTTACTTTTGACGTTGAAGATG
GCCCTCGTGGACCTCAAGCTGTTAACATCCAAAAATAA

Upstream 100 bases:

>100_bases
GAAAGTGTAAGTTTTTGTTTTACGATTAGTTCTGTGCTTTTGGGTTCATTCCTATTCAAGAAATTAGATTTACAAATTTA
TAAATAAGGAAGTACCAAAA

Downstream 100 bases:

>100_bases
TTACAGCTTTAAATAAAAATCACTGACAGTTTTGTCAGTGATTTTTTTATTGTCAATTTTACTGATAGACTATTTTTTCG
TAAGCCTTTACAAATAATTC

Product: cold shock protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 65; Mature: 64

Protein sequence:

>65_residues
MAQGTVKWFNATKGFGFITTEEGNDVFAHFSAIQTDGFKTLDEGQKVTFDVEDGPRGPQAVNIQK

Sequences:

>Translated_65_residues
MAQGTVKWFNATKGFGFITTEEGNDVFAHFSAIQTDGFKTLDEGQKVTFDVEDGPRGPQAVNIQK
>Mature_64_residues
AQGTVKWFNATKGFGFITTEEGNDVFAHFSAIQTDGFKTLDEGQKVTFDVEDGPRGPQAVNIQK

Specific function: Binds To And Stimulates The Transcription Of The CCAAT- Containing, Cold-Shock-Inducible Promoters Of The H-Ns And Gyra Proteins. Binds Also To The Inverted Repeat 5'-ATTGG-3'. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1789979, Length=61, Percent_Identity=67.2131147540984, Blast_Score=87, Evalue=2e-19,
Organism=Escherichia coli, GI1786841, Length=62, Percent_Identity=64.5161290322581, Blast_Score=87, Evalue=2e-19,
Organism=Escherichia coli, GI1788126, Length=62, Percent_Identity=64.5161290322581, Blast_Score=84, Evalue=2e-18,
Organism=Escherichia coli, GI1787839, Length=61, Percent_Identity=63.9344262295082, Blast_Score=82, Evalue=6e-18,
Organism=Escherichia coli, GI2367114, Length=61, Percent_Identity=62.2950819672131, Blast_Score=81, Evalue=1e-17,
Organism=Escherichia coli, GI1787834, Length=61, Percent_Identity=62.2950819672131, Blast_Score=81, Evalue=2e-17,
Organism=Escherichia coli, GI1787107, Length=64, Percent_Identity=59.375, Blast_Score=79, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7124; Mature: 6993

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQGTVKWFNATKGFGFITTEEGNDVFAHFSAIQTDGFKTLDEGQKVTFDVEDGPRGPQA
CCCCCEEEEECCCCCEEEEECCCCEEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCEE
VNIQK
EEECC
>Mature Secondary Structure 
AQGTVKWFNATKGFGFITTEEGNDVFAHFSAIQTDGFKTLDEGQKVTFDVEDGPRGPQA
CCCCEEEEECCCCCEEEEECCCCEEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCEE
VNIQK
EEECC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]