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Identifier: 15834773

GI number: 15834773

Start: 185281

End: 185910

Strand: Reverse

Name: Not Available

Synonym: TC0153

Alternate gene names: 15834773

Gene position: 185910-185281 (Counterclockwise)

Preceding gene: 15834775

Following gene: 15834768

Centisome position: 17.33

GC content: 38.73

Gene sequence:

>630_bases
ATGTCTAAAACACCATTATCTATAGTCCATCCTTGGCATGGACCAGTATTAACACGCGATGATTACGAATCTCTTTGCTG
CTATATAGAGATCACTCCCTCTGATTCGGTTAAATTTGAATTGGATAAAGAGACGGGGTTACTAAAAGTCGATCGGCCAC
AGAAGTTTTCCAATTTTTGCCCTTGCCTATATGGCCTTTTACCTAAAACTTATTGTGGGGATCTTTCTGGGGAATATAGT
GGCCAGCAAAGCAATAGAGACAACATCAAAGGAGATGGAGACCCTCTTGATATTTGTGTACTAACAGAAAAGAATATCAC
CCAAGGAAATATCCTGTTGCAAGCTCGACCTATTGGAGGTATTCGTATTTTAGATTCTGGAGAAGCTGATGATAAAATTA
TTGCTGTTCTAGAAGATGATCTCGTTTACGGAGCTATGGAAGATATTTCTGACTGCCCCGGATCTGTTTTGGACATGATT
CAGCACTATTTTTTAACGTATAAGGCCACTCCTGAAAGTTTAATCCAAGCCAAACCTGCTAAAATTGAAATTATCGGCTT
ATATGGGAAAAAAGAAGCCCAAAAAGTCATCCGACTAGCGCACGAAGATTATTGTAATCTTTTTATGTAA

Upstream 100 bases:

>100_bases
ATAAAAGTTTGTGAGCCAACAAGAATGCTAAAACTATTGGACGAATAAGCTGTTAGCGCAGGATAATTGCTCGCAAAACA
CTTTAATAGAGAGTTATCTC

Downstream 100 bases:

>100_bases
AGCGGAGTAAAGAAAAGGCTATTATGAGATTTCTCATAATAGCCCTTCCTAATCAAGTTTTTTTCATCCTAGGGAACTTT
ATGAAGCAAATAGATAGTTT

Product: inorganic pyrophosphatase

Products: NA

Alternate protein names: Pyrophosphate phospho-hydrolase; PPase

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MSKTPLSIVHPWHGPVLTRDDYESLCCYIEITPSDSVKFELDKETGLLKVDRPQKFSNFCPCLYGLLPKTYCGDLSGEYS
GQQSNRDNIKGDGDPLDICVLTEKNITQGNILLQARPIGGIRILDSGEADDKIIAVLEDDLVYGAMEDISDCPGSVLDMI
QHYFLTYKATPESLIQAKPAKIEIIGLYGKKEAQKVIRLAHEDYCNLFM

Sequences:

>Translated_209_residues
MSKTPLSIVHPWHGPVLTRDDYESLCCYIEITPSDSVKFELDKETGLLKVDRPQKFSNFCPCLYGLLPKTYCGDLSGEYS
GQQSNRDNIKGDGDPLDICVLTEKNITQGNILLQARPIGGIRILDSGEADDKIIAVLEDDLVYGAMEDISDCPGSVLDMI
QHYFLTYKATPESLIQAKPAKIEIIGLYGKKEAQKVIRLAHEDYCNLFM
>Mature_208_residues
SKTPLSIVHPWHGPVLTRDDYESLCCYIEITPSDSVKFELDKETGLLKVDRPQKFSNFCPCLYGLLPKTYCGDLSGEYSG
QQSNRDNIKGDGDPLDICVLTEKNITQGNILLQARPIGGIRILDSGEADDKIIAVLEDDLVYGAMEDISDCPGSVLDMIQ
HYFLTYKATPESLIQAKPAKIEIIGLYGKKEAQKVIRLAHEDYCNLFM

Specific function: Unknown

COG id: COG0221

COG function: function code C; Inorganic pyrophosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PPase family

Homologues:

Organism=Escherichia coli, GI1790673, Length=182, Percent_Identity=29.6703296703297, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: 5480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): IPYR_CHLMU (Q9PLF1)

Other databases:

- EMBL:   AE002160
- PIR:   A81736
- RefSeq:   NP_296532.1
- ProteinModelPortal:   Q9PLF1
- SMR:   Q9PLF1
- GeneID:   1245687
- GenomeReviews:   AE002160_GR
- KEGG:   cmu:TC0153
- TIGR:   TC_0153
- HOGENOM:   HBG529150
- OMA:   TEKNITH
- ProtClustDB:   PRK00642
- BioCyc:   CMUR243161:TC_0153-MONOMER
- BRENDA:   3.6.1.1
- GO:   GO:0005737
- HAMAP:   MF_00209
- InterPro:   IPR008162
- Gene3D:   G3DSA:3.90.80.10
- PANTHER:   PTHR10286

Pfam domain/function: PF00719 Pyrophosphatase; SSF50324 Pyrophosphatase

EC number: =3.6.1.1

Molecular weight: Translated: 23310; Mature: 23179

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00387 PPASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTPLSIVHPWHGPVLTRDDYESLCCYIEITPSDSVKFELDKETGLLKVDRPQKFSNFC
CCCCCCEEECCCCCCEECCCCCCCEEEEEEECCCCCEEEEECCCCCEEEECCCCCHHHHH
PCLYGLLPKTYCGDLSGEYSGQQSNRDNIKGDGDPLDICVLTEKNITQGNILLQARPIGG
HHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCC
IRILDSGEADDKIIAVLEDDLVYGAMEDISDCPGSVLDMIQHYFLTYKATPESLIQAKPA
EEEEECCCCCCEEEEEEECCHHHHCHHHHHCCCHHHHHHHHHHHEEEECCHHHHHHCCCC
KIEIIGLYGKKEAQKVIRLAHEDYCNLFM
EEEEEEECCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKTPLSIVHPWHGPVLTRDDYESLCCYIEITPSDSVKFELDKETGLLKVDRPQKFSNFC
CCCCCEEECCCCCCEECCCCCCCEEEEEEECCCCCEEEEECCCCCEEEECCCCCHHHHH
PCLYGLLPKTYCGDLSGEYSGQQSNRDNIKGDGDPLDICVLTEKNITQGNILLQARPIGG
HHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCC
IRILDSGEADDKIIAVLEDDLVYGAMEDISDCPGSVLDMIQHYFLTYKATPESLIQAKPA
EEEEECCCCCCEEEEEEECCHHHHCHHHHHCCCHHHHHHHHHHHEEEECCHHHHHHCCCC
KIEIIGLYGKKEAQKVIRLAHEDYCNLFM
EEEEEEECCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10684935