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The map label for this gene is cls

Identifier: 13358153

GI number: 13358153

Start: 735951

End: 737549

Strand: Reverse

Name: cls

Synonym: UU588

Alternate gene names: 13358153

Gene position: 737549-735951 (Counterclockwise)

Preceding gene: 13358154

Following gene: 13358152

Centisome position: 98.11

GC content: 24.33

Gene sequence:

>1599_bases
ATGAAAAAACGTATTTTAGCCAAATTCATCATAACATTTATTTGTGTATTATTAATAATTAATATTTGTTTAATATCAAT
ATATTTTCACCATCGACCAACGGTTATTGTTACAGATATTTTAATATCATTTTATATTTTAAATACTAGTATTGGCTTAT
ATATTTTATCTTCAAGACATCGGCAAGATCGTTCTAAAAAATCATGATTGTTTATTTTTTTATTTCTACCAATTGTGTCA
ATTATATTCTTTTGGATTTTTGGATCACGCCCATTTAAAGATAAAAAAATTAATGATATCATTACAAACCGTCGTGCACT
TTTTAAATTTGAAGATTATGAATTTTATCATAGTTTTCAAAATATGATTATTAATAATAATGGACTTAATTTAATTCGTA
AAATTGGTCAGTATAATTACCAACTTTTTCGTACACCTATTTATTGTGATAATCAAATAACAATTATTAAAGAGACAAAC
GAAATGTATGAACAAACTATTAAATTAATAAGAAAAGCAAAAAAAACAATTTATTTACAATATTATATTATTGAGAATGG
TCGTTGATTACAATCTCTTGTTAAAGAATTAATAAAAAAAAGTAATGATGGTATAAAAATATATATATTATATGACCCAA
TTGGTTCGTTAAATAAATTTCCTAAAAAATATCGTCAACAGTTAATTAATAATGGTATAGAAATTGCTTGTTTTAAAGCA
CGTGCAGATTATAAATTTAGAAGTACTATTAATTTTCGTTCGCATCGAAAATATTTAATTATTGATGGTGAAGTTGCTAT
TAGTGGCGGCTCTAATATTGGTGATTCTTATTTAAATATTGATCCTAAGGTTGGTCATTGAGTTGATTTGAATTATATTT
TAACCGGAGGAATTGTGCATACAATTCTTTTAGAATTTATTCATGATTGATACTACTATACACCATTTTCAAAAAAGAAT
AATTTTAATGTTGTTGTTGACAGTATGCATCCTCAACCTTACTTATGTCAAGGAAAAGATGACTTAATAAATAATTCTAT
TAAAAATAAAACAATAACAACACTTTTGCATACAGGACCAGACCAATCAACAAGTTCTTTAAATGAAATTCTGGTTATGG
CAATCGCTAATGCTAAAAAATCAATTACAATTTTTACGCCATATTTATTTCCTACTGAAGATATTTTAATAGGACTTAAA
GCTGCAGCGAATACTGGAATAAAAGTTAAAATTATTTTACCTGGAAGGGTTGATTCTTGAAGTTTTATCATATCAATGAA
TCGTTTAAGTTATGAATCATTAATTAAGTCCGGTGTACAAATTTATGAATATAGTGGTTTTTTACACACTAAAGCGATTT
TAATAGATGATGATATTTCACTATTAGGTTCTTATAATCTTGATAATAGAGCATTAGTAATTAATTACGAATCATTATTA
ACAATTTATTCAATACAAGTAAATCACGACATTAACGAGATTTTTAATCAATATCTCCGTAATTCACAGTTAATTAATTT
AGAAGATTTAGATAACAAATTCACATTCAACGAATTAGTTCAAACAGTATTTATTAATATTTTTCAATTACTTTTATAA

Upstream 100 bases:

>100_bases
TCGAATCCCACTGTTTCCGCCATTAATAATTAAAAATAGGGGAAACCCTATTTTTTATTTATATAAATTATAATTATTAA
TAAAAAGGACGATAATAAGT

Downstream 100 bases:

>100_bases
TAAAAGTTATTATATTAATAATATAAGATATCAAGACAATGCTTTAAGTAATATAATTAAAATACATTTAAAAAATGATA
TTTTATGTAAAGGTTTAATA

Product: cardiolipin synthase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MKKRILAKFIITFICVLLIINICLISIYFHHRPTVIVTDILISFYILNTSIGLYILSSRHRQDRSKKSWLFIFLFLPIVS
IIFFWIFGSRPFKDKKINDIITNRRALFKFEDYEFYHSFQNMIINNNGLNLIRKIGQYNYQLFRTPIYCDNQITIIKETN
EMYEQTIKLIRKAKKTIYLQYYIIENGRWLQSLVKELIKKSNDGIKIYILYDPIGSLNKFPKKYRQQLINNGIEIACFKA
RADYKFRSTINFRSHRKYLIIDGEVAISGGSNIGDSYLNIDPKVGHWVDLNYILTGGIVHTILLEFIHDWYYYTPFSKKN
NFNVVVDSMHPQPYLCQGKDDLINNSIKNKTITTLLHTGPDQSTSSLNEILVMAIANAKKSITIFTPYLFPTEDILIGLK
AAANTGIKVKIILPGRVDSWSFIISMNRLSYESLIKSGVQIYEYSGFLHTKAILIDDDISLLGSYNLDNRALVINYESLL
TIYSIQVNHDINEIFNQYLRNSQLINLEDLDNKFTFNELVQTVFINIFQLLL

Sequences:

>Translated_532_residues
MKKRILAKFIITFICVLLIINICLISIYFHHRPTVIVTDILISFYILNTSIGLYILSSRHRQDRSKKS*LFIFLFLPIVS
IIFFWIFGSRPFKDKKINDIITNRRALFKFEDYEFYHSFQNMIINNNGLNLIRKIGQYNYQLFRTPIYCDNQITIIKETN
EMYEQTIKLIRKAKKTIYLQYYIIENGR*LQSLVKELIKKSNDGIKIYILYDPIGSLNKFPKKYRQQLINNGIEIACFKA
RADYKFRSTINFRSHRKYLIIDGEVAISGGSNIGDSYLNIDPKVGH*VDLNYILTGGIVHTILLEFIHD*YYYTPFSKKN
NFNVVVDSMHPQPYLCQGKDDLINNSIKNKTITTLLHTGPDQSTSSLNEILVMAIANAKKSITIFTPYLFPTEDILIGLK
AAANTGIKVKIILPGRVDS*SFIISMNRLSYESLIKSGVQIYEYSGFLHTKAILIDDDISLLGSYNLDNRALVINYESLL
TIYSIQVNHDINEIFNQYLRNSQLINLEDLDNKFTFNELVQTVFINIFQLLL
>Mature_532_residues
MKKRILAKFIITFICVLLIINICLISIYFHHRPTVIVTDILISFYILNTSIGLYILSSRHRQDRSKKS*LFIFLFLPIVS
IIFFWIFGSRPFKDKKINDIITNRRALFKFEDYEFYHSFQNMIINNNGLNLIRKIGQYNYQLFRTPIYCDNQITIIKETN
EMYEQTIKLIRKAKKTIYLQYYIIENGR*LQSLVKELIKKSNDGIKIYILYDPIGSLNKFPKKYRQQLINNGIEIACFKA
RADYKFRSTINFRSHRKYLIIDGEVAISGGSNIGDSYLNIDPKVGH*VDLNYILTGGIVHTILLEFIHD*YYYTPFSKKN
NFNVVVDSMHPQPYLCQGKDDLINNSIKNKTITTLLHTGPDQSTSSLNEILVMAIANAKKSITIFTPYLFPTEDILIGLK
AAANTGIKVKIILPGRVDS*SFIISMNRLSYESLIKSGVQIYEYSGFLHTKAILIDDDISLLGSYNLDNRALVINYESLL
TIYSIQVNHDINEIFNQYLRNSQLINLEDLDNKFTFNELVQTVFINIFQLLL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=505, Percent_Identity=23.960396039604, Blast_Score=152, Evalue=7e-38,
Organism=Escherichia coli, GI1787007, Length=354, Percent_Identity=25.9887005649718, Blast_Score=98, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 61057; Mature: 61057

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRILAKFIITFICVLLIINICLISIYFHHRPTVIVTDILISFYILNTSIGLYILSSRH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEECHH
RQDRSKKSLFIFLFLPIVSIIFFWIFGSRPFKDKKINDIITNRRALFKFEDYEFYHSFQN
HCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCEEEEEECCHHHHHHHHH
MIINNNGLNLIRKIGQYNYQLFRTPIYCDNQITIIKETNEMYEQTIKLIRKAKKTIYLQY
HEECCCCHHHHHHHHCCCEEEEECCEEECCEEEEEECCHHHHHHHHHHHHHHCCEEEEEE
YIIENGRLQSLVKELIKKSNDGIKIYILYDPIGSLNKFPKKYRQQLINNGIEIACFKARA
EEECCCCHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEECC
DYKFRSTINFRSHRKYLIIDGEVAISGGSNIGDSYLNIDPKVGHVDLNYILTGGIVHTIL
CEEEEEEECCCCCCEEEEEECEEEEECCCCCCCCCEECCCCCCEEEEEEEEEHHHHHHHH
LEFIHDYYYTPFSKKNNFNVVVDSMHPQPYLCQGKDDLINNSIKNKTITTLLHTGPDQST
HHHHHHHHCCCCCCCCCCEEEEECCCCCCEEECCCHHHHCCCCCCCEEEEEEECCCCCCH
SSLNEILVMAIANAKKSITIFTPYLFPTEDILIGLKAAANTGIKVKIILPGRVDSSFIIS
HHHHHHHHEEEECCCCEEEEEECCCCCCHHHEEEEEHHCCCCEEEEEEECCCCCCEEEEE
MNRLSYESLIKSGVQIYEYSGFLHTKAILIDDDISLLGSYNLDNRALVINYESLLTIYSI
EHHCCHHHHHHCCCEEEEECCEEEEEEEEEECCHHEEECCCCCCCEEEEEHHHEEEEEEE
QVNHDINEIFNQYLRNSQLINLEDLDNKFTFNELVQTVFINIFQLLL
EECCCHHHHHHHHHCCCCEEEHHHCCCCCCHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MKKRILAKFIITFICVLLIINICLISIYFHHRPTVIVTDILISFYILNTSIGLYILSSRH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEECHH
RQDRSKKSLFIFLFLPIVSIIFFWIFGSRPFKDKKINDIITNRRALFKFEDYEFYHSFQN
HCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCEEEEEECCHHHHHHHHH
MIINNNGLNLIRKIGQYNYQLFRTPIYCDNQITIIKETNEMYEQTIKLIRKAKKTIYLQY
HEECCCCHHHHHHHHCCCEEEEECCEEECCEEEEEECCHHHHHHHHHHHHHHCCEEEEEE
YIIENGRLQSLVKELIKKSNDGIKIYILYDPIGSLNKFPKKYRQQLINNGIEIACFKARA
EEECCCCHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEECC
DYKFRSTINFRSHRKYLIIDGEVAISGGSNIGDSYLNIDPKVGHVDLNYILTGGIVHTIL
CEEEEEEECCCCCCEEEEEECEEEEECCCCCCCCCEECCCCCCEEEEEEEEEHHHHHHHH
LEFIHDYYYTPFSKKNNFNVVVDSMHPQPYLCQGKDDLINNSIKNKTITTLLHTGPDQST
HHHHHHHHCCCCCCCCCCEEEEECCCCCCEEECCCHHHHCCCCCCCEEEEEEECCCCCCH
SSLNEILVMAIANAKKSITIFTPYLFPTEDILIGLKAAANTGIKVKIILPGRVDSSFIIS
HHHHHHHHEEEECCCCEEEEEECCCCCCHHHEEEEEHHCCCCEEEEEEECCCCCCEEEEE
MNRLSYESLIKSGVQIYEYSGFLHTKAILIDDDISLLGSYNLDNRALVINYESLLTIYSI
EHHCCHHHHHHCCCEEEEECCEEEEEEEEEECCHHEEECCCCCCCEEEEEHHHEEEEEEE
QVNHDINEIFNQYLRNSQLINLEDLDNKFTFNELVQTVFINIFQLLL
EECCCHHHHHHHHHCCCCEEEHHHCCCCCCHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9443601 [H]