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The map label for this gene is dnaB

Identifier: 13358114

GI number: 13358114

Start: 684427

End: 685887

Strand: Reverse

Name: dnaB

Synonym: UU550

Alternate gene names: 13358114

Gene position: 685887-684427 (Counterclockwise)

Preceding gene: 13358115

Following gene: 13358113

Centisome position: 91.24

GC content: 26.9

Gene sequence:

>1461_bases
ATGCCAACAAAAGACTTAAATGATGCGATATTAGATTTGTTTTGTTTAATTATTAATAATAATGACTTTTGAAAAGATGT
TGTTTTACGTTTAGAAGTTAAAGATTTTCCAGAAAAGATTCAGCAAAATATTTTTAATACAATTGCTAATTTAAACGAAC
AAAAGTATAAAATCAATGAATCAAATATTTTAAATGCATTAGCTAATTATGTGATTATTGATGAACAAGATCAAAATTAT
TTATTACATAAGAATTATTTACTTCAAATTTTAGATCGTACTGATTATTTAGTAGATTTAAAAGATTGTATTGAAATTAT
TAAGAATGCTTCAATTAAAAATAAACTTGATGTTTTTGCAAATGAGATTTTAGCAACCCAAATTTCATTAATTAACGCTA
AAGATCAATTTAAAGAAATGCATGAAAAATTTTTAGAAATTCTTGCATCACGAACCGAAGATACTATTGAAAATATGGAA
TTAATTGCTAATCGATATTTTGAGAAATTAAATAAAATAGGCAATAGCGGGATTATTCCTGGAGTTATTAAAACTAAATA
CGATAATATTGATAAATTCACTAATGGTTATAAACCTGGAGAATTAGTTGTTATTGCCGCTCGTCCTGGAATTGGAAAAA
CTACATTTTGTTTAAATGTTATGGTTAACAATGTTAATGAAATTATTGAATATAACGAAAATATTCAACCAAATCAAAAA
GAAAAGATCATTGTAATGTTTTCGCTTGAAATTACTAAAGAACAAATTTTACAAAAGTTTATCTCAATTAAAACAGGAAT
TTCTAATCGTGAAGTTATAGAAAATAAATATCGTATAACTAAAGGATATGACACAAGGTCTTTTGCTATGCAAGCAATCA
ATGATATTAAAAGTTGACCTATTTTTATCGATGATCGTCCTAATATTTCAATTGTAGACATTGAAGCAAAATTATATGAT
TTAAAAAAACGTTATGATATTGCTTTAGTCGTTTTAGATTATTTACAATTGGTTTCAGGAGGCAATGCAAATAAAAATAT
GACAAGAACACAAGAGGTAGGTCGTGTTTCAAGTGCATTAAAAATCATTGCTAAAGAAATTAATGCACCAGTAATCGCTA
TCGCTCAACTTTCACGTAAAGCAGAAGAACGTGATGTAAGCAATAATGTTAATATGAAAAATAACCCTTTAGTAAAAACA
ATTGATAATTCCCCAAAATTGTCTGATTTGCGAGAATCTGGATCAATTGAACAAGATGCTGACGTTGTTGCTTTTTTACA
TTGGGATCGAAAACAACGCAATTTTATGCAAAATGATAATCAAGAAACCCGAATGCGCGATGATTTGATTGAAGCAAAAT
TTATTGTTGAAAAGAACCGAAACGGGTCAACAGGTGAAACTGATATTATTTTTTCTAAATTAAATAGTAAATTTATTCGT
GCAACCACTTCAAAGGAGTAA

Upstream 100 bases:

>100_bases
AACCACATGCATTAGGTATTGGTGAACATATTGTTGAAATTTTATTGCATAAAAAGGTTACTGCTAAACTTCATGTTATT
GTTAGCAAAGAATAATAATT

Downstream 100 bases:

>100_bases
ATATGAAAAAAAACAAAAGTAAAAAGCTAATTTTTTCAATATTAGGAATTAGTTTTATTGGATTAACGCCAATTGTTGCT
GCATCGTGTTCAGCAGCTGC

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MPTKDLNDAILDLFCLIINNNDFWKDVVLRLEVKDFPEKIQQNIFNTIANLNEQKYKINESNILNALANYVIIDEQDQNY
LLHKNYLLQILDRTDYLVDLKDCIEIIKNASIKNKLDVFANEILATQISLINAKDQFKEMHEKFLEILASRTEDTIENME
LIANRYFEKLNKIGNSGIIPGVIKTKYDNIDKFTNGYKPGELVVIAARPGIGKTTFCLNVMVNNVNEIIEYNENIQPNQK
EKIIVMFSLEITKEQILQKFISIKTGISNREVIENKYRITKGYDTRSFAMQAINDIKSWPIFIDDRPNISIVDIEAKLYD
LKKRYDIALVVLDYLQLVSGGNANKNMTRTQEVGRVSSALKIIAKEINAPVIAIAQLSRKAEERDVSNNVNMKNNPLVKT
IDNSPKLSDLRESGSIEQDADVVAFLHWDRKQRNFMQNDNQETRMRDDLIEAKFIVEKNRNGSTGETDIIFSKLNSKFIR
ATTSKE

Sequences:

>Translated_486_residues
MPTKDLNDAILDLFCLIINNNDF*KDVVLRLEVKDFPEKIQQNIFNTIANLNEQKYKINESNILNALANYVIIDEQDQNY
LLHKNYLLQILDRTDYLVDLKDCIEIIKNASIKNKLDVFANEILATQISLINAKDQFKEMHEKFLEILASRTEDTIENME
LIANRYFEKLNKIGNSGIIPGVIKTKYDNIDKFTNGYKPGELVVIAARPGIGKTTFCLNVMVNNVNEIIEYNENIQPNQK
EKIIVMFSLEITKEQILQKFISIKTGISNREVIENKYRITKGYDTRSFAMQAINDIKS*PIFIDDRPNISIVDIEAKLYD
LKKRYDIALVVLDYLQLVSGGNANKNMTRTQEVGRVSSALKIIAKEINAPVIAIAQLSRKAEERDVSNNVNMKNNPLVKT
IDNSPKLSDLRESGSIEQDADVVAFLHWDRKQRNFMQNDNQETRMRDDLIEAKFIVEKNRNGSTGETDIIFSKLNSKFIR
ATTSKE
>Mature_485_residues
PTKDLNDAILDLFCLIINNNDF*KDVVLRLEVKDFPEKIQQNIFNTIANLNEQKYKINESNILNALANYVIIDEQDQNYL
LHKNYLLQILDRTDYLVDLKDCIEIIKNASIKNKLDVFANEILATQISLINAKDQFKEMHEKFLEILASRTEDTIENMEL
IANRYFEKLNKIGNSGIIPGVIKTKYDNIDKFTNGYKPGELVVIAARPGIGKTTFCLNVMVNNVNEIIEYNENIQPNQKE
KIIVMFSLEITKEQILQKFISIKTGISNREVIENKYRITKGYDTRSFAMQAINDIKS*PIFIDDRPNISIVDIEAKLYDL
KKRYDIALVVLDYLQLVSGGNANKNMTRTQEVGRVSSALKIIAKEINAPVIAIAQLSRKAEERDVSNNVNMKNNPLVKTI
DNSPKLSDLRESGSIEQDADVVAFLHWDRKQRNFMQNDNQETRMRDDLIEAKFIVEKNRNGSTGETDIIFSKLNSKFIRA
TTSKE

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity [H]

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=475, Percent_Identity=26.7368421052632, Blast_Score=145, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694 [H]

Pfam domain/function: PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 55522; Mature: 55391

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTKDLNDAILDLFCLIINNNDFKDVVLRLEVKDFPEKIQQNIFNTIANLNEQKYKINES
CCCCCHHHHHHHHHHHHCCCCCHHHEEEEEEHHHHHHHHHHHHHHHHHCCCCHHEECCHH
NILNALANYVIIDEQDQNYLLHKNYLLQILDRTDYLVDLKDCIEIIKNASIKNKLDVFAN
HHHHHHHCEEEEECCCCCEEEHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCHHHHHHHHH
EILATQISLINAKDQFKEMHEKFLEILASRTEDTIENMELIANRYFEKLNKIGNSGIIPG
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
VIKTKYDNIDKFTNGYKPGELVVIAARPGIGKTTFCLNVMVNNVNEIIEYNENIQPNQKE
HHHCCCCCHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
KIIVMFSLEITKEQILQKFISIKTGISNREVIENKYRITKGYDTRSFAMQAINDIKSPIF
EEEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHEEECCCCHHHHHHHHHHHCCCCEE
IDDRPNISIVDIEAKLYDLKKRYDIALVVLDYLQLVSGGNANKNMTRTQEVGRVSSALKI
ECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
IAKEINAPVIAIAQLSRKAEERDVSNNVNMKNNPLVKTIDNSPKLSDLRESGSIEQDADV
HHHHHCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCHHHHHHCCCCCCCCCE
VAFLHWDRKQRNFMQNDNQETRMRDDLIEAKFIVEKNRNGSTGETDIIFSKLNSKFIRAT
EEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHCCHHEECC
TSKE
CCCC
>Mature Secondary Structure 
PTKDLNDAILDLFCLIINNNDFKDVVLRLEVKDFPEKIQQNIFNTIANLNEQKYKINES
CCCCHHHHHHHHHHHHCCCCCHHHEEEEEEHHHHHHHHHHHHHHHHHCCCCHHEECCHH
NILNALANYVIIDEQDQNYLLHKNYLLQILDRTDYLVDLKDCIEIIKNASIKNKLDVFAN
HHHHHHHCEEEEECCCCCEEEHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCHHHHHHHHH
EILATQISLINAKDQFKEMHEKFLEILASRTEDTIENMELIANRYFEKLNKIGNSGIIPG
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
VIKTKYDNIDKFTNGYKPGELVVIAARPGIGKTTFCLNVMVNNVNEIIEYNENIQPNQKE
HHHCCCCCHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
KIIVMFSLEITKEQILQKFISIKTGISNREVIENKYRITKGYDTRSFAMQAINDIKSPIF
EEEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHEEECCCCHHHHHHHHHHHCCCCEE
IDDRPNISIVDIEAKLYDLKKRYDIALVVLDYLQLVSGGNANKNMTRTQEVGRVSSALKI
ECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
IAKEINAPVIAIAQLSRKAEERDVSNNVNMKNNPLVKTIDNSPKLSDLRESGSIEQDADV
HHHHHCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCHHHHHHCCCCCCCCCE
VAFLHWDRKQRNFMQNDNQETRMRDDLIEAKFIVEKNRNGSTGETDIIFSKLNSKFIRAT
EEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHCCHHEECC
TSKE
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7569993; 8253680 [H]