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The map label for this gene is gatB

Identifier: 13358108

GI number: 13358108

Start: 677822

End: 679255

Strand: Reverse

Name: gatB

Synonym: UU544

Alternate gene names: 13358108

Gene position: 679255-677822 (Counterclockwise)

Preceding gene: 13358109

Following gene: 13358105

Centisome position: 90.36

GC content: 26.71

Gene sequence:

>1434_bases
ATGCAAAATTTTGAAGTTATTATTGGTATTGAAGTGCATACTGCACTTAATACAAAAACCAAAATGTTTTCTAATGCAGC
AACATCGCATAAGAGTATTCCTAACACTTTAATTAATGAAATTGATTTAGCATTGCCAGGTACATTACCAACTGTTAATC
AAGAAGTTGTTCATAAAGGACTTTTTTTAGCAAATGCATTACACATGCGTACTAATCATCAATTTATTGCTTTTGATCGT
AAGCATTATTATTATTTAGACTTACCTAAAGGATATCAAATTACACAAAATTATTTTCCAATAGGTCAGAATGGTTATAT
TCAAATCATTGATGAATATAATAATTTAAAACGAATACGTATCAAACAAATTCATTTAGAAGAAGATACAGCCAAACAAA
CTAATATAGGTAATCAAATATATTTAGATTATAACCGTGCAGGATGACCTTTAATTGAAATCGTTAGTGAAGCAGATTTA
CGAAGTGCACAAGAGACGGTTTTATTTTTAGAAGAATTGCGAAAAATTTTATTGTTTAATGATATATCAGATGCAAAAAT
GGAAGATGGATCATTACGTGTTGATGTAAACGTTTCAATTAGACCTCAAGGGGCTAAAAAATTTGGCACAAAAGTAGAAA
TTAAAAATATTAATTCTATTTCAAATGTTGCTAAAGCTATTGATTATGAGATAAGACGTCAATTAAATTTAATTTTACTA
AATCAGAATGTTGAACAACAAACACGACGTTTTGATGATAATACGAATACAACGGTTTTTATGCGTTCAAAAAATGATGC
TATTAATTATCGCTATATTCGTGAAGCAAATATTGCACCAATTCATCTAAGTGATGATTATGTAAAAAAAATGTTTTTAA
CTAAATCATGTAGTATTAATGATTTGCGTCAACAACTAGCACAAAAAGGTTTAGTTAGTTCTGCTATCGAACAGTTATTA
AGTGATGGACCGTTGTTTAAAGCATTTAAATATGTAGACAAAATTGTTAATAATCCTTTAAGTGTTTATAAATGATTATG
TTTGGAATTTATTGGTCTAATTAATAAAAATACGCAAAACATTGAAGAAATTACACCAGAATTATTGCAAAAAATTGCTA
GAATGATCTTATTATTTGATCAAACTTTGATTAATGGAAAACAAACAAAAATAATTTTAGAAAAAATTTATTTGACAAAT
AAAGATCCTCAAATTCTAATTAAGGAATTAGGTTTTGAGCAAATTACTAATGAAAATGAAATTACTAAACTTTGACATCA
AATTTTAGCTAAGAATCAAGAAATGTTATTACAATACAATGAACGCCCCGACCGTGTTGAAAAATTTTTTATGGGTGAAA
TAATGAAATTAACTAAAGCACAAGCGAATCCAACCATTTCTTTTAATGTTTTAAAAAAAATCTTACAAAAATAA

Upstream 100 bases:

>100_bases
ATTTAGGTTTAAATTTGTGAGCAGATTACTTAAATGATAATAAATTATTAAATGGCGCTTATTTAATCAATAATTTACTA
GGAAATGAGGAAAAATAATA

Downstream 100 bases:

>100_bases
AAACAAGCAATTAACATTAAAAAAAAGACGGTCTTTACGGCCGTCTTTTTAATTATTAAATTGGTTTAAAGCTTTTGGTA
AATTTAGTTTTTTTGTTTTA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MQNFEVIIGIEVHTALNTKTKMFSNAATSHKSIPNTLINEIDLALPGTLPTVNQEVVHKGLFLANALHMRTNHQFIAFDR
KHYYYLDLPKGYQITQNYFPIGQNGYIQIIDEYNNLKRIRIKQIHLEEDTAKQTNIGNQIYLDYNRAGWPLIEIVSEADL
RSAQETVLFLEELRKILLFNDISDAKMEDGSLRVDVNVSIRPQGAKKFGTKVEIKNINSISNVAKAIDYEIRRQLNLILL
NQNVEQQTRRFDDNTNTTVFMRSKNDAINYRYIREANIAPIHLSDDYVKKMFLTKSCSINDLRQQLAQKGLVSSAIEQLL
SDGPLFKAFKYVDKIVNNPLSVYKWLCLEFIGLINKNTQNIEEITPELLQKIARMILLFDQTLINGKQTKIILEKIYLTN
KDPQILIKELGFEQITNENEITKLWHQILAKNQEMLLQYNERPDRVEKFFMGEIMKLTKAQANPTISFNVLKKILQK

Sequences:

>Translated_477_residues
MQNFEVIIGIEVHTALNTKTKMFSNAATSHKSIPNTLINEIDLALPGTLPTVNQEVVHKGLFLANALHMRTNHQFIAFDR
KHYYYLDLPKGYQITQNYFPIGQNGYIQIIDEYNNLKRIRIKQIHLEEDTAKQTNIGNQIYLDYNRAG*PLIEIVSEADL
RSAQETVLFLEELRKILLFNDISDAKMEDGSLRVDVNVSIRPQGAKKFGTKVEIKNINSISNVAKAIDYEIRRQLNLILL
NQNVEQQTRRFDDNTNTTVFMRSKNDAINYRYIREANIAPIHLSDDYVKKMFLTKSCSINDLRQQLAQKGLVSSAIEQLL
SDGPLFKAFKYVDKIVNNPLSVYK*LCLEFIGLINKNTQNIEEITPELLQKIARMILLFDQTLINGKQTKIILEKIYLTN
KDPQILIKELGFEQITNENEITKL*HQILAKNQEMLLQYNERPDRVEKFFMGEIMKLTKAQANPTISFNVLKKILQK
>Mature_477_residues
MQNFEVIIGIEVHTALNTKTKMFSNAATSHKSIPNTLINEIDLALPGTLPTVNQEVVHKGLFLANALHMRTNHQFIAFDR
KHYYYLDLPKGYQITQNYFPIGQNGYIQIIDEYNNLKRIRIKQIHLEEDTAKQTNIGNQIYLDYNRAG*PLIEIVSEADL
RSAQETVLFLEELRKILLFNDISDAKMEDGSLRVDVNVSIRPQGAKKFGTKVEIKNINSISNVAKAIDYEIRRQLNLILL
NQNVEQQTRRFDDNTNTTVFMRSKNDAINYRYIREANIAPIHLSDDYVKKMFLTKSCSINDLRQQLAQKGLVSSAIEQLL
SDGPLFKAFKYVDKIVNNPLSVYK*LCLEFIGLINKNTQNIEEITPELLQKIARMILLFDQTLINGKQTKIILEKIYLTN
KDPQILIKELGFEQITNENEITKL*HQILAKNQEMLLQYNERPDRVEKFFMGEIMKLTKAQANPTISFNVLKKILQK

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=504, Percent_Identity=30.7539682539683, Blast_Score=214, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI115534094, Length=301, Percent_Identity=38.8704318936877, Blast_Score=199, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6319391, Length=299, Percent_Identity=35.7859531772575, Blast_Score=168, Evalue=2e-42,
Organism=Drosophila melanogaster, GI45550808, Length=498, Percent_Identity=28.9156626506024, Blast_Score=198, Evalue=8e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 54667; Mature: 54667

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNFEVIIGIEVHTALNTKTKMFSNAATSHKSIPNTLINEIDLALPGTLPTVNQEVVHKG
CCCCEEEEEEEEEEHHCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHH
LFLANALHMRTNHQFIAFDRKHYYYLDLPKGYQITQNYFPIGQNGYIQIIDEYNNLKRIR
HHHHHHHHHCCCCEEEEEECCEEEEEECCCCCEEECCCCCCCCCCCEEEEECCCCCEEEE
IKQIHLEEDTAKQTNIGNQIYLDYNRAGPLIEIVSEADLRSAQETVLFLEELRKILLFND
EEEEEECCCCHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ISDAKMEDGSLRVDVNVSIRPQGAKKFGTKVEIKNINSISNVAKAIDYEIRRQLNLILLN
CCCCCCCCCCEEEEEEEEECCCCHHHCCCEEEEECCHHHHHHHHHHHHHHHHCEEEEEEC
QNVEQQTRRFDDNTNTTVFMRSKNDAINYRYIREANIAPIHLSDDYVKKMFLTKSCSIND
CCHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEECCCEEEECCHHHHHHHHHHCCCCHHH
LRQQLAQKGLVSSAIEQLLSDGPLFKAFKYVDKIVNNPLSVYKLCLEFIGLINKNTQNIE
HHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHH
EITPELLQKIARMILLFDQTLINGKQTKIILEKIYLTNKDPQILIKELGFEQITNENEIT
HHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEHHCCCCCHHHHHHHCCHHHHCCCHHHH
KLHQILAKNQEMLLQYNERPDRVEKFFMGEIMKLTKAQANPTISFNVLKKILQK
HHHHHHHCCHHHEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCC
>Mature Secondary Structure
MQNFEVIIGIEVHTALNTKTKMFSNAATSHKSIPNTLINEIDLALPGTLPTVNQEVVHKG
CCCCEEEEEEEEEEHHCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHH
LFLANALHMRTNHQFIAFDRKHYYYLDLPKGYQITQNYFPIGQNGYIQIIDEYNNLKRIR
HHHHHHHHHCCCCEEEEEECCEEEEEECCCCCEEECCCCCCCCCCCEEEEECCCCCEEEE
IKQIHLEEDTAKQTNIGNQIYLDYNRAGPLIEIVSEADLRSAQETVLFLEELRKILLFND
EEEEEECCCCHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ISDAKMEDGSLRVDVNVSIRPQGAKKFGTKVEIKNINSISNVAKAIDYEIRRQLNLILLN
CCCCCCCCCCEEEEEEEEECCCCHHHCCCEEEEECCHHHHHHHHHHHHHHHHCEEEEEEC
QNVEQQTRRFDDNTNTTVFMRSKNDAINYRYIREANIAPIHLSDDYVKKMFLTKSCSIND
CCHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEECCCEEEECCHHHHHHHHHHCCCCHHH
LRQQLAQKGLVSSAIEQLLSDGPLFKAFKYVDKIVNNPLSVYKLCLEFIGLINKNTQNIE
HHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHH
EITPELLQKIARMILLFDQTLINGKQTKIILEKIYLTNKDPQILIKELGFEQITNENEIT
HHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEHHCCCCCHHHHHHHCCHHHHCCCHHHH
KLHQILAKNQEMLLQYNERPDRVEKFFMGEIMKLTKAQANPTISFNVLKKILQK
HHHHHHHCCHHHEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA