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The map label for this gene is gatA

Identifier: 13358109

GI number: 13358109

Start: 679258

End: 680691

Strand: Reverse

Name: gatA

Synonym: UU545

Alternate gene names: 13358109

Gene position: 680691-679258 (Counterclockwise)

Preceding gene: 13358110

Following gene: 13358108

Centisome position: 90.55

GC content: 27.27

Gene sequence:

>1434_bases
ATGAAAAATAAAACTATTTTAGCGCTTCATAATCAAATTAAAAAACGTGAAGTTAAAATTTATGATTTGATTAAAAAATC
GATTGATCATGATTTAAAGATTAAAAATTTAAATGCAACTTTAGCAAATAATTATGAACAGGCATTGAAACAAGCAGCTT
TTTTAGATGAAAAGATTAGTAGTGATAATTTAGAAATTGATTATTTATTTGGAATTCCATATTCTTTAAAAGACAACATT
TCAACTAAGAATATTATTACAACAGGTGGTTCATTGTTTTTAAAAAATTACAATCCTCCATATGATGCGACAGTTAAAAC
ATTATTAGATCAAAAACAAGCAATTTTGTTAAATAAATCAAATTTAGATGAATTTGGTTTAGGAGGCACTGGTACTGATT
CAGCATTTGGCCCTGTAATTAATCCATTAAATCCAAAACATGTTAGCGGAGGTTCTTCATCTGGTAGTGCTGTTTTAGTT
CAACAAGGCGTTGTACCTTTTGCAATTGCTACAGATACAGGAGATTCTGTTCGTCGCCCCGCTTCAATTATGGGTATTGT
TGGTTTTAAACCGTCATATGGTCTTATTTCGCGTTATGGCGTTTACCCCTTTGCTCCTTCTCTTGATCATGTTGGAATTT
TTACAAATAATATTTTTGATACGCAAATTGTTTTTAATGCTCTTGCTAAGCGAGATTTTAAAGATTTTACAAGTCTTAGT
TTTAAAACACAATCACTTTCAATGCTAAAAACAAAAAATAAATATAAGATTGGTGTTTTAAAACCTAGTTTTGATTTATT
AGCATCAAAATATCAACAAGAATTAACTAATCTGATTGTTAAATTAAATGATGAGCATGAAATAATTAATGTTGATTATG
ATATTAATTTATTAAAGGCAATTAGTCCAGTTTATAAATTAATCGCTTTTAGTGAGGCTTATAGTTCATACTCTAATTTT
ACAAACATCACTTTTGGCAAACATGATATTGATTATGATAACTATGAGGATTTAATTATTAAAACGCGAACTTTATATTT
AGGAACACAATTAAAACAACGTTTTATGATAGGTGCTTATGTTACTCAAGAAAGCCAGTTTGAAACTTTATTAGTTGCTG
CTAAAAAAATGCGTACATTAATAGTTGATCAAGCTAAAAATTTATTTAAAAATTGTGATTTAATTTTAAATTTAGCAGTT
CATGATATAAGTGAAACAATTGATGATGTTAACAACCAAAAGCCCACAACTAATTTAGTTGAAGATGTAATGCAAATTGC
TAATTTTGGTGGTTTTCCATCAATTGTAATCCCCTTTTTAACAACTAAATTAGGAAATTTAGGTTTAAATTTGTGAGCAG
ATTACTTAAATGATAATAAATTATTAAATGGCGCTTATTTAATCAATAATTTACTAGGAAATGAGGAAAAATAA

Upstream 100 bases:

>100_bases
TTTTTACGAGAAGATGAAATTAATTCTTGTGTAGGTAATAATAGCGAAGAAGTTCTAAATAATTGCCTAAAAGTAATCGA
TCATTATGGGGTATTAAAAA

Downstream 100 bases:

>100_bases
TAATGCAAAATTTTGAAGTTATTATTGGTATTGAAGTGCATACTGCACTTAATACAAAAACCAAAATGTTTTCTAATGCA
GCAACATCGCATAAGAGTAT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MKNKTILALHNQIKKREVKIYDLIKKSIDHDLKIKNLNATLANNYEQALKQAAFLDEKISSDNLEIDYLFGIPYSLKDNI
STKNIITTGGSLFLKNYNPPYDATVKTLLDQKQAILLNKSNLDEFGLGGTGTDSAFGPVINPLNPKHVSGGSSSGSAVLV
QQGVVPFAIATDTGDSVRRPASIMGIVGFKPSYGLISRYGVYPFAPSLDHVGIFTNNIFDTQIVFNALAKRDFKDFTSLS
FKTQSLSMLKTKNKYKIGVLKPSFDLLASKYQQELTNLIVKLNDEHEIINVDYDINLLKAISPVYKLIAFSEAYSSYSNF
TNITFGKHDIDYDNYEDLIIKTRTLYLGTQLKQRFMIGAYVTQESQFETLLVAAKKMRTLIVDQAKNLFKNCDLILNLAV
HDISETIDDVNNQKPTTNLVEDVMQIANFGGFPSIVIPFLTTKLGNLGLNLWADYLNDNKLLNGAYLINNLLGNEEK

Sequences:

>Translated_477_residues
MKNKTILALHNQIKKREVKIYDLIKKSIDHDLKIKNLNATLANNYEQALKQAAFLDEKISSDNLEIDYLFGIPYSLKDNI
STKNIITTGGSLFLKNYNPPYDATVKTLLDQKQAILLNKSNLDEFGLGGTGTDSAFGPVINPLNPKHVSGGSSSGSAVLV
QQGVVPFAIATDTGDSVRRPASIMGIVGFKPSYGLISRYGVYPFAPSLDHVGIFTNNIFDTQIVFNALAKRDFKDFTSLS
FKTQSLSMLKTKNKYKIGVLKPSFDLLASKYQQELTNLIVKLNDEHEIINVDYDINLLKAISPVYKLIAFSEAYSSYSNF
TNITFGKHDIDYDNYEDLIIKTRTLYLGTQLKQRFMIGAYVTQESQFETLLVAAKKMRTLIVDQAKNLFKNCDLILNLAV
HDISETIDDVNNQKPTTNLVEDVMQIANFGGFPSIVIPFLTTKLGNLGLNL*ADYLNDNKLLNGAYLINNLLGNEEK
>Mature_477_residues
MKNKTILALHNQIKKREVKIYDLIKKSIDHDLKIKNLNATLANNYEQALKQAAFLDEKISSDNLEIDYLFGIPYSLKDNI
STKNIITTGGSLFLKNYNPPYDATVKTLLDQKQAILLNKSNLDEFGLGGTGTDSAFGPVINPLNPKHVSGGSSSGSAVLV
QQGVVPFAIATDTGDSVRRPASIMGIVGFKPSYGLISRYGVYPFAPSLDHVGIFTNNIFDTQIVFNALAKRDFKDFTSLS
FKTQSLSMLKTKNKYKIGVLKPSFDLLASKYQQELTNLIVKLNDEHEIINVDYDINLLKAISPVYKLIAFSEAYSSYSNF
TNITFGKHDIDYDNYEDLIIKTRTLYLGTQLKQRFMIGAYVTQESQFETLLVAAKKMRTLIVDQAKNLFKNCDLILNLAV
HDISETIDDVNNQKPTTNLVEDVMQIANFGGFPSIVIPFLTTKLGNLGLNL*ADYLNDNKLLNGAYLINNLLGNEEK

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=486, Percent_Identity=32.9218106995885, Blast_Score=178, Evalue=8e-45,
Organism=Homo sapiens, GI195972892, Length=209, Percent_Identity=28.2296650717703, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17543272, Length=466, Percent_Identity=28.1115879828326, Blast_Score=154, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI17537465, Length=199, Percent_Identity=28.643216080402, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71990152, Length=149, Percent_Identity=34.8993288590604, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17556264, Length=247, Percent_Identity=24.2914979757085, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17538252, Length=147, Percent_Identity=31.9727891156463, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=397, Percent_Identity=35.0125944584383, Blast_Score=187, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6319685, Length=173, Percent_Identity=40.4624277456647, Blast_Score=107, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24648113, Length=462, Percent_Identity=30.0865800865801, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI21356731, Length=204, Percent_Identity=29.9019607843137, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24644968, Length=211, Percent_Identity=28.9099526066351, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI45550774, Length=198, Percent_Identity=29.7979797979798, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24648435, Length=198, Percent_Identity=29.7979797979798, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24648437, Length=198, Percent_Identity=29.7979797979798, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24652985, Length=210, Percent_Identity=29.0476190476191, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19922090, Length=210, Percent_Identity=29.0476190476191, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24652981, Length=210, Percent_Identity=29.0476190476191, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24652983, Length=210, Percent_Identity=29.0476190476191, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI161078093, Length=218, Percent_Identity=29.3577981651376, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24648441, Length=139, Percent_Identity=32.3741007194245, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24648439, Length=139, Percent_Identity=32.3741007194245, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53046; Mature: 53046

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKTILALHNQIKKREVKIYDLIKKSIDHDLKIKNLNATLANNYEQALKQAAFLDEKIS
CCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHCCHHHHHHHHHHHHHHCC
SDNLEIDYLFGIPYSLKDNISTKNIITTGGSLFLKNYNPPYDATVKTLLDQKQAILLNKS
CCCEEEEEEECCCCCCCCCCCCCEEEECCCEEEEECCCCCHHHHHHHHHCCCCEEEEECC
NLDEFGLGGTGTDSAFGPVINPLNPKHVSGGSSSGSAVLVQQGVVPFAIATDTGDSVRRP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEEECCCCHHHCC
ASIMGIVGFKPSYGLISRYGVYPFAPSLDHVGIFTNNIFDTQIVFNALAKRDFKDFTSLS
HHHEEEECCCCCCCHHHHCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCE
FKTQSLSMLKTKNKYKIGVLKPSFDLLASKYQQELTNLIVKLNDEHEIINVDYDINLLKA
ECHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHEEECCCCEEEEECCCHHHHHH
ISPVYKLIAFSEAYSSYSNFTNITFGKHDIDYDNYEDLIIKTRTLYLGTQLKQRFMIGAY
HHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHEE
VTQESQFETLLVAAKKMRTLIVDQAKNLFKNCDLILNLAVHDISETIDDVNNQKPTTNLV
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHHHHHCCCCCCCHHHH
EDVMQIANFGGFPSIVIPFLTTKLGNLGLNLADYLNDNKLLNGAYLINNLLGNEEK
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCEECHHHHHHHHHCCCCC
>Mature Secondary Structure
MKNKTILALHNQIKKREVKIYDLIKKSIDHDLKIKNLNATLANNYEQALKQAAFLDEKIS
CCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHCCHHHHHHHHHHHHHHCC
SDNLEIDYLFGIPYSLKDNISTKNIITTGGSLFLKNYNPPYDATVKTLLDQKQAILLNKS
CCCEEEEEEECCCCCCCCCCCCCEEEECCCEEEEECCCCCHHHHHHHHHCCCCEEEEECC
NLDEFGLGGTGTDSAFGPVINPLNPKHVSGGSSSGSAVLVQQGVVPFAIATDTGDSVRRP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEEECCCCHHHCC
ASIMGIVGFKPSYGLISRYGVYPFAPSLDHVGIFTNNIFDTQIVFNALAKRDFKDFTSLS
HHHEEEECCCCCCCHHHHCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCE
FKTQSLSMLKTKNKYKIGVLKPSFDLLASKYQQELTNLIVKLNDEHEIINVDYDINLLKA
ECHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHEEECCCCEEEEECCCHHHHHH
ISPVYKLIAFSEAYSSYSNFTNITFGKHDIDYDNYEDLIIKTRTLYLGTQLKQRFMIGAY
HHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHEE
VTQESQFETLLVAAKKMRTLIVDQAKNLFKNCDLILNLAVHDISETIDDVNNQKPTTNLV
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHHHHHCCCCCCCHHHH
EDVMQIANFGGFPSIVIPFLTTKLGNLGLNLADYLNDNKLLNGAYLINNLLGNEEK
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCEECHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7569993 [H]