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The map label for this gene is fmt

Identifier: 13358026

GI number: 13358026

Start: 526944

End: 527861

Strand: Reverse

Name: fmt

Synonym: UU463

Alternate gene names: 13358026

Gene position: 527861-526944 (Counterclockwise)

Preceding gene: 13358027

Following gene: 13358025

Centisome position: 70.22

GC content: 27.12

Gene sequence:

>918_bases
ATGAAATATAAAGTAATGTTTTTTGGTACACCAGAAATCGCTAAAATTGTTTTGGAAACATTGTTTAACATGCATGAAGT
TGATTTAATCGCTGTTGTTAGTCAACCAGATGCACATTTTGATCGAAAAAAGAATGTTATCTACTCTCCTGTTAAACAGT
TTTGTTTAGATCATAATATTAAGTTATTTCAACCAGAAAAAATCAAAGAAATTGAAGAAGAAATCCGTATTTTAGGACCA
GATATTATCATTACTTGTGCTTTTGGTCAATTTATTAATCAAGGGATAATTGATATTCCTAAATACAAAATCGTTAATAT
TCATGCTTCACTTTTACCAAAATTACGTGGCGGAGCACCAATTCATTATGCCATTTTAAATGGTGAATTAAAAACAGGAA
TCACTTTAATGCATACGATTAAAAAAATGGATGCAGGTAATATTTTATTTCAAAGAAGTTTAGAAATAAACGATTGCACA
ACAACCAAATCACTAACTTTAGAGTTAGCTAATCTTAGTGCATTGATGATTAAAGAACATTTTTTAGAATTAGTAAAACC
AAATTTAGTTGGAATTCAACAAGATGAAAATTCTGTTAGTTTTGCTTATAATATTCAAAAGGACCAAAATATTATTAATT
TTGACCAACCAGCTTTTTTTATTAATCGTTTTGTTAATGCTATGTATGATAAACCAATCGCTATTATGCAATATAATGGC
GTTTCAATTAAAGTATATCAAGTTAAAATCACTAATCAAAAGTCATGTCAAAAACCTGGAACAATCATGATTTTTAAAAA
TCAACTTTTTGTAAGTACGCAAGATTTTGATATTGAACTATTATTGATTCAGCTTCCAAATAAAAAACCTTTATCTCCAA
AAGTTTTATTGAATGGGAAAAACCCTTTTATTAATTAA

Upstream 100 bases:

>100_bases
GAAAAACAAAAAAATAAAACAAACTAATAACTCCATTTTAAGGAGTTATTTTGCTATCTTTACCTTATTTATAAGGTATT
AAAATAAGGAATTTAATTAA

Downstream 100 bases:

>100_bases
AATATAGTAAATTGTTATAATTTAATCATATTGATTACCAACCATTTATTAAAGAAAGGACAGTAAATGAATTTAAGGCA
TTCAAAAACTACTTGATTTA

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MKYKVMFFGTPEIAKIVLETLFNMHEVDLIAVVSQPDAHFDRKKNVIYSPVKQFCLDHNIKLFQPEKIKEIEEEIRILGP
DIIITCAFGQFINQGIIDIPKYKIVNIHASLLPKLRGGAPIHYAILNGELKTGITLMHTIKKMDAGNILFQRSLEINDCT
TTKSLTLELANLSALMIKEHFLELVKPNLVGIQQDENSVSFAYNIQKDQNIINFDQPAFFINRFVNAMYDKPIAIMQYNG
VSIKVYQVKITNQKSCQKPGTIMIFKNQLFVSTQDFDIELLLIQLPNKKPLSPKVLLNGKNPFIN

Sequences:

>Translated_305_residues
MKYKVMFFGTPEIAKIVLETLFNMHEVDLIAVVSQPDAHFDRKKNVIYSPVKQFCLDHNIKLFQPEKIKEIEEEIRILGP
DIIITCAFGQFINQGIIDIPKYKIVNIHASLLPKLRGGAPIHYAILNGELKTGITLMHTIKKMDAGNILFQRSLEINDCT
TTKSLTLELANLSALMIKEHFLELVKPNLVGIQQDENSVSFAYNIQKDQNIINFDQPAFFINRFVNAMYDKPIAIMQYNG
VSIKVYQVKITNQKSCQKPGTIMIFKNQLFVSTQDFDIELLLIQLPNKKPLSPKVLLNGKNPFIN
>Mature_305_residues
MKYKVMFFGTPEIAKIVLETLFNMHEVDLIAVVSQPDAHFDRKKNVIYSPVKQFCLDHNIKLFQPEKIKEIEEEIRILGP
DIIITCAFGQFINQGIIDIPKYKIVNIHASLLPKLRGGAPIHYAILNGELKTGITLMHTIKKMDAGNILFQRSLEINDCT
TTKSLTLELANLSALMIKEHFLELVKPNLVGIQQDENSVSFAYNIQKDQNIINFDQPAFFINRFVNAMYDKPIAIMQYNG
VSIKVYQVKITNQKSCQKPGTIMIFKNQLFVSTQDFDIELLLIQLPNKKPLSPKVLLNGKNPFIN

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI164663775, Length=221, Percent_Identity=27.6018099547511, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI238814322, Length=222, Percent_Identity=26.5765765765766, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1789683, Length=303, Percent_Identity=27.7227722772277, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1788589, Length=244, Percent_Identity=25, Blast_Score=98, Evalue=7e-22,
Organism=Drosophila melanogaster, GI28571984, Length=231, Percent_Identity=29.8701298701299, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI45550868, Length=231, Percent_Identity=29.8701298701299, Blast_Score=92, Evalue=6e-19,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_UREP2 (B1AJA4)

Other databases:

- EMBL:   CP000942
- RefSeq:   YP_001752548.1
- ProteinModelPortal:   B1AJA4
- SMR:   B1AJA4
- GeneID:   6155492
- GenomeReviews:   CP000942_GR
- KEGG:   upa:UPA3_0482
- HOGENOM:   HBG571560
- OMA:   QIRACTP
- ProtClustDB:   CLSK2298953
- BioCyc:   UPAR505682:UPA3_0482-MONOMER
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 34794; Mature: 34794

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYKVMFFGTPEIAKIVLETLFNMHEVDLIAVVSQPDAHFDRKKNVIYSPVKQFCLDHNI
CEEEEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCC
KLFQPEKIKEIEEEIRILGPDIIITCAFGQFINQGIIDIPKYKIVNIHASLLPKLRGGAP
EEECCHHHHHHHHHHHEECCCEEEEEEHHHHHCCCCEECCCEEEEEEEHHHHHHHCCCCC
IHYAILNGELKTGITLMHTIKKMDAGNILFQRSLEINDCTTTKSLTLELANLSALMIKEH
EEEEEECCCHHHCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEEEECHHHHHHHHH
FLELVKPNLVGIQQDENSVSFAYNIQKDQNIINFDQPAFFINRFVNAMYDKPIAIMQYNG
HHHHHCCCEEEEEECCCCEEEEEEECCCCCEECCCCHHHHHHHHHHHHHCCCEEEEEECC
VSIKVYQVKITNQKSCQKPGTIMIFKNQLFVSTQDFDIELLLIQLPNKKPLSPKVLLNGK
EEEEEEEEEECCCCCCCCCCEEEEEECEEEEEECCCCEEEEEEECCCCCCCCCEEEECCC
NPFIN
CCCCC
>Mature Secondary Structure
MKYKVMFFGTPEIAKIVLETLFNMHEVDLIAVVSQPDAHFDRKKNVIYSPVKQFCLDHNI
CEEEEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCC
KLFQPEKIKEIEEEIRILGPDIIITCAFGQFINQGIIDIPKYKIVNIHASLLPKLRGGAP
EEECCHHHHHHHHHHHEECCCEEEEEEHHHHHCCCCEECCCEEEEEEEHHHHHHHCCCCC
IHYAILNGELKTGITLMHTIKKMDAGNILFQRSLEINDCTTTKSLTLELANLSALMIKEH
EEEEEECCCHHHCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEEEECHHHHHHHHH
FLELVKPNLVGIQQDENSVSFAYNIQKDQNIINFDQPAFFINRFVNAMYDKPIAIMQYNG
HHHHHCCCEEEEEECCCCEEEEEEECCCCCEECCCCHHHHHHHHHHHHHCCCEEEEEECC
VSIKVYQVKITNQKSCQKPGTIMIFKNQLFVSTQDFDIELLLIQLPNKKPLSPKVLLNGK
EEEEEEEEEECCCCCCCCCCEEEEEECEEEEEECCCCEEEEEEECCCCCCCCCEEEECCC
NPFIN
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA