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The map label for this gene is nadE

Identifier: 13358023

GI number: 13358023

Start: 523974

End: 524741

Strand: Reverse

Name: nadE

Synonym: UU460

Alternate gene names: 13358023

Gene position: 524741-523974 (Counterclockwise)

Preceding gene: 13358024

Following gene: 13358022

Centisome position: 69.81

GC content: 23.31

Gene sequence:

>768_bases
ATGAATAATGATTTGATTAAATATCTACAATGGTTGAAAAATGATTTTATAAAATTTTTAAATGACGCACAAACGAATAA
TGTTATTTTGGGCATTTCAGGTGGTATTGATAGCACTTTAACATTAGCTATTTTAAATGATTTAAAAAAAGAGTATGATC
TTAATATTTATGCTTATTTTTTAGATATTCATAATTCGAGTTTAGATTATGAATGTATTAATGAATTAAAAAATATTTAT
CCTAACATTGAAGTTATCAATCTTGTTGATATCTATGATAGCTATTGTAAAATGATTAATAAAAAAACAAATGATAAATA
TGTTCTTTATAATTTAAAGCCTAAAATTCGTACAAATTATTTATATGCAATGGCGAATGCATACAAGGGTGTTGTTGTGA
GTAATTTAAACTATGATGAGTACATTTTAGGTTTTTTTACTAAATATGGCGATAGTGCCGCTGATTATTATATGTTAATT
GGTTTACTAAAAAAACATATTTATGAATTAGGTGCTTATTATCATTTACCAAATAAAATTTTAAACCGTGCTCCTACTCC
AGCAAATGAAGATGATGAACACAAAACTGATGAGAGTTTTTTTGGTTTTACATATAACGATCTTGATCAATTTTTACTTT
ATCGTAAAATTAATCCTAAAATTGTTAGCATGATTAAAAAACGTTATGAGACTAATGCTCATAAACATTTTGTTTTTGAT
AAAAAGAAATTATTTTTAAATTATAAATTAGGAAATAAACATGAATAA

Upstream 100 bases:

>100_bases
AGCATCAATTTTAACCAGCAATTACTACAAGCTGGAGCAGTAGCGGGGGATAGTATTAAAATATATGATATTACATTAGA
ATTTGAAGAGTAAATAAATT

Downstream 100 bases:

>100_bases
CAAAAAAGGTAAAAAATTTTATGATTTTTTAAAAACAATCGAGGGTTTTAAAATTATTACGATTATTTCATATGTTTTTG
TAGCAATTTTATTAGCAATT

Product: NH(3)-dependent NAD(+) synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MNNDLIKYLQWLKNDFIKFLNDAQTNNVILGISGGIDSTLTLAILNDLKKEYDLNIYAYFLDIHNSSLDYECINELKNIY
PNIEVINLVDIYDSYCKMINKKTNDKYVLYNLKPKIRTNYLYAMANAYKGVVVSNLNYDEYILGFFTKYGDSAADYYMLI
GLLKKHIYELGAYYHLPNKILNRAPTPANEDDEHKTDESFFGFTYNDLDQFLLYRKINPKIVSMIKKRYETNAHKHFVFD
KKKLFLNYKLGNKHE

Sequences:

>Translated_255_residues
MNNDLIKYLQWLKNDFIKFLNDAQTNNVILGISGGIDSTLTLAILNDLKKEYDLNIYAYFLDIHNSSLDYECINELKNIY
PNIEVINLVDIYDSYCKMINKKTNDKYVLYNLKPKIRTNYLYAMANAYKGVVVSNLNYDEYILGFFTKYGDSAADYYMLI
GLLKKHIYELGAYYHLPNKILNRAPTPANEDDEHKTDESFFGFTYNDLDQFLLYRKINPKIVSMIKKRYETNAHKHFVFD
KKKLFLNYKLGNKHE
>Mature_255_residues
MNNDLIKYLQWLKNDFIKFLNDAQTNNVILGISGGIDSTLTLAILNDLKKEYDLNIYAYFLDIHNSSLDYECINELKNIY
PNIEVINLVDIYDSYCKMINKKTNDKYVLYNLKPKIRTNYLYAMANAYKGVVVSNLNYDEYILGFFTKYGDSAADYYMLI
GLLKKHIYELGAYYHLPNKILNRAPTPANEDDEHKTDESFFGFTYNDLDQFLLYRKINPKIVSMIKKRYETNAHKHFVFD
KKKLFLNYKLGNKHE

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788036, Length=230, Percent_Identity=29.5652173913043, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 30078; Mature: 30078

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNDLIKYLQWLKNDFIKFLNDAQTNNVILGISGGIDSTLTLAILNDLKKEYDLNIYAYF
CCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCEEEEEE
LDIHNSSLDYECINELKNIYPNIEVINLVDIYDSYCKMINKKTNDKYVLYNLKPKIRTNY
EEECCCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHH
LYAMANAYKGVVVSNLNYDEYILGFFTKYGDSAADYYMLIGLLKKHIYELGAYYHLPNKI
HHHHHHHHCCEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LNRAPTPANEDDEHKTDESFFGFTYNDLDQFLLYRKINPKIVSMIKKRYETNAHKHFVFD
HHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEE
KKKLFLNYKLGNKHE
CEEEEEEEECCCCCC
>Mature Secondary Structure
MNNDLIKYLQWLKNDFIKFLNDAQTNNVILGISGGIDSTLTLAILNDLKKEYDLNIYAYF
CCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCEEEEEE
LDIHNSSLDYECINELKNIYPNIEVINLVDIYDSYCKMINKKTNDKYVLYNLKPKIRTNY
EEECCCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHH
LYAMANAYKGVVVSNLNYDEYILGFFTKYGDSAADYYMLIGLLKKHIYELGAYYHLPNKI
HHHHHHHHCCEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LNRAPTPANEDDEHKTDESFFGFTYNDLDQFLLYRKINPKIVSMIKKRYETNAHKHFVFD
HHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEE
KKKLFLNYKLGNKHE
CEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7569993 [H]