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The map label for this gene is proS

Identifier: 13358015

GI number: 13358015

Start: 512663

End: 514081

Strand: Reverse

Name: proS

Synonym: UU452

Alternate gene names: 13358015

Gene position: 514081-512663 (Counterclockwise)

Preceding gene: 13358016

Following gene: 13358014

Centisome position: 68.39

GC content: 28.26

Gene sequence:

>1419_bases
ATGAGTAAAAAATTAGAAAAGATTACAACAAGAAAAGAAAATTTTGCTGATTGATATACAAGTATTGTAAATAATGCAAA
ATTAATTCAGTACACTGATATTAAAGGAATGATGGTTTTTCAACCAAATGCTTGAGCAATTTGAGAAGCAATTAAAAACC
AAATTGATGTCGAATTTAAAAAACACGGAGTACGCAATCTTGCCATGCCAACTTTAATTCCTTTAAGTGAATTTCAAAAA
GAAAAAGATCATATTGAAGGTTTTGCTCCTGAATTATTTATGGTTAATCAAATTGGTGATAAAAAATTAGATAATTCTTA
TGCAATACGTCCTACGAGTGAAATTTTATTTTGTAATTATTTTAAAAATATTGTTAATAGTTATAATGATTTACCGATTA
AAAATAATCAATGGTGTTCAGTAATGCGTGCTGAAAAAACAACACGTCCATTTCTTCGAAATGCTGAGTTTCATTGACAA
GAATTACATGCTATTTTTGCAAGTGAAAATGAGGCCGATTCTTTTGCTAAAGTAATTTTAGATGTGTATACAGATTTTGT
TCAAAATTATTTGTGTATACCTGTTATTAAAGGATTAAAAACACCATGAGAACGTTTTGCTGGTGCTCAAAAAACATATA
CAATTGAAGCTATGATGCAAGATGGCCAAGCATTACAATCTGCAACAAGTCATTATTTAGGGCAGTTTTTTGCAAAAGCT
TATGATATTAAGTTTCAAGGTCAAGATAATCAAATGCATTATGTACATCAAATGTCTGCTGGATTATCAACACGAATTAT
TGGAGCATTAATTATGGTTCATGCTGATGATCAGGGTTTGATTTTGCCCCCTGATATTGCTTTTAATCAAATTGCTATTT
TAACGATTTTTTCTAATAAAAATCCCCAATTATTAAAAATTAGTCAACAGATTTGTGAACAATTAAATAATTATCGTTTG
TTTGAAGATCATAGTGATAAAGGAATTGGTTATAAATTAGCACAACAAGAAATTGAGGGAACGCCTATTTGTATATTAGT
TGGTGCTAAAGAATTAGTTAATCAACAAGTTGTTATTGTGCGTCGTGATACTCATGAAAAAATTAATGTTGATTTAACTG
ATTTGAAACTAATCATCCCAAAATTATTAGCAGATATTAAAAGAAATATTTATGAAAAGGCAAAAAAAGATTTAGAGGAT
TCGATTGTTTTTGTAAATAATATCGAGGAATTAAAACAAGTTATTGCACAAAATAAAATGGCTAAGGCATTCTTTGATGG
TACAAAAGAAGATGATGAACAAATTAAATTATTAACTAATGCTTCAACACGTTGTATTTTTGATGAAACTCAAAACGGGC
AGTGTTTTTATACAAATAAAAAAACAAACAAATTGACTTTATTTGCACGTGCTTATTAG

Upstream 100 bases:

>100_bases
TGTGCCCGTATTGGCGCAAGTGCTTTAAAAATGTGTTTAAAGCAATTTACAGATAAACACAAAACAATTGTTTAATAAAA
GTTTAGGTTGGATTGATAAA

Downstream 100 bases:

>100_bases
ATTTAATAAATGAAATAAAAAAGTTTATTTTCATATATAATTTAAGTTAGTTAAAGAAGTTAAAATTATAAAAATAATTA
ATTTTGGAGTTGAATTATGG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MSKKLEKITTRKENFADWYTSIVNNAKLIQYTDIKGMMVFQPNAWAIWEAIKNQIDVEFKKHGVRNLAMPTLIPLSEFQK
EKDHIEGFAPELFMVNQIGDKKLDNSYAIRPTSEILFCNYFKNIVNSYNDLPIKNNQWCSVMRAEKTTRPFLRNAEFHWQ
ELHAIFASENEADSFAKVILDVYTDFVQNYLCIPVIKGLKTPWERFAGAQKTYTIEAMMQDGQALQSATSHYLGQFFAKA
YDIKFQGQDNQMHYVHQMSAGLSTRIIGALIMVHADDQGLILPPDIAFNQIAILTIFSNKNPQLLKISQQICEQLNNYRL
FEDHSDKGIGYKLAQQEIEGTPICILVGAKELVNQQVVIVRRDTHEKINVDLTDLKLIIPKLLADIKRNIYEKAKKDLED
SIVFVNNIEELKQVIAQNKMAKAFFDGTKEDDEQIKLLTNASTRCIFDETQNGQCFYTNKKTNKLTLFARAY

Sequences:

>Translated_472_residues
MSKKLEKITTRKENFAD*YTSIVNNAKLIQYTDIKGMMVFQPNA*AI*EAIKNQIDVEFKKHGVRNLAMPTLIPLSEFQK
EKDHIEGFAPELFMVNQIGDKKLDNSYAIRPTSEILFCNYFKNIVNSYNDLPIKNNQWCSVMRAEKTTRPFLRNAEFH*Q
ELHAIFASENEADSFAKVILDVYTDFVQNYLCIPVIKGLKTP*ERFAGAQKTYTIEAMMQDGQALQSATSHYLGQFFAKA
YDIKFQGQDNQMHYVHQMSAGLSTRIIGALIMVHADDQGLILPPDIAFNQIAILTIFSNKNPQLLKISQQICEQLNNYRL
FEDHSDKGIGYKLAQQEIEGTPICILVGAKELVNQQVVIVRRDTHEKINVDLTDLKLIIPKLLADIKRNIYEKAKKDLED
SIVFVNNIEELKQVIAQNKMAKAFFDGTKEDDEQIKLLTNASTRCIFDETQNGQCFYTNKKTNKLTLFARAY
>Mature_471_residues
SKKLEKITTRKENFAD*YTSIVNNAKLIQYTDIKGMMVFQPNA*AI*EAIKNQIDVEFKKHGVRNLAMPTLIPLSEFQKE
KDHIEGFAPELFMVNQIGDKKLDNSYAIRPTSEILFCNYFKNIVNSYNDLPIKNNQWCSVMRAEKTTRPFLRNAEFH*QE
LHAIFASENEADSFAKVILDVYTDFVQNYLCIPVIKGLKTP*ERFAGAQKTYTIEAMMQDGQALQSATSHYLGQFFAKAY
DIKFQGQDNQMHYVHQMSAGLSTRIIGALIMVHADDQGLILPPDIAFNQIAILTIFSNKNPQLLKISQQICEQLNNYRLF
EDHSDKGIGYKLAQQEIEGTPICILVGAKELVNQQVVIVRRDTHEKINVDLTDLKLIIPKLLADIKRNIYEKAKKDLEDS
IVFVNNIEELKQVIAQNKMAKAFFDGTKEDDEQIKLLTNASTRCIFDETQNGQCFYTNKKTNKLTLFARAY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro) [H]

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI62241042, Length=497, Percent_Identity=35.8148893360161, Blast_Score=278, Evalue=7e-75,
Organism=Caenorhabditis elegans, GI71991342, Length=489, Percent_Identity=33.9468302658487, Blast_Score=264, Evalue=7e-71,
Organism=Caenorhabditis elegans, GI71991346, Length=357, Percent_Identity=37.5350140056022, Blast_Score=238, Evalue=4e-63,
Organism=Saccharomyces cerevisiae, GI6321808, Length=432, Percent_Identity=35.1851851851852, Blast_Score=259, Evalue=7e-70,
Organism=Drosophila melanogaster, GI24649468, Length=496, Percent_Identity=36.491935483871, Blast_Score=270, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24649466, Length=496, Percent_Identity=36.491935483871, Blast_Score=270, Evalue=1e-72,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 53349; Mature: 53218

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKLEKITTRKENFADYTSIVNNAKLIQYTDIKGMMVFQPNAAIEAIKNQIDVEFKKHG
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEECCCHHHHHHHHHHCHHHHHCC
VRNLAMPTLIPLSEFQKEKDHIEGFAPELFMVNQIGDKKLDNSYAIRPTSEILFCNYFKN
CHHCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEECCCHHHEEHHHHHH
IVNSYNDLPIKNNQWCSVMRAEKTTRPFLRNAEFHQELHAIFASENEADSFAKVILDVYT
HHCCCCCCCCCCCCHHHHHHHHHHHCHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
DFVQNYLCIPVIKGLKTPERFAGAQKTYTIEAMMQDGQALQSATSHYLGQFFAKAYDIKF
HHHHCCCHHHHHHCCCCHHHHCCCCCEEEHHHHHHCCHHHHHHHHHHHHHHHHHHEEEEE
QGQDNQMHYVHQMSAGLSTRIIGALIMVHADDQGLILPPDIAFNQIAILTIFSNKNPQLL
CCCCCCCHHHHHHHCCHHHHHEEEEEEEEECCCCCEECCCCCCCCEEEEEEECCCCCCCH
KISQQICEQLNNYRLFEDHSDKGIGYKLAQQEIEGTPICILVGAKELVNQQVVIVRRDTH
HHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHCCCCCEEEEECCHHHCCCEEEEEEECCC
EKINVDLTDLKLIIPKLLADIKRNIYEKAKKDLEDSIVFVNNIEELKQVIAQNKMAKAFF
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCEEEECCHHHHHHHHHHHHHHHHHH
DGTKEDDEQIKLLTNASTRCIFDETQNGQCFYTNKKTNKLTLFARAY
CCCCCCCCCEEEEECCCCEEEEECCCCCEEEEECCCCCEEEEEEECC
>Mature Secondary Structure 
SKKLEKITTRKENFADYTSIVNNAKLIQYTDIKGMMVFQPNAAIEAIKNQIDVEFKKHG
CCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEECCCHHHHHHHHHHCHHHHHCC
VRNLAMPTLIPLSEFQKEKDHIEGFAPELFMVNQIGDKKLDNSYAIRPTSEILFCNYFKN
CHHCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEECCCHHHEEHHHHHH
IVNSYNDLPIKNNQWCSVMRAEKTTRPFLRNAEFHQELHAIFASENEADSFAKVILDVYT
HHCCCCCCCCCCCCHHHHHHHHHHHCHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
DFVQNYLCIPVIKGLKTPERFAGAQKTYTIEAMMQDGQALQSATSHYLGQFFAKAYDIKF
HHHHCCCHHHHHHCCCCHHHHCCCCCEEEHHHHHHCCHHHHHHHHHHHHHHHHHHEEEEE
QGQDNQMHYVHQMSAGLSTRIIGALIMVHADDQGLILPPDIAFNQIAILTIFSNKNPQLL
CCCCCCCHHHHHHHCCHHHHHEEEEEEEEECCCCCEECCCCCCCCEEEEEEECCCCCCCH
KISQQICEQLNNYRLFEDHSDKGIGYKLAQQEIEGTPICILVGAKELVNQQVVIVRRDTH
HHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHCCCCCEEEEECCHHHCCCEEEEEEECCC
EKINVDLTDLKLIIPKLLADIKRNIYEKAKKDLEDSIVFVNNIEELKQVIAQNKMAKAFF
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCEEEECCHHHHHHHHHHHHHHHHHH
DGTKEDDEQIKLLTNASTRCIFDETQNGQCFYTNKKTNKLTLFARAY
CCCCCCCCCEEEEECCCCEEEEECCCCCEEEEECCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA