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The map label for this gene is thiI

Identifier: 13357980

GI number: 13357980

Start: 482180

End: 483352

Strand: Reverse

Name: thiI

Synonym: UU418

Alternate gene names: 13357980

Gene position: 483352-482180 (Counterclockwise)

Preceding gene: 13357981

Following gene: 13357979

Centisome position: 64.3

GC content: 27.45

Gene sequence:

>1173_bases
ATGAAACCAATAATTTATATTAAATATGGGGAATTAACACTAAAAGGTAAAAATCGAACACAATTTATAAAAGTTTTAGT
GCACAATATCAAACAAATATTAATACAATATCAAAATTTAACATATTGTGTTGGTTATGATAATTTGAAAATTATCAATT
TAGAAAATTATAATTTAAAACAAATTATTGATGATTTGAAAGAAGTTTATGGAATTGCTTTTATTTGTATTGCTTATCAA
GTTGATAAAGAACTTAGTGAAATTCAATTAGCTTGTAAAGAATTTATAAATGATTATGAACAAACATTTAAAATTGAAGC
CCGTCGTAGTGATAAAAGTTTTAAATACAATTCAATGGAAATTAAACAAATGTGCGCTGCTTATTTATTGCAAAATTCAC
CCCTTTTAAAAGTTGATGTTCATCGTCCACAACTATTAATTAATATTGAAATAAAATATGATTGTGCTATAGTTTATGGT
CATAAAATTATGGGTGCTAAAGGATTGCCGGTAGGTATTAATGGTAAGGCCTTAGTGTTACTGTCTGGAGGAATCGATTC
TCCTGTTGCTTCTCGTTTAATTATGAAACGAGGAATTAGTATTGATTTTATTACATTTATTACTCCGCCGCACACTTCAC
AAAAAGCTTTAGATAAAACAATTGCATTAGCAAAACAAATAACATTAGATAATAAATTAACAAAAGCGAATCTATATGTA
TGCAATTTCACTAAATTACAAGACGAAATTGCGCACATTTCTAAAGAATCTTATCGAATTACACTTATGCGTCGTTATTT
TATGCGTATTGCTAAACGTTTAGCAATAAGTGTTAAAGCAAATGCTTTAGTAACTGGGGAGGCACTTGGACAAGTTGCTT
CACAAACTTTAAATAGCATGCAAACAATTAGTAGTGTTTTAGATAATTTTTTAGTTTTAAGACCTTTAATAGCTTACGAT
AAAGAAGAAATAATTAGTTTAGCTAAACGGTTTAATACTTATGAATTATCGATTTTGCCATACGATGATTCATGTAGCTT
GTTTGCACCTAAGAATCCAACAACCAATCCTAATGTTCAAACAGCTGCTAAATTAGAAGAAGAATCATTAGTTTTAGATG
CAATTTATGAATTAGTTTACACTAAAGAAATCACTAAAATTAATTTATCATAA

Upstream 100 bases:

>100_bases
TCAAAAGCAAAAACATGATACGAAGAGTATATTATAAAAGGTATTAGTCCTAAATTAACTAAAGAAGATCTTGAATGATA
TAAGGTGTGAATCAATAAAT

Downstream 100 bases:

>100_bases
AATGGGAGAAAATTATTATGCAAAAAGATTTGTTAACAAAATTAATTGAACAAACATATGGTTTTAATAAAATCACTATT
TTTGTTCATACAAATCCTGA

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MKPIIYIKYGELTLKGKNRTQFIKVLVHNIKQILIQYQNLTYCVGYDNLKIINLENYNLKQIIDDLKEVYGIAFICIAYQ
VDKELSEIQLACKEFINDYEQTFKIEARRSDKSFKYNSMEIKQMCAAYLLQNSPLLKVDVHRPQLLINIEIKYDCAIVYG
HKIMGAKGLPVGINGKALVLLSGGIDSPVASRLIMKRGISIDFITFITPPHTSQKALDKTIALAKQITLDNKLTKANLYV
CNFTKLQDEIAHISKESYRITLMRRYFMRIAKRLAISVKANALVTGEALGQVASQTLNSMQTISSVLDNFLVLRPLIAYD
KEEIISLAKRFNTYELSILPYDDSCSLFAPKNPTTNPNVQTAAKLEEESLVLDAIYELVYTKEITKINLS

Sequences:

>Translated_390_residues
MKPIIYIKYGELTLKGKNRTQFIKVLVHNIKQILIQYQNLTYCVGYDNLKIINLENYNLKQIIDDLKEVYGIAFICIAYQ
VDKELSEIQLACKEFINDYEQTFKIEARRSDKSFKYNSMEIKQMCAAYLLQNSPLLKVDVHRPQLLINIEIKYDCAIVYG
HKIMGAKGLPVGINGKALVLLSGGIDSPVASRLIMKRGISIDFITFITPPHTSQKALDKTIALAKQITLDNKLTKANLYV
CNFTKLQDEIAHISKESYRITLMRRYFMRIAKRLAISVKANALVTGEALGQVASQTLNSMQTISSVLDNFLVLRPLIAYD
KEEIISLAKRFNTYELSILPYDDSCSLFAPKNPTTNPNVQTAAKLEEESLVLDAIYELVYTKEITKINLS
>Mature_390_residues
MKPIIYIKYGELTLKGKNRTQFIKVLVHNIKQILIQYQNLTYCVGYDNLKIINLENYNLKQIIDDLKEVYGIAFICIAYQ
VDKELSEIQLACKEFINDYEQTFKIEARRSDKSFKYNSMEIKQMCAAYLLQNSPLLKVDVHRPQLLINIEIKYDCAIVYG
HKIMGAKGLPVGINGKALVLLSGGIDSPVASRLIMKRGISIDFITFITPPHTSQKALDKTIALAKQITLDNKLTKANLYV
CNFTKLQDEIAHISKESYRITLMRRYFMRIAKRLAISVKANALVTGEALGQVASQTLNSMQTISSVLDNFLVLRPLIAYD
KEEIISLAKRFNTYELSILPYDDSCSLFAPKNPTTNPNVQTAAKLEEESLVLDAIYELVYTKEITKINLS

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1786625, Length=401, Percent_Identity=28.6783042394015, Blast_Score=137, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THII_UREP2 (B1AJ58)

Other databases:

- EMBL:   CP000942
- RefSeq:   YP_001752502.1
- ProteinModelPortal:   B1AJ58
- SMR:   B1AJ58
- GeneID:   6155665
- GenomeReviews:   CP000942_GR
- KEGG:   upa:UPA3_0436
- HOGENOM:   HBG646072
- OMA:   ESIGQVA
- ProtClustDB:   PRK01565
- BioCyc:   UPAR505682:UPA3_0436-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00021
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114
- Gene3D:   G3DSA:3.40.50.620
- SMART:   SM00981
- TIGRFAMs:   TIGR00342

Pfam domain/function: PF02568 ThiI; PF02926 THUMP

EC number: =2.8.1.4

Molecular weight: Translated: 44317; Mature: 44317

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: PS51165 THUMP

Important sites: BINDING 264-264 BINDING 286-286 BINDING 295-295

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPIIYIKYGELTLKGKNRTQFIKVLVHNIKQILIQYQNLTYCVGYDNLKIINLENYNLK
CCCEEEEEECCEEEECCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEEEECCCCHH
QIIDDLKEVYGIAFICIAYQVDKELSEIQLACKEFINDYEQTFKIEARRSDKSFKYNSME
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCEECCHH
IKQMCAAYLLQNSPLLKVDVHRPQLLINIEIKYDCAIVYGHKIMGAKGLPVGINGKALVL
HHHHHHHHHHCCCCEEEEECCCCEEEEEEEEEEEEEEEECCEEECCCCCEECCCCCEEEE
LSGGIDSPVASRLIMKRGISIDFITFITPPHTSQKALDKTIALAKQITLDNKLTKANLYV
EECCCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEE
CNFTKLQDEIAHISKESYRITLMRRYFMRIAKRLAISVKANALVTGEALGQVASQTLNSM
EEHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHEEEEECCEEEEHHHHHHHHHHHHHHH
QTISSVLDNFLVLRPLIAYDKEEIISLAKRFNTYELSILPYDDSCSLFAPKNPTTNPNVQ
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEEEECCCCCEECCCCCCCCCCCH
TAAKLEEESLVLDAIYELVYTKEITKINLS
HHHHHHHHHHHHHHHHHHHHHHHHEEEECC
>Mature Secondary Structure
MKPIIYIKYGELTLKGKNRTQFIKVLVHNIKQILIQYQNLTYCVGYDNLKIINLENYNLK
CCCEEEEEECCEEEECCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEEEECCCCHH
QIIDDLKEVYGIAFICIAYQVDKELSEIQLACKEFINDYEQTFKIEARRSDKSFKYNSME
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCEECCHH
IKQMCAAYLLQNSPLLKVDVHRPQLLINIEIKYDCAIVYGHKIMGAKGLPVGINGKALVL
HHHHHHHHHHCCCCEEEEECCCCEEEEEEEEEEEEEEEECCEEECCCCCEECCCCCEEEE
LSGGIDSPVASRLIMKRGISIDFITFITPPHTSQKALDKTIALAKQITLDNKLTKANLYV
EECCCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEE
CNFTKLQDEIAHISKESYRITLMRRYFMRIAKRLAISVKANALVTGEALGQVASQTLNSM
EEHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHEEEEECCEEEEHHHHHHHHHHHHHHH
QTISSVLDNFLVLRPLIAYDKEEIISLAKRFNTYELSILPYDDSCSLFAPKNPTTNPNVQ
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEEEECCCCCEECCCCCCCCCCCH
TAAKLEEESLVLDAIYELVYTKEITKINLS
HHHHHHHHHHHHHHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA