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The map label for this gene is phlc

Identifier: 15004851

GI number: 15004851

Start: 157821

End: 158639

Strand: Direct

Name: phlc

Synonym: CA_P0148

Alternate gene names: 15004851

Gene position: 157821-158639 (Clockwise)

Preceding gene: 15004849

Following gene: 15004853

Centisome position: 82.2

GC content: 34.07

Gene sequence:

>819_bases
ATGAAAAACGTATTCAAGAAGATTACAACTGCTATAATAGCTTCATCCCTTACTCTTACTTTTTCCTTTACAACCTTTGC
TGAAGCCTTACCTCAAAAAACACAAAGTACATATCAAGAGCTTCAAAATACTACGAATAGACGTATCTATGATTTTACAA
GCGATGGCGCAAAAGGAACTCATGAATTTATAACTGTACAAGCTATGACTATACTTGGAAACGACAAAGGGAATAATTTT
AAAAATCTTATTAAAAACTATGAACCCACACTAAGACAGTACTGTGATAAGCCTGATAAAGATGAAAATCAGTGGGCCTT
TGCATATCATTTCTACAATCCTTATACAGGGCTAAACTACCTTCCAAGTATACTTCCTCAGTCTAAAACAACAGCACTTA
CTAAATTCGAAGAACACGCTGCAAATGCAGTATCCAGCTATAGAACGGATAGAACTTATTCTATGCAAGAATTAGGGCGA
GCTTGTCATTTTCTAGAAGATGTAAACGTACCTTACCATTCTGCCAATCTAATAGCAGTATTGTCCACACATTCTCAATA
TGAACAGTTTGTTCAAGATCATCAAACTAGCTATGCTCTAAATAGCACTGATAAATATGGAAACTACTATTCAGAGAACT
TTAATGATTACTGTTACGATATACTAAATGATTGCGCTAAGTACTCTTATTCCTTTAAAGATGAAGTTCAAAAAAGCGAA
TCTTCTTGGGATTCAGTTGCCAATGTAACAGTTAAATATGCTGAAGGCTATGTAGCAGCTTTCTTTTATAGATTTTTACA
TGAGGTTGGAGAAATTTAA

Upstream 100 bases:

>100_bases
AATATACAAAAAAAAGCTTGATTTATTGACATAATATTTCGAATGCCATATACTTTATTTGTGATAATTGTATTAAATTG
TAACCACAGGAGGTCTTTCT

Downstream 100 bases:

>100_bases
TTATTCAGAACAAAAGAGAGCATGGCTCATGCTTAAATTTTAATCATGAGCCATGCTCTCTTTTTTGATTTCATAATTGT
TTATTGTAGATATTCAATTT

Product: phospholipase C

Products: NA

Alternate protein names: PLC; Alpha-toxin; Hemolysin; Lecithinase; Phosphatidylcholine cholinephosphohydrolase [H]

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MKNVFKKITTAIIASSLTLTFSFTTFAEALPQKTQSTYQELQNTTNRRIYDFTSDGAKGTHEFITVQAMTILGNDKGNNF
KNLIKNYEPTLRQYCDKPDKDENQWAFAYHFYNPYTGLNYLPSILPQSKTTALTKFEEHAANAVSSYRTDRTYSMQELGR
ACHFLEDVNVPYHSANLIAVLSTHSQYEQFVQDHQTSYALNSTDKYGNYYSENFNDYCYDILNDCAKYSYSFKDEVQKSE
SSWDSVANVTVKYAEGYVAAFFYRFLHEVGEI

Sequences:

>Translated_272_residues
MKNVFKKITTAIIASSLTLTFSFTTFAEALPQKTQSTYQELQNTTNRRIYDFTSDGAKGTHEFITVQAMTILGNDKGNNF
KNLIKNYEPTLRQYCDKPDKDENQWAFAYHFYNPYTGLNYLPSILPQSKTTALTKFEEHAANAVSSYRTDRTYSMQELGR
ACHFLEDVNVPYHSANLIAVLSTHSQYEQFVQDHQTSYALNSTDKYGNYYSENFNDYCYDILNDCAKYSYSFKDEVQKSE
SSWDSVANVTVKYAEGYVAAFFYRFLHEVGEI
>Mature_272_residues
MKNVFKKITTAIIASSLTLTFSFTTFAEALPQKTQSTYQELQNTTNRRIYDFTSDGAKGTHEFITVQAMTILGNDKGNNF
KNLIKNYEPTLRQYCDKPDKDENQWAFAYHFYNPYTGLNYLPSILPQSKTTALTKFEEHAANAVSSYRTDRTYSMQELGR
ACHFLEDVNVPYHSANLIAVLSTHSQYEQFVQDHQTSYALNSTDKYGNYYSENFNDYCYDILNDCAKYSYSFKDEVQKSE
SSWDSVANVTVKYAEGYVAAFFYRFLHEVGEI

Specific function: Bacterial hemolysins are exotoxins that attack blood cell membranes and cause cell rupture. Constitutes an essential virulence factor in gas gangrene. Binds to eukaryotic membranes where it hydrolyzes both phosphatidylcholine and sphingomyelin, causing ce

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Zn-dependent PLC domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008976
- InterPro:   IPR000907
- InterPro:   IPR001024
- InterPro:   IPR008947
- InterPro:   IPR001531
- ProDom:   PD003946 [H]

Pfam domain/function: PF00882 Zn_dep_PLPC [H]

EC number: =3.1.4.3 [H]

Molecular weight: Translated: 31389; Mature: 31389

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNVFKKITTAIIASSLTLTFSFTTFAEALPQKTQSTYQELQNTTNRRIYDFTSDGAKGT
CHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
HEFITVQAMTILGNDKGNNFKNLIKNYEPTLRQYCDKPDKDENQWAFAYHFYNPYTGLNY
HHHEEEEEEEEECCCCCCHHHHHHHCCCHHHHHHCCCCCCCCCCEEEEEEEECCCCCHHH
LPSILPQSKTTALTKFEEHAANAVSSYRTDRTYSMQELGRACHFLEDVNVPYHSANLIAV
HHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEE
LSTHSQYEQFVQDHQTSYALNSTDKYGNYYSENFNDYCYDILNDCAKYSYSFKDEVQKSE
ECCHHHHHHHHHHHHHHHCCCCCHHCCCHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
SSWDSVANVTVKYAEGYVAAFFYRFLHEVGEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKNVFKKITTAIIASSLTLTFSFTTFAEALPQKTQSTYQELQNTTNRRIYDFTSDGAKGT
CHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
HEFITVQAMTILGNDKGNNFKNLIKNYEPTLRQYCDKPDKDENQWAFAYHFYNPYTGLNY
HHHEEEEEEEEECCCCCCHHHHHHHCCCHHHHHHCCCCCCCCCCEEEEEEEECCCCCHHH
LPSILPQSKTTALTKFEEHAANAVSSYRTDRTYSMQELGRACHFLEDVNVPYHSANLIAV
HHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEE
LSTHSQYEQFVQDHQTSYALNSTDKYGNYYSENFNDYCYDILNDCAKYSYSFKDEVQKSE
ECCHHHHHHHHHHHHHHHCCCCCHHCCCHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
SSWDSVANVTVKYAEGYVAAFFYRFLHEVGEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12009886; 11008117 [H]